##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513048_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2185101
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.33775372396974	31.0	30.0	34.0	25.0	34.0
2	29.031346377123988	31.0	30.0	34.0	16.0	34.0
3	29.39424218834736	31.0	30.0	34.0	23.0	34.0
4	32.26491681620209	35.0	33.0	37.0	19.0	37.0
5	32.485676863449335	35.0	33.0	37.0	22.0	37.0
6	32.985759468326634	37.0	35.0	37.0	27.0	37.0
7	32.96738457398537	37.0	35.0	37.0	26.0	37.0
8	32.95563637561833	37.0	35.0	37.0	26.0	37.0
9	34.34644760127793	39.0	35.0	39.0	26.0	39.0
10	33.84491243196539	39.0	34.0	39.0	22.0	39.0
11	34.22668975026784	39.0	35.0	39.0	26.0	39.0
12	34.06821469579667	39.0	35.0	39.0	25.0	39.0
13	34.14617219066761	39.0	35.0	39.0	25.0	39.0
14	34.4623799998261	40.0	34.0	41.0	17.0	41.0
15	35.00400164569052	40.0	34.0	41.0	24.0	41.0
16	34.58742913943108	39.0	34.0	41.0	17.0	41.0
17	34.11911531778165	39.0	33.0	41.0	16.0	41.0
18	33.527228260844694	39.0	32.0	41.0	10.0	41.0
19	34.42398863942674	39.0	34.0	41.0	18.0	41.0
20	34.69529921042551	39.0	34.0	41.0	18.0	41.0
21	34.71260138547372	40.0	34.0	41.0	18.0	41.0
22	34.30201670311807	40.0	34.0	41.0	11.0	41.0
23	32.59788494902524	39.0	31.0	41.0	8.0	41.0
24	33.71036762145091	39.0	33.0	41.0	2.0	41.0
25	33.452126469211265	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7735569500195538
1101	2	1.0983846009717624
1101	3	0.8860181379153254
1101	4	0.9348748940622187
1101	5	1.1436232097596744
1101	6	0.7432686753302846
1101	7	0.6716294613351508
1101	8	0.724863835775956
1101	9	0.8187287159326644
1101	10	1.019151556983772
1101	11	0.7140120248264239
1101	12	0.7883215446398708
1101	13	0.6572296338774777
1101	14	-0.23938601144495664
1101	15	0.39013180380650425
1101	16	1.102032000526954
1101	17	1.7145514229536687
1101	18	-4.64797251994618
1101	19	-0.9059470670453749
1101	20	0.1503176724566515
1101	21	0.5251968271087009
1101	22	1.1498888138323693
1101	23	2.924271270686134
1101	24	1.8353374219608796
1101	25	2.09630754417185
1102	1	1.06099052382773
1102	2	1.3623506347592311
1102	3	1.094642784043323
1102	4	1.6508808392493535
1102	5	1.4396542061284876
1102	6	1.0533506072686194
1102	7	0.9688538948993326
1102	8	0.9538959829592102
1102	9	1.02858117244714
1102	10	1.3852461162575906
1102	11	1.0874899455508498
1102	12	1.112247623030001
1102	13	0.9893662301890984
1102	14	1.9806134110854359
1102	15	1.4726923590572838
1102	16	1.756989233402578
1102	17	1.154794455164101
1102	18	1.5973901928410612
1102	19	1.3753966772400759
1102	20	1.3379686223254197
1102	21	1.395115197654448
1102	22	1.7084017470892618
1102	23	3.329282056993499
1102	24	2.2266217556228156
1102	25	2.484850547096322
1103	1	0.6694083024143467
1103	2	0.9848813176835058
1103	3	0.848778188732549
1103	4	1.3184946714507788
1103	5	1.2057412681505966
1103	6	0.7154744639832913
1103	7	0.5783594662180036
1103	8	0.5820498235982683
1103	9	0.6723628308002532
1103	10	0.9408528674660204
1103	11	0.6517314824130978
1103	12	0.8221254428121085
1103	13	0.6421436173357762
1103	14	1.4737502523779895
1103	15	1.1039852193317543
1103	16	1.2782374514353947
1103	17	1.687654067860251
1103	18	1.6543152550892728
1103	19	1.1796728469964108
1103	20	1.1134046355719462
1103	21	1.0530252017629707
1103	22	1.2852707790502365
1103	23	2.8587688228712764
1103	24	1.7004226330262355
1103	25	1.8907141497445252
1104	1	0.48401146892265956
1104	2	0.9885747643793614
1104	3	0.7198079968373996
1104	4	1.2266921312522783
1104	5	1.007611476268906
1104	6	0.6255388798813897
1104	7	0.49195103224753467
1104	8	0.5087062020508171
1104	9	0.6161011923504205
1104	10	1.052432134201645
1104	11	0.5679582946668589
1104	12	0.6336281022637209
1104	13	0.43866572794220815
