##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513052_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1523938
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.345963549698215	31.0	30.0	34.0	25.0	34.0
2	29.07888509900009	31.0	30.0	34.0	16.0	34.0
3	29.409162315002316	31.0	30.0	34.0	23.0	34.0
4	32.25701373677932	35.0	33.0	37.0	19.0	37.0
5	32.501820940221975	35.0	33.0	37.0	23.0	37.0
6	32.93276694983654	37.0	33.0	37.0	26.0	37.0
7	32.939410264722056	37.0	35.0	37.0	26.0	37.0
8	32.913971565772364	37.0	35.0	37.0	26.0	37.0
9	34.28733780508131	39.0	35.0	39.0	26.0	39.0
10	33.78370839233617	39.0	34.0	39.0	22.0	39.0
11	34.16362870405489	39.0	35.0	39.0	26.0	39.0
12	33.9972603872336	39.0	34.0	39.0	25.0	39.0
13	34.09163955489003	39.0	35.0	39.0	25.0	39.0
14	34.406454199580295	39.0	34.0	41.0	17.0	41.0
15	34.95231236441378	40.0	34.0	41.0	24.0	41.0
16	34.52523855957394	39.0	34.0	41.0	17.0	41.0
17	34.071417603603294	39.0	33.0	41.0	16.0	41.0
18	33.49063413340963	39.0	32.0	41.0	10.0	41.0
19	34.38562592441425	39.0	34.0	41.0	18.0	41.0
20	34.64685243100441	39.0	34.0	41.0	18.0	41.0
21	34.672438773755886	40.0	34.0	41.0	18.0	41.0
22	34.26039510793746	39.0	34.0	41.0	11.0	41.0
23	32.60428573865866	39.0	31.0	41.0	7.0	41.0
24	33.688018803914595	39.0	33.0	41.0	2.0	41.0
25	33.42595499291966	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6944074588086337
1101	2	1.081979834876229
1101	3	0.7499711749970679
1101	4	0.7836550301166767
1101	5	1.0525453195822578
1101	6	0.6564421545106072
1101	7	0.7697673741711384
1101	8	0.7668251857405579
1101	9	0.8450696643148845
1101	10	1.0961479157883645
1101	11	0.8642786532953011
1101	12	0.9909639530971077
1101	13	0.9514733568108085
1101	14	0.08270967432802934
1101	15	0.686296842613821
1101	16	1.3382623347796425
1101	17	1.845755097925121
1101	18	-4.704323975535615
1101	19	-0.8057547871146653
1101	20	0.25262260347680154
1101	21	0.5548375752525772
1101	22	1.1282307274872991
1101	23	3.0298581402992895
1101	24	1.8510103686938209
1101	25	2.0981865082002997
1102	1	0.8896078450068607
1102	2	1.1715255359555883
1102	3	0.9331326799317594
1102	4	1.5065893339909238
1102	5	1.2507786461502448
1102	6	0.7836358762667075
1102	7	0.7578832932667368
1102	8	0.655550100915697
1102	9	0.9081531040017978
1102	10	1.2528706660518125
1102	11	0.893492347737741
1102	12	0.9728293776915606
1102	13	0.7188703371718006
1102	14	1.6082064401471925
1102	15	1.2058389546181942
1102	16	1.361825816113587
1102	17	0.9828933094191044
1102	18	1.4849962903540685
1102	19	1.1112963004438612
1102	20	1.0990331271492195
1102	21	1.101993777988298
1102	22	1.4561529282668033
1102	23	3.0420265951967735
1102	24	2.008595414291122
1102	25	2.1698963412337733
1103	1	0.8291449162166238
1103	2	1.149055716415596
1103	3	0.8859698609442219
1103	4	1.4065343944359014
1103	5	1.3464025081029618
1103	6	0.9731211789805982
1103	7	0.8256369837300994
1103	8	0.8253412159899938
1103	9	0.9780176501086189
1103	10	1.1927687795390511
1103	11	0.8589927523343235
1103	12	1.0642598004510546
1103	13	1.0010464631461389
1103	14	1.7058250129260557
