##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513050_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1923644
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.157683542277052	31.0	30.0	34.0	23.0	34.0
2	28.87005911696759	31.0	30.0	34.0	16.0	34.0
3	29.22642495181021	31.0	30.0	34.0	22.0	34.0
4	32.10171268696287	35.0	32.0	37.0	19.0	37.0
5	32.31441056661212	35.0	33.0	37.0	20.0	37.0
6	32.815595297258746	36.0	33.0	37.0	26.0	37.0
7	32.78896562981508	37.0	35.0	37.0	26.0	37.0
8	32.7733296805438	37.0	35.0	37.0	26.0	37.0
9	34.133734724304496	39.0	35.0	39.0	25.0	39.0
10	33.62887779651536	38.0	34.0	39.0	18.0	39.0
11	34.004885519358055	39.0	34.0	39.0	25.0	39.0
12	33.86692496116745	39.0	34.0	39.0	23.0	39.0
13	33.934103711497556	39.0	35.0	39.0	23.0	39.0
14	34.25927406526363	39.0	33.0	41.0	17.0	41.0
15	34.782574634391814	40.0	34.0	41.0	22.0	41.0
16	34.355585025087805	39.0	34.0	41.0	17.0	41.0
17	33.88772558747876	39.0	33.0	41.0	11.0	41.0
18	33.29209562684156	39.0	32.0	41.0	10.0	41.0
19	34.203095791113114	39.0	33.0	41.0	18.0	41.0
20	34.484355213334695	39.0	34.0	41.0	18.0	41.0
21	34.51573472014572	40.0	34.0	41.0	18.0	41.0
22	34.09658387934566	39.0	34.0	41.0	10.0	41.0
23	32.392427081102326	39.0	31.0	41.0	2.0	41.0
24	33.50443533210927	39.0	33.0	41.0	2.0	41.0
25	33.23923501437896	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7855344802016937
1101	2	1.1520513441315678
1101	3	0.8572777255457815
1101	4	0.8086138372894709
1101	5	1.1484425349765246
1101	6	0.7643044269460262
1101	7	0.8369123175467195
1101	8	0.8539561325811391
1101	9	0.9995357377405085
1101	10	1.3387407926062025
1101	11	0.9006603652696157
1101	12	0.9680440261160115
1101	13	0.9151265690118038
1101	14	-0.18924015351419854
1101	15	0.4597314181628107
1101	16	1.170871878902446
1101	17	1.8405912867246812
1101	18	-4.053983525957129
1101	19	-0.6829398370871189
1101	20	0.28400398673060323
1101	21	0.7351425539338265
1101	22	1.2270236546275868
1101	23	3.1616928518142373
1101	24	2.039009446257012
1101	25	2.2926250686659486
1102	1	0.8964766983591232
1102	2	1.2456690356456157
1102	3	0.9235544328942211
1102	4	1.532269185695192
1102	5	1.2704210523856432
1102	6	0.9569048999747451
1102	7	0.9044831957132544
1102	8	0.8511520402313835
1102	9	0.8925012640888852
1102	10	1.2955151760493564
1102	11	1.0082950727456605
1102	12	1.1370323008817138
1102	13	0.9960009629701574
1102	14	1.874460105720118
1102	15	1.307396186238023
1102	16	1.618820268575135
1102	17	1.2821096159760543
1102	18	1.596041139206875
1102	19	1.343563265121361
1102	20	1.352442091619018
1102	21	1.3627863894101822
1102	22	1.6931423448640288
1102	23	3.3408769873230284
1102	24	2.136478526990018
1102	25	2.4336772385445684
1103	1	0.5346868946594121
1103	2	0.7970925221415008
1103	3	0.5705910705077386
1103	4	0.9844773429711537
1103	5	0.8894122657229815
1103	6	0.5203739521820197
1103	7	0.4493594104354841
1103	8	0.3585582362723798
1103	9	0.537725763025179
1103	10	0.8933397256665927
1103	11	0.6456360317942753
1103	12	0.7058272728598567
1103	13	0.5459202270356016
