FastQCFastQC Report
Thu 26 May 2016
SRR1513049_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513049_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645180
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC58860.3577724018040579No Hit
CCCATGTACTCTGCGTTGATACCAC43960.2672048043375193No Hit
GAGTACATGGGAAGCAGTGGTATCA24020.14600226115075554No Hit
GTATCAACGCAGAGTACTTTTTTTT20750.12612601660608566No Hit
GTCCTACAGTGGACATTTCTAAATT17200.10454783063251437No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG250.00603116619.00057618
TCCAACG1150.016.52224318
TACGACA400.00527714514.2508674
TAGGACC5300.013.623474
CCAACGA500.001499268813.30040419
CCAACGT957.384733E-813.00039519
GCCGGTT957.405288E-812.99762811
GTCCTAC9900.012.3931091
CTACGAG550.003068576312.0909093
AATGACG802.871061E-511.8731958
GTCTAAC1302.568413E-911.7058351
GACGAAA658.0361444E-411.69017511
AGGACCT8150.011.423665
GCGTTTA1001.9317322E-611.39895913
GGACCTG7850.011.3744136
TCCTACA11500.011.3180792
TGTAGGA10250.011.3080052
GTAGGAC9850.011.2842653
CCTACAG10700.010.9205613
TGACGAA700.001493375410.85549310
Produced by FastQC (version 0.11.5)