1103	15	1.3018933929166394
1103	16	1.5608709070465707
1103	17	2.0411257992507075
1103	18	1.9954974628818434
1103	19	1.4740517139767135
1103	20	1.401459331253868
1103	21	1.354544188277508
1103	22	1.6846621254845218
1103	23	3.254762256469583
1103	24	2.141368043236703
1103	25	2.291083142597863
1104	1	0.5285518618550249
1104	2	0.9186476295395991
1104	3	0.7696670281490761
1104	4	1.2387009258405328
1104	5	1.0354919561766494
1104	6	0.5436545081931143
1104	7	0.6565571585290897
1104	8	0.6526089344572483
1104	9	0.7162266740127947
1104	10	1.0602747575865408
1104	11	0.7369467205101614
1104	12	0.8589476847506958
1104	13	0.5652329698335024
1104	14	1.6646139732551717
1104	15	0.9248429521989934
1104	16	1.3767046915712982
1104	17	1.843715542458888
1104	18	2.3594038302703026
1104	19	1.5499953335580798
1104	20	1.0202026039234227
1104	21	1.1387402033703822
1104	22	1.3425798762505963
1104	23	2.8799050065583884
1104	24	1.8533722907314925
1104	25	1.880474555042504
1105	1	0.569894245072259
1105	2	0.9963054524781683
1105	3	0.7187002960031315
1105	4	0.8713088191922651
1105	5	0.8155327042469551
1105	6	0.45582689413849664
1105	7	0.34965651907161543
1105	8	0.38430848774889625
1105	9	0.6053637238053184
1105	10	1.05284865489174
1105	11	0.6459174188615364
1105	12	0.5125448975092866
1105	13	0.5550113120899525
1105	14	1.308946907571233
1105	15	0.7123517456520361
1105	16	1.1424426085663413
1105	17	1.6684160782205595
1105	18	1.3794771513316562
1105	19	0.9601070040922721
1105	20	0.7744678850583284
1105	21	0.5820643036950486
1105	22	1.0585331333718173
1105	23	2.6173589468398646
1105	24	1.6464133996212666
1105	25	1.5679906697415262
1106	1	-0.3741029699938103
1106	2	-0.21473413593186663
1106	3	-0.4432686205529244
1106	4	-2.7316116214936876
1106	5	-0.6547643245209329
1106	6	-0.4625498354189972
1106	7	-0.5370787975512314
1106	8	-0.4009967925001945
1106	9	-0.3121324196165105
1106	10	-0.14742538079372025
1106	11	-0.22522819876801492
1106	12	-0.1520120394109412
1106	13	-0.18017497947698757
1106	14	0.5653168489467149
1106	15	-0.19431877932425579
1106	16	-1.4248199490887217
1106	17	-5.913027089502016
1106	18	-7.070052626627284
1106	19	-2.8545944022200302
1106	20	-1.5675438746009647
1106	21	-1.1505869500068826
1106	22	-1.3556785673172982
1106	23	-4.838915947143192
1106	24	-2.056600709589926
1106	25	-0.6020878746885003
1107	1	0.4743184490768648
1107	2	0.8532371323035619
1107	3	0.6955297795974893
1107	4	0.8476452507947911
1107	5	0.8978619586956285
1107	6	0.509009366368872
1107	7	0.42035578388444605
1107	8	0.43704273089579004
1107	9	0.4458042323715219
1107	10	0.9555880663943981
1107	11	0.5096204680084853
1107	12	0.6374604886548454
1107	13	0.49472084134879424
1107	14	1.2842493021207702
1107	15	0.7464486235077814
1107	16	-5.645184988075048
1107	17	-1.0866238004465743
1107	18	0.7568532914572188
1107	19	0.7021595822785187
1107	20	0.5273169297357967
1107	21	0.41728760868017645
1107	22	0.5044330721748267
1107	23	2.5053838208097403
1107	24	1.4717802385359633
1107	25	1.1957005575836348
1108	1	0.174015846586002
