##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513055_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	919156
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.098239036681477	31.0	30.0	34.0	23.0	34.0
2	28.830453154850755	31.0	30.0	34.0	16.0	34.0
3	29.155518758513246	31.0	30.0	34.0	22.0	34.0
4	31.981974768156874	35.0	32.0	37.0	18.0	37.0
5	32.228069011136306	35.0	33.0	37.0	19.0	37.0
6	32.646110127116614	36.0	33.0	37.0	25.0	37.0
7	32.64097498139598	37.0	33.0	37.0	25.0	37.0
8	32.610094477977626	37.0	33.0	37.0	25.0	37.0
9	33.95425912467525	39.0	34.0	39.0	23.0	39.0
10	33.45065146721558	38.0	33.0	39.0	17.0	39.0
11	33.8184715108208	39.0	34.0	39.0	23.0	39.0
12	33.638902427879486	39.0	34.0	39.0	19.0	39.0
13	33.732883210249405	39.0	34.0	39.0	22.0	39.0
14	34.02148819134075	39.0	33.0	41.0	11.0	41.0
15	34.578151042913284	40.0	34.0	41.0	17.0	41.0
16	34.166568025449436	39.0	33.0	41.0	16.0	41.0
17	33.72180130467516	39.0	33.0	41.0	10.0	41.0
18	33.147898724482026	39.0	32.0	41.0	10.0	41.0
19	34.025079529481395	39.0	33.0	41.0	17.0	41.0
20	34.27806705281802	39.0	34.0	41.0	16.0	41.0
21	34.2991559648199	39.0	34.0	41.0	11.0	41.0
22	33.892358859649505	39.0	34.0	41.0	8.0	41.0
23	32.25766137630609	39.0	31.0	41.0	2.0	41.0
24	33.33014743960764	39.0	32.0	41.0	2.0	41.0
25	33.071449242566004	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5148611351984087
1101	2	0.8268172688088988
1101	3	0.6421422070958798
1101	4	0.4420922797961353
1101	5	0.7272110759020904
1101	6	0.3717114519546243
1101	7	0.39097385023975306
1101	8	0.47995038551889024
1101	9	0.5587568787431181
1101	10	0.8449218449146585
1101	11	0.5884577161140996
1101	12	0.47970965032519075
1101	13	0.3170808269001171
1101	14	-0.24909580100251105
1101	15	0.25610072781908144
1101	16	0.9545787359492124
1101	17	1.4223611067491007
1101	18	-4.512745476887218
1101	19	-1.037783143717661
1101	20	-0.022176425889107065
1101	21	0.26098898693158645
1101	22	0.8636317459674245
1101	23	2.456288447483331
1101	24	1.5108871181125494
1101	25	1.4907688201916613
1102	1	1.0580563662757108
1102	2	1.355222517091157
1102	3	1.1067888886984747
1102	4	1.6585046171366145
1102	5	1.2922104480644308
1102	6	0.9968841073114021
1102	7	0.9834829759152228
1102	8	0.8898606377774527
1102	9	1.1160178763661506
1102	10	1.4725281973100266
1102	11	1.0275805938575857
1102	12	1.167347747073876
1102	13	0.9014550489872235
1102	14	1.8169246334611486
1102	15	1.3674372730377158
1102	16	1.6867674220942703
1102	17	1.2651764409680126
1102	18	1.5991715428004483
1102	19	1.4255511368795055
1102	20	1.227549858920085
1102	21	1.1605338597008341
1102	22	1.667557493700052
1102	23	3.1020492215080324
1102	24	2.091401240054047
1102	25	2.303460613582402
1103	1	0.8505075155802437
1103	2	1.140419530720326
1103	3	0.8611752959486871
1103	4	1.2192021840568188
1103	5	1.171169980778636
1103	6	0.7033899693948626
1103	7	0.8751438295242266
1103	8	0.7225387064415685
1103	9	0.9810232692027796
1103	10	1.1950142249605733
1103	11	0.9661678107336584
1103	12	1.089306537397583
1103	13	0.9461357510365005
1103	14	1.631190297200277
1103	15	1.0850196013424025
1103	16	1.3017015174284126
1103	17	1.8119927063235934
1103	18	1.851255418919287
1103	19	1.3920729673623455
1103	20	1.2667066153739839
1103	21	1.1627246120365626
1103	22	1.4775363425745596
1103	23	3.0882239494149104
1103	24	1.9228931648064673
1103	25	2.1662438775792694
1104	1	0.6753456224200427
1104	2	1.177200217053194
1104	3	0.8877869600082668
