##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513054_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1787293
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.699026964241455	31.0	28.0	34.0	20.0	34.0
2	28.436006295554225	31.0	28.0	34.0	16.0	34.0
3	28.75564051333497	31.0	28.0	34.0	16.0	34.0
4	31.545827125155192	35.0	32.0	37.0	16.0	37.0
5	31.794622370254906	35.0	32.0	37.0	17.0	37.0
6	32.230219107891095	35.0	32.0	37.0	22.0	37.0
7	32.227008106673054	35.0	33.0	37.0	22.0	37.0
8	32.20018038452565	35.0	33.0	37.0	22.0	37.0
9	33.482219199649975	38.0	33.0	39.0	19.0	39.0
10	32.95312520107223	38.0	33.0	39.0	16.0	39.0
11	33.35856739773501	38.0	33.0	39.0	18.0	39.0
12	33.207456751635014	38.0	33.0	39.0	17.0	39.0
13	33.28016503169878	38.0	33.0	39.0	17.0	39.0
14	33.52005239208121	39.0	32.0	41.0	10.0	41.0
15	34.043215074417006	39.0	33.0	41.0	17.0	41.0
16	33.579499276279826	39.0	33.0	41.0	10.0	41.0
17	33.10856585909529	38.0	32.0	41.0	10.0	41.0
18	32.47997838071318	38.0	31.0	41.0	8.0	41.0
19	33.37064823730636	39.0	32.0	41.0	10.0	41.0
20	33.66538278838445	39.0	33.0	41.0	8.0	41.0
21	33.6877825851721	39.0	33.0	41.0	2.0	41.0
22	33.24929600239021	39.0	33.0	41.0	2.0	41.0
23	31.528798579751612	38.0	30.0	40.0	2.0	41.0
24	32.597789506253314	38.0	32.0	41.0	2.0	41.0
25	32.31395132191532	38.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9923041768241028
1101	2	1.3145735927124385
1101	3	1.125723457715221
1101	4	1.3079337099931472
1101	5	1.424639813243136
1101	6	1.091591598863836
1101	7	1.155053867071416
1101	8	1.0615475447767864
1101	9	1.2388585404895593
1101	10	1.6035548721891075
1101	11	1.1689380333208277
1101	12	1.3317836799252234
1101	13	1.1707069781228938
1101	14	0.22686381447691417
1101	15	1.0039980094834604
1101	16	1.6072274829135509
1101	17	2.293533762194521
1101	18	-3.817166515345196
1101	19	-0.19474200618537196
1101	20	0.7086006846384905
1101	21	1.1860106223886007
1101	22	1.7531222177260872
1101	23	3.4511293496599613
1101	24	2.4294266965089832
1101	25	2.7231091373207903
1102	1	1.3720658786673212
1102	2	1.7047168055598156
1102	3	1.4257371606947835
1102	4	2.002577181860257
1102	5	1.859202069889914
1102	6	1.4263306573688723
1102	7	1.3852077749272382
1102	8	1.3478388834910433
1102	9	1.5467177005971848
1102	10	1.9267834278616363
1102	11	1.5918146563719162
1102	12	1.6519374206715298
1102	13	1.5635573649814063
1102	14	2.481024005650191
1102	15	2.050817414239397
1102	16	2.3307875838476946
1102	17	1.9182011307211226
1102	18	2.310239109560804
1102	19	2.0990639128333655
1102	20	1.9542182378210882
1102	21	2.041433492995708
1102	22	2.3736298964079268
1102	23	3.889195558446577
1102	24	2.7421968708042
1102	25	3.1713643469596704
1103	1	0.7586260846680375
1103	2	1.0843793580542709
1103	3	0.8722950748027323
1103	4	1.3819377437784404
1103	5	1.1934897293955409
1103	6	0.7540047232540488
1103	7	0.7859299907830888
1103	8	0.8219867375379195
1103	9	1.0107188060983958
1103	10	1.2319227951644152
1103	11	0.958390501170328
1103	12	1.1227395157311548
1103	13	0.9233869560133598
1103	14	1.666163777404634
1103	15	1.2257725538089232