1108	2	0.4233637547563376
1108	3	0.29252071793881385
1108	4	0.7427954029168404
1108	5	0.5528633814198187
1108	6	0.21878567035031438
1108	7	0.1824905268091257
1108	8	0.23859295305964423
1108	9	0.13571875947989298
1108	10	0.6825820690647362
1108	11	0.1941302848053823
1108	12	0.29782175115840204
1108	13	0.06071061962269653
1108	14	0.7456365676448797
1108	15	0.4482281231466274
1108	16	0.7172222612518908
1108	17	0.8911920990434297
1108	18	0.8316962445948732
1108	19	0.6059117437248815
1108	20	0.4263998198358223
1108	21	0.5051446577313286
1108	22	0.7790412758213776
1108	23	2.4400430236464317
1108	24	1.2441263107228053
1108	25	1.3152591847642299
1109	1	0.10818079944340298
1109	2	0.41821517079582904
1109	3	0.3106377369822795
1109	4	0.23210794888142772
1109	5	0.011121666788511675
1109	6	0.01537779548848306
1109	7	0.09199809472661968
1109	8	-0.025071831719671422
1109	9	0.12732201153389155
1109	10	0.6017220157201351
1109	11	0.3001427827686314
1109	12	0.28056163674786916
1109	13	0.09814670958337501
1109	14	-1.053885180484606
1109	15	-0.2585761767164101
1109	16	-0.30353648284518897
1109	17	0.662280210124699
1109	18	1.2808819833530904
1109	19	0.5366413714135803
1109	20	0.3112318371691529
1109	21	0.3040159290034836
1109	22	0.2979216189061731
1109	23	-4.5010171673900885
1109	24	-1.5711057838236897
1109	25	-0.2526096805692575
1110	1	0.6189310738693337
1110	2	0.915391663172965
1110	3	0.7100579765160973
1110	4	1.3342743454418695
1110	5	0.9688875735204192
1110	6	0.6933824804534936
1110	7	0.6153985829737039
1110	8	0.5599661169889032
1110	9	0.5574303357548374
1110	10	0.9023011260336418
1110	11	0.6247239482674516
1110	12	0.6976948465287691
1110	13	0.5314160058071664
1110	14	1.3860162001409506
1110	15	0.7815897281880382
1110	16	1.2982616988710731
1110	17	1.4382629027091411
1110	18	2.2079583996623597
1110	19	1.3840758426136617
1110	20	0.9485132431014591
1110	21	0.9273905692889457
1110	22	1.1474868336999222
1110	23	2.596728893870811
1110	24	1.7325227182368579
1110	25	2.012811809014856
1111	1	0.2819233029311121
1111	2	0.5513822275097766
1111	3	0.34268029688738366
1111	4	0.7148951420796763
1111	5	0.6411900260716621
1111	6	0.12437996362722714
1111	7	0.035337035488709034
1111	8	-0.20910452008925517
1111	9	0.046239798488393546
1111	10	0.3551020458792138
1111	11	0.10882925130772492
1111	12	0.1969598963449073
1111	13	0.13077527211821405
1111	14	-0.5955718493862037
1111	15	-0.16673082391071148
1111	16	0.2287079480445655
1111	17	0.7390162074214857
1111	18	1.4432126978947366
1111	19	0.4846055091884409
1111	20	0.4111222145693958
1111	21	0.2391401848094219
1111	22	0.49283155960135616
1111	23	0.3946711700750214
1111	24	0.3738694210953284
1111	25	-0.026279918681190395
1112	1	0.35254088494862046
1112	2	0.7022216293592045
1112	3	0.46231528881780193
1112	4	1.0223438046545041
1112	5	0.7963276879749657
1112	6	0.2277798132969977
1112	7	0.2809895218165863
1112	8	0.3082708151271234
1112	9	0.2703926231899345
1112	10	0.7045552044253469
1112	11	0.36597320339018324
1112	12	0.4947532819889773
1112	13	0.2618188130135195