1104	4	1.311783199851611
1104	5	1.017150683723031
1104	6	0.6273141654662311
1104	7	0.7052461288362295
1104	8	0.7563725081276402
1104	9	0.9193889719395258
1104	10	1.2309920208879817
1104	11	0.917527602535877
1104	12	0.9722058023375766
1104	13	0.839985333671919
1104	14	1.7201317277294876
1104	15	1.1001543623440782
1104	16	1.4865872439750163
1104	17	2.0121748793879988
1104	18	2.542805966407485
1104	19	1.6579841787578502
1104	20	0.9479486037735967
1104	21	1.0120497688078345
1104	22	1.4239254121906981
1104	23	2.921734933786233
1104	24	1.869443366221212
1104	25	1.9680705916810695
1105	1	0.6971681235316325
1105	2	1.1152713408745498
1105	3	0.8112288748860621
1105	4	1.1697565565050354
1105	5	1.144659936423416
1105	6	0.6494789782523398
1105	7	0.5320801994494815
1105	8	0.4803733035143978
1105	9	0.6988616642762295
1105	10	1.2089854415415573
1105	11	0.8943986206572916
1105	12	0.6999208639985071
1105	13	0.9874335821068598
1105	14	1.3806888254750334
1105	15	0.8954076756204401
1105	16	1.3398404971226725
1105	17	1.6935998031590387
1105	18	1.6724840721211365
1105	19	1.4368503095757603
1105	20	1.1126933495003755
1105	21	1.0485241589893448
1105	22	1.4767279110131781
1105	23	2.8070362603626506
1105	24	1.7790693920354173
1105	25	1.8639382199147647
1106	1	-0.7558013000330668
1106	2	-0.2964274417162187
1106	3	-0.4681010425421519
1106	4	-2.831236871304739
1106	5	-0.6985599056273486
1106	6	-0.5573622631485122
1106	7	-0.6778528164848012
1106	8	-0.7137826693848517
1106	9	-0.5889978485181615
1106	10	-0.6747046194998205
1106	11	-0.6452451442290013
1106	12	-0.48204734315616804
1106	13	-0.4736210501118592
1106	14	0.28955746949603167
1106	15	-0.2226382639734652
1106	16	-1.1748324158506307
1106	17	-5.876960655258721
1106	18	-7.420055121998022
1106	19	-3.240500220033276
1106	20	-1.814601303081247
1106	21	-1.4083979397570658
1106	22	-1.732470420617112
1106	23	-4.869209350332504
1106	24	-2.3055707625375312
1106	25	-1.1882517125437104
1107	1	0.5424504594022643
1107	2	0.9027475505196705
1107	3	0.621313146229447
1107	4	0.9452521614365992
1107	5	0.9466815969268083
1107	6	0.4242293126101231
1107	7	0.44338184746072784
1107	8	0.5106713407657537
1107	9	0.4564018252343942
1107	10	0.8512667198377599
1107	11	0.6266127637634398
1107	12	0.6359888164512597
1107	13	0.4506172157622643
1107	14	1.5265975942170655
1107	15	0.9211762929356624
1107	16	-5.4453304436115815
1107	17	-0.8703454260446151
1107	18	0.8281178613707851
1107	19	0.5575699468976936
1107	20	0.6113275271018992
1107	21	0.6784177221837311
1107	22	0.6667637955378112
1107	23	2.7173720985486938
1107	24	1.7642886993089562
1107	25	1.6073349705350637
1108	1	0.5269422217410629
1108	2	0.8391413809942136
1108	3	0.6462162102653188
1108	4	1.0730251366503083
1108	5	0.9576445868515364
1108	6	0.6741805286699005
1108	7	0.5370955438679061
1108	8	0.5595144283594706
1108	9	0.6902002489823147
1108	10	1.3301329102922779
1108	11	0.704278313202046
1108	12	0.7365696739364367
1108	13	0.6861506289698198
1108	14	1.0206621305674233
1108	15	0.7646273649185176
1108	16	0.849823516481635
1108	17	1.1744786737273927
1108	18	0.7887924446851642
1108	19	0.9254928632168102
1108	20	0.7671272551251178
1108	21	1.009170814782216
1108	22	1.2365814497633068
1108	23	2.979966696422423
1108	24	1.587755543923258
1108	25	1.8264643415002766
1109	1	-0.06593491391505424
1109	2	0.43455914002761276
1109	3	0.24221403186441748
1109	4	0.3134724687424608
1109	5	-0.131183509454047
