##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513053_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2143247
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.241786410992294	31.0	30.0	34.0	24.0	34.0
2	28.968634506428796	31.0	30.0	34.0	16.0	34.0
3	29.304608381581776	31.0	30.0	34.0	23.0	34.0
4	32.14803706712292	35.0	33.0	37.0	19.0	37.0
5	32.38749803452425	35.0	33.0	37.0	22.0	37.0
6	32.823729602794266	37.0	33.0	37.0	26.0	37.0
7	32.822054574204465	37.0	35.0	37.0	26.0	37.0
8	32.80447657222896	37.0	35.0	37.0	26.0	37.0
9	34.16692103150034	39.0	35.0	39.0	25.0	39.0
10	33.67299522640181	38.0	34.0	39.0	19.0	39.0
11	34.05132679527838	39.0	34.0	39.0	25.0	39.0
12	33.87926496572724	39.0	34.0	39.0	23.0	39.0
13	33.97492729489415	39.0	35.0	39.0	25.0	39.0
14	34.27466083003966	39.0	33.0	41.0	17.0	41.0
15	34.82614649641408	40.0	34.0	41.0	22.0	41.0
16	34.40858799755698	39.0	34.0	41.0	17.0	41.0
17	33.9414075932452	39.0	33.0	41.0	11.0	41.0
18	33.35907433907524	39.0	32.0	41.0	10.0	41.0
19	34.23698201840479	39.0	34.0	41.0	18.0	41.0
20	34.50660889762123	39.0	34.0	41.0	18.0	41.0
21	34.52440199379726	39.0	34.0	41.0	18.0	41.0
22	34.11771345066621	39.0	34.0	41.0	10.0	41.0
23	32.45837507296173	39.0	31.0	41.0	2.0	41.0
24	33.548592859339124	39.0	33.0	41.0	2.0	41.0
25	33.279888412301524	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.71354181384471
1101	2	1.146877564745921
1101	3	0.9788613764486271
1101	4	0.9516459321251602
1101	5	1.231079968555207
1101	6	0.8188704182903805
1101	7	0.7602042671388602
1101	8	0.7646100984638693
1101	9	0.8787106454262101
1101	10	1.2559157177603808
1101	11	0.9528417682309964
1101	12	0.9561813662598126
1101	13	0.8927394281430452
1101	14	0.020321418572649463
1101	15	0.5685381816765798
1101	16	1.1564733538766916
1101	17	1.7395130289684388
1101	18	-4.751586357506003
1101	19	-0.953986800513178
1101	20	0.12445468759257494
1101	21	0.6279021337283481
1101	22	1.2176416632696245
1101	23	3.067625476471413
1101	24	2.0320904927505765
1101	25	2.204304165064272
1102	1	1.0805290698182404
1102	2	1.4177955141050909
1102	3	1.1838201434545006
1102	4	1.6131404452397646
1102	5	1.52310332583248
1102	6	1.0066352614385465
1102	7	1.0528648454618263
1102	8	0.9854055144167617
1102	9	1.1452052236237478
1102	10	1.4835009596341848
1102	11	1.107883703331872
1102	12	1.257713339530845
1102	13	1.1785542683920625
1102	14	2.0692312400600343
1102	15	1.5795540591502757
1102	16	1.8424176499890308
1102	17	1.413122828012078
1102	18	1.8296345745841052
1102	19	1.5244319959101773
1102	20	1.4166820699926532
1102	21	1.4733175541226586
1102	22	1.904391409939251
1102	23	3.492936109404873
1102	24	2.363225337874894
1102	25	2.5791917984004584
1103	1	0.75398381170859
1103	2	1.0575726508334036
1103	3	0.7676989097291553
1103	4	1.2551215602784822
1103	5	1.0879699329307542
1103	6	0.8294187596318636
1103	7	0.661534638160667
1103	8	0.6540860168641416
1103	9	0.7762987486228425
1103	10	1.088173048176479
1103	11	0.9459505780168271
1103	12	1.0346337529082987
1103	13	0.9218971860604768
1103	14	1.6849024130915708
1103	15	1.269906098949079
