FastQCFastQC Report
Thu 26 May 2016
SRR1513054_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513054_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1787293
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC198751.11201688810956No Hit
CCCATGTACTCTGCGTTGATACCAC156180.8738354595469239No Hit
GAGTACATGGGAAGCAGTGGTATCA79880.4469328755833542No Hit
GTATCAACGCAGAGTACATGGGAAG54320.30392330748232105No Hit
GCGTTGATACCACTGCTTCCCATGT54040.30235669249529873No Hit
CATGTACTCTGCGTTGATACCACTG51150.28618698780781887No Hit
GCTTCCCATGTACTCTGCGTTGATA43130.2413146585366809No Hit
ACGCAGAGTACATGGGAAGCAGTGG42890.2399718456906618No Hit
TATCAACGCAGAGTACATGGGAAGC40560.22693537097722644No Hit
ACTCTGCGTTGATACCACTGCTTCC35360.19784109264681282No Hit
GGTATCAACGCAGAGTACATGGGAA25740.14401667773554758No Hit
CAGTGGTATCAACGCAGAGTACATG20700.11581760796914664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCAAT250.0060368418.99759113
GCGTTTA3000.013.93156813
TAGGACC4650.013.8942144
GTATCGA802.0014668E-613.06157618
GTAGGAC10700.012.6083493
TGCGTTT3550.012.30795512
CGTGAAA4300.012.1489439
AGGACGT7700.012.0920855
TGTAGGA12500.012.0095042
CCAACGA951.0409385E-611.99915219
GGACGTG7600.011.9988166
GGCGAGG3050.011.83522819
TAGGACG8250.011.6317614
GTCCTAA4800.011.4944431
TGGCGAG8700.011.46470618
TTAGGAC5200.011.3277083
AGGACCT7400.011.2983965
CGTTTAT3700.011.29586514
GACGTGA4150.011.2148187
GTATAGG2550.011.1913441
Produced by FastQC (version 0.11.5)