FastQCFastQC Report
Thu 26 May 2016
SRR1513051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513051_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608736
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC95760.5952499353529728No Hit
CCCATGTACTCTGCGTTGATACCAC86300.5364460048137171No Hit
GAGTACATGGGAAGCAGTGGTATCA36820.228875340640105No Hit
GTATCAACGCAGAGTACTTTTTTTT32560.20239492371650786No Hit
CATGTACTCTGCGTTGATACCACTG25670.15956626817576036No Hit
GTATCAACGCAGAGTACATGGGAAG24230.15061514132834722No Hit
TATCAACGCAGAGTACTTTTTTTTT23970.1489989656475643No Hit
ACGCAGAGTACATGGGAAGCAGTGG22940.14259642352753965No Hit
GCTTCCCATGTACTCTGCGTTGATA22870.14216129930579038No Hit
GCGTTGATACCACTGCTTCCCATGT21700.1348885087422672No Hit
TATCAACGCAGAGTACATGGGAAGC21570.13408042090187575No Hit
GGTATCAACGCAGAGTACTTTTTTT20380.12668330913213854No Hit
ACTCTGCGTTGATACCACTGCTTCC17620.1095269826745967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCGT602.5183255E-514.2797626
GCGATTG400.00530877214.238008513
GTCCTAT1153.601599E-1013.2574031
GTAGGAC4500.012.9046743
TAGGACA3150.012.3909034
TAGGACC3450.011.8653094
GTCCTAC5200.011.7277041
TACGCTG657.884451E-411.7167275
TTAGACT1800.011.6353614
TAGACAG2250.011.4238095
ACCGTGC855.389172E-511.1628998
TTAGGAC2000.010.9478183
ACTGTTC3300.010.9261118
AATCCCG700.001509381210.841619
GTATTAG1158.47871E-710.7716411
TGTACCG909.315445E-510.57760055
TGTAGGA6450.010.4857472
ATTAGAC1201.4831021E-610.3131613
GTGTAGA2600.010.2617411
TCCTACA7150.010.2585372
Produced by FastQC (version 0.11.5)