1103	14	1.3571151393404506
1103	15	0.860720208419778
1103	16	1.0896787213917563
1103	17	1.50018945615426
1103	18	1.466373040508671
1103	19	1.0541735580690457
1103	20	0.9512699472843877
1103	21	0.8035692333187399
1103	22	1.2301114471629404
1103	23	2.6741985343885446
1103	24	1.6778203346499154
1103	25	1.8169534084789802
1104	1	0.5987219823787129
1104	2	1.0595250457582353
1104	3	0.803553283733109
1104	4	1.3946241041047571
1104	5	1.1670438446703528
1104	6	0.77859763639254
1104	7	0.671365145928057
1104	8	0.6907782092818238
1104	9	0.6908043730116802
1104	10	1.0642911426706405
1104	11	0.5940250331438008
1104	12	0.7957311190137091
1104	13	0.5341709017994276
1104	14	1.5084393633620437
1104	15	1.0076922057207227
1104	16	1.2513268320530244
1104	17	1.8440912780166059
1104	18	2.372842338754282
1104	19	1.5737838819556842
1104	20	0.9327223764341852
1104	21	1.0780378568005204
1104	22	1.3588479518862897
1104	23	2.8006492766287536
1104	24	1.735369255027777
1104	25	1.7962550279121743
1105	1	0.6676603242756691
1105	2	0.976868123108602
1105	3	0.7105974442663694
1105	4	0.8761657302677861
1105	5	0.9427381926775737
1105	6	0.6548518024805858
1105	7	0.5182258250004139
1105	8	0.5165072746894097
1105	9	0.6986079766778417
1105	10	1.150209765084199
1105	11	0.6506656038978633
1105	12	0.7555639547309667
1105	13	0.7824115975361323
1105	14	1.5440759068976604
1105	15	0.8699118627128044
1105	16	1.2984340046679392
1105	17	1.7827343484234959
1105	18	1.6143964631814498
1105	19	1.235493193162462
1105	20	0.9747295629360551
1105	21	0.878429295489596
1105	22	1.3525267812155448
1105	23	2.994188368421561
1105	24	1.8610436405297932
1105	25	1.7663928559916116
1106	1	-0.6721920521204829
1106	2	-0.1942627594643156
1106	3	-0.32923287068345886
1106	4	-2.9801654622717706
1106	5	-0.7270181091070818
1106	6	-0.6030127601540087
1106	7	-0.6080204143749128
1106	8	-0.4163577747574436
1106	9	-0.47017470059484623
1106	10	-0.399445416943756
1106	11	-0.3972816415856286
1106	12	-0.34534669397734064
1106	13	-0.4607854224647667
1106	14	0.3446080148212829
1106	15	-0.2421924806261373
1106	16	-1.2778199351921558
1106	17	-6.362887018740043
1106	18	-7.217977259932134
1106	19	-3.172112827908542
1106	20	-1.6215135519896933
1106	21	-1.2516156284304998
1106	22	-1.5997977030511237
1106	23	-5.399165338742279
1106	24	-2.4158954433249455
1106	25	-0.99798282056679
1107	1	0.5613135005442054
1107	2	0.8517218270816223
1107	3	0.5915592237800773
1107	4	0.8969094681053065
1107	5	0.9341352937655287
1107	6	0.5056965105464286
1107	7	0.48708641267761266
1107	8	0.4527170129839959
1107	9	0.3447871399684459
1107	10	0.8646503083012576
1107	11	0.4720649903202059
1107	12	0.5732202404393192
1107	13	0.43388685013483297
1107	14	1.306651305619262
1107	15	0.7707335443033969
1107	16	-5.904785027319814
1107	17	-1.194611408818588
1107	18	0.5862963724605947
1107	19	0.5881202889179775
1107	20	0.5116706082341551
1107	21	0.5984489978421053
1107	22	0.6430230796663636
1107	23	2.479517214189194
1107	24	1.5184910605257755
1107	25	1.194070679245236
