FastQCFastQC Report
Thu 26 May 2016
SRR1513048_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513048_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185101
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC75290.3445607319753183No Hit
CCCATGTACTCTGCGTTGATACCAC61740.2825498684042522No Hit
GAGTACATGGGAAGCAGTGGTATCA30150.137979892004992No Hit
GTATCAACGCAGAGTACTTTTTTTT28840.13198474578520628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGA551.9612053E-413.81778218
ACCGCAC550.00307190112.0894538
CGAGCGA803.776196E-410.68621210
GTATTAG1951.4551915E-1110.2424331
GGGCGTT750.002651961510.13164911
GAACAGT3500.010.0418776
GATCGAG951.647277E-49.99833811
TGTAGGA7350.09.9536892
CGTGCAC1252.574745E-69.8788110
GCGTTTA1252.575498E-69.87858313
GTATTAA2900.09.8387911
GTGTAGC5650.09.763331
TAGACAG3900.09.7439775
GTAGGAC6100.09.5008133
GTGTAGG3550.09.3768751
GTCTAAA2252.5465852E-119.2994791
GTTTAAG2151.03682396E-109.2896481
TAAGGTG1554.2587817E-79.1939145
TAGGACA4050.09.149564
GTATAAA2401.0913936E-119.1145461
Produced by FastQC (version 0.11.5)