##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513049_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1645180
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.123439988329544	31.0	30.0	34.0	23.0	34.0
2	28.830315831702308	31.0	30.0	34.0	16.0	34.0
3	29.173702573578574	31.0	30.0	34.0	22.0	34.0
4	32.04642470732686	35.0	32.0	37.0	19.0	37.0
5	32.27164991064807	35.0	33.0	37.0	19.0	37.0
6	32.75823435733476	36.0	33.0	37.0	26.0	37.0
7	32.740876986104865	36.0	33.0	37.0	26.0	37.0
8	32.71251230868355	37.0	35.0	37.0	25.0	37.0
9	34.07629499507652	39.0	34.0	39.0	25.0	39.0
10	33.58143668170048	38.0	34.0	39.0	18.0	39.0
11	33.9520970349749	39.0	34.0	39.0	25.0	39.0
12	33.788841342588654	39.0	34.0	39.0	22.0	39.0
13	33.87772888073037	39.0	34.0	39.0	23.0	39.0
14	34.1625481710208	39.0	33.0	41.0	16.0	41.0
15	34.71054717416939	40.0	34.0	41.0	19.0	41.0
16	34.301431454308954	39.0	34.0	41.0	17.0	41.0
17	33.854999452947396	39.0	33.0	41.0	11.0	41.0
18	33.2773909237895	39.0	32.0	41.0	10.0	41.0
19	34.169713344436474	39.0	33.0	41.0	18.0	41.0
20	34.42458636744915	39.0	34.0	41.0	18.0	41.0
21	34.44451792509027	39.0	34.0	41.0	18.0	41.0
22	34.033111878335504	39.0	34.0	41.0	10.0	41.0
23	32.38924373016934	39.0	31.0	41.0	2.0	41.0
24	33.46149053598998	39.0	33.0	41.0	2.0	41.0
25	33.1956624807012	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7055344971139661
1101	2	1.135170595705727
1101	3	0.9173467142137852
1101	4	0.9178360178957661
1101	5	1.133960443260392
1101	6	0.9650867190563943
1101	7	0.9147026146869948
1101	8	0.8308307929308612
1101	9	1.0320727305447974
1101	10	1.3200788874209337
1101	11	0.997155692803176
1101	12	1.022194651775905
1101	13	0.9007577681995187
1101	14	0.21154271852346795
1101	15	0.6950163107080414
1101	16	1.3197916713760023
1101	17	1.8760237554687933
1101	18	-4.269302429190972
1101	19	-0.609216202968291
1101	20	0.4635925272824153
1101	21	0.7671002262651285
1101	22	1.3816079277821203
1101	23	3.103796307751942
1101	24	2.1012657306094553
1101	25	2.2234595320878228
1102	1	0.8984541778421189
1102	2	1.1702833513053221
1102	3	1.030042261039931
1102	4	1.5085700389638461
1102	5	1.2765585153054317
1102	6	0.8235255363823981
1102	7	0.760640665034714
1102	8	0.7651379602287349
1102	9	0.841201534593921
1102	10	1.0799637688754657
1102	11	0.6973613408965704
1102	12	0.9068631474410083
1102	13	0.7669598744850887
1102	14	1.5025078448366287
1102	15	1.076854166928662
1102	16	1.3314959232425352
1102	17	1.2239879339926176
1102	18	1.5116972013539538
1102	19	1.2656973079906848
1102	20	1.1272408896042663
1102	21	1.0847207854608385
1102	22	1.494202135562091
1102	23	3.062168153186562
1102	24	1.9358924830732462
1102	25	2.311872709492839
1103	1	0.6892941398261065
1103	2	1.013367916046775
1103	3	0.7833637665889377
1103	4	1.2211246902465618
1103	5	1.1181260949047243
1103	6	0.7052410024506273
1103	7	0.6235942103020946
1103	8	0.6318864161545719
1103	9	0.7762871032609979
1103	10	1.0521129921712387
1103	11	0.7726327532985096
1103	12	1.0083903480812282
1103	13	0.7911353565064587
1103	14	1.4547420689091766
1103	15	1.1647686452594002
1103	16	1.447684485017902
1103	17	1.8346108443847484
1103	18	1.7428778387749233
1103	19	1.132156107222528
1103	20	1.1140188366612236
1103	21	1.0740429790056893
1103	22	1.396224249341195
1103	23	2.8147179480280258
1103	24	1.7525040578526543
1103	25	1.8454652162128724
1104	1	0.6489043450887202