1112	14	0.7038115876366646
1112	15	0.3016115980434293
1112	16	0.6720364997269002
1112	17	0.7786194261955188
1112	18	0.9274871629203147
1112	19	0.4622178017767098
1112	20	0.2901098632774364
1112	21	0.393420285041195
1112	22	0.3653285307869041
1112	23	2.009233518362116
1112	24	0.8501535643915688
1112	25	0.825315053704557
1113	1	0.6181654995743457
1113	2	0.9302660861356244
1113	3	0.7261378933313054
1113	4	0.9945136159752579
1113	5	0.9919005082980661
1113	6	0.5381880017296012
1113	7	0.5701497141954235
1113	8	0.4772898063714166
1113	9	0.6466597338077662
1113	10	0.8222500496590399
1113	11	0.5819831922914034
1113	12	0.5892146829015417
1113	13	0.5334213534007475
1113	14	1.3008889091313023
1113	15	0.7999606546472009
1113	16	1.097452703944434
1113	17	1.606912064005364
1113	18	2.0326218897670287
1113	19	1.1151050258487274
1113	20	0.9215610286492435
1113	21	0.8972046604265671
1113	22	1.047827914188062
1113	23	1.1808792206681957
1113	24	1.0141426505920847
1113	25	1.466474567824676
1114	1	-0.35862697180016667
1114	2	-2.8413622558752323
1114	3	-1.0667080308185923
1114	4	0.03723555239525922
1114	5	0.22574233681155675
1114	6	0.010104376774542345
1114	7	-0.045134626687747925
1114	8	-0.06701762112405163
1114	9	0.18534584537150778
1114	10	0.13832516391526894
1114	11	-0.07728074412342067
1114	12	-0.07156668874222305
1114	13	0.015535113032022707
1114	14	0.664500466404256
1114	15	0.09834092549976958
1114	16	0.3796481305915478
1114	17	0.8242332953603224
1114	18	0.9056123832866874
1114	19	0.3674950631194065
1114	20	0.15072963515001447
1114	21	0.0031621172781868268
1114	22	0.09684218748741102
1114	23	1.8418463314275542
1114	24	0.7432524154583291
1114	25	0.5843888387962224
1115	1	-0.06255345467402051
1115	2	0.22845702917950916
1115	3	0.0307714134060042
1115	4	-0.01659749513584785
1115	5	-0.03839827788307559
1115	6	-0.4443750234804824
1115	7	-0.42632554257395583
1115	8	-0.4927848901738656
1115	9	-0.45338695153303377
1115	10	-0.3166038252242487
1115	11	-0.3055751643845994
1115	12	-0.5573656158846347
1115	13	-0.6876570779718065
1115	14	0.30968909587266324
1115	15	-0.42841236122581705
1115	16	0.036621704878143646
1115	17	0.584736527123944
1115	18	0.9516111521386463
1115	19	0.14345469905133967
1115	20	-0.35286707592592137
1115	21	-0.37460906506363045
1115	22	-0.05398114276805188
1115	23	1.7574665222554984
1115	24	0.5973009138974064
1115	25	0.2976720468162668
1116	1	0.04966876754820149
1116	2	0.12268983341231277
1116	3	0.18201479964939082
1116	4	0.9265506530122991
1116	5	0.26959114010634266
1116	6	0.10274137863591903
1116	7	-0.03852130976972035
1116	8	-0.12662086900984804
1116	9	-0.062381660527748295
1116	10	0.3955125768921519
1116	11	0.06632959752015921
1116	12	0.09816348464446634
1116	13	-0.055805226119950646
1116	14	0.6972287784123523
1116	15	0.026349543536092312
1116	16	0.42466403292046806
1116	17	0.605027532414951
1116	18	1.1870608875883804
1116	19	0.5538250694217126
1116	20	0.25194773888889443
1116	21	0.022798342343776312
1116	22	0.36525166146475385
1116	23	1.694027368816343
1116	24	0.7920892736857681