1108	1	0.10733096792100483
1108	2	0.4239946275729629
1108	3	0.18289750496883528
1108	4	0.6149949822765564
1108	5	0.4957550014595995
1108	6	0.14643409762274473
1108	7	0.03066236234455033
1108	8	0.09899638764535723
1108	9	0.18741482246152685
1108	10	0.5385881215800126
1108	11	0.10562909149282262
1108	12	0.2543587812526624
1108	13	0.13239618378516127
1108	14	0.6642339974283402
1108	15	0.3383555631214463
1108	16	0.5341540952800159
1108	17	0.6855024805799559
1108	18	0.5399844155165852
1108	19	0.5870707862446309
1108	20	0.3846603266181461
1108	21	0.38188147779096937
1108	22	0.7843960535566126
1108	23	2.3774184887960317
1108	24	1.2057371103816195
1108	25	1.3871967220720904
1109	1	0.2768596342005729
1109	2	0.5855985663026395
1109	3	0.413603675670398
1109	4	0.45041325476372407
1109	5	0.1990168828303993
1109	6	0.21623922912232274
1109	7	0.1457013290618363
1109	8	0.03193377823694732
1109	9	0.18197017109937264
1109	10	0.6870988780483671
1109	11	0.29946639329540403
1109	12	0.3561976751339557
1109	13	0.1829848607967577
1109	14	-1.0154047446575944
1109	15	-0.22560362079318708
1109	16	-0.44525394579174815
1109	17	0.6358471355409421
1109	18	1.4140501301230515
1109	19	0.7100138543908656
1109	20	0.4809021292121969
1109	21	0.5539870126278998
1109	22	0.26189569501661936
1109	23	-3.863309720700901
1109	24	-1.4243145032632043
1109	25	-0.1591814868608452
1110	1	0.5305548614564337
1110	2	0.8248229499751929
1110	3	0.5815005844390377
1110	4	1.0730431887655882
1110	5	0.7995035118505243
1110	6	0.48126000455966533
1110	7	0.4154135156864882
1110	8	0.4060754786695142
1110	9	0.37223022072036827
1110	10	0.7807649623114159
1110	11	0.4055938444048266
1110	12	0.500149424950628
1110	13	0.3097844642920222
1110	14	1.098281354355933
1110	15	0.48536790530258145
1110	16	1.0455855419106541
1110	17	1.2617641843496656
1110	18	1.949392062815157
1110	19	1.022043709112836
1110	20	0.7612749868521576
1110	21	0.5871126354567195
1110	22	0.836470960638465
1110	23	2.156835899506312
1110	24	1.2690984749495726
1110	25	1.5997451076467328
1111	1	0.618037598150817
1111	2	0.9853436144572107
1111	3	0.7372817881624982
1111	4	1.0797867322096693
1111	5	0.9406021215506684
1111	6	0.48420928308352984
1111	7	0.43129979070833
1111	8	0.1001884924154055
1111	9	0.4232550851881598
1111	10	0.7711827528842932
1111	11	0.4537338658691752
1111	12	0.5866606395973406
1111	13	0.43238286115325764
1111	14	-0.12173802937723366
1111	15	0.41390095159530915
1111	16	0.7444080809673892
1111	17	1.2564534023869385
1111	18	1.9235816561070749
1111	19	1.0143377728710945
1111	20	0.6898864810025316
1111	21	0.5856231877020335
1111	22	0.869340612994506
1111	23	0.7254886026666227
1111	24	0.7339025623058788
1111	25	0.2667409580841422
1112	1	0.4551637625047036
1112	2	0.7767818702519023
1112	3	0.5170854248968269
1112	4	1.050257698225188
1112	5	1.0093235303710912
1112	6	0.27833303908847284
1112	7	0.3057623721016043
1112	8	0.2719592023049273
1112	9	0.3131935384929534
1112	10	0.6898398116403612
1112	11	0.36228732739293434
