FastQCFastQC Report
Thu 26 May 2016
SRR1513052_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513052_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523938
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC105640.6932040542331774No Hit
CCCATGTACTCTGCGTTGATACCAC81820.5368984827466734No Hit
GAGTACATGGGAAGCAGTGGTATCA43220.2836073383562848No Hit
GCGTTGATACCACTGCTTCCCATGT27550.18078163284858045No Hit
GTATCAACGCAGAGTACATGGGAAG27380.17966610190178342No Hit
GCTTCCCATGTACTCTGCGTTGATA27250.17881304882482096No Hit
CATGTACTCTGCGTTGATACCACTG26400.17323539409083571No Hit
ACGCAGAGTACATGGGAAGCAGTGG23320.15302459811357155No Hit
TATCAACGCAGAGTACATGGGAAGC20430.1340605720180217No Hit
ACTCTGCGTTGATACCACTGCTTCC18640.12231468734292339No Hit
GTATCAACGCAGAGTACTTTTTTTT18220.11955866970966011No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTC250.006037323718.9971127
CGTGCGC456.7700184E-414.77553210
GTAGGAC7150.013.1548693
TAGGACC3850.013.0802274
TAGGACT2550.013.0415154
CCAACGA604.0915268E-412.66681919
CGTTTAT2250.012.66474114
TGTAGGA8250.012.5533152
TCGAACT1001.442113E-712.35014919
GCGTTTA2200.012.08907113
GGACGTG4700.011.9248926
GTCCTAC9400.011.8370461
GTCCTAG2500.011.7925311
GTCTACG657.952012E-411.7047451
TCTACGA658.013611E-411.6939852
GACGTGG2850.011.6648937
GGATAGG907.372395E-611.6234631
TAGGACG5000.011.2119774
CGTTTCC855.3307172E-511.17477114
CTGTAGG8700.011.1497791