FastQCFastQC Report
Thu 26 May 2016
SRR1513051_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513051_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608736
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC81870.5089088576373003No Hit
CCCATGTACTCTGCGTTGATACCAC65240.40553577467030016No Hit
GAGTACATGGGAAGCAGTGGTATCA33460.2079893779961411No Hit
CATGTACTCTGCGTTGATACCACTG22690.14104240844986374No Hit
GCGTTGATACCACTGCTTCCCATGT22260.1383695025162612No Hit
GTATCAACGCAGAGTACATGGGAAG21340.13265072703041392No Hit
GTATCAACGCAGAGTACTTTTTTTT20820.1294183756688481No Hit
GCTTCCCATGTACTCTGCGTTGATA18330.11394038549519622No Hit
ACGCAGAGTACATGGGAAGCAGTGG17640.10965130388081076No Hit
TATCAACGCAGAGTACATGGGAAGC16380.10181906788932428No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA400.005281273314.2491618
CCGACGG400.005283452614.24827513
GCGTTTA1152.5465852E-1114.04177713
GCACCGT655.458916E-513.1522536
GTAGGAC4900.012.4089753
TAGGACC2750.012.0917014
GTATAAG2150.011.9458731
GTCCTAC5400.011.802491
TACACCG907.4606287E-611.6111495
TGGCGAG3850.011.10324218
ACGATAT600.00588110811.0819916
CCGCTCA600.00588246911.0816479
AACAGCG600.00588246911.0816477
ATGGCGA3800.010.99935117
TGCGTTT1803.45608E-1110.55427712
GTTCTAG2001.8189894E-1210.4636991
TATGGCG4300.010.16219216
CCGTGCG750.002651002510.1317919
CGCAGAT1502.4492692E-810.1317918
ACTCCGC750.002651002510.1317918
Produced by FastQC (version 0.11.5)