1112	12	0.33456257264779055
1112	13	0.2020733437150284
1112	14	0.5305033916760706
1112	15	0.21936369864221916
1112	16	0.6946839579759398
1112	17	0.9545451999836843
1112	18	0.9514031834335483
1112	19	0.5256589242912568
1112	20	0.37879405901902174
1112	21	0.45716007112010715
1112	22	0.5034455099706889
1112	23	2.0337929466145326
1112	24	0.9874991574866314
1112	25	0.9935226903114156
1113	1	0.6022512335221357
1113	2	1.0674325821088395
1113	3	0.7598216296952884
1113	4	0.9551986313762413
1113	5	1.0407536542136668
1113	6	0.5148445645105042
1113	7	0.5195335362618749
1113	8	0.45347020190931175
1113	9	0.5300933176495377
1113	10	0.913746736909161
1113	11	0.657242232270022
1113	12	0.6813756169316534
1113	13	0.5123933913891605
1113	14	1.255756159730396
1113	15	0.7945355909347285
1113	16	1.0757239752027274
1113	17	1.5130887351809221
1113	18	2.107065237928289
1113	19	1.2583933262863596
1113	20	1.0917652291886384
1113	21	0.9466086636815376
1113	22	1.1915041912200266
1113	23	0.8690805688671333
1113	24	0.926599458454632
1113	25	1.3353216877524403
1114	1	-0.1587576896520453
1114	2	-2.8098490399084213
1114	3	-0.89200773419633
1114	4	0.35285586847042083
1114	5	0.6055181412715811
1114	6	0.3175705636201158
1114	7	0.34659117763629155
1114	8	0.3083053415922379
1114	9	0.44259540318893187
1114	10	0.6408391302867429
1114	11	0.4841491698430147
1114	12	0.5616687997540168
1114	13	0.4815710055656339
1114	14	1.2680264674344386
1114	15	0.6265714882029485
1114	16	1.0416967755769946
1114	17	1.312976514297354
1114	18	1.6138259487974906
1114	19	0.8968146630940552
1114	20	0.8093484013380703
1114	21	0.6031473641662259
1114	22	0.764510226917757
1114	23	2.3936612182475088
1114	24	1.3608372661188568
1114	25	1.4326187029368427
1115	1	0.1719147586386569
1115	2	0.4675467221333065
1115	3	0.14261500512768066
1115	4	0.3774519345771168
1115	5	0.44741353726618627
1115	6	0.015356511420463903
1115	7	-0.24622693356057113
1115	8	-0.1131330748049848
1115	9	-0.10095175273363566
1115	10	-0.014660091929080465
1115	11	-0.03464321865612163
1115	12	-0.04722932138700031
1115	13	-0.13561007917896717
1115	14	0.6849350375376844
1115	15	-0.01678339010036467
1115	16	0.44202770580940864
1115	17	0.8404505190427756
1115	18	1.4415650607158952
1115	19	0.5841919571142
1115	20	0.29750309966733823
1115	21	0.3562741705280388
1115	22	0.5459118254536506
1115	23	2.291604136844917
1115	24	1.0187911882889438
1115	25	0.7677481890716891
1116	1	0.7018172756780388
1116	2	0.7646191411288186
1116	3	0.7647268674721914
1116	4	1.4457809748057358
1116	5	0.9380636298160354
1116	6	0.7190175877025382
1116	7	0.5863513311046589
1116	8	0.5081848867684968
1116	9	0.6324676584278635
1116	10	1.2094665812671366
1116	11	0.8102516542118892
1116	12	0.7990056185886374
1116	13	0.6640529444611545
1116	14	1.5109999360492665
1116	15	0.6989091660731432
1116	16	1.2545366048329925
1116	17	1.5991018081966857
1116	18	2.113293204354406
1116	19	1.2646627172598102
1116	20	0.9591352561003106
1116	21	0.8515621824825672
1116	22	1.3134923409426307
1116	23	2.6081073491227684