1116	25	0.927830776975
1201	1	1.118683488434371
1201	2	1.4020448027235553
1201	3	1.1618863765170317
1201	4	1.8156584928153734
1201	5	1.5829636643577487
1201	6	1.1531400895462198
1201	7	1.020624263437945
1201	8	1.1017596715657092
1201	9	1.1881195621641183
1201	10	1.6173649145818345
1201	11	1.3240893992983302
1201	12	1.521212926740695
1201	13	1.3245714601421739
1201	14	2.207137926738639
1201	15	1.55087463705987
1201	16	1.897520435453302
1201	17	2.3042354548104527
1201	18	2.8207885592915645
1201	19	2.002287230752259
1201	20	1.7145817896427573
1201	21	1.7193835502013926
1201	22	2.005584136484593
1201	23	3.5993009764861057
1201	24	2.3679967377742557
1201	25	2.4397629540143058
1202	1	0.975008000586552
1202	2	1.2870326718259477
1202	3	0.9797565236743004
1202	4	1.6479796926714272
1202	5	1.3583358139837571
1202	6	0.9919678617562084
1202	7	0.7655167105514877
1202	8	0.777404040855771
1202	9	1.0311122758865423
1202	10	1.502457840740071
1202	11	1.1314033438328437
1202	12	1.1279307740577735
1202	13	1.0971511816152173
1202	14	1.9544952484221838
1202	15	1.3732030341948942
1202	16	1.5556342066340036
1202	17	2.0364959034408443
1202	18	2.6451074297057175
1202	19	1.7938971769274517
1202	20	1.5336619309570736
1202	21	1.4148206301182782
1202	22	1.7816102324250949
1202	23	3.384001147905167
1202	24	2.178270853495661
1202	25	2.372471878253272
1203	1	0.8721164813805977
1203	2	1.165130495623636
1203	3	0.8950902680212671
1203	4	1.6911636953059954
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2211	18	-5.1103489504695325
2211	19	-1.6809505511537424
2211	20	-0.8638414629462474
2211	21	-0.6716603821723695
2211	22	-0.337861697998072
2211	23	-4.9285570203308
2211	24	-2.099358786351303
2211	25	-1.3577676187956698
2212	1	0.2645804673274519
2212	2	0.4826591736134418
2212	3	0.27765988181765167
2212	4	0.8386085387125704
2212	5	0.6947340147690824
2212	6	0.370382413710729
2212	7	0.18850285015179225
2212	8	0.1412593124961674
2212	9	-0.05686902869061328
2212	10	-3.776276115774021
2212	11	-1.118228901376085
2212	12	-0.18141034415121737
2212	13	-0.07711623911970378
2212	14	0.7753781529524488
2212	15	0.27186094898127067
2212	16	0.4981230714291769
2212	17	1.0798484791895362
2212	18	1.6354359182481772
2212	19	0.7391500632897561
2212	20	0.48646331406672516
2212	21	0.30223052286867613
2212	22	0.5489884777512373
2212	23	1.852328815958849
2212	24	0.9972995999060288
2212	25	0.9475971493075974
2213	1	-1.3962681656176947
2213	2	-3.6013721482009444
2213	3	-1.5040427480615683
2213	4	-3.300920317330167
2213	5	-1.1427047221016196
2213	6	-0.8913939328999803
2213	7	-0.9316354284928821
2213	8	-0.7461317444730042
2213	9	-0.6719595096200379
2213	10	-0.6780418314299368
2213	11	-0.7889752720207923
2213	12	-0.7388772731497468
2213	13	-1.1519657147793936
2213	14	-6.394412540596068
2213	15	-2.853744405582553
2213	16	-2.102733607499353
2213	17	-0.6920853150495105
2213	18	-0.03557449371171373
2213	19	-0.6070476031671603
2213	20	-0.7847958234260375
2213	21	-0.8244230272049293
2213	22	-0.7906972091304141
2213	23	-5.713491971369383