1103	16	1.5290704168967864
1103	17	2.009349840857354
1103	18	2.084066907523713
1103	19	1.522070741325635
1103	20	1.2881025241998358
1103	21	1.3067238555866254
1103	22	1.64322726477576
1103	23	2.996202818353961
1103	24	1.9850268191017904
1103	25	2.157557984934982
1104	1	0.5978665114012394
1104	2	0.9757557361801155
1104	3	0.6943796198458365
1104	4	1.2804521459844445
1104	5	1.1051403997758733
1104	6	0.8851568336832472
1104	7	0.6876303079082433
1104	8	0.7216485816723477
1104	9	0.8957987848880293
1104	10	1.3293959129752366
1104	11	0.8860865999998708
1104	12	0.9503546621759753
1104	13	0.91312655829514
1104	14	1.7499649515841043
1104	15	1.1093381100487747
1104	16	1.456324104602885
1104	17	1.8937518698453886
1104	18	2.6011274112409453
1104	19	1.6198865329060084
1104	20	0.9642087039262819
1104	21	1.2469062785094707
1104	22	1.4842296740603445
1104	23	3.0971146951837447
1104	24	2.1156770723025673
1104	25	2.0651113655557154
1105	1	0.6052880599979922
1105	2	1.0403688651797012
1105	3	0.674716025603697
1105	4	0.732817974689052
1105	5	1.0651025456923442
1105	6	0.8141971698159693
1105	7	0.6418561280199526
1105	8	0.4741234185978982
1105	9	0.8575178898666564
1105	10	1.247761583010032
1105	11	0.8525712085130266
1105	12	0.8137018327532317
1105	13	0.7949850351267713
1105	14	1.5047010909003191
1105	15	0.8502122776762135
1105	16	1.2484531289174825
1105	17	1.716057522508926
1105	18	1.780622248805301
1105	19	1.3595167137586799
1105	20	0.8980084904432601
1105	21	0.9084267371270442
1105	22	1.4956988483282245
1105	23	2.8163276001910127
1105	24	1.9094761221382868
1105	25	1.9426061649981037
1106	1	-0.6222211987726176
1106	2	-0.23008812849049676
1106	3	-0.4539243605380001
1106	4	-2.6733895666429923
1106	5	-0.6974326241837225
1106	6	-0.6059153427437742
1106	7	-0.6389523729749982
1106	8	-0.5167018873632045
1106	9	-0.4587577302045176
1106	10	-0.4040137002374635
1106	11	-0.42781406396232313
1106	12	-0.43040582250903014
1106	13	-0.4599252952708639
1106	14	0.2378772398519189
1106	15	-0.31875368916294633
1106	16	-1.344900191356814
1106	17	-5.727723838051762
1106	18	-7.504351922872328
1106	19	-3.339323210660364
1106	20	-1.8792539195172004
1106	21	-1.3811373685414239
1106	22	-1.578354889276305
1106	23	-5.173166967686463
1106	24	-2.3095976696684666
1106	25	-0.9062135551743751
1107	1	0.4183271937056645
1107	2	0.8181711544282315
1107	3	0.5996105481107534
1107	4	0.8513962345666961
1107	5	0.9486084587636121
1107	6	0.4256256737642161
1107	7	0.35989504062187194
1107	8	0.4290457351027399
1107	9	0.3529392420489188
1107	10	0.7813070941110496
1107	11	0.4646954418037126
1107	12	0.5800402913848473
1107	13	0.5228144098296639
1107	14	1.2628832599276194
1107	15	0.7002136344614982
1107	16	-5.657457454912247
1107	17	-0.961309615548501
1107	18	0.7749570421066068
1107	19	0.7455624535031404
1107	20	0.5147278288546602
1107	21	0.6085834709529081
1107	22	0.607074462776076
1107	23	2.496176273405755
1107	24	1.6943922376318667
1107	25	1.3945837549253284
1108	1	0.5694151578285265
1108	2	0.8836322708284925
1108	3	0.7145707124641127
1108	4	1.3449035252416515
1108	5	0.8941037736907056