1103	16	1.4470626500910981
1103	17	1.8097199702994047
1103	18	1.8054854264173983
1103	19	1.2711699980866769
1103	20	1.2218631856595081
1103	21	1.2466929151055695
1103	22	1.5040387891130322
1103	23	3.087406063221046
1103	24	1.898909057957873
1103	25	2.0128404307090406
1104	1	0.31805941880994837
1104	2	0.7478083768707613
1104	3	0.5126387167481568
1104	4	1.0468402163326758
1104	5	0.9293459133503887
1104	6	0.5274112314009969
1104	7	0.4739539958057648
1104	8	0.48236550485164287
1104	9	0.5171858966915792
1104	10	0.9530296077324394
1104	11	0.3708227392222696
1104	12	0.5522706733321527
1104	13	0.5280502678212429
1104	14	1.2494999118011023
1104	15	0.7430258295257985
1104	16	1.0498211647831397
1104	17	1.6157332752004905
1104	18	2.170057792358243
1104	19	1.199800378190801
1104	20	0.6790876000176667
1104	21	0.8778818110617053
1104	22	1.1875533361528738
1104	23	2.6593328572657384
1104	24	1.7100579541460093
1104	25	1.7379699128000112
1105	1	0.48976680746649137
1105	2	0.8409536860904261
1105	3	0.4607815525891894
1105	4	0.6110086522342968
1105	5	0.7122012573867096
1105	6	0.40851722218440756
1105	7	0.19975737048830666
1105	8	0.3022083920537426
1105	9	0.5428763716986253
1105	10	0.9401836601680671
1105	11	0.522028304205854
1105	12	0.4376091001686646
1105	13	0.5305951259155748
1105	14	1.286211951356222
1105	15	0.6424305618446908
1105	16	1.051254540956812
1105	17	1.5222722255885373
1105	18	1.5491914707811532
1105	19	1.0935009549968981
1105	20	0.8743193047080879
1105	21	0.6856555572717582
1105	22	1.2208324681744926
1105	23	2.6922432310181037
1105	24	1.6279787066211568
1105	25	1.6919092120646226
1106	1	-0.4923169374079528
1106	2	-0.07927656452242005
1106	3	-0.3250706054310122
1106	4	-2.406780157902599
1106	5	-0.4924086788811408
1106	6	-0.49389612246449843
1106	7	-0.5052687895266033
1106	8	-0.284293252662458
1106	9	-0.28498937852271666
1106	10	-0.12021821636284358
1106	11	-0.294983960443318
1106	12	-0.1793737169641929
1106	13	-0.321411118888868
1106	14	0.40850825902387555
1106	15	-0.10720771645075189
1106	16	-1.0967512828929742
1106	17	-5.8842167518681805
1106	18	-7.086606185196548
1106	19	-2.9835657054906157
1106	20	-1.6506166590125702
1106	21	-1.16875939781837
1106	22	-1.4836718432315479
1106	23	-4.777240549193358
1106	24	-2.0111997173284557
1106	25	-0.6143733431301612
1107	1	0.2977268772866033
1107	2	0.7200145860364948
1107	3	0.3565489293232318
1107	4	0.7136873080063424
1107	5	0.8365519754812567
1107	6	0.34189169186752366
1107	7	0.32289186727301455
1107	8	0.3075385275477558
1107	9	0.1526557477756043
1107	10	0.6367145722270209
1107	11	0.3178009393194543
1107	12	0.336805312646284
1107	13	0.3390129074285113
1107	14	1.1063878418255726
1107	15	0.4467366628214009
1107	16	-5.7768593620591275
1107	17	-1.1179256197788021
1107	18	0.5871582741191546
1107	19	0.47061955844574754
1107	20	0.3603610059846645
1107	21	0.28827255505346017
1107	22	0.3730765165497232
1107	23	2.2362762964532834
1107	24	1.2742080529394357
1107	25	1.0196438137377157
1108	1	0.6749005860008204
1108	2	0.8960290759932903
1108	3	0.7620654829733837