2213	24	-3.1428382490651146
2213	25	-2.1207855664508877
2214	1	-0.3542627872887678
2214	2	-0.1268418890943508
2214	3	-0.19603274668433102
2214	4	0.052483165961788814
2214	5	-0.07432813891399093
2214	6	-0.3735582111721598
2214	7	-0.3388093423455487
2214	8	-0.4009174048342743
2214	9	-0.3653039037086785
2214	10	0.01658386645172527
2214	11	-0.3985073736466447
2214	12	-0.406495936296281
2214	13	-0.5203436853552574
2214	14	-0.037347705913532536
2214	15	-0.37926439700105163
2214	16	0.19928033879789098
2214	17	0.37474788332365705
2214	18	0.8393573635307732
2214	19	-0.07631086279182853
2214	20	-0.23409405876971334
2214	21	-0.2773621415030476
2214	22	0.19552771763409993
2214	23	1.6338680826101708
2214	24	0.6621066394751622
2214	25	0.5712871678849041
2215	1	-2.6148271737977
2215	2	-4.675328554181881
2215	3	-2.650049731496054
2215	4	-4.012392642207509
2215	5	-5.066702246760492
2215	6	-2.5084415953828767
2215	7	-1.9474788611938862
2215	8	-2.1909242061254375
2215	9	-2.01426551610367
2215	10	-3.4986964947089056
2215	11	-2.4621569172742888
2215	12	-2.0858718940177816
2215	13	-2.1150988166468494
2215	14	-1.406346328509187
2215	15	-2.0161834057525567
2215	16	-2.7938955085534474
2215	17	-7.531444784363732
2215	18	-3.604960002115149
2215	19	-2.7455083802888005
2215	20	-2.392519690048289
2215	21	-2.3611805933869263
2215	22	-2.1580438492855905
2215	23	-6.618533801938071
2215	24	-4.061418334472275
2215	25	-3.880766587081908
2216	1	-1.144971931770474
2216	2	-0.7261809154700067
2216	3	-1.0165609955827861
2216	4	-3.1031692481460915
2216	5	-1.5862863648571626
2216	6	-1.1670200874194165
2216	7	-1.2604575769277382
2216	8	-1.06323754353242
2216	9	-1.1293454233650166
2216	10	-0.7270932993272936
2216	11	-1.2437218229709046
2216	12	-2.0002164842175745
2216	13	-1.418715054575479
2216	14	-7.107675879427134
2216	15	-3.5482015984273154
2216	16	-1.8706325633286909
2216	17	-1.0126295500435205
2216	18	-0.1990934310186958
2216	19	-0.6826733379714085
2216	20	-1.3390392488701721
2216	21	-1.3810644792128386
2216	22	-0.8571032541913581
2216	23	0.2542230523517901
2216	24	-0.49322746225573155
2216	25	-0.5823933306652265
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	70335.0
3	2.0
4	576.0
5	3578.0
6	4296.0
7	3957.0
8	3617.0
9	3445.0
10	3360.0
11	3276.0
12	3141.0
13	3213.0
14	3172.0
15	3145.0
16	3253.0
17	3464.0
18	3971.0
19	4683.0
20	5908.0
21	7419.0
22	9123.0
23	10961.0
24	13192.0
25	15327.0
26	17686.0
27	20092.0
28	22613.0
29	27508.0
30	32562.0
31	36521.0
32	47359.0
33	62071.0
34	80920.0
35	106696.0
36	151007.0
37	210525.0
38	521964.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.95014459316175	17.68326411343567	12.155548343780916	25.211042949621664
2	13.698160698142972	19.160789395768628	42.44830594764715	24.692743958441245
3	20.33881950128889	23.52621459662454	28.120784530988765	28.014181371097806
4	10.771813930774098	15.872058371776124	38.60205214916415	34.75407554828563
5	12.436229011040947	37.83716900132597	36.232686980609415	13.493915007023672