1109	6	0.0069008701215764745
1109	7	-0.02977249823096173
1109	8	-0.14407106754336496
1109	9	-0.10502246717964425
1109	10	0.5043948503355935
1109	11	0.03128415182306554
1109	12	0.15345481394935234
1109	13	-0.07033201348113494
1109	14	-1.1093630730244044
1109	15	-0.3290666710757577
1109	16	-0.5028747876585911
1109	17	0.39816245097323133
1109	18	1.1449229967578134
1109	19	0.40995149259735797
1109	20	0.370369526234704
1109	21	0.17475208053446067
1109	22	0.15020785400865577
1109	23	-4.121283351071785
1109	24	-1.4826245363736135
1109	25	-0.2545468418020249
1110	1	0.6303316237250307
1110	2	0.9370086901451522
1110	3	0.7568380220114754
1110	4	1.2790722224189892
1110	5	1.0008576031069865
1110	6	0.8312262908019932
1110	7	0.5965342624687153
1110	8	0.406912515778977
1110	9	0.6833798025152333
1110	10	0.9315026510677242
1110	11	0.7317117967458202
1110	12	0.6848586133912136
1110	13	0.6048377004610686
1110	14	1.455542185297567
1110	15	0.8957328245476006
1110	16	1.229613519307378
1110	17	1.6335084034973164
1110	18	2.2447108848761275
1110	19	1.4705812791658843
1110	20	0.8752177978161129
1110	21	0.8409886585931048
1110	22	1.0219842665809296
1110	23	2.3654884918090247
1110	24	1.5846442543442976
1110	25	2.0023920025303354
1111	1	0.14181494874834044
1111	2	0.3371813696064372
1111	3	0.062451090438177204
1111	4	0.4988286683207406
1111	5	0.08581984179901525
1111	6	-0.11535293752081799
1111	7	-0.18453466001707852
1111	8	-0.3147805880287038
1111	9	-0.2503541020969706
1111	10	0.09731859673426158
1111	11	-0.15581556294859666
1111	12	-0.058691259423177655
1111	13	-0.14592639911272443
1111	14	-0.9932849746746015
1111	15	-0.5988149552677768
1111	16	-0.02863241793645699
1111	17	0.6314365316575916
1111	18	1.0914444824400178
1111	19	0.33634482943307376
1111	20	0.13419614386800305
1111	21	-0.013191564678905365
1111	22	0.25874527313598605
1111	23	0.21598272690661702
1111	24	0.2162060034492086
1111	25	-0.06862066532508493
1112	1	0.18061533229183624
1112	2	0.47734675444000274
1112	3	0.2711483417707896
1112	4	0.6509811597980573
1112	5	0.4810082557112523
1112	6	-0.11456151308492224
1112	7	-0.10089390632483486
1112	8	-0.01215132051260781
1112	9	-0.04215732378913373
1112	10	0.37762878021663226
1112	11	0.048836274345518405
1112	12	0.025667723381410212
1112	13	-0.0641068365645765
1112	14	0.47097466377709196
1112	15	0.13280935875479827
1112	16	0.5569306279405453
1112	17	0.7289468842211662
1112	18	0.6892069013990749
1112	19	0.29029399162110536
1112	20	0.18950984987532848
1112	21	0.2182502185055668
1112	22	0.24114820241283752
1112	23	1.431889717608982
1112	24	0.6830279356959537
1112	25	0.741014767067135
1113	1	0.2628531699756991
1113	2	0.8199015974684123
1113	3	0.6269986802112939
1113	4	0.6975536708093912
1113	5	0.6221981206555043
1113	6	0.3869980549361003
1113	7	0.23494642666786802
1113	8	0.23242579798963448
1113	9	0.26504909109156927
1113	10	0.6632602806421346
1113	11	0.3600867060752506
1113	12	0.44321446591381886
1113	13	0.27068399260575404
1113	14	1.0690672195102877
1113	15	0.5143080039245902
1113	16	0.8575809820479421
1113	17	1.2597492428007442
1113	18	1.8803394397562556
1113	19	0.6970439301997615
1113	20	0.6242590193519462
1113	21	0.6889727115355768
1113	22	0.9743285461587163
1113	23	0.5002774874623199
1113	24	0.8278496713740964
1113	25	1.2751080634171075
1114	1	-0.29339072155056556
1114	2	-3.290493249170087
1114	3	-1.1487215561574011
1114	4	0.09307697296203798
1114	5	0.1959157558457605