1116	24	1.5682499838321675
1116	25	1.8619990962327577
1201	1	0.9490465706076954
1201	2	1.2363967931695754
1201	3	0.9815738320457861
1201	4	1.52473446857352
1201	5	1.3180296808203593
1201	6	0.8938650192993549
1201	7	0.7799751169565496
1201	8	0.7528840983674314
1201	9	0.9435257055223687
1201	10	1.2694393981206815
1201	11	0.9920550374358754
1201	12	1.1896181524740115
1201	13	1.015124927408813
1201	14	1.9003375115101875
1201	15	1.3045937517621766
1201	16	1.660960194894379
1201	17	2.160209251978017
1201	18	2.7137799114066112
1201	19	1.8107595557221643
1201	20	1.5354441560360925
1201	21	1.486818169585547
1201	22	1.6706011977685762
1201	23	3.316433385218275
1201	24	2.1066884647493325
1201	25	2.309184682578916
1202	1	1.162145603700612
1202	2	1.3620120251555647
1202	3	1.1325205816480413
1202	4	1.7846594678844525
1202	5	1.5701683464330074
1202	6	1.0880633709493992
1202	7	0.8426050040300623
1202	8	0.9809635978626687
1202	9	1.0763469645544887
1202	10	1.567651255338717
1202	11	1.1893910936585996
1202	12	1.2447258974863118
1202	13	1.1988014754145553
1202	14	2.028190709710074
1202	15	1.3795892942431038
1202	16	1.6190985153423654
1202	17	2.098704180813826
1202	18	2.7456147749356177
1202	19	1.9030872132813101
1202	20	1.6826483051485965
1202	21	1.5389219070051325
1202	22	1.886639963259384
1202	23	3.408059505330769
1202	24	2.2283466549817206
1202	25	2.3896408717712347
1203	1	0.873579508359267
1203	2	1.2139375499486462
1203	3	0.9350065187314307
1203	4	1.5691945868402541
1203	5	1.3600793793669652
1203	6	0.8480641969021008
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2211	15	-0.03968941546708038
2211	16	-0.2591936756769755
2211	17	0.4075168714946855
2211	18	-5.114653965207868
2211	19	-1.6474319032939562
2211	20	-0.8000398310508245
2211	21	-0.5373318525853037
2211	22	-0.019946822972208622
2211	23	-4.389069402090513
2211	24	-1.8094684361101763
2211	25	-0.9428437724595398
2212	1	0.05988350687961841
2212	2	0.3624429007405183
2212	3	0.1854584777526398
2212	4	0.764239413931044
2212	5	0.675601877683917
2212	6	0.14247230150359513
2212	7	-0.06732154925818179
2212	8	-0.15462625014259856
2212	9	-0.3394625658872883
2212	10	-3.7489899047755593
2212	11	-1.2059614632696452
2212	12	-0.4867838969745435
2212	13	-0.1831660829037034
2212	14	0.4516967525246045
2212	15	0.1387574766566857
2212	16	0.30452286091828995
2212	17	0.8730802374842455
2212	18	1.446940839316035
2212	19	0.6877611743245424
2212	20	0.18621872506437853
2212	21	0.11088128001372866
2212	22	0.3452530810886003
2212	23	1.6912464592404604
2212	24	0.9113042433606822
2212	25	0.6634845240663552
2213	1	-1.5168529177486363
2213	2	-3.8618439945920606
2213	3	-1.7756797466386445
2213	4	-3.577666795708584
2213	5	-1.263772319870931
2213	6	-1.0381714833325226
2213	7	-0.9462733892149018
2213	8	-0.8692548490019334
2213	9	-0.8795804419840465
2213	10	-0.8472674812673944
2213	11	-1.0252620549360287
2213	12	-0.8423239592271372
2213	13	-1.2452327625237913
2213	14	-6.079099996929639
2213	15	-2.7271540819822917
2213	16	-2.0268026927450506