6	33.488490538298365	35.41384443324302	17.794444269422215	13.303220759036396
7	29.329941496057515	30.568342764583655	20.782354045330383	19.31936169402845
8	25.993796280130898	36.99732607334462	19.58431401376475	17.42456363275974
9	26.541268301391913	15.693874309328804	19.447423660239	38.31743372904028
10	14.24688215296372	29.670075035758664	34.43649661580917	21.646546195468446
11	35.01685755708828	22.890311351236406	22.497373082645673	19.595458009029638
12	23.408290951858714	25.2056723903808	31.067671877143276	20.31836478061721
13	29.17466524119763	21.087819262076945	24.960343477658792	24.777172019066633
14	20.65545128647394	22.042737634400133	27.992704227163955	29.309106851961968
15	24.92693258632719	29.116634166736127	22.56901941660273	23.38741383033395
16	22.04933456813055	28.174904518714595	28.125810171587368	21.64995074156749
17	22.57395432121131	28.042058197265785	27.87448441360334	21.509503067919564
18	21.85329234420368	27.2385630352258	29.722653932383686	21.185490688186835
19	23.339477460374976	27.47225624685737	27.766727976652316	21.421538316115335
20	23.306213318736308	27.310550420780284	28.07472837323231	21.3085078872511
21	22.751137993390287	26.837937270062977	27.842653337840456	22.56827139870628
22	22.556699948141294	27.67141272310732	26.84252676631416	22.92936056243723
23	20.996334765120405	27.261628456505527	29.016005209044415	22.72603156932965
24	22.36085555445419	28.181989453597073	27.546159385517438	21.910995606431296
25	22.700870835764718	26.810084024788	27.131585010361153	23.357460129086125
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	121.0
1	121.0
2	234.0
3	347.0
4	347.0
5	347.0
6	690.0
7	1033.0
8	1033.0
9	1033.0
10	1623.0
11	2213.0
12	2213.0
13	2213.0
14	3914.5
15	5616.0
16	5616.0
17	5616.0
18	10087.0
19	14558.0
20	14558.0
21	14558.0
22	23443.5
23	32329.0
24	32329.0
25	32329.0
26	46261.0
27	60193.0
28	60193.0
29	60193.0
30	78633.5
31	97074.0
32	97074.0
33	97074.0
34	114558.5
35	132043.0
36	132043.0
37	132043.0
38	147530.0
39	163017.0
40	163017.0
41	163017.0
42	177608.5
43	192200.0
44	192200.0
45	192200.0
46	213883.0
47	235566.0
48	235566.0
49	235566.0
50	221755.0
51	207944.0
52	207944.0
53	207944.0
54	179829.0
55	151714.0
56	151714.0
57	151714.0
58	127915.5
59	104117.0
60	104117.0
61	104117.0
62	84205.0
63	64293.0
64	64293.0
65	64293.0
66	49194.0
67	34095.0
68	34095.0
69	34095.0
70	24823.5
71	15552.0
72	15552.0
73	15552.0
74	10918.0
75	6284.0
76	6284.0
77	6284.0
78	4308.5
79	2333.0
80	2333.0
81	2333.0
82	1613.5
83	894.0
84	894.0
85	894.0
86	600.5
87	307.0
88	307.0
89	307.0
90	188.5
91	70.0
92	70.0
93	70.0
94	41.0
95	12.0
96	12.0
97	12.0
98	12.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11391539550821622
2	0.045736768818678976
3	0.034843937220543096
4	0.03031619396589625
5	0.033990884143580646
6	0.021129468521685266
7	0.018373450888421967
8	0.022048141066106364
9	0.027560176332632955
10	0.034843937220543096
11	0.01896402609554982
12	0.01817659248604602
13	0.015026858048030825
14	0.020735751716933367
15	0.022638716273234213
16	0.022376238403399612
17	0.028216371007219455
