FastQCFastQC Report
Thu 26 May 2016
SRR1513053_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513053_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143247
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC193830.9043754639572573No Hit
CCCATGTACTCTGCGTTGATACCAC188570.8798332623351393No Hit
GAGTACATGGGAAGCAGTGGTATCA74450.34737013512674925No Hit
CATGTACTCTGCGTTGATACCACTG55940.26100584766944734No Hit
GCGTTGATACCACTGCTTCCCATGT50510.23567045702151923No Hit
GTATCAACGCAGAGTACATGGGAAG49600.2314245628245368No Hit
ACGCAGAGTACATGGGAAGCAGTGG46330.2161673386221933No Hit
GCTTCCCATGTACTCTGCGTTGATA43470.2028230997173914No Hit
TATCAACGCAGAGTACATGGGAAGC40360.18831240636286906No Hit
ACTCTGCGTTGATACCACTGCTTCC39730.18537294114957353No Hit
GTATCAACGCAGAGTACTTTTTTTT29460.13745499235505754No Hit
GGTATCAACGCAGAGTACATGGGAA28370.1323692509542764No Hit
GTCCTACAGTGGACATTTCTAAATT22390.10446766051696327No Hit
CAGTGGTATCAACGCAGAGTACATG21660.10106161352377958No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTC350.00218971216.2643098
AGGACCG851.6725608E-814.5589055
GACCGCG551.9822989E-413.8003437
TAGGACC6050.012.2727954
GTAGGAC11350.012.16123
GCACCGT1001.8839819E-611.423146
TGTAGGA13050.011.3865732
GGCGAGG3250.011.38448519
CCTACAC3450.011.3127723
GACGTGG4950.011.1169427
ACGTGGA5300.011.0986018
CTTATAC2500.011.0423693
TAGGACG9150.011.0278034
TCCAACG951.3753801E-510.9893918
CGTGGAA5100.010.9754969
AGGACGT9200.010.9678715
GTCCTAG2800.010.8881041
GTCCTAC12200.010.8546371
TAGGACT2900.010.8322894
GGACGTG8950.010.7424326
Produced by FastQC (version 0.11.5)