1104	2	1.0028662979793594
1104	3	0.8356347594827156
1104	4	1.2983422585457447
1104	5	1.1367905691558633
1104	6	0.7466560392358872
1104	7	0.6791629833588004
1104	8	0.6578323981623129
1104	9	0.8886534681925511
1104	10	1.0527711419707089
1104	11	0.7571594471185534
1104	12	0.8640972590138531
1104	13	0.7881072133669775
1104	14	1.5733377194955622
1104	15	1.0814078004468755
1104	16	1.2817313181400536
1104	17	1.7014719636580082
1104	18	2.405003517098443
1104	19	1.5554345631262478
1104	20	0.9867200747513678
1104	21	0.9981078626014721
1104	22	1.4136286733178594
1104	23	2.8271725949662283
1104	24	1.841757509950547
1104	25	1.8768536473946824
1105	1	0.6693901011356509
1105	2	1.014201251414221
1105	3	0.7126872938708644
1105	4	0.8243190611239228
1105	5	0.8748997314998803
1105	6	0.49431260891975626
1105	7	0.36674596244625235
1105	8	0.29596585395831454
1105	9	0.5895973616177486
1105	10	0.9541629711385085
1105	11	0.6140466407567402
1105	12	0.7516177692247723
1105	13	0.6912434899475244
1105	14	1.316368041113428
1105	15	0.7277442106817631
1105	16	1.060630881261467
1105	17	1.5483045737029073
1105	18	1.7142176763876265
1105	19	1.1921964477493034
1105	20	0.9319216337291678
1105	21	0.8137323455585275
1105	22	1.3017730652027169
1105	23	2.752545531737873
1105	24	1.657268031597951
1105	25	1.780014298136365
1106	1	-0.33026425044214847
1106	2	0.08060060790588963
1106	3	-0.10020185723657704
1106	4	-2.328737235217229
1106	5	-0.29140462077346996
1106	6	-0.20038720311755043
1106	7	-0.24712203487955264
1106	8	-0.07807549128832392
1106	9	-0.06830414985763866
1106	10	0.06573327385091687
1106	11	-0.06411932776666873
1106	12	0.04844580667983678
1106	13	-0.06016590295015334
1106	14	0.7484002558082352
1106	15	0.1248705171943385
1106	16	-0.8004808072032574
1106	17	-5.673266375463701
1106	18	-6.393643452157747
1106	19	-2.5122294722818594
1106	20	-1.2389995471380786
1106	21	-0.7772911059995522
1106	22	-1.2163321529919386
1106	23	-4.685668912667474
1106	24	-1.692261734344715
1106	25	-0.31033561361036277
1107	1	0.4873666607929721
1107	2	0.7673313004054663
1107	3	0.5286169081210268
1107	4	0.7446294732044834
1107	5	0.8357735074093213
1107	6	0.41668938294832003
1107	7	0.5103660107350336
1107	8	0.3464239185711975
1107	9	0.3106004548463446
1107	10	0.8434520652779511
1107	11	0.49945558388916567
1107	12	0.5280189370586683
1107	13	0.46298579298605347
1107	14	1.1187032353301376
1107	15	0.6601830150332759
1107	16	-5.456228280149187
1107	17	-1.002497700400248
1107	18	0.44843932961925503
1107	19	0.5364103580433053
1107	20	0.4173214210943712
1107	21	0.49866755666604945
1107	22	0.5836278342129759
1107	23	2.585381428028292
1107	24	1.5353091078426004
1107	25	1.3193377658674095
1108	1	0.5554424112081868
1108	2	0.7603229693661255
1108	3	0.6740323466789704
1108	4	1.07244280762918
1108	5	0.8556089967312488
1108	6	0.5422358754449021
1108	7	0.5302408700805685
1108	8	0.41507139019871886
1108	9	0.7106289942231356
1108	10	1.0961889432724305
1108	11	0.7258414670747229
1108	12	0.7161141266175477
1108	13	0.5503751075881738
1108	14	1.2744213266651698
1108	15	0.8445201568847196
1108	16	1.1949741628463642
1108	17	1.057216510575465
1108	18	0.7645283332604365
1108	19	0.9824924882696919
1108	20	0.8623074151695675
1108	21	0.8621664718118254
1108	22	1.25783295265839
1108	23	2.88138907421596
1108	24	1.6595350706147443
1108	25	1.7668351939778262
1109	1	0.30888549817973754