1108	4	1.2847354576426042
1108	5	1.0687726183754407
1108	6	0.6697271495189341
1108	7	0.6499918683559329
1108	8	0.7305928418390977
1108	9	0.7488825020121368
1108	10	1.1601551909583208
1108	11	0.7565506705575586
1108	12	0.809030350138741
1108	13	0.7468079325544537
1108	14	1.424811102911825
1108	15	0.9516951635784707
1108	16	1.2462965055857183
1108	17	1.40418301361683
1108	18	1.110303544007003
1108	19	1.0555862190129162
1108	20	0.9262436515143122
1108	21	0.9144995693746907
1108	22	1.2980654074162388
1108	23	3.0268076957561263
1108	24	1.7834248157587638
1108	25	1.9357709435050765
1109	1	0.2351940505286656
1109	2	0.5666671706861273
1109	3	0.35265876905608806
1109	4	0.3418832035846009
1109	5	0.16610424192604967
1109	6	0.09557375414205183
1109	7	0.12477173938851394
1109	8	0.06420891557542063
1109	9	0.17088487473402125
1109	10	0.6237052097922415
1109	11	0.15415288000890826
1109	12	0.2728030779925845
1109	13	0.07101649566068602
1109	14	-1.0080689934793128
1109	15	-0.19272894924939976
1109	16	-0.41599815327020195
1109	17	0.6142343978867899
1109	18	1.4034188151941436
1109	19	0.6201992565323451
1109	20	0.4452003208990689
1109	21	0.36972801565318036
1109	22	0.16587806206884892
1109	23	-4.411299855654544
1109	24	-1.4426983037905856
1109	25	-0.15527331907885866
1110	1	0.3965162169455745
1110	2	0.7687785266982488
1110	3	0.571200465128971
1110	4	1.0316848871658095
1110	5	0.8143899833178239
1110	6	0.4719959100038764
1110	7	0.3968004150558997
1110	8	0.30089655417771866
1110	9	0.42222975862181755
1110	10	0.7524485507858785
1110	11	0.4394522218020924
1110	12	0.5729283151038516
1110	13	0.3491224463280105
1110	14	1.1953944107095822
1110	15	0.7467566601414575
1110	16	1.0913777714793156
1110	17	1.4802197421544605
1110	18	2.058257967561751
1110	19	1.2344129744815007
1110	20	0.7519267605785132
1110	21	0.6779749755472224
1110	22	0.9531658722936882
1110	23	2.2976060942296073
1110	24	1.4284844873914793
1110	25	1.751170144535358
1111	1	0.44656743326095594
1111	2	0.7325202855725053
1111	3	0.626678277577124
1111	4	1.0802340107886579
1111	5	0.7893001448982631
1111	6	0.32805658194367737
1111	7	0.22151508331047154
1111	8	0.037382519994643815
1111	9	0.23734105563141128
1111	10	0.5189602698382316
1111	11	0.31709891110662625
1111	12	0.3948450063878113
1111	13	0.3133977640934589
1111	14	-0.41451824732720866
1111	15	0.0605451667646264
1111	16	0.5653730823257987
1111	17	1.0897908432893146
1111	18	1.7831155868937856
1111	19	0.828701578268003
1111	20	0.663147107141242
1111	21	0.5241059589598862
1111	22	0.7594825311009004
1111	23	0.5520349903395712
1111	24	0.6505230784137268
1111	25	0.2647483444056462
1112	1	0.3980783194439894
1112	2	0.6709998941801274
1112	3	0.466998527257644
1112	4	0.9890777985578296
1112	5	0.6465200713060781
1112	6	0.24728321278011123
1112	7	0.27657480004208423
1112	8	0.32074230979270624
1112	9	0.4186557617017854
1112	10	0.539348973754251
1112	11	0.2699623927614567
1112	12	0.342119472742894
1112	13	0.10420489437456837
1112	14	0.5902492932491583
1112	15	0.35734010098391167
1112	16	0.6985206926707619
1112	17	0.8859277015756604
