FastQCFastQC Report
Thu 26 May 2016
SRR1513054_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513054_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1787293
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC228751.2798684938619465No Hit
CCCATGTACTCTGCGTTGATACCAC220441.2333735990685355No Hit
GAGTACATGGGAAGCAGTGGTATCA83760.4686416832606629No Hit
CATGTACTCTGCGTTGATACCACTG65840.36837832409123744No Hit
GCGTTGATACCACTGCTTCCCATGT58860.3293248504861822No Hit
GTATCAACGCAGAGTACATGGGAAG57230.3202049132403025No Hit
GCTTCCCATGTACTCTGCGTTGATA55650.3113647286706768No Hit
ACGCAGAGTACATGGGAAGCAGTGG53510.2993913141270066No Hit
TATCAACGCAGAGTACATGGGAAGC48570.2717517497131136No Hit
ACTCTGCGTTGATACCACTGCTTCC45020.25188930969908124No Hit
GGTATCAACGCAGAGTACATGGGAA33800.18911280914768872No Hit
CAGTGGTATCAACGCAGAGTACATG25970.14530354004631585No Hit
GTATCAACGCAGAGTACTTTTTTTT24320.1360717017299346No Hit
ATACCACTGCTTCCCATGTACTCTG22910.12818267625957244No Hit
CATGGGAAGCAGTGGTATCAACGCA20940.11716042081516573No Hit
ACATGGGAAGCAGTGGTATCAACGC20810.11643306385690538No Hit
CCACTGCTTCCCATGTACTCTGCGT20220.11313198227710845No Hit
GTGGTATCAACGCAGAGTACATGGG20090.11240462531884811No Hit
GCAGTGGTATCAACGCAGAGTACAT19790.11072610926132424No Hit
GTCCTACAGTGGACATTTCTAAATT19170.10725717607577494No Hit
GGGAAGCAGTGGTATCAACGCAGAG18930.10591436322975584No Hit
TACCACTGCTTCCCATGTACTCTGC18550.10378824289022562No Hit
CTGCTTCCCATGTACTCTGCGTTGA18320.10250138057945732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGA350.002193157616.26026710
ACCGTCC602.60134E-514.230928
GCGCCAC1153.8380676E-1013.20449813
TAGGACC5550.012.35380654
GCACCGC550.003014320712.1198336
TCCAACG1103.8835424E-812.07539718
GACCGCG550.003099049212.0750597
CGTTCAC550.003104899812.07201810
ACACCGT951.0104595E-612.0287066
TCTATAC2550.011.9500873
GTAGGAC12100.011.6476323
GTCCTAC11350.011.4197931
AGGACGT8350.011.4044615
TGTAGGA12550.011.3878212
TGGCGAG8800.011.32068418
TTAGGAC5900.011.2981483
GGACGTG8300.011.2436996
GGCGAGA5400.011.241981519
GTCCTAG2600.010.9967181
TAGGACA5350.010.8576874
Produced by FastQC (version 0.11.5)