1109	2	0.6236545329773477
1109	3	0.5302294093518256
1109	4	0.48659204459085714
1109	5	0.22477369818324888
1109	6	0.33402719434059946
1109	7	0.27909801029194625
1109	8	0.19058008241523083
1109	9	0.27071193451353537
1109	10	0.7685879147757859
1109	11	0.43521554105733884
1109	12	0.3842470903670545
1109	13	0.2737851357391037
1109	14	-0.7153579107903809
1109	15	0.0702072617839633
1109	16	-0.0014949328667910322
1109	17	1.023387552159818
1109	18	1.6692183026401324
1109	19	0.8739732826691409
1109	20	0.7505025455528198
1109	21	0.7209170274222529
1109	22	0.5937022360444004
1109	23	-4.18317825959479
1109	24	-1.398711114193631
1109	25	-0.12914542756504233
1110	1	0.658106053842296
1110	2	0.9015797063209519
1110	3	0.64222201817309
1110	4	1.209303668242626
1110	5	0.9387874452941887
1110	6	0.6002306082466049
1110	7	0.5491137797449497
1110	8	0.5500754599190856
1110	9	0.6519950383270938
1110	10	0.9789686688204711
1110	11	0.5986942101636572
1110	12	0.5769919382098934
1110	13	0.5262853764941156
1110	14	1.3100561878228731
1110	15	0.5814673734942915
1110	16	1.2389747801532351
1110	17	1.5541861365203715
1110	18	2.2023293388496796
1110	19	1.2914213999649817
1110	20	0.911264294397192
1110	21	0.8245102959122406
1110	22	1.1477384937485624
1110	23	2.42652476910321
1110	24	1.3569719077632243
1110	25	1.746961443582876
1111	1	0.3717521216436417
1111	2	0.6787164569404815
1111	3	0.43755407813062774
1111	4	0.8884845657122042
1111	5	0.7194987605872427
1111	6	0.18386098370311998
1111	7	0.06731145595247057
1111	8	-0.010360777799824916
1111	9	0.19070341396822243
1111	10	0.41569632971434345
1111	11	0.124893680458257
1111	12	0.31951854774032995
1111	13	0.21841719219072075
1111	14	-0.4536355605599667
1111	15	-0.019119255113118072
1111	16	0.45335457750186947
1111	17	0.9863711831121975
1111	18	1.5310342588613253
1111	19	0.7353254092052381
1111	20	0.46955496490765825
1111	21	0.2847167106019768
1111	22	0.6143567579338054
1111	23	0.6170299537308637
1111	24	0.47986391460820954
1111	25	0.029973356037110932
1112	1	0.4522920948706357
1112	2	0.7327748944922661
1112	3	0.5597241372592485
1112	4	1.1368365214462486
1112	5	0.8938133046293046
1112	6	0.22981758980731826
1112	7	0.18343672729653804
1112	8	0.055298904144379435
1112	9	0.17113902183182006
1112	10	0.5842813563404405
1112	11	0.1879263052118887
1112	12	0.25381420494242235
1112	13	0.2993153337115615
1112	14	0.8290041779624886
1112	15	0.4844562575758573
1112	16	0.8354939969342752
1112	17	0.9439803935905786
1112	18	1.0393156151879523
1112	19	0.5025283587020439
1112	20	0.4222723427984221
1112	21	0.4859867242470415
1112	22	0.38249993592963705
1112	23	2.0896540256959497
1112	24	0.9144601511085853
1112	25	1.0063022221097881
1113	1	0.38216160222165385
1113	2	0.6836938293845307
1113	3	0.5235560373458377
1113	4	0.5450360939920529
1113	5	0.7006648845604957
1113	6	0.21338488520886045
1113	7	0.12365499008236469
1113	8	-0.03846183375243584
1113	9	0.30958029154946587
1113	10	0.37034923588357316
1113	11	0.18687628207530338
1113	12	0.24133447809160913
1113	13	0.06706264322413347
1113	14	0.8938112508862659
1113	15	0.30646287555642004
1113	16	0.8702724698278175
1113	17	1.3086373650340661
1113	18	1.7900921798856402
1113	19	0.7484937729386019
1113	20	0.5772428150138964
1113	21	0.45889887622465153
1113	22	0.7861002525114671
1113	23	0.8214414073213803
1113	24	0.7093680399979831
1113	25	1.0518449354589094