1112	18	1.1282592374318625
1112	19	0.47472363919573723
1112	20	0.49479915554073983
1112	21	0.3916519719995293
1112	22	0.44167397473966474
1112	23	2.061999926811197
1112	24	1.0892186601940779
1112	25	1.126736408825991
1113	1	0.6140957123924089
1113	2	0.9195341972178817
1113	3	0.7203878105584707
1113	4	0.8829772389356734
1113	5	0.9477271021085087
1113	6	0.4721299455226955
1113	7	0.42174694406671165
1113	8	0.3599394108343503
1113	9	0.5535457296236359
1113	10	0.8497448580778482
1113	11	0.5406451631934388
1113	12	0.5902041539673775
1113	13	0.42425462000839076
1113	14	1.0909450212072542
1113	15	0.705752996110796
1113	16	1.115122193124897
1113	17	1.6221754037777103
1113	18	2.256600543813718
1113	19	1.2277537428125171
1113	20	0.9123691300232011
1113	21	0.8841259842929787
1113	22	1.1123907173398635
1113	23	1.0238983196303408
1113	24	1.0746635736719412
1113	25	1.4635241081619483
1114	1	-0.21035743962315578
1114	2	-2.7704893314583856
1114	3	-0.8918256758729797
1114	4	0.23261675460832265
1114	5	0.41149437334862426
1114	6	0.20313443049345636
1114	7	0.1592542133401338
1114	8	0.16845160790997227
1114	9	0.30978151261800235
1114	10	0.5382647135324561
1114	11	0.29237481069310434
1114	12	0.20491442824284434
1114	13	0.1362087244371608
1114	14	0.9458371363981257
1114	15	0.38372450902486577
1114	16	0.6335150015423423
1114	17	1.0216715527722542
1114	18	1.0806225863997767
1114	19	0.6244657519452659
1114	20	0.5586013508293775
1114	21	0.31039161933605186
1114	22	0.5348852845144663
1114	23	2.1659306424179903
1114	24	1.1774375870207052
1114	25	0.9804358733466714
1115	1	0.26166445418428097
1115	2	0.479058105072081
1115	3	0.2833808145479324
1115	4	0.4375122561572553
1115	5	0.4621527602043045
1115	6	0.05906590765999198
1115	7	-0.12884610617736314
1115	8	-0.032543388230642734
1115	9	-0.11348092459833481
1115	10	0.09403117414554174
1115	11	0.021604368274843466
1115	12	-0.1023348114160001
1115	13	-0.09202122269287827
1115	14	0.6753617587701299
1115	15	-0.06371111113383421
1115	16	0.46353508164300905
1115	17	0.9269231096313959
1115	18	1.3793982872492805
1115	19	0.5626474810939968
1115	20	0.20702961288834132
1115	21	0.25162303143138587
1115	22	0.5373983576111172
1115	23	2.2538952635218408
1115	24	1.0086475001931774
1115	25	0.6344204565252767
1116	1	0.21619450031484888
1116	2	0.1872180197015041
1116	3	0.19269952153846504
1116	4	0.8803977839136294
1116	5	0.30574510987592163
1116	6	0.1743274148485483
1116	7	0.02121286614925566
1116	8	0.0033760534999416336
1116	9	0.13473335017624066
1116	10	0.4867501120077051
1116	11	0.11543498481643866
1116	12	0.2307720456230058
1116	13	0.09029720219901094
1116	14	0.8533541849905433
1116	15	0.014395350617000702
1116	16	0.4523366116853538
1116	17	0.7095016370531582
1116	18	1.2636262602903372
1116	19	0.45764071160184017
1116	20	0.2396063170252205
1116	21	0.1752367295811723
1116	22	0.4864896178335414
1116	23	1.7639631509822777
1116	24	0.665007745114309
1116	25	0.9844316442013863
1201	1	1.0668666225675771
1201	2	1.4570584693108763
1201	3	1.192329746084777
1201	4	1.7196941099495504
1201	5	1.5309373545236582