1108	6	0.5451805918380757
1108	7	0.4826553502876578
1108	8	0.5339955021017886
1108	9	0.6991194043979689
1108	10	1.0611641618065946
1108	11	0.7280695711818765
1108	12	0.7351222175260546
1108	13	0.6905560543654161
1108	14	1.1871806242609395
1108	15	0.8259255400585346
1108	16	1.0160923020652533
1108	17	1.062247657588749
1108	18	0.9852456626407502
1108	19	0.9688443846551209
1108	20	0.7249947777948762
1108	21	0.892501645027167
1108	22	1.181093854468216
1108	23	2.828894116768989
1108	24	1.6459190202144214
1108	25	1.8129796811667447
1109	1	0.36399568637502355
1109	2	0.6709988876322832
1109	3	0.4764305977794976
1109	4	0.5182580502977423
1109	5	0.326876149656794
1109	6	0.1729236733638757
1109	7	0.2698823510355979
1109	8	0.22773833431320867
1109	9	0.37585774895045176
1109	10	0.8444669541277108
1109	11	0.3912651583172533
1109	12	0.4925118959104964
1109	13	0.3420494475342011
1109	14	-0.7890241875066977
1109	15	0.04954870136494094
1109	16	-0.03403173184150177
1109	17	0.9021510482643862
1109	18	1.7751259917357416
1109	19	1.0175697648595587
1109	20	0.8423364631601231
1109	21	0.7653600344562363
1109	22	0.6067694964527561
1109	23	-4.05266908442772
1109	24	-1.4008673173572568
1109	25	0.001303665210627969
1110	1	0.31195468843366925
1110	2	0.6866941523858507
1110	3	0.4583934960154039
1110	4	1.0252389138349578
1110	5	0.6847283075279087
1110	6	0.3155911880292166
1110	7	0.19024908008726982
1110	8	0.24326667210825548
1110	9	0.26006851185159974
1110	10	0.6118716104665296
1110	11	0.36240538242945064
1110	12	0.3593920201216463
1110	13	0.21299352639564262
1110	14	1.1832321632197207
1110	15	0.5982442959632621
1110	16	0.9237300376328577
1110	17	1.2654798439544166
1110	18	1.9669079370179574
1110	19	1.1302927213862617
1110	20	0.7354819489947175
1110	21	0.5765519160231349
1110	22	0.8818190794694587
1110	23	2.165617800325254
1110	24	1.3107661188171775
1110	25	1.7404530788098285
1111	1	0.5198481889229924
1111	2	0.8576643262170514
1111	3	0.7421299693507244
1111	4	1.2302667046631264
1111	5	0.8464395545018242
1111	6	0.4291347878662606
1111	7	0.3253869572457191
1111	8	0.14378003371367498
1111	9	0.4088637743777639
1111	10	0.62696185066482
1111	11	0.45468872557008666
1111	12	0.6383632365966676
1111	13	0.42059202736102463
1111	14	-0.17890238465839303
1111	15	0.2339089579858964
1111	16	0.6349957416749703
1111	17	1.2254816545335032
1111	18	1.9825959804843407
1111	19	0.9529355737350969
1111	20	0.7216154105253736
1111	21	0.6468211147950669
1111	22	0.9659189172977634
1111	23	0.6012072296650608
1111	24	0.7511582652742916
1111	25	0.3861602113988454
1112	1	0.4044355131209052
1112	2	0.797975350029084
1112	3	0.47718112450457895
1112	4	0.9874584228483592
1112	5	0.6628553157583106
1112	6	0.20653822815685174
1112	7	0.16031449166441547
1112	8	0.00918856896583975
1112	9	0.2090237962065018
1112	10	0.5714361825319827
1112	11	0.27203268492478117
1112	12	0.22292631325760937
1112	13	0.17592985155268082
1112	14	0.7179177325757706
1112	15	0.3202772548377055
1112	16	0.6605056995486009
1112	17	0.939945042242087
1112	18	0.8554233088287617
1112	19	0.41487510264179406