1114	1	-0.3941500258195205
1114	2	-2.973803074509682
1114	3	-1.0285267873679658
1114	4	-0.0074365182334616975
1114	5	0.2487008548733911
1114	6	-0.06549878555191668
1114	7	-0.025133422713985
1114	8	-0.08568937644318453
1114	9	-0.1204635311091451
1114	10	0.1373434165363463
1114	11	0.04165144265110854
1114	12	0.07138629070063729
1114	13	0.03887559626542014
1114	14	0.7995559142652198
1114	15	0.2737110145638013
1114	16	0.6149472233600406
1114	17	0.88303184886108
1114	18	1.0738386232479868
1114	19	0.44933726756536174
1114	20	0.27155286060100536
1114	21	0.06897403724434525
1114	22	0.31395161058109977
1114	23	1.9668185551754078
1114	24	0.7409183567107362
1114	25	0.6830357598886252
1115	1	-0.15991449504151944
1115	2	0.15659130991154768
1115	3	-0.05112594359466627
1115	4	0.036391506810666385
1115	5	0.06782238084774406
1115	6	-0.3486353259956303
1115	7	-0.4904854925099329
1115	8	-0.5813378496165811
1115	9	-0.6422284012601409
1115	10	-0.36708154247294544
1115	11	-0.40004126028033227
1115	12	-0.47851784546085696
1115	13	-0.7681419475942306
1115	14	0.13692426563767413
1115	15	-0.4616313589680203
1115	16	-0.06581333037221526
1115	17	0.5942112822755092
1115	18	0.8588321403118542
1115	19	0.0742949904601744
1115	20	-0.42432346673930255
1115	21	-0.2961433711677657
1115	22	-0.06409388199608657
1115	23	1.6065155107414384
1115	24	0.4457207142590036
1115	25	0.2242623024602679
1116	1	0.13716495004335982
1116	2	0.18070161731262502
1116	3	0.2746969194428601
1116	4	0.9080293191528597
1116	5	0.33286437256490586
1116	6	0.20619298251476437
1116	7	0.12283526231015429
1116	8	0.15998492611155513
1116	9	0.313878076947411
1116	10	0.648679727082552
1116	11	0.15758194220814659
1116	12	0.20520718784214864
1116	13	0.05832089449898348
1116	14	0.8503433087218184
1116	15	0.05062797202099745
1116	16	0.5810467731620648
1116	17	0.9388093895868224
1116	18	1.3302706686655483
1116	19	0.5932378985657394
1116	20	0.40991189800902106
1116	21	0.25210878927385494
1116	22	0.6565540803418983
1116	23	1.8649816027806807
1116	24	0.9151305102481402
1116	25	1.1628130113305275
1201	1	1.1321176180250987
1201	2	1.4464992494721933
1201	3	1.2363126889910028
1201	4	1.7130466193331202
1201	5	1.5776917072038685
1201	6	1.168876582227199
1201	7	1.086787282199161
1201	8	1.09926564942554
1201	9	1.3450249177609592
1201	10	1.689389300085061
1201	11	1.174817667345998
1201	12	1.3468775671711768
1201	13	1.317974890893744
1201	14	2.077258068591064
1201	15	1.6080970813715965
1201	16	1.947279229504339
1201	17	2.410985812469093
1201	18	2.9312447201658998
1201	19	2.03495991282378
1201	20	1.8587435249342619
1201	21	1.6146364217521523
1201	22	2.028632970580489
1201	23	3.5733334994258286
1201	24	2.550124259517709
1201	25	2.5662564732081563
1202	1	0.9583982566768725
1202	2	1.2582406816259954
1202	3	1.016460278004569
1202	4	1.5858184727235312
1202	5	1.451905339768011
1202	6	1.0666966891194676
1202	7	0.8633255261600894
1202	8	0.960696790031939
1202	9	1.0489709819132074
1202	10	1.6217420276955252
1202	11	1.2501885396240908
1202	12	1.282404978368696
1202	13	1.0868949074426268
1202	14	2.0539449147624467
1202	15	1.3940619403261465
1202	16	1.6638453363797296
1202	17	2.126712823453836
1202	18	2.7150273218866516
1202	19	1.9639945894873208
1202	20	1.67122070689598
1202	21	1.5409243216482977
1202	22	1.884870463931378
1202	23	3.4966708011136447
1202	24	2.270379922866155
1202	25	2.4769826820786918
