FastQCFastQC Report
Thu 26 May 2016
SRR1513050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923644
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC67860.3527679757793022No Hit
CCCATGTACTCTGCGTTGATACCAC63840.3318701381336671No Hit
GTATCAACGCAGAGTACTTTTTTTT58240.30275872250790686No Hit
TATCAACGCAGAGTACTTTTTTTTT39520.2054434188446511No Hit
GGTATCAACGCAGAGTACTTTTTTT33910.17628001854813052No Hit
GAGTACATGGGAAGCAGTGGTATCA24850.12918190683931122No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19500.10137010798255812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGCT551.9841988E-413.7986477
TGAACCG801.9563158E-613.0882755
TAGGACC4950.011.730174
TGGACCG907.3010124E-611.6340225
ACACCGC600.0057909111.1052046
ATTATAC1803.6379788E-1211.1052033
ACCGTCC600.00593871711.0676658
TGGCGAG8700.010.58165718
TGTAGGA9900.010.3927622
TTACACA2950.010.3254194
GTCTTAT1855.638867E-1110.3010171
GTAGGAC8900.010.267413
TTAGGAC6450.010.1828443
CGTGCGC856.69196E-410.04277510
TAGGACA5450.09.9553854
GGCGAGA5450.09.92328219
CTATACT2700.09.8712924
AGGACCT8200.09.8669935
GGTCTAC1659.731593E-99.8171811
ATGGCGA9350.09.74706517
Produced by FastQC (version 0.11.5)