2213	17	-0.8638465261806587
2213	18	-0.17289770014807715
2213	19	-0.7844792715778013
2213	20	-0.9868412149689192
2213	21	-1.2051036178257206
2213	22	-1.0461824242280144
2213	23	-5.749829588748142
2213	24	-3.0846908648194002
2213	25	-2.2115950061055827
2214	1	-0.003389440521843312
2214	2	0.20997077158124355
2214	3	0.1628801607435797
2214	4	0.4443139781722394
2214	5	0.17823575487499
2214	6	-0.01256356065078279
2214	7	-0.1451391145606138
2214	8	-0.17633732011383785
2214	9	-0.10470029812650239
2214	10	0.41807082920818317
2214	11	-0.13104126964177
2214	12	-0.1276951522887373
2214	13	-0.24882521414061642
2214	14	0.3708499615414951
2214	15	-0.007628015345595429
2214	16	0.47768240319805244
2214	17	0.6793168785915142
2214	18	1.18257430439661
2214	19	0.39226182665488807
2214	20	0.23111442694407458
2214	21	0.1880701098975024
2214	22	0.5564246920135858
2214	23	2.0125649565959023
2214	24	0.9488928844850406
2214	25	0.8635305395130217
2215	1	-2.4002165972109175
2215	2	-4.625543659241259
2215	3	-2.3796389212494837
2215	4	-3.8742636439262093
2215	5	-4.848731262654802
2215	6	-2.3005367059662447
2215	7	-1.515550270020288
2215	8	-1.8767867773659113
2215	9	-1.577535340675233
2215	10	-2.9639438233571447
2215	11	-1.7777769730759871
2215	12	-1.542490367988961
2215	13	-1.5566796289107714
2215	14	-0.631882569842837
2215	15	-1.315304743540679
2215	16	-2.1136917174517578
2215	17	-6.630919496752686
2215	18	-2.852872810148803
2215	19	-1.9487913724133747
2215	20	-1.6565947567166148
2215	21	-1.6532653832359827
2215	22	-1.3892144910708168
2215	23	-6.339282440911724
2215	24	-3.4820083351422504
2215	25	-3.1412001828507634
2216	1	-0.7967129123184691
2216	2	-0.32280797688881435
2216	3	-0.5726266631197063
2216	4	-2.9250252335385234
2216	5	-1.1086046303227093
2216	6	-0.9291163901017256
2216	7	-0.8434667364342125
2216	8	-0.7032655379914061
2216	9	-0.7547983418576294
2216	10	-0.36878009042339244
2216	11	-0.9653473640322616
2216	12	-1.7880914625910194
2216	13	-0.9967732044156179
2216	14	-6.678104532643985
2216	15	-2.931055910330521
2216	16	-1.3594505942746622
2216	17	-0.5297409216423006
2216	18	0.1875254883199844
2216	19	-0.27847611288824226
2216	20	-0.7875491600747608
2216	21	-0.9396843995751567
2216	22	-0.383427610323686
2216	23	0.6726756122492716
2216	24	0.00426848360034171
2216	25	0.03329098158686605
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94718.0
3	1.0
4	834.0
5	4820.0
6	5824.0
7	5182.0
8	4871.0
9	4539.0
10	4317.0
11	4132.0
12	4104.0
13	4016.0
14	3988.0
15	4180.0
16	4286.0
17	4647.0
18	5148.0
19	5990.0
20	7486.0
21	9396.0
22	11458.0
23	13939.0
24	16812.0
25	19534.0
26	22332.0
27	25813.0
28	28854.0
29	34533.0
30	40746.0
31	46449.0
32	59734.0
33	78450.0
34	102219.0
35	135983.0
36	192625.0
37	270082.0
38	641602.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.41748179110194	18.175852472017937	12.335318496993112	25.07134723988701
2	14.079885409889014	19.978269903608457	41.85644093475822	24.085403751744312
3	19.021441238110594	24.984372806082384	29.088578367387267	26.905607588419755