1203	1	1.1454086958363092
1203	2	1.4047209728054249
1203	3	1.2120216153997205
1203	4	1.7832486271737409
1203	5	1.5604740729785789
1203	6	1.2415350931842326
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2211	14	-0.4571073323098531
2211	15	-0.5071952401443056
2211	16	-0.6396279472323911
2211	17	-0.11710337292695527
2211	18	-5.3292866867543935
2211	19	-2.0508355475950353
2211	20	-1.359385855912663
2211	21	-1.101064931216385
2211	22	-0.8832209104075801
2211	23	-5.416669720981215
2211	24	-2.7596416844790177
2211	25	-2.0575067910918428
2212	1	0.2554513347995915
2212	2	0.4881991895760365
2212	3	0.2610722515507504
2212	4	0.777251011982159
2212	5	0.7280293073117576
2212	6	0.23770273661647678
2212	7	0.1520456884854795
2212	8	0.03107161762108035
2212	9	-0.17344792865498704
2212	10	-3.5915528545104785
2212	11	-0.9683666660415469
2212	12	-0.2380514499369042
2212	13	-0.010606998254736766
2212	14	0.6864528818221629
2212	15	0.3640384228074396
2212	16	0.5495622646130087
2212	17	1.1250417340567012
2212	18	1.5992051966378185
2212	19	0.993411498088534
2212	20	0.4563954370495509
2212	21	0.5009306744980506
2212	22	0.6257214621890768
2212	23	1.9311047278523077
2212	24	0.9908230215316465
2212	25	0.9670407558228007
2213	1	-1.2956038500413776
2213	2	-3.7427415553929677
2213	3	-1.5875597188998931
2213	4	-3.1661474112239887
2213	5	-1.1593619057762012
2213	6	-0.7942834048369107
2213	7	-0.6493865320760719
2213	8	-0.5376620751976233
2213	9	-0.48303083279709824
2213	10	-0.4367591945394409
2213	11	-0.45438432983019084
2213	12	-0.4504704163276614
2213	13	-0.6972416670535466
2213	14	-5.625471721042867
2213	15	-2.4485965350790764
2213	16	-1.6530293389494233
2213	17	-0.4554075176565533
2213	18	0.2631105654557402
2213	19	-0.35898656096131276
2213	20	-0.5134583381503219
2213	21	-0.7752986621339844
2213	22	-0.6488696126549058
2213	23	-5.0595426078159065
2213	24	-2.657842832824084
2213	25	-1.8628638069644303
2214	1	-0.3185966346677951
2214	2	-0.125833975991263
2214	3	-0.23728215174682887
2214	4	-0.007486103764897223
2214	5	-0.18239243130106075
2214	6	-0.3669835259508787
2214	7	-0.4722361872043166
2214	8	-0.5970693944158612
2214	9	-0.4948786157480498
2214	10	-0.12626739632286643
2214	11	-0.7068983883148618
2214	12	-0.43818260881081983
2214	13	-0.47927797151051976
2214	14	0.02323513853697534
2214	15	-0.4305938406076635
2214	16	0.023522583949350917
2214	17	0.14652493070989436
2214	18	0.761550608320249
2214	19	-0.14457994509618288
2214	20	-0.3619460717864911
2214	21	-0.47714327713678983
2214	22	0.048612783503294565
2214	23	1.42456475703451
2214	24	0.5614217800515107
2214	25	0.4923389474349591
2215	1	-2.7537772265829474
2215	2	-4.573503987468072
2215	3	-2.800394364151277
2215	4	-4.453394891791039
2215	5	-5.362507101150452
2215	6	-2.8206068246900706
2215	7	-2.184625491670449
2215	8	-2.70894985551832
2215	9	-2.2946590665361164
2215	10	-3.7516286047112537
2215	11	-2.4467170187272558
2215	12	-2.2758230196183398
2215	13	-2.2637607865865625
2215	14	-1.6629916274753
2215	15	-2.198134856689933
2215	16	-3.080483329635687
2215	17	-7.549564768486402
2215	18	-3.6820943342711523
2215	19	-2.9151583860511963
2215	20	-2.7285306326143974
2215	21	-2.6150213282732544
2215	22	-2.3704900444411763
2215	23	-7.0235575323677
2215	24	-4.454978513335657
2215	25	-3.885627463663983
2216	1	-1.1291218653260096
2216	2	-0.6253435295444234
2216	3	-0.8966456178599458
