FastQCFastQC Report
Thu 26 May 2016
SRR1513050_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513050_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923644
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC59440.30899688299914124No Hit
CCCATGTACTCTGCGTTGATACCAC46100.2396493322049194No Hit
GTATCAACGCAGAGTACTTTTTTTT36730.19093969570253125No Hit
GAGTACATGGGAAGCAGTGGTATCA24520.1274664127042218No Hit
TATCAACGCAGAGTACTTTTTTTTT21100.1096876553042039No Hit
GGTATCAACGCAGAGTACTTTTTTT20240.10521697361881928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGG3600.012.4011997
GTATACG550.003043821312.1041631
ACCGATG550.003071828312.089378
CTGATCG550.003071828312.089379
GTCTACG657.951518E-411.7051251
GTAGGAC10150.011.6068493
TGTAGGA11750.011.3206522
GGACGTG8150.011.3056046
GTCCTAC9850.010.9104241
TAGTACA2450.010.8594324
CGTGCGC1053.4722962E-610.85576110
GTATTAT2850.010.678361
AGGACGT8550.010.6680835
GTTCTAA2150.010.6162761
TAGGACC4950.010.557784
CTGTAGG12250.010.48086551
GACGTGA4450.010.4593437
ATAGTAC3000.010.4508443
GGCGAGG3750.010.38669519
TAGGACG9150.010.3847034
Produced by FastQC (version 0.11.5)