1114	6	0.09286329551958517
1114	7	0.057743059256090135
1114	8	-0.038766618614012316
1114	9	0.14648026661497227
1114	10	0.056718291344566296
1114	11	-0.11675532039416225
1114	12	-0.07337846667244463
1114	13	-0.10479671078419983
1114	14	0.6236970704571476
1114	15	0.11127335245102898
1114	16	0.42533028220504576
1114	17	0.7309172467748155
1114	18	0.9661944899807722
1114	19	0.2598269189350759
1114	20	0.21725199923236715
1114	21	0.15485669965759996
1114	22	0.381316002150065
1114	23	1.95393729789442
1114	24	0.8313848242847754
1114	25	0.6146269219608058
1115	1	-0.0556619331207564
1115	2	0.18026091382224507
1115	3	-0.03668992647758884
1115	4	-0.033088052113917854
1115	5	3.97132809894174E-4
1115	6	-0.43517335454155415
1115	7	-0.6903001457240521
1115	8	-0.46333175642499924
1115	9	-0.4165062375173818
1115	10	-0.4143624199879028
1115	11	-0.23801892674133285
1115	12	-0.49292322315641
1115	13	-0.6471475953589376
1115	14	0.3030900737797211
1115	15	-0.39308495012036104
1115	16	0.05413703544827797
1115	17	0.4879856410871284
1115	18	1.002232971586885
1115	19	0.22167864762530343
1115	20	-0.37682366337533324
1115	21	-0.3379199153295289
1115	22	0.051904974796251224
1115	23	1.812434081712638
1115	24	0.534186313632631
1115	25	0.35660942168435383
1116	1	0.34203510881259547
1116	2	0.3073187246258975
1116	3	0.3422874405141947
1116	4	1.149462614564392
1116	5	0.6425403413065851
1116	6	0.4484169868454728
1116	7	0.2647496404906491
1116	8	0.36429129745937416
1116	9	0.4598832213686066
1116	10	0.9450806613145417
1116	11	0.4490963252167859
1116	12	0.5527895739818689
1116	13	0.49540273659766854
1116	14	1.161754563300022
1116	15	0.609131125835269
1116	16	1.0197753060722263
1116	17	1.322641125138368
1116	18	1.8911322902099243
1116	19	1.0856826759282043
1116	20	0.9228090159409064
1116	21	0.7184115639366411
1116	22	0.9169641041123384
1116	23	2.0390610474749735
1116	24	1.2578953793516021
1116	25	1.4205099773223822
1201	1	0.814293622185442
1201	2	1.1184923273280845
1201	3	0.7894572618555173
1201	4	1.429394847479223
1201	5	1.219813937635692
1201	6	0.7475534344906194
1201	7	0.7442283225376869
1201	8	0.7275223428569504
1201	9	0.9379970020921604
1201	10	1.140160643469919
1201	11	0.7241797063482238
1201	12	0.9566835259288453
1201	13	0.8263913075406748
1201	14	1.7112733957517392
1201	15	1.2141302760912538
1201	16	1.5207020581381698
1201	17	1.9434694131166097
1201	18	2.5615750068415366
1201	19	1.6194175133084414
1201	20	1.3446325665962675
1201	21	1.1773636171306165
1201	22	1.3391487959739194
1201	23	3.103705323386855
1201	24	1.9640963862982588
1201	25	2.2006546462408707
1202	1	0.9632268815057543
1202	2	1.3281448586294218
1202	3	1.0567423857992893
1202	4	1.6484285290101752
1202	5	1.4143207137519127
1202	6	0.9786428276399306
1202	7	0.8997689259680755
1202	8	0.9033312995537273
1202	9	1.0709440098915621
1202	10	1.5238491832266874
1202	11	1.20890050344601
1202	12	1.2713420287351695
1202	13	1.21927876850161
1202	14	1.973795333322883
1202	15	1.4344732030819003
1202	16	1.7261582755121623
1202	17	1.9523363971421333
1202	18	2.702315249178106
1202	19	1.9282643119649947
1202	20	1.556369237243942
1202	21	1.3755323556011305
1202	22	1.913196018523216
1202	23	3.445522118735248
1202	24	2.1241641194252523
1202	25	2.3555179184013184
1203	1	0.5385657092684255
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2211	13	-0.3981362561758104
2211	14	0.10465542695750685
2211	15	-0.17224615949216826
2211	16	-0.2871992488797801
