FastQCFastQC Report
Thu 26 May 2016
SRR1513052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513052_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523938
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC121780.7991138747114385No Hit
CCCATGTACTCTGCGTTGATACCAC106010.6956319745291475No Hit
GAGTACATGGGAAGCAGTGGTATCA46080.3023745060494587No Hit
GCTTCCCATGTACTCTGCGTTGATA34360.22546849018792106No Hit
CATGTACTCTGCGTTGATACCACTG32650.2142475612524919No Hit
GTATCAACGCAGAGTACATGGGAAG30800.20210795977264168No Hit
GCGTTGATACCACTGCTTCCCATGT28960.19003397776025008No Hit
ACGCAGAGTACATGGGAAGCAGTGG28040.1839969867540543No Hit
GTATCAACGCAGAGTACTTTTTTTT27780.1822908806001294No Hit
TATCAACGCAGAGTACATGGGAAGC24850.163064376634745No Hit
ACTCTGCGTTGATACCACTGCTTCC21810.1431160585273154No Hit
TATCAACGCAGAGTACTTTTTTTTT19730.12946720929591624No Hit
GGTATCAACGCAGAGTACTTTTTTT17250.11319358136617107No Hit
GGTATCAACGCAGAGTACATGGGAA17200.11286548402887782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1550.013.46946918
CCAACGA1001.0279109E-813.28056119
CCAACGT655.5276752E-513.13462219
CGGTTTC802.0145217E-613.05369213
GTAGGAC8800.011.6823153
TGTAGGA10500.011.6151152
TGTAGAA4850.011.1978842
TAGGACG6600.010.96118454
TCTGGAC2450.010.8787693
GTCCTAC9300.010.6599151
GTTCTAG1803.274181E-1110.5915811
AGGACGT6600.010.5285055
CTGTAGG11050.010.5244871
TAGAACA5000.010.4708164
TAGGGGT1002.3461798E-510.4708164
CTTAGGC1105.9019367E-610.3842793
GTAGAAC4050.010.3415463
TTTACTC1752.2737368E-1010.334833
CCTACAC2400.010.3121663
TAGGACC4550.010.2511484
Produced by FastQC (version 0.11.5)