1112	20	0.3014128631637405
1112	21	0.38196747280905186
1112	22	0.4453067831630051
1112	23	2.004159352396922
1112	24	1.1733850947271236
1112	25	1.145659794662926
1113	1	0.05497175516913444
1113	2	0.5681302251414841
1113	3	0.2768141492182892
1113	4	0.21557348773738738
1113	5	0.4934048306289931
1113	6	0.060926415227093855
1113	7	0.04274825857268638
1113	8	0.029719163881537725
1113	9	0.18368895775630278
1113	10	0.44927616317038144
1113	11	0.09795791076592053
1113	12	0.15519381657139775
1113	13	0.02646468034564009
1113	14	0.6929008765623408
1113	15	0.2765929454455076
1113	16	0.6523035192490241
1113	17	1.1001295191639286
1113	18	1.7713452194085662
1113	19	0.8611602728242289
1113	20	0.567211791091843
1113	21	0.3701030770080749
1113	22	0.7207648398074582
1113	23	0.5229104822650719
1113	24	0.5703036093722815
1113	25	1.0178049454013163
1114	1	-0.5037930061364051
1114	2	-3.02591533189446
1114	3	-1.2763406935792752
1114	4	-0.012042211572104833
1114	5	0.23130197144769582
1114	6	-0.050681228035571735
1114	7	-0.07485934191078769
1114	8	-0.04686435881206563
1114	9	0.11383988532259792
1114	10	0.13805565992109337
1114	11	0.012985458345788459
1114	12	-0.035581049039400625
1114	13	-0.09334815250073802
1114	14	0.5979834304708405
1114	15	0.11678074844287778
1114	16	0.5037544059880688
1114	17	0.6608015779570309
1114	18	0.8947461278937041
1114	19	0.31840790392638496
1114	20	0.2945289126692572
1114	21	-0.09079259073364909
1114	22	0.1765032770648176
1114	23	1.7584033973410094
1114	24	0.7501092352102603
1114	25	0.8604897208356803
1115	1	-0.5628689422521838
1115	2	-0.25917644587852706
1115	3	-0.424166172120227
1115	4	-0.45955571608200074
1115	5	-0.4913885588355278
1115	6	-0.7738085122781477
1115	7	-1.0349838748939426
1115	8	-0.9124552646455868
1115	9	-0.9597768829305195
1115	10	-0.8353471376284389
1115	11	-0.9959538347906829
1115	12	-1.0383866606014678
1115	13	-1.1855960844562148
1115	14	-0.2768266396615786
1115	15	-1.0339735721287155
1115	16	-0.521692201566708
1115	17	0.03444095863797969
1115	18	0.48674082002573726
1115	19	-0.3486092069510178
1115	20	-0.8679491285812304
1115	21	-0.7497481531972099
1115	22	-0.521769341657695
1115	23	1.1679982329917529
1115	24	0.0038266069779382406
1115	25	-0.5378919895354315
1116	1	-0.0398149413405946
1116	2	-0.15163240352154617
1116	3	-0.15001786968544195
1116	4	0.5790833697873445
1116	5	-0.07152206388635207
1116	6	-0.09315296484913915
1116	7	-0.2165492865389318
1116	8	-0.2125329085119887
1116	9	-0.25094827182824275
1116	10	0.26741650702235376
1116	11	-0.21742024388111503
1116	12	-0.1914149038891182
1116	13	-0.3358336534877111
1116	14	0.19059592846892315
1116	15	-0.24315714554854395
1116	16	0.17788605709071703
1116	17	0.38204194866352736
1116	18	0.8570253463716568
1116	19	0.08799139618017904
1116	20	-0.15810425731635291
1116	21	-0.2833667548656251
1116	22	0.14281591289758921
1116	23	1.3340267889748354
1116	24	0.4144904487858483
1116	25	0.5655038033859938
1201	1	1.364110535979787
1201	2	1.6987373128452994
1201	3	1.430106426749827
1201	4	2.1134959622164367
1201	5	1.7556058745310388