1201	6	1.011243185570315
1201	7	1.0112401698050277
1201	8	1.031422615678757
1201	9	1.2765710939151447
1201	10	1.5810307729192488
1201	11	1.2154142814176296
1201	12	1.3587000917489647
1201	13	1.2928115075093842
1201	14	2.1501917659879766
1201	15	1.5604374278423592
1201	16	1.836245071120949
1201	17	2.262143803467552
1201	18	2.855918623152135
1201	19	2.029064417563916
1201	20	1.7010367574640242
1201	21	1.603334865704845
1201	22	2.0382430816262556
1201	23	3.7116340521706945
1201	24	2.490016544897678
1201	25	2.6296166611147527
1202	1	0.7838303197118215
1202	2	1.154781988775035
1202	3	0.9109448789988512
1202	4	1.553850463404551
1202	5	1.1924349044825249
1202	6	0.8378371690996644
1202	7	0.6296275980124904
1202	8	0.6529862554415757
1202	9	0.8306116081142108
1202	10	1.2259012661202746
1202	11	0.8580370657278138
1202	12	0.8919756524824294
1202	13	0.8934645145914217
1202	14	1.7318180559066079
1202	15	1.25088730549205
1202	16	1.4022719497454759
1202	17	1.8733733239037136
1202	18	2.6066778748951265
1202	19	1.6891664820407257
1202	20	1.379645747571189
1202	21	1.2772762532912765
1202	22	1.7042083298494717
1202	23	3.176363900182764
1202	24	1.9107454022910844
1202	25	2.1010287337941165
1203	1	1.1539898651105993
1203	2	1.471305775992601
1203	3	1.2618911815001752
1203	4	1.8428766286268896
1203	5	1.5008280663445888
1203	6	1.1881881737568847
1203	7	1.130471251966192
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2211	18	-5.713072821645984
2211	19	-1.9478410587961008
2211	20	-1.2190024618633402
2211	21	-0.9893083751758383
2211	22	-0.5951724997856687
2211	23	-4.887884479324313
2211	24	-2.290455326240835
2211	25	-1.5915610499970718
2212	1	0.22637122793045705
2212	2	0.5844879088106296
2212	3	0.23625954930668058
2212	4	0.8134684038749853
2212	5	0.6473145429373659
2212	6	0.3236343060542879
2212	7	0.21235935966536346
2212	8	0.1161441442546618
2212	9	-0.14237033094782703
2212	10	-3.727146035300102
2212	11	-1.1516006181366478
2212	12	-0.23180051501808663
2212	13	-0.04064769954440095
2212	14	0.8353266211630768
2212	15	0.28781739436827536
2212	16	0.43228950589034554
2212	17	1.0165871207562915
2212	18	1.5346944919860164
2212	19	0.8477594918100024
2212	20	0.35058375293876765
2212	21	0.27397830924921607
2212	22	0.5263138779401615
2212	23	1.857614072527113
2212	24	0.9931448558914795
2212	25	0.9759878635615848
2213	1	-1.4076819238508556
2213	2	-3.6646343480401065
2213	3	-1.6713937644413193
2213	4	-3.5010054305356135
2213	5	-1.278684858302796
2213	6	-0.8696690605698052
2213	7	-0.9935438953912517
2213	8	-0.8368769006458621
2213	9	-0.7274258023850422
2213	10	-0.7721219449089034
2213	11	-0.8329337418322424
2213	12	-0.7075896395579804
2213	13	-1.1002407582379448
2213	14	-5.897957490378559
2213	15	-2.684675406513925
2213	16	-1.9463886133145536
2213	17	-0.7970792494022092
2213	18	-0.15477659631383034
2213	19	-0.7549684407670583
2213	20	-0.9309487276284614
2213	21	-1.0519097829684156
2213	22	-0.8863665630744606
2213	23	-5.535639756412799
2213	24	-3.0341823706658886
2213	25	-2.11245241014489
2214	1	-0.04241354228103589
