FastQCFastQC Report
Thu 26 May 2016
SRR1513055_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513055_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919156
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC40280.43822811361727504No Hit
CCCATGTACTCTGCGTTGATACCAC29050.3160508118317239No Hit
GAGTACATGGGAAGCAGTGGTATCA17230.18745457789537356No Hit
GTATCAACGCAGAGTACTTTTTTTT16790.18266757764731995No Hit
GCTTCCCATGTACTCTGCGTTGATA11960.13011937037891283No Hit
GTATCAACGCAGAGTACATGGGAAG11310.12304766546701539No Hit
GTCCTACAGTGGACATTTCTAAATT11030.12000139258189035No Hit
GGTATCAACGCAGAGTACTTTTTTT10730.11673752877639922No Hit
GTCCTACAGTGTGCATTTCTCATTT10450.11369125589127417No Hit
CATGTACTCTGCGTTGATACCACTG9690.10542280091736331No Hit
TATCAACGCAGAGTACTTTTTTTTT9470.1030293007933365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG402.7605405E-416.6242395
GCGTTTA651.9129584E-716.07531213
GCCGGTT653.3701017E-614.61471611
AAACCGA400.005278534314.2493486
GTACTAG759.553223E-713.9471141
GTCCTAC7300.013.6778991
GTCTTAC1051.3478711E-913.5848521
TGCGTTT1001.0057192E-813.2993912
CCGGTTT751.4777226E-512.66608712
TGTAGAA3700.012.5805042
TAGGACC3700.012.5805044
TAGGACT1457.2759576E-1212.4477064
TCCAACG1155.30963E-912.39141218
CCTAGAC1001.4397483E-712.3501063
CTACACT2000.012.3494334
GTAGGAC6350.012.2678223
TGTAGGA6900.012.2530622
TCCTACA8550.012.1105562
GTGCATT5600.012.0440911
GTGCTAT1209.844371E-911.88674451
Produced by FastQC (version 0.11.5)