4	10.623980763470563	16.509031683695543	39.42734507604753	33.43964247678637
5	12.222524425131194	38.21917430653533	36.27031223916581	13.287989029167665
6	32.01152616681121	36.54582077663321	18.47493547467966	12.967717581875924
7	28.212646191266767	31.56088310886099	21.961980001570257	18.264490698301987
8	25.722651253434385	36.063510947402136	20.429898980655118	17.783938818508357
9	26.835419473565707	15.068309371017149	19.75462394096165	38.34164721445549
10	13.974511050983619	29.30371080698364	35.09304189238311	21.628736249649627
11	34.43335376763238	22.825943968424067	23.791763573600132	18.948938690343418
12	23.08990189533145	25.19191452810136	31.556967053719433	20.16121652284776
13	28.720922977736645	21.745349236224484	25.60067382782035	23.933053958218515
14	20.877651277230317	21.7288261793738	28.53214186179041	28.86138068160547
15	23.9275864794263	29.60490466048818	23.468931465366634	22.99857739471889
16	22.221372950308233	28.13131754311528	27.77540328283459	21.8719062237419
17	21.810447300193747	28.07016449157575	28.13745035457674	21.98193785365377
18	21.500079552509334	27.17789292960175	29.918294893352844	21.403732624536072
19	23.048517940717627	27.25579823192928	28.170982839313574	21.524700988039523
20	23.467835278188264	27.055980831886956	28.141986715848738	21.334197174076042
21	23.123170594098998	26.70598518951462	27.753774250596823	22.41706996578956
22	22.884030145380947	27.467617429072444	27.37884747793492	22.269504947611686
23	21.316282373545185	26.94017673566298	29.445873273228923	22.29766761756291
24	22.493465510480664	27.381669313745988	27.95398970499367	22.170875470779677
25	22.544836584177443	26.79560840939783	27.945559086425188	22.71399591999954
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	222.0
1	222.0
2	392.5
3	563.0
4	563.0
5	563.0
6	1254.5
7	1946.0
8	1946.0
9	1946.0
10	3005.0
11	4064.0
12	4064.0
13	4064.0
14	6770.0
15	9476.0
16	9476.0
17	9476.0
18	16329.5
19	23183.0
20	23183.0
21	23183.0
22	36129.5
23	49076.0
24	49076.0
25	49076.0
26	68109.5
27	87143.0
28	87143.0
29	87143.0
30	110545.0
31	133947.0
32	133947.0
33	133947.0
34	154512.0
35	175077.0
36	175077.0
37	175077.0
38	192768.5
39	210460.0
40	210460.0
41	210460.0
42	225120.5
43	239781.0
44	239781.0
45	239781.0
46	254120.5
47	268460.0
48	268460.0
49	268460.0
50	256272.0
51	244084.0
52	244084.0
53	244084.0
54	215614.5
55	187145.0
56	187145.0
57	187145.0
58	159135.0
59	131125.0
60	131125.0
61	131125.0
62	106342.0
63	81559.0
64	81559.0
65	81559.0
66	62643.0
67	43727.0
68	43727.0
69	43727.0
70	31937.5
71	20148.0
72	20148.0
73	20148.0
74	14059.5
75	7971.0
76	7971.0
77	7971.0
78	5478.0
79	2985.0
80	2985.0
81	2985.0
82	2004.0
83	1023.0
84	1023.0
85	1023.0
86	677.0
87	331.0
88	331.0
89	331.0
90	218.0
91	105.0
92	105.0
93	105.0
94	67.0
95	29.0
96	29.0
97	29.0
98	21.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10688048308314843
2	0.05016520728367619
3	0.03711705492284435
4	0.03306225060354202
5	0.03779285564272807
6	0.019130358839785325