2214	2	0.15181311556371924
2214	3	-0.05770901445344734
2214	4	0.2595401271594113
2214	5	0.14396409552472988
2214	6	-0.0508609442707737
2214	7	-0.12408597322622938
2214	8	-0.30517748462965244
2214	9	-0.08476558646500365
2214	10	0.36615503929674986
2214	11	-0.22791725994966328
2214	12	-0.08246665286791455
2214	13	-0.07827048256975644
2214	14	0.4097281917900091
2214	15	0.0605490655491252
2214	16	0.4441516507751899
2214	17	0.7003159171776332
2214	18	1.244929408237411
2214	19	0.18099083010736194
2214	20	0.1016679794959714
2214	21	-0.017652269106974927
2214	22	0.5272208351923595
2214	23	1.9833613339433498
2214	24	1.0084030177181305
2214	25	0.8700023464019893
2215	1	-2.4300974762751757
2215	2	-4.4252741680680465
2215	3	-2.483410608600593
2215	4	-4.01804613187835
2215	5	-4.887583154333456
2215	6	-2.35090967442612
2215	7	-1.8223665176857722
2215	8	-2.3100312698264567
2215	9	-1.859338307903343
2215	10	-3.346939967111105
2215	11	-2.2299652510535957
2215	12	-1.8402729931502648
2215	13	-1.937550369447493
2215	14	-1.1695795974304843
2215	15	-1.8253402629777398
2215	16	-2.714214172653122
2215	17	-7.373692557416895
2215	18	-3.3291378345893357
2215	19	-2.4105762597145173
2215	20	-2.1048897891975358
2215	21	-2.0706370370035714
2215	22	-1.7688768185388355
2215	23	-6.358497901418517
2215	24	-3.658104140874947
2215	25	-3.5036420843285363
2216	1	-1.0528477718773104
2216	2	-0.5698545981330625
2216	3	-0.8880478416609421
2216	4	-3.030274916455653
2216	5	-1.2785854473323646
2216	6	-1.075269733746456
2216	7	-1.042194998109398
2216	8	-1.002474442768328
2216	9	-1.0221730676049035
2216	10	-0.5352968338543249
2216	11	-1.139111703124584
2216	12	-1.8470150911146987
2216	13	-1.2982901114795595
2216	14	-6.790007352416485
2216	15	-3.2422887604600312
2216	16	-1.712301217764562
2216	17	-0.8897525826050838
2216	18	-0.07635347347865462
2216	19	-0.6591069116384674
2216	20	-1.0236627645357643
2216	21	-1.3054367198189638
2216	22	-0.7867916036803209
2216	23	0.1665656798800299
2216	24	-0.49654944763111075
2216	25	-0.5365576135655701
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	105051.0
3	3.0
4	930.0
5	5468.0
6	6344.0
7	5742.0
8	5342.0
9	4928.0
10	4736.0
11	4729.0
12	4522.0
13	4483.0
14	4531.0
15	4606.0
16	4796.0
17	5002.0
18	5878.0
19	6710.0
20	8330.0
21	10266.0
22	12777.0
23	15532.0
24	18462.0
25	21596.0
26	24682.0
27	28678.0
28	31719.0
29	38230.0
30	44956.0
31	51225.0
32	66392.0
33	86604.0
34	113319.0
35	149094.0
36	210932.0
37	294799.0
38	731853.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.2028186975786	17.41052597158413	11.775793488593711	25.61086184224356
2	13.967474794342655	19.134173591930455	41.41719301472219	25.481158599004704
3	20.2130281246412	23.651573323923913	28.051736868586453	28.083661682848437
4	11.021474763465832	15.746510794321686	37.93293033536699	35.29908410684549
5	12.672231725502733	37.59294480603584	36.01700789018616	13.717815578275266
6	33.97076200236323	34.83560043083253	17.625913045750753	13.567724521053487
7	29.576231716866825	30.538467497533627	20.787774252831078	19.097526532768473
