FastQCFastQC Report
Thu 26 May 2016
SRR1513056_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513056_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019401
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC155120.7681485747506315No Hit
CCCATGTACTCTGCGTTGATACCAC143950.7128351426982555No Hit
GAGTACATGGGAAGCAGTGGTATCA60500.29959379043587675No Hit
CATGTACTCTGCGTTGATACCACTG43960.21768831450514284No Hit
GTATCAACGCAGAGTACATGGGAAG40680.2014458742963879No Hit
GCGTTGATACCACTGCTTCCCATGT38950.1928789774789653No Hit
GTATCAACGCAGAGTACTTTTTTTT38060.18847172998329703No Hit
ACGCAGAGTACATGGGAAGCAGTGG36680.18163802038327206No Hit
TATCAACGCAGAGTACATGGGAAGC34130.16901051351366073No Hit
GCTTCCCATGTACTCTGCGTTGATA33910.16792108154843935No Hit
ACTCTGCGTTGATACCACTGCTTCC29680.14697427603531937No Hit
TATCAACGCAGAGTACTTTTTTTTT27090.1341486906265769No Hit
GGTATCAACGCAGAGTACTTTTTTT23550.1166187399134694No Hit
GGTATCAACGCAGAGTACATGGGAA23350.11562834721781359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTC350.002190165516.263747
CCGCTCA500.001515154413.2820559
TAGGACC4550.013.1791894
GGACGTG8650.011.7740856
GTCTAGG1850.011.3314531
AGGACGT9150.011.2347185
TAGGACG9300.011.155864
GTAGGAC11050.011.1118643
GCGCCAC951.3705745E-510.99278213
GACGTGG4750.010.9851587
TACACCG1158.60362E-710.7598215
ACCGCTC803.8190722E-410.67307958
GTGCGCC1252.2867607E-710.62432711
ATTATAC1451.4011675E-810.50295453
GACGTGA4700.010.4964567
ACGTGGA5100.010.4172988
CTATACT3150.010.2737244
TGTAGGA13350.010.2037492
CCTATAC2250.010.1528563
AAAGGCG1502.3734174E-810.1528565
Produced by FastQC (version 0.11.5)