2216	4	-3.210043219689883
2216	5	-1.581137107067537
2216	6	-1.2395154791616676
2216	7	-1.3101096645164816
2216	8	-1.1760386915311294
2216	9	-1.2927076873516654
2216	10	-0.9996677833694605
2216	11	-1.468945955372476
2216	12	-2.2934232009077604
2216	13	-1.5847563676569223
2216	14	-7.158406935520439
2216	15	-3.613111377192002
2216	16	-1.99977596517261
2216	17	-1.1411851685820835
2216	18	-0.292028323100034
2216	19	-0.8824003948386974
2216	20	-1.2059009288463471
2216	21	-1.4935490231698765
2216	22	-0.9954895198768767
2216	23	0.11721454234704254
2216	24	-0.573106196482037
2216	25	-0.4757035464696031
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	82226.0
3	3.0
4	673.0
5	4088.0
6	4908.0
7	4421.0
8	4069.0
9	3876.0
10	3708.0
11	3571.0
12	3578.0
13	3509.0
14	3514.0
15	3544.0
16	3648.0
17	3917.0
18	4513.0
19	5203.0
20	6532.0
21	8121.0
22	10119.0
23	12247.0
24	14556.0
25	17195.0
26	19473.0
27	22399.0
28	24785.0
29	29835.0
30	35538.0
31	40157.0
32	51837.0
33	67969.0
34	87931.0
35	115949.0
36	162724.0
37	225417.0
38	549427.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.12862778890408	18.28306414167508	12.448782993245715	25.139525076175122
2	13.488992077603967	19.64393001437561	43.19274749278179	23.674330415238632
3	19.880325738721936	23.918252787403375	28.57400839458821	27.627413079286477
4	10.381401276363919	16.426193186240173	39.85858578488599	33.333819752509925
5	12.12151982473944	38.15279602733004	36.66359202669039	13.062092121240127
6	32.05718006443184	36.798495171867884	18.418216007767327	12.726108755932952
7	28.48603676311894	31.06954716373176	21.526091965553196	18.91832410759611
8	24.831967898344757	37.8654831207916	20.240762414311988	17.061786566551657
9	26.073960938915526	15.740199160214651	19.957062234831028	38.228777666038795
10	13.529748701184987	30.113901698674916	35.30660790380013	21.049741696339968
11	34.71655211616971	22.985588220095025	23.366355895880133	18.931503767855133
12	22.551329846089494	25.334376588288794	32.19681770761139	19.917475858010324
13	28.936171247546227	21.674285085508874	25.53867459573948	23.850869071205416
14	19.97459918325856	22.32639380750218	28.830656560651597	28.868350448587666
15	23.606570732528823	30.35835771813019	23.256986069507157	22.77808547983383
16	21.421436453339737	28.9434982049653	28.54162930177558	21.093436039919382
17	21.5730360305531	28.590120158590125	28.48074136810432	21.35610244275245
18	20.913520726877	27.95588642090896	30.456122005416926	20.674470846797114
19	22.72290312636239	27.73175923780894	28.250174659664474	21.2951629761642
20	22.64230783494863	27.83541069201853	28.820942645201807	20.701338827831034
21	22.49486539366531	27.248313088777316	28.10827604908307	22.148545468474307
22	21.900013863654603	28.253094392030103	28.019540456333136	21.827351287982157
23	20.777718125212424	27.5930878957919	29.635029661782113	21.994164317213563
24	21.955108228293383	28.062252323303944	28.55485417440696	21.427785273995717
25	22.055497321078963	27.47991721246288	28.164179068178115	22.300406398280042
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	124.0
1	124.0
2	248.0
3	372.0
4	372.0
5	372.0
6	775.5
7	1179.0
8	1179.0
9	1179.0
10	1979.0
11	2779.0
12	2779.0
13	2779.0
14	5156.0
15	7533.0
16	7533.0
17	7533.0
18	13362.5
19	19192.0
20	19192.0
21	19192.0
22	30615.5
23	42039.0
24	42039.0
25	42039.0
26	59620.5
27	77202.0
28	77202.0
