FastQCFastQC Report
Thu 26 May 2016
SRR1513053_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513053_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143247
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC172340.8041070394592876No Hit
CCCATGTACTCTGCGTTGATACCAC139270.6498084448502669No Hit
GAGTACATGGGAAGCAGTGGTATCA70910.33085314011870776No Hit
GCGTTGATACCACTGCTTCCCATGT47990.2239125961683371No Hit
GTATCAACGCAGAGTACATGGGAAG46660.21770705849582433No Hit
CATGTACTCTGCGTTGATACCACTG44950.20972851005973647No Hit
TATCAACGCAGAGTACATGGGAAGC36050.16820273164968855No Hit
ACGCAGAGTACATGGGAAGCAGTGG35770.16689630266600164No Hit
GCTTCCCATGTACTCTGCGTTGATA35300.1647033683005272No Hit
ACTCTGCGTTGATACCACTGCTTCC31680.14781310786857513No Hit
GGTATCAACGCAGAGTACATGGGAA23640.11029993276556552No Hit
GTCCTACAGTGGACATTTCTAAATT22040.10283462428735464No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGAAC500.001501676513.2978768
TAGGACC6200.012.7195714
GTAGGAC11600.012.2040563
CCCTTAT1501.2732926E-1112.0499741
TGTAGGA12050.011.9856852
GCGTTTA2550.011.91938613
GTCCTAA6800.011.7515221
TAGAACA6250.011.4016394
TAGGACT3250.011.4016394
CGTTTAT2800.011.1943814
AATCCCG1701.0913936E-1111.17572919
GTAGAAC4850.011.1662923
GTATTAG2650.011.1285751
TGCACCG951.3592864E-511.0010675
GACGTGG4250.010.9511937
ACTGTTC5650.010.9274588
GTCCTAC11650.010.6971751
CGTAGGA803.7652988E-410.6895352
CTTAGAC2400.010.68878653
TCCTAAA7050.010.3778632
Produced by FastQC (version 0.11.5)