FastQCFastQC Report
Thu 26 May 2016
SRR1513049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513049_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645180
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC65980.40105034099612197No Hit
CCCATGTACTCTGCGTTGATACCAC60170.365735056346418No Hit
GTATCAACGCAGAGTACTTTTTTTT32000.19450759187444536No Hit
GAGTACATGGGAAGCAGTGGTATCA26260.1596177925819667No Hit
TATCAACGCAGAGTACTTTTTTTTT24140.1467316646202847No Hit
GGTATCAACGCAGAGTACTTTTTTT20940.12728090543284018No Hit
GTCCTACAGTGGACATTTCTAAATT18980.1153673154305304No Hit
CACACACACACACACACACACACAC18340.1114771635930415No Hit
CATGTACTCTGCGTTGATACCACTG18210.11068697650105155No Hit
GCTTCCCATGTACTCTGCGTTGATA17570.10679682466356265No Hit
GTATCAACGCAGAGTACATGGGAAG17520.10649290655125883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGG400.00533399814.22786610
TCCAACG1750.013.55570518
GTAGGAC9350.012.5262663
TGTAGGA10250.012.3617872
GACGTGG4150.012.3459987
CCAACGT854.006022E-612.27688819
GTACCGT550.003015867412.1189096
AGGACGG550.003015867412.1189095
TAGGACC4050.011.9906664
GTCCTAC9250.011.9505821
GCCGGTT1302.6739144E-911.67556611
CCAACGA1157.212475E-811.54900819
AGGACGT7200.011.505755
CTGTAGG10450.011.4901861
GGACGTG7100.011.2654646
ATCCCGT1354.843969E-911.24177110
TAGGACG7450.011.119654
CAGGACG700.001462973110.8822854
CGGTTTC1502.1664164E-910.75615513
CCGGGAC803.6911658E-410.712253
Produced by FastQC (version 0.11.5)