8	26.61399316769027	36.06106143478504	19.67056506561631	17.654380331908378
9	27.096193355617554	15.083201796677157	19.117557833346247	38.70304701435904
10	14.611201427891968	29.12998568467355	34.14497080224172	22.11384208519276
11	35.096998800640286	22.61096410787704	22.418413205090513	19.873623886392167
12	23.917292243592794	24.77093290996639	30.68392988311675	20.627844963324065
13	29.498712973488562	20.78519816848381	24.623598410396262	25.092490447631366
14	21.029698017036562	21.6740876319475	27.531602298484742	29.76461205253119
15	25.437825653757574	28.54977471118464	22.30020930509309	23.712190329964695
16	22.37522237550239	27.4845387190051	27.93367656474884	22.20656234074367
17	22.96357832247466	27.566598838837344	27.646405436182725	21.823417402505275
18	22.57990752511139	26.408263379832114	29.441137325396248	21.570691769660247
19	23.900608061056275	26.931065255014126	27.361394003774947	21.806932680154656
20	23.968922295471536	26.783052495895475	27.235553431605435	22.01247177702755
21	22.99444800182204	26.48162184059754	27.419991673869575	23.10393848371084
22	23.143098121639074	27.098844175344805	26.087340072337646	23.670717630678478
23	21.251859858604075	26.87381337754056	28.67985976152739	23.194467002327972
24	23.027955616048573	27.835003426070624	26.753270637174452	22.38377032070635
25	22.990474581381445	26.160620148671022	26.590475141453098	24.25843012849444
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	158.0
1	158.0
2	303.0
3	448.0
4	448.0
5	448.0
6	813.5
7	1179.0
8	1179.0
9	1179.0
10	1831.0
11	2483.0
12	2483.0
13	2483.0
14	4547.0
15	6611.0
16	6611.0
17	6611.0
18	12262.0
19	17913.0
20	17913.0
21	17913.0
22	29244.5
23	40576.0
24	40576.0
25	40576.0
26	58930.0
27	77284.0
28	77284.0
29	77284.0
30	102001.5
31	126719.0
32	126719.0
33	126719.0
34	149721.5
35	172724.0
36	172724.0
37	172724.0
38	194603.5
39	216483.0
40	216483.0
41	216483.0
42	238826.0
43	261169.0
44	261169.0
45	261169.0
46	298094.5
47	335020.0
48	335020.0
49	335020.0
50	317081.5
51	299143.0
52	299143.0
53	299143.0
54	261564.5
55	223986.0
56	223986.0
57	223986.0
58	190914.5
59	157843.0
60	157843.0
61	157843.0
62	130063.5
63	102284.0
64	102284.0
65	102284.0
66	79548.5
67	56813.0
68	56813.0
69	56813.0
70	41890.5
71	26968.0
72	26968.0
73	26968.0
74	18908.0
75	10848.0
76	10848.0
77	10848.0
78	7572.0
79	4296.0
80	4296.0
81	4296.0
82	2925.5
83	1555.0
84	1555.0
85	1555.0
86	1057.5
87	560.0
88	560.0
89	560.0
90	354.5
91	149.0
92	149.0
93	149.0
94	87.0
95	25.0
96	25.0
97	25.0
98	17.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1186050884475751
2	0.045258432649153364
3	0.033267280906027166
4	0.02981457573485464
5	0.03322062272803834
6	0.0195497765773147
7	0.019829725645247608
8	0.022722532680554318
9	0.029161361243011184
10	0.0367666442551885
11	0.020389623781113423
12	0.019829725645247608
13	0.015070591490388183
14	0.020716231027035147
15	0.020622914671057514
16	0.022162634544688503
17	0.026455186919659746
18	0.01842998030558307
19	0.03261406641418371
20	0.022815849036531954
21	0.02776161590334665
22	0.037046593323121414