29	77202.0
30	99195.5
31	121189.0
32	121189.0
33	121189.0
34	140309.5
35	159430.0
36	159430.0
37	159430.0
38	175657.5
39	191885.0
40	191885.0
41	191885.0
42	203654.0
43	215423.0
44	215423.0
45	215423.0
46	226742.0
47	238061.0
48	238061.0
49	238061.0
50	223478.5
51	208896.0
52	208896.0
53	208896.0
54	179453.0
55	150010.0
56	150010.0
57	150010.0
58	125049.0
59	100088.0
60	100088.0
61	100088.0
62	79496.0
63	58904.0
64	58904.0
65	58904.0
66	44411.0
67	29918.0
68	29918.0
69	29918.0
70	21416.5
71	12915.0
72	12915.0
73	12915.0
74	9000.0
75	5085.0
76	5085.0
77	5085.0
78	3531.5
79	1978.0
80	1978.0
81	1978.0
82	1312.0
83	646.0
84	646.0
85	646.0
86	442.5
87	239.0
88	239.0
89	239.0
90	153.5
91	68.0
92	68.0
93	68.0
94	43.0
95	18.0
96	18.0
97	18.0
98	12.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11713004048189257
2	0.04425047715143632
3	0.033370208730959534
4	0.030878080210068198
5	0.03300550699619495
6	0.020727215259120583
7	0.019754677299748356
8	0.02461736709660949
9	0.028811437046402214
10	0.03671330796630156
11	0.02097034974896364
12	0.01993702816713065
13	0.015986092707180977
14	0.021335051483728225
15	0.02255072393294351
16	0.022186022198178924
17	0.026562443015353942
18	0.020362513524356
19	0.03361334322080259
20	0.023280127402472678
21	0.027838899087029988
22	0.03598390449677239
23	0.028629086179019922
24	0.03932700373211442
25	0.029662407760852914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1645180.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.25756749639704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.0232693740741	71.44791050539688
2	8.189285527256583	13.145042719021255
3	1.630179638186349	3.9250275702897905
4	0.47938167743445503	1.5389602933292725
5	0.20007202354177983	0.8028646966772567
6	0.1130361312125111	0.5443202958191822
7	0.07848050004013005	0.44090578203852104
8	0.0494184433403455	0.31729632415637216
9	0.034314864858741884	0.24786248242771983
>10	0.17753520407655	2.504038030837897
>50	0.012984634150959087	0.7081999461149613
>100	0.00974122519724737	1.7324768097884888
>500	7.669188767754574E-4	0.4472298821950108
>1k	0.001457145865873369	1.8355752197659805
>5k	7.669188767754574E-5	0.36228944214145803
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	5886	0.3577724018040579	No Hit
CCCATGTACTCTGCGTTGATACCAC	4396	0.2672048043375193	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2402	0.14600226115075554	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2075	0.12612601660608566	No Hit
GTCCTACAGTGGACATTTCTAAATT	1720	0.10454783063251437	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	6.078362246076417E-5
13	0.0	0.0	0.0	0.0	1.2156724492152834E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCGAG	25	0.006031166	19.000576	18
TCCAACG	115	0.0	16.522243	18
TACGACA	40	0.005277145	14.250867	4
TAGGACC	530	0.0	13.62347	4
CCAACGA	50	0.0014992688	13.300404	19
CCAACGT	95	7.384733E-8	13.000395	19
GCCGGTT	95	7.405288E-8	12.997628	11
GTCCTAC	990	0.0	12.393109	1
CTACGAG	55	0.0030685763	12.090909	3
AATGACG	80	2.871061E-5	11.873195	8
GTCTAAC	130	2.568413E-9	11.705835	1
GACGAAA	65	8.0361444E-4	11.690175	11
AGGACCT	815	0.0	11.42366	5
GCGTTTA	100	1.9317322E-6	11.398959	13
GGACCTG	785	0.0	11.374413	6
TCCTACA	1150	0.0	11.318079	2
TGTAGGA	1025	0.0	11.308005	2
GTAGGAC	985	0.0	11.284265	3
CCTACAG	1070	0.0	10.920561	3
TGACGAA	70	0.0014933754	10.855493	10
>>END_MODULE