2211	17	0.2524949923690514
2211	18	-5.289544375014643
2211	19	-1.840526684901164
2211	20	-0.9010929853730048
2211	21	-0.7570126537242743
2211	22	-0.5506303133594983
2211	23	-4.762996348092976
2211	24	-2.300338208752681
2211	25	-1.5962031570011952
2212	1	0.3074042110873876
2212	2	0.6291743519441333
2212	3	0.4543604309429554
2212	4	0.9837070313412966
2212	5	0.7421175467121301
2212	6	0.29453142670748633
2212	7	0.1658500736605717
2212	8	-0.002722942677578999
2212	9	0.01382843963955338
2212	10	-3.4551994725271804
2212	11	-1.0240088806037733
2212	12	-0.42833705688991586
2212	13	-0.19065930786060648
2212	14	0.6079811863903117
2212	15	0.13107818572684948
2212	16	0.5230759571240284
2212	17	1.0693048088983872
2212	18	1.6093383960901448
2212	19	0.8751121626088789
2212	20	0.5647499803275906
2212	21	0.47252602973696867
2212	22	0.5205390528841605
2212	23	2.0191501455996317
2212	24	0.9167902772843135
2212	25	1.0932656642987482
2213	1	-1.6172062176907431
2213	2	-3.9477782223158577
2213	3	-1.8082707984035729
2213	4	-3.630630873355763
2213	5	-1.279387968898476
2213	6	-1.130363016236199
2213	7	-1.1213636456113143
2213	8	-1.028168114066716
2213	9	-0.8768973414456553
2213	10	-0.903862293130139
2213	11	-0.9301523752916907
2213	12	-0.8826713720919841
2213	13	-1.3835562708518054
2213	14	-6.313919081156584
2213	15	-2.9432946702899976
2213	16	-2.051069780073135
2213	17	-0.9537119026061305
2213	18	-0.35874192718643627
2213	19	-0.8931984249211453
2213	20	-1.0484921107567686
2213	21	-1.3174482702189962
2213	22	-1.2092131424435735
2213	23	-5.883809059974819
2213	24	-3.3980986333964687
2213	25	-2.668573193279709
2214	1	-0.07010522493909122
2214	2	0.018083146138469175
2214	3	-0.06994121192851566
2214	4	0.21454186896870198
2214	5	-0.08865939838569403
2214	6	-0.08172990108275258
2214	7	-0.2462111739006474
2214	8	-0.3226247533006301
2214	9	-0.20646038482299645
2214	10	0.18061731357131805
2214	11	-0.3602236208623282
2214	12	-0.38134792427499775
2214	13	-0.3115639812008695
2214	14	0.13953079162707382
2214	15	-0.15845010411871385
2214	16	0.13536239294992924
2214	17	0.5085217554549217
2214	18	1.0294184427688435
2214	19	0.17371316911836487
2214	20	0.0011383981749020222
2214	21	-0.04509654743787195
2214	22	0.4990818933560561
2214	23	1.8323674203657845
2214	24	0.9247014965830402
2214	25	0.7196755981098093
2215	1	-2.5047530894741286
2215	2	-4.287660589768748
2215	3	-2.3815010794174647
2215	4	-4.152064018521365
2215	5	-5.00639011551775
2215	6	-2.5215247039445607
2215	7	-1.9922313824582716
2215	8	-2.2417552584315956
2215	9	-2.1296321866040593
2215	10	-3.5766613558418214
2215	11	-2.5071673410042976
2215	12	-2.001993345196727
2215	13	-1.924876902687867
2215	14	-1.2634733177090638
2215	15	-1.8782734503753957
2215	16	-2.733499791822915
2215	17	-7.350836889397623
2215	18	-3.46673089560937
2215	19	-2.596576392835942
2215	20	-2.369541146209457
2215	21	-2.3046472793809585
2215	22	-2.3430179545922876
2215	23	-6.235768318412369
2215	24	-3.902980203076556
2215	25	-3.6419090581559423
2216	1	-0.9041423491596774
2216	2	-0.41418102401720347
2216	3	-0.8261696115379848
2216	4	-2.8191071220647004
2216	5	-1.213815580065571
2216	6	-1.0691886469573397
2216	7	-1.0200888520495859
2216	8	-0.924452812694593
2216	9	-1.0814862903173577
2216	10	-0.7017543718730863
2216	11	-1.2512564265022377
2216	12	-2.0077661377841913
2216	13	-1.5149721260080966
2216	14	-6.923932578303383
2216	15	-3.6283212654635193
2216	16	-2.0053358874044207