7	0.020429975608792478
8	0.02328913250060822
9	0.028851492271958844
10	0.03602537683687834
11	0.021157760999436486
12	0.020377990938032192
13	0.015803339911127007
14	0.021833561719320205
15	0.021417684353237917
16	0.022249439085402497
17	0.02630424340470482
18	0.020066082913470475
19	0.03347812796962432
20	0.023393101842128793
21	0.028435614905876552
22	0.036181330849159206
23	0.02838363023511627
24	0.04002819648542038
25	0.03145072580997315
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1923644.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.94961131095097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.41411351820793	66.49532447006494
2	9.578297514229474	14.740925414812118
3	2.2326807479839896	5.154117472164339
4	0.7609817541300279	2.342290007801252
5	0.3355844861312096	1.29115478848909
6	0.18653057412971646	0.8612073106134127
7	0.11382184870773998	0.6130982911728121
8	0.07402623329733832	0.45570319032351686
9	0.053157998791021274	0.36814386105333796
>10	0.22629649263093965	2.9789067912258176
>50	0.01228146518386782	0.669736310531001
>100	0.010110668776069563	1.6485203771418906
>500	8.193668906104441E-4	0.438722664451686
>1k	0.001229050335915634	1.6298415630288672
>5k	6.828057421753522E-5	0.31230748712584094
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	5944	0.30899688299914124	No Hit
CCCATGTACTCTGCGTTGATACCAC	4610	0.2396493322049194	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3673	0.19093969570253125	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2452	0.1274664127042218	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2110	0.1096876553042039	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2024	0.10521697361881928	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.1984670760286204E-5
2	0.0	0.0	0.0	0.0	5.1984670760286204E-5
3	0.0	0.0	0.0	0.0	5.1984670760286204E-5
4	0.0	0.0	0.0	0.0	1.0396934152057241E-4
5	0.0	0.0	0.0	0.0	1.0396934152057241E-4
6	0.0	0.0	0.0	0.0	1.0396934152057241E-4
7	0.0	0.0	0.0	0.0	1.0396934152057241E-4
8	0.0	0.0	0.0	0.0	1.0396934152057241E-4
9	0.0	0.0	0.0	0.0	1.0396934152057241E-4
10	0.0	0.0	0.0	5.1984670760286204E-5	1.0396934152057241E-4
11	0.0	0.0	0.0	5.1984670760286204E-5	1.0396934152057241E-4
12	0.0	0.0	0.0	5.1984670760286204E-5	1.0396934152057241E-4
13	0.0	0.0	0.0	5.1984670760286204E-5	1.0396934152057241E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTGG	360	0.0	12.401199	7
GTATACG	55	0.0030438213	12.104163	1
ACCGATG	55	0.0030718283	12.08937	8
CTGATCG	55	0.0030718283	12.08937	9
GTCTACG	65	7.951518E-4	11.705125	1
GTAGGAC	1015	0.0	11.606849	3
TGTAGGA	1175	0.0	11.320652	2
GGACGTG	815	0.0	11.305604	6
GTCCTAC	985	0.0	10.910424	1
TAGTACA	245	0.0	10.859432	4
CGTGCGC	105	3.4722962E-6	10.855761	10
GTATTAT	285	0.0	10.67836	1
AGGACGT	855	0.0	10.668083	5
GTTCTAA	215	0.0	10.616276	1
TAGGACC	495	0.0	10.55778	4
CTGTAGG	1225	0.0	10.4808655	1
GACGTGA	445	0.0	10.459343	7
ATAGTAC	300	0.0	10.450844	3
GGCGAGG	375	0.0	10.386695	19
TAGGACG	915	0.0	10.384703	4
>>END_MODULE