1201	6	1.378983112846477
1201	7	1.3653339217162284
1201	8	1.335234997900109
1201	9	1.6250332699441827
1201	10	1.9074141618065923
1201	11	1.5409687308457407
1201	12	1.6310570914756326
1201	13	1.5845476510040655
1201	14	2.5089348259416084
1201	15	1.9858835232518075
1201	16	2.26444314240139
1201	17	2.732237153387068
1201	18	3.2433023853298266
1201	19	2.4605985863357915
1201	20	2.140635534097399
1201	21	2.0431003845229654
1201	22	2.311892173795947
1201	23	3.953211343659749
1201	24	2.9383875076093773
1201	25	3.003116235788596
1202	1	1.3954117290477086
1202	2	1.6022658624109667
1202	3	1.3694134073287962
1202	4	2.0146111627854886
1202	5	1.7274414693406008
1202	6	1.331214591733378
1202	7	1.1461625219360911
1202	8	1.2807176138119942
1202	9	1.3594642455223962
1202	10	1.885943384965259
1202	11	1.4951641374803586
1202	12	1.5004664043029976
1202	13	1.4707486942649055
1202	14	2.256790240551574
1202	15	1.7271443276835114
1202	16	2.130481795862039
1202	17	2.4333559913583116
1202	18	3.144557289086862
1202	19	2.274256492700978
1202	20	1.9734765607609077
1202	21	1.9253370635478149
1202	22	2.316298010883436
1202	23	3.9801818726188536
1202	24	2.6948129112263004
1202	25	2.916365405922022
1203	1	0.9915683095548502
1203	2	1.1957722243708844
1203	3	0.9384351407187879
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1203	5	1.4849336754962117
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2211	16	-0.3308313363118742
2211	17	0.22263298931128617
2211	18	-4.948346404207129
2211	19	-1.6220986839042268
2211	20	-0.8981580964626446
2211	21	-0.7012963012126434
2211	22	-0.36367056715667445
2211	23	-4.84300259117423
2211	24	-2.197560291171868
2211	25	-1.5775616960606769
2212	1	0.548883885618153
2212	2	0.8422090298903271
2212	3	0.6745849271051938
2212	4	1.2079632267341829
2212	5	1.1327320608264522
2212	6	0.6199265051287668
2212	7	0.5560011214319331
2212	8	0.5953974210783386
2212	9	0.43181840937309346
2212	10	-3.362064338548773
2212	11	-0.7278881402609159
2212	12	0.11787275057509561
2212	13	0.3880542775352751
2212	14	1.2605086271038672
2212	15	0.8828143104920798
2212	16	1.00069962260541
2212	17	1.6114605218389215
2212	18	2.07889882539839
2212	19	1.3804204329848986
2212	20	1.0216028090670903
2212	21	0.966485298189923
2212	22	1.144523136628841
2212	23	2.490550122454845
2212	24	1.6614698375562824
2212	25	1.488315502062271
2213	1	-1.4417650759010705
2213	2	-3.7523827402535126
2213	3	-1.7063621910216824
2213	4	-3.3433042120140435
2213	5	-1.2266397282234607
2213	6	-1.0417109052403042
2213	7	-0.9139238356885677
2213	8	-0.8599632929818277
2213	9	-0.5800126729745898
2213	10	-0.7251228621708421
2213	11	-0.7743065118324353
2213	12	-0.6680069382265188
2213	13	-0.9018437038453442
2213	14	-6.038780991697152
2213	15	-2.762804562701895
2213	16	-2.1014610930586635
2213	17	-0.8007520401747001
2213	18	-0.05798826844931426
2213	19	-0.5789014344059353
2213	20	-0.7854933342341397
2213	21	-0.9661312383095293
2213	22	-0.8140391296139953
2213	23	-5.687718818955371
2213	24	-3.2561724567148573
2213	25	-2.2984896921961386
2214	1	-0.2349258256155089
2214	2	-0.16867094122662607