2216	17	-0.9394282664075249
2216	18	-0.32728194596331406
2216	19	-0.8429490052660427
2216	20	-1.25708157990276
2216	21	-1.5548112575658735
2216	22	-0.9820032024493557
2216	23	-0.10942697685978686
2216	24	-0.557182626493514
2216	25	-0.5184465081117011
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	48820.0
3	1.0
4	452.0
5	2378.0
6	2952.0
7	2604.0
8	2480.0
9	2301.0
10	2095.0
11	2106.0
12	2070.0
13	1953.0
14	1991.0
15	2033.0
16	2052.0
17	2274.0
18	2431.0
19	2985.0
20	3704.0
21	4548.0
22	5647.0
23	6914.0
24	8106.0
25	9515.0
26	10669.0
27	12438.0
28	13633.0
29	16375.0
30	19583.0
31	21970.0
32	28315.0
33	37047.0
34	48050.0
35	63042.0
36	89263.0
37	124804.0
38	311555.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.194780299672054	17.55206467008736	12.092204104616195	25.160950925624388
2	13.69542147168148	18.872048283255964	43.40420900249797	24.028321242564584
3	20.51146987186045	23.301176916161864	28.104723118882248	28.08263009309544
4	10.722937720789412	15.798672706021144	38.79657663501157	34.68181293817788
5	12.261615967036805	37.99854817417121	36.19007817439976	13.549757684392219
6	33.26046340054125	35.97845023172203	17.605433797357747	13.15565257037897
7	29.362580061067305	30.59219618144346	20.53252171415016	19.512702043339072
8	25.438218484615017	38.10063246233737	19.171596216543264	17.289552836504352
9	26.33977688613488	15.832900752310092	19.37995631747856	38.44736604407647
10	14.270166731966391	29.740760088807626	34.26048060598145	21.728592573244526
11	35.380312955668245	22.715293042286014	22.62312563929574	19.281268362750005
12	23.241227492684814	25.06270545899308	31.34435063260423	20.351716415717878
13	29.427689736369057	21.19961436847397	25.08889981850074	24.283796076656238
14	20.360405456138157	22.161888646466405	28.103137769120696	29.374568128274742
15	24.166257689407253	29.947146717100548	22.67663736902299	23.20995822446921
16	21.803891099699772	28.519242760093107	28.300954656794737	21.375911483412388
17	22.020985322027407	28.419201504361602	27.971070638831335	21.588742534779655
18	21.322585278589056	27.401445938358282	30.149468763465897	21.126500019586764
19	23.281095258306397	27.265554430985883	27.852688628424044	21.600661682283675
20	23.012937493266822	27.354925203246722	28.598182504730907	21.03395479875555
21	22.7036432867607	26.85328368100483	27.897440808424644	22.545632223809832
22	22.485751257274014	27.780831101384006	27.56904532063344	22.164372320708537
23	21.078411634692085	27.236379742454897	29.42920230230045	22.25600632055257
24	22.23881376740298	27.79144282592228	28.23506107913732	21.73468232753742
25	22.281895409046093	27.094637683998375	27.96524952823721	22.65821737871833
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	88.0
1	88.0
2	154.0
3	220.0
4	220.0
5	220.0
6	528.0
7	836.0
8	836.0
9	836.0
10	1266.5
11	1697.0
12	1697.0
13	1697.0
14	2865.5
15	4034.0
16	4034.0
17	4034.0
18	6854.0
19	9674.0
20	9674.0
21	9674.0
22	15243.5
23	20813.0
24	20813.0
25	20813.0
26	29636.0
27	38459.0
28	38459.0
29	38459.0
30	50101.0
31	61743.0
32	61743.0
33	61743.0
34	72033.5
35	82324.0
36	82324.0
37	82324.0
38	91102.0
39	99880.0
40	99880.0
41	99880.0
42	107875.0
43	115870.0
44	115870.0
45	115870.0
46	125454.5
47	135039.0
48	135039.0
49	135039.0
50	128021.5
51	121004.0
52	121004.0
53	121004.0
54	105695.0
55	90386.0
56	90386.0
57	90386.0
58	76622.5
59	62859.0
60	62859.0
61	62859.0
62	50769.0
63	38679.0
64	38679.0
65	38679.0