23	0.02850814675116774
24	0.03970610946848403
25	0.030981030184575084
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2143247.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.23457475651233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.8006982153785	60.40741676427821
2	10.356160891361943	14.754334344123835
3	2.5675999059765817	5.4870566234130855
4	0.9459688279876555	2.6954274877846798
5	0.4242079515259311	1.510913651764045
6	0.23877705625295714	1.0205509238274735
7	0.1400691332842965	0.6984435602214076
8	0.0994132089848124	0.5665326133770721
9	0.062358284174105764	0.3997859270119359
>10	0.32736489114479644	4.081008975003826
>50	0.02085880807173969	1.0181793104500043
>100	0.01259438174129646	1.7326989526040417
>500	0.0015980111656116048	0.8018631735606894
>1k	0.0021306815541486065	3.0114691006572682
>5k	6.658379856714395E-5	0.3363309927669736
>10k+	1.331675971342879E-4	1.477987599155502
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	17234	0.8041070394592876	No Hit
CCCATGTACTCTGCGTTGATACCAC	13927	0.6498084448502669	No Hit
GAGTACATGGGAAGCAGTGGTATCA	7091	0.33085314011870776	No Hit
GCGTTGATACCACTGCTTCCCATGT	4799	0.2239125961683371	No Hit
GTATCAACGCAGAGTACATGGGAAG	4666	0.21770705849582433	No Hit
CATGTACTCTGCGTTGATACCACTG	4495	0.20972851005973647	No Hit
TATCAACGCAGAGTACATGGGAAGC	3605	0.16820273164968855	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	3577	0.16689630266600164	No Hit
GCTTCCCATGTACTCTGCGTTGATA	3530	0.1647033683005272	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3168	0.14781310786857513	No Hit
GGTATCAACGCAGAGTACATGGGAA	2364	0.11029993276556552	No Hit
GTCCTACAGTGGACATTTCTAAATT	2204	0.10283462428735464	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.33163559776358E-5
2	0.0	0.0	0.0	0.0	9.33163559776358E-5
3	0.0	0.0	0.0	0.0	9.33163559776358E-5
4	0.0	0.0	0.0	4.66581779888179E-5	9.33163559776358E-5
5	0.0	0.0	0.0	4.66581779888179E-5	1.399745339664537E-4
6	0.0	0.0	0.0	4.66581779888179E-5	2.3329088994408952E-4
7	0.0	0.0	0.0	4.66581779888179E-5	2.3329088994408952E-4
8	0.0	0.0	0.0	4.66581779888179E-5	2.3329088994408952E-4
9	0.0	0.0	0.0	1.399745339664537E-4	2.3329088994408952E-4
10	0.0	0.0	0.0	1.399745339664537E-4	2.799490679329074E-4
11	0.0	0.0	0.0	1.399745339664537E-4	2.799490679329074E-4
12	0.0	0.0	0.0	1.399745339664537E-4	4.1992360189936113E-4
13	0.0	0.0	0.0	1.866327119552716E-4	4.1992360189936113E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGAAC	50	0.0015016765	13.297876	8
TAGGACC	620	0.0	12.719571	4
GTAGGAC	1160	0.0	12.204056	3
CCCTTAT	150	1.2732926E-11	12.049974	1
TGTAGGA	1205	0.0	11.985685	2
GCGTTTA	255	0.0	11.919386	13
GTCCTAA	680	0.0	11.751522	1
TAGAACA	625	0.0	11.401639	4
TAGGACT	325	0.0	11.401639	4
CGTTTAT	280	0.0	11.19438	14
AATCCCG	170	1.0913936E-11	11.175729	19
GTAGAAC	485	0.0	11.166292	3
GTATTAG	265	0.0	11.128575	1
TGCACCG	95	1.3592864E-5	11.001067	5
GACGTGG	425	0.0	10.951193	7
ACTGTTC	565	0.0	10.927458	8
GTCCTAC	1165	0.0	10.697175	1
CGTAGGA	80	3.7652988E-4	10.689535	2
CTTAGAC	240	0.0	10.6887865	3
TCCTAAA	705	0.0	10.377863	2
>>END_MODULE