18	0.01850468982333927
19	0.03490955668800174
20	0.02231061893594096
21	0.027757034735008904
22	0.03727185751651314
23	0.028478848877054055
24	0.03773119378872369
25	0.030119335563520297
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1523938.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.20036176808505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.90770842378276	69.52614962711235
2	8.162313467717567	12.765917320800527
3	1.6063715127092038	3.7685650028341717
4	0.5311543277428948	1.6614584233671355
5	0.24684781738104344	0.9651794310429893
6	0.12908081873775493	0.6056500033567828
7	0.08696140635366133	0.47602894057024353
8	0.058602881484069974	0.3666213226165188
9	0.04119165943814183	0.2899082402901401
>10	0.20300979247032375	2.771607199803925
>50	0.0133065270624528	0.713850311720239
>100	0.00953534253879327	1.3976865810747465
>500	0.002298534993437072	1.2914436494020693
>1k	0.0014472257366085267	2.1519622643910457
>5k	8.513092568285451E-5	0.5446977648027079
>10k+	8.513092568285451E-5	0.7032739168144472
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	10564	0.6932040542331774	No Hit
CCCATGTACTCTGCGTTGATACCAC	8182	0.5368984827466734	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4322	0.2836073383562848	No Hit
GCGTTGATACCACTGCTTCCCATGT	2755	0.18078163284858045	No Hit
GTATCAACGCAGAGTACATGGGAAG	2738	0.17966610190178342	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2725	0.17881304882482096	No Hit
CATGTACTCTGCGTTGATACCACTG	2640	0.17323539409083571	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2332	0.15302459811357155	No Hit
TATCAACGCAGAGTACATGGGAAGC	2043	0.1340605720180217	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1864	0.12231468734292339	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1822	0.11955866970966011	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.56194674586499E-5	0.0
5	0.0	0.0	0.0	6.56194674586499E-5	0.0
6	0.0	0.0	0.0	6.56194674586499E-5	0.0
7	0.0	6.56194674586499E-5	0.0	6.56194674586499E-5	0.0
8	0.0	6.56194674586499E-5	0.0	6.56194674586499E-5	0.0
9	0.0	6.56194674586499E-5	0.0	1.312389349172998E-4	0.0
10	0.0	6.56194674586499E-5	0.0	1.9685840237594968E-4	0.0
11	0.0	6.56194674586499E-5	0.0	1.9685840237594968E-4	0.0
12	0.0	6.56194674586499E-5	0.0	1.9685840237594968E-4	0.0
13	0.0	6.56194674586499E-5	0.0	1.9685840237594968E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTC	25	0.0060373237	18.997112	7
CGTGCGC	45	6.7700184E-4	14.775532	10
GTAGGAC	715	0.0	13.154869	3
TAGGACC	385	0.0	13.080227	4
TAGGACT	255	0.0	13.041515	4
CCAACGA	60	4.0915268E-4	12.666819	19
CGTTTAT	225	0.0	12.664741	14
TGTAGGA	825	0.0	12.553315	2
TCGAACT	100	1.442113E-7	12.350149	19
GCGTTTA	220	0.0	12.089071	13
GGACGTG	470	0.0	11.924892	6
GTCCTAC	940	0.0	11.837046	1
GTCCTAG	250	0.0	11.792531	1
GTCTACG	65	7.952012E-4	11.704745	1
TCTACGA	65	8.013611E-4	11.693985	2
GACGTGG	285	0.0	11.664893	7
GGATAGG	90	7.372395E-6	11.623463	1
TAGGACG	500	0.0	11.211977	4
CGTTTCC	85	5.3307172E-5	11.174771	14
CTGTAGG	870	0.0	11.149779	1
>>END_MODULE