66	29566.5
67	20454.0
68	20454.0
69	20454.0
70	14865.0
71	9276.0
72	9276.0
73	9276.0
74	6497.5
75	3719.0
76	3719.0
77	3719.0
78	2552.0
79	1385.0
80	1385.0
81	1385.0
82	926.5
83	468.0
84	468.0
85	468.0
86	321.0
87	174.0
88	174.0
89	174.0
90	111.5
91	49.0
92	49.0
93	49.0
94	32.0
95	15.0
96	15.0
97	15.0
98	13.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11086257392651519
2	0.0447149341352284
3	0.03416177449747377
4	0.03198586529381302
5	0.03405297903729073
6	0.01816884185056726
7	0.01849522823111637
8	0.022629455718071796
9	0.027525251426308482
10	0.03437936541783985
11	0.019148000992214597
12	0.019256796452397636
13	0.015666546266357398
14	0.02077993289496016
15	0.021432705656058385
16	0.02034475105422801
17	0.024805364921732544
18	0.0182776373107503
19	0.031115501612348717
20	0.02165029657642446
21	0.025349342222647734
22	0.03296502443546036
23	0.027090069585576332
24	0.037860820143697044
25	0.02850441056795582
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	919156.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.8240872953257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.71157648575652	77.71463280629321
2	5.4945006024253225	9.211429962838396
3	0.9267409333910008	2.3304963870215665
4	0.2950671412539373	0.9893493522580895
5	0.1551886610905412	0.650427393724912
6	0.09095374100973692	0.457446859574198
7	0.059049479986554264	0.3464838135595558
8	0.045700419897405566	0.3064636789530531
9	0.03224703282999443	0.2432770285461018
>10	0.16320712398190557	2.4610361064730033
>50	0.01242959773461943	0.6912183503874912
>100	0.010331016744242126	1.747322598685997
>500	0.0018308128075876574	1.1093414796848833
>1k	0.0011769510905920656	1.7410741819995061
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	4028	0.43822811361727504	No Hit
CCCATGTACTCTGCGTTGATACCAC	2905	0.3160508118317239	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1723	0.18745457789537356	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1679	0.18266757764731995	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1196	0.13011937037891283	No Hit
GTATCAACGCAGAGTACATGGGAAG	1131	0.12304766546701539	No Hit
GTCCTACAGTGGACATTTCTAAATT	1103	0.12000139258189035	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1073	0.11673752877639922	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1045	0.11369125589127417	No Hit
CATGTACTCTGCGTTGATACCACTG	969	0.10542280091736331	No Hit
TATCAACGCAGAGTACTTTTTTTTT	947	0.1030293007933365	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.0879546018303749E-4	0.0
10	0.0	0.0	0.0	1.0879546018303749E-4	0.0
11	0.0	0.0	0.0	1.0879546018303749E-4	0.0
12	0.0	0.0	0.0	3.263863805491124E-4	0.0
13	0.0	0.0	0.0	3.263863805491124E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGACCG	40	2.7605405E-4	16.624239	5
GCGTTTA	65	1.9129584E-7	16.075312	13
GCCGGTT	65	3.3701017E-6	14.614716	11
AAACCGA	40	0.0052785343	14.249348	6
GTACTAG	75	9.553223E-7	13.947114	1
GTCCTAC	730	0.0	13.677899	1
GTCTTAC	105	1.3478711E-9	13.584852	1
TGCGTTT	100	1.0057192E-8	13.29939	12
CCGGTTT	75	1.4777226E-5	12.666087	12
TGTAGAA	370	0.0	12.580504	2
TAGGACC	370	0.0	12.580504	4
TAGGACT	145	7.2759576E-12	12.447706	4
TCCAACG	115	5.30963E-9	12.391412	18
CCTAGAC	100	1.4397483E-7	12.350106	3
CTACACT	200	0.0	12.349433	4
GTAGGAC	635	0.0	12.267822	3
TGTAGGA	690	0.0	12.253062	2
TCCTACA	855	0.0	12.110556	2
GTGCATT	560	0.0	12.04409	11
GTGCTAT	120	9.844371E-9	11.8867445	1
>>END_MODULE