2214	3	-0.2913933997595386
2214	4	0.06720202826534205
2214	5	-0.2862025611957826
2214	6	-0.39807632300280815
2214	7	-0.6850260713441472
2214	8	-0.6845439546246901
2214	9	-0.49477298184174856
2214	10	-0.1112143947513502
2214	11	-0.5357187206789362
2214	12	-0.4469705751774242
2214	13	-0.4664698205230593
2214	14	0.01523381153168657
2214	15	-0.2767073041964494
2214	16	0.11404436908220816
2214	17	0.3093917539504005
2214	18	0.9707289579713745
2214	19	-0.12562498185623383
2214	20	-0.1901011377399655
2214	21	-0.30657687187577665
2214	22	0.22113003965706923
2214	23	1.5985431700058292
2214	24	0.7132413162390066
2214	25	0.48945410369078957
2215	1	-2.889112679968566
2215	2	-5.014721957602237
2215	3	-3.0529374529547084
2215	4	-4.631154210550523
2215	5	-5.517847887029863
2215	6	-3.062542512528541
2215	7	-2.4658149527121935
2215	8	-2.9919892474082843
2215	9	-2.589558425861817
2215	10	-3.9859313359070896
2215	11	-2.9575692158137556
2215	12	-2.5820583261652175
2215	13	-2.5347924205855925
2215	14	-1.8468167879294022
2215	15	-2.4314068804228484
2215	16	-3.4093522735842114
2215	17	-7.629882009262165
2215	18	-4.0414308862763875
2215	19	-3.2118871112496095
2215	20	-2.840399800367024
2215	21	-2.870068828818372
2215	22	-2.5375667656011274
2215	23	-6.958184421997906
2215	24	-4.454661855118477
2215	25	-4.3611074250088215
2216	1	-1.6687786547283423
2216	2	-1.3118659093352925
2216	3	-1.5369715987278774
2216	4	-4.12203232553178
2216	5	-2.1236396294030193
2216	6	-1.920824867670568
2216	7	-1.9836708553538358
2216	8	-1.720726972875461
2216	9	-1.8383214509250543
2216	10	-1.392147940104234
2216	11	-2.0179859338226755
2216	12	-2.7974437515367327
2216	13	-2.1902696963170243
2216	14	-7.799038666002936
2216	15	-4.35834652545557
2216	16	-2.6240146133123616
2216	17	-1.7419971103821368
2216	18	-0.828889915157248
2216	19	-1.4896519756724516
2216	20	-2.015867369611854
2216	21	-2.325387344963737
2216	22	-1.944472569553625
2216	23	-0.8311718522937959
2216	24	-1.4899681499402675
2216	25	-1.4302459785238817
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	103243.0
3	4.0
4	854.0
5	5188.0
6	6270.0
7	5498.0
8	5090.0
9	4754.0
10	4541.0
11	4408.0
12	4376.0
13	4347.0
14	4293.0
15	4403.0
16	4522.0
17	5044.0
18	5473.0
19	6535.0
20	7967.0
21	10115.0
22	12368.0
23	15178.0
24	17954.0
25	20817.0
26	23476.0
27	26787.0
28	29588.0
29	35398.0
30	41407.0
31	47545.0
32	59868.0
33	77682.0
34	99692.0
35	128718.0
36	178583.0
37	241257.0
38	534050.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.00001960436158	17.76552847477507	11.807650966186957	25.4268009546764
2	13.624318187924873	19.578810310700305	41.62938531674593	25.16748618462889
3	20.25237387602952	24.378734324582247	28.177663596649566	27.191228202738664
4	11.064116297890775	16.215673171315963	38.130498197257324	34.589712333535935
5	12.351551122161016	38.079369006213895	36.18105921611605	13.38802065550905
6	33.25347592385845	35.05124696757297	18.155109167373183	13.5401679411954
7	28.676998118535614	30.893566869214162	21.305765384413057	19.123669627837167
