FastQCFastQC Report
Thu 26 May 2016
SRR1513055_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513055_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919156
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC46950.510794685559361No Hit
CCCATGTACTCTGCGTTGATACCAC37340.40624224832346195No Hit
GTATCAACGCAGAGTACTTTTTTTT24460.2661136956077097No Hit
GAGTACATGGGAAGCAGTGGTATCA18000.19583182832946747No Hit
TATCAACGCAGAGTACTTTTTTTTT17470.19006566893976648No Hit
GGTATCAACGCAGAGTACTTTTTTT17240.18756337335555662No Hit
GCTTCCCATGTACTCTGCGTTGATA15310.16656584954023038No Hit
GTATCAACGCAGAGTACATGGGAAG11670.12696430203360476No Hit
CATGTACTCTGCGTTGATACCACTG11650.12674671111323868No Hit
GTCCTACAGTGTGCATTTCTCATTT11220.12206850632536805No Hit
GTCCTACAGTGGACATTTCTAAATT11150.1213069381040868No Hit
ACGCAGAGTACATGGGAAGCAGTGG10130.11020980116541697No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10130.11020980116541697No Hit
CACACACACACACACACACACACAC10120.11010100570523393No Hit
GCGTTGATACCACTGCTTCCCATGT9900.10770750558120712No Hit
CTGTAGGACGTGGAATATGGCAAGA9540.10379086901461776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG551.9202745E-413.8505525
TAGGACC3950.013.7409754
GTTCTAG1204.9112714E-1113.4979691
TCCAACG1351.8189894E-1213.35574118
TGTAGGA7200.013.0995382
CCTACAC1403.6379788E-1212.9230593
ATAGGAC1051.9254003E-812.6963393
TCTAGGC1001.3966746E-712.378933
GTCCTAC6700.012.372151
GTAGGAC6700.012.3647183
CGTTTCC701.1000416E-412.20224914
CGGGACA550.003013515812.1192324
ACCGTAT550.003095836412.07570658
ACAGGTC1353.783498E-1011.9479228
GGACAGT1603.6379788E-1211.9028186
TCTGGAC1603.6379788E-1211.9028183
GTGCAGG1700.011.76985551
AGGACCT4900.011.6599035
TAGGACG4250.011.6507594
TTAGACA907.2595194E-611.638314
Produced by FastQC (version 0.11.5)