8	26.390926149887317	36.279338657043766	20.108414309211355	17.22132088385756
9	27.248040431063192	15.193606250472227	19.343442964720765	38.21491035374382
10	14.507298146553993	29.557129727787363	34.4589050373387	21.476667088319942
11	34.34064412002218	23.115860234159324	22.607270791979197	19.936224853839295
12	23.939672057753654	25.41949745369075	30.86322681739325	19.777603671162346
13	29.40803842884491	20.99943424449256	24.663694445019587	24.92883288164294
14	20.964883267883653	22.351442663116323	27.67046716910977	29.013206899890253
15	25.726196345695485	28.576305390572053	22.406833367004705	23.29066489672776
16	21.787607261640332	27.7638707706049	28.883930495184597	21.56459147257018
17	23.30817473491687	27.890449843798798	27.845732981568144	20.955642439716186
18	22.704959041209502	26.458740692074283	29.909374688709832	20.92692557800638
19	23.589731247582783	27.48906760816114	27.81447872374365	21.10672242051243
20	23.687547779719125	27.181077720184057	27.65655238336152	21.474822116735297
21	22.4370745434326	26.943451179986983	28.05787244712068	22.561601829459743
22	22.843743125921094	27.647104588988153	25.981429562135517	23.52772272295524
23	21.14816543593773	27.401264645338507	28.72590731978692	22.72466259893684
24	22.52097745333073	28.786702497143956	26.928943372210632	21.763376677314685
25	22.79124018304095	26.517598885993426	26.572336548191533	24.11882438277409
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	152.0
1	152.0
2	268.0
3	384.0
4	384.0
5	384.0
6	750.5
7	1117.0
8	1117.0
9	1117.0
10	1689.5
11	2262.0
12	2262.0
13	2262.0
14	4102.0
15	5942.0
16	5942.0
17	5942.0
18	11044.5
19	16147.0
20	16147.0
21	16147.0
22	26307.5
23	36468.0
24	36468.0
25	36468.0
26	52707.5
27	68947.0
28	68947.0
29	68947.0
30	90292.5
31	111638.0
32	111638.0
33	111638.0
34	130928.0
35	150218.0
36	150218.0
37	150218.0
38	168578.5
39	186939.0
40	186939.0
41	186939.0
42	204930.0
43	222921.0
44	222921.0
45	222921.0
46	259142.5
47	295364.0
48	295364.0
49	295364.0
50	273461.5
51	251559.0
52	251559.0
53	251559.0
54	213494.5
55	175430.0
56	175430.0
57	175430.0
58	146786.5
59	118143.0
60	118143.0
61	118143.0
62	95679.0
63	73215.0
64	73215.0
65	73215.0
66	56633.5
67	40052.0
68	40052.0
69	40052.0
70	29232.0
71	18412.0
72	18412.0
73	18412.0
74	12868.0
75	7324.0
76	7324.0
77	7324.0
78	5165.0
79	3006.0
80	3006.0
81	3006.0
82	2072.5
83	1139.0
84	1139.0
85	1139.0
86	760.0
87	381.0
88	381.0
89	381.0
90	240.0
91	99.0
92	99.0
93	99.0
94	60.0
95	21.0
96	21.0
97	21.0
98	17.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11055825765557187
2	0.049068619414947626
3	0.03681545219502342
4	0.03194775562820422
5	0.036423798448267854
6	0.02003029161978478
7	0.02165285714205785
8	0.02484203765135319
9	0.030660893317435923
10	0.03776661129428695
11	0.021820708747810235
12	0.02198856035356262
13	0.016897061645740234
14	0.02372302694633728
15	0.023163521593829325
16	0.02372302694633728
17	0.02769551494914376
18	0.020981450719048302
19	0.03524883720800115
20	0.025009889257105577
21	0.029989486894426376
22	0.03860586932304888
23	0.030604942782185125
24	0.04095579180358229
25	0.032954865262718534
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1787293.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.0286393160863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.71563058479114	64.93468778944572
2	8.810639377535676	13.044792892473914
3	1.9627314628690207	4.358950186171955
4	0.6306164120309418	1.867346996521722
5	0.2809342923978072	1.0398591701718596
6	0.15062986830803085	0.6690554514722877
7	0.09871797767002666	0.5115570294063505
8	0.059447587867087856	0.3520659232339196
9	0.0442920850709907	0.29509945112500036
>10	0.21285715043204378	2.8560317090030587
>50	0.01663708499163423	0.8356612994618229
>100	0.01247678918056336	1.8441396695291383
>500	0.0016171204202863232	0.8511040186467275
>1k	0.0023101720289804614	3.1516501523712646
>5k	3.0802293719739486E-4	1.3646737509969196
>10k+	1.5401146859869743E-4	2.023324509968406
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	19875	1.11201688810956	No Hit
CCCATGTACTCTGCGTTGATACCAC	15618	0.8738354595469239	No Hit
GAGTACATGGGAAGCAGTGGTATCA	7988	0.4469328755833542	No Hit
GTATCAACGCAGAGTACATGGGAAG	5432	0.30392330748232105	No Hit
GCGTTGATACCACTGCTTCCCATGT	5404	0.30235669249529873	No Hit
CATGTACTCTGCGTTGATACCACTG	5115	0.28618698780781887	No Hit
GCTTCCCATGTACTCTGCGTTGATA	4313	0.2413146585366809	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	4289	0.2399718456906618	No Hit
TATCAACGCAGAGTACATGGGAAGC	4056	0.22693537097722644	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3536	0.19784109264681282	No Hit
GGTATCAACGCAGAGTACATGGGAA	2574	0.14401667773554758	No Hit
CAGTGGTATCAACGCAGAGTACATG	2070	0.11581760796914664	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.595053525079548E-5	0.0	0.0	0.0
3	0.0	5.595053525079548E-5	0.0	5.595053525079548E-5	0.0
4	0.0	5.595053525079548E-5	0.0	1.1190107050159096E-4	0.0
5	0.0	5.595053525079548E-5	0.0	1.1190107050159096E-4	0.0
6	0.0	5.595053525079548E-5	0.0	1.1190107050159096E-4	0.0
7	0.0	5.595053525079548E-5	0.0	1.1190107050159096E-4	0.0
8	0.0	5.595053525079548E-5	0.0	1.6785160575238643E-4	0.0
9	5.595053525079548E-5	5.595053525079548E-5	0.0	1.6785160575238643E-4	0.0
10	5.595053525079548E-5	5.595053525079548E-5	0.0	1.6785160575238643E-4	0.0
11	5.595053525079548E-5	5.595053525079548E-5	0.0	4.4760428200636384E-4	0.0
12	5.595053525079548E-5	5.595053525079548E-5	0.0	4.4760428200636384E-4	0.0
13	5.595053525079548E-5	5.595053525079548E-5	0.0	4.4760428200636384E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCAAT	25	0.00603684	18.997591	13
GCGTTTA	300	0.0	13.931568	13
TAGGACC	465	0.0	13.894214	4
GTATCGA	80	2.0014668E-6	13.061576	18
GTAGGAC	1070	0.0	12.608349	3
TGCGTTT	355	0.0	12.307955	12
CGTGAAA	430	0.0	12.148943	9
AGGACGT	770	0.0	12.092085	5
TGTAGGA	1250	0.0	12.009504	2
CCAACGA	95	1.0409385E-6	11.999152	19
GGACGTG	760	0.0	11.998816	6
GGCGAGG	305	0.0	11.835228	19
TAGGACG	825	0.0	11.631761	4
GTCCTAA	480	0.0	11.494443	1
TGGCGAG	870	0.0	11.464706	18
TTAGGAC	520	0.0	11.327708	3
AGGACCT	740	0.0	11.298396	5
CGTTTAT	370	0.0	11.295865	14
GACGTGA	415	0.0	11.214818	7
GTATAGG	255	0.0	11.191344	1
>>END_MODULE