1104	14	1.3403730167516485
1104	15	0.8206934786169242
1104	16	1.0341408870854636
1104	17	1.5884123432392343
1104	18	2.1863796347716757
1104	19	1.293903620064789
1104	20	0.7245727802455235
1104	21	0.7741918719403387
1104	22	1.133405245713135
1104	23	2.501922818294055
1104	24	1.6282264577733088
1104	25	1.6047740438542633
1105	1	0.7410256147871905
1105	2	1.0483388762096588
1105	3	0.6862409617759546
1105	4	1.074942771714646
1105	5	0.9684287928862716
1105	6	0.6616708584460156
1105	7	0.3715626707173527
1105	8	0.43085753681152283
1105	9	0.5866120771166976
1105	10	1.0444959700452614
1105	11	0.6254297368519843
1105	12	0.6240985172338327
1105	13	0.6115781918968608
1105	14	1.2577781231240976
1105	15	0.6663575174283238
1105	16	1.0784039531366219
1105	17	1.648470387075328
1105	18	1.4618898388533097
1105	19	1.1001157956566772
1105	20	0.8142529822232234
1105	21	0.7164852841505862
1105	22	1.1598838784033205
1105	23	2.6536010153460765
1105	24	1.6775544363874317
1105	25	1.6539658855393924
1106	1	-0.6193845286516257
1106	2	-0.09117053580256851
1106	3	-0.35599552044580207
1106	4	-2.9249149033141286
1106	5	-0.6864455279760939
1106	6	-0.5848916840967746
1106	7	-0.5235128852782367
1106	8	-0.4952355808479112
1106	9	-0.5179194262062836
1106	10	-0.3174890301403792
1106	11	-0.4472024087897779
1106	12	-0.35242405055616643
1106	13	-0.3369558608995149
1106	14	0.2933748360360653
1106	15	-0.3630542472775531
1106	16	-1.3742884640364323
1106	17	-5.967075831412071
1106	18	-6.963408115379931
1106	19	-3.0535797031614074
1106	20	-1.7026558431626029
1106	21	-1.3237462723895561
1106	22	-1.620384930151026
1106	23	-5.186676332706551
1106	24	-2.3359942820690236
1106	25	-0.9082641611184457
1107	1	0.6662408206825248
1107	2	1.001489628035813
1107	3	0.7760115865659039
1107	4	1.1143912805161804
1107	5	1.0984851403814346
1107	6	0.6153674744389903
1107	7	0.457222299852468
1107	8	0.4810084563260304
1107	9	0.5379910506815762
1107	10	0.9227782589982496
1107	11	0.6003040232117627
1107	12	0.6876099533687778
1107	13	0.6420374825681279
1107	14	1.4889654548656779
1107	15	0.9479094134643589
1107	16	-5.824850393604997
1107	17	-0.8416761683323415
1107	18	0.8654630741088383
1107	19	0.8112229737704695
1107	20	0.706635125027745
1107	21	0.7429191774039694
1107	22	0.9123281641246237
1107	23	2.7717046565687795
1107	24	1.8169769499866746
1107	25	1.5398180054639212
1108	1	0.4413916453004383
1108	2	0.7004092140030238
1108	3	0.4495113516555307
1108	4	0.9714947357394834
1108	5	0.5741502493737727
1108	6	0.2868818343668451
1108	7	0.3175696024647934
1108	8	0.30237592128269597
1108	9	0.3389727241987117
1108	10	0.7861730569275593
1108	11	0.39078107798278694
1108	12	0.5011534699727278
1108	13	0.26241076861253987
1108	14	0.9119767713798339
1108	15	0.6315004245168652
1108	16	0.6979155913728974
1108	17	0.8561564035324238
1108	18	0.8314718469908868
1108	19	0.7923743038147677
1108	20	0.5474778323415777
1108	21	0.6496603669623155
1108	22	0.8995025787809467
1108	23	2.6142315974451833
1108	24	1.396753468154671
1108	25	1.6095007592214046
1109	1	0.21647280245991496
1109	2	0.5477692642006318
1109	3	0.4517841878640425
1109	4	0.401157949868022
1109	5	0.3202701746002461
1109	6	0.16683503555380952
1109	7	0.15789022226583427
1109	8	0.0177981781707075
1109	9	0.20743407160364313
1109	10	0.6358403670415953
1109	11	0.24607993241407655
1109	12	0.3428545240353813
1109	13	0.12115809423902846
1109	14	-0.9875473971133246
1109	15	-0.14040460090874518
1109	16	-0.4004264059865008
1109	17	0.5538142841649503
1109	18	1.3807042854530636
1109	19	0.5846185116991052
1109	20	0.43072438016656633
1109	21	0.34049189559281956
1109	22	0.19734695747659714
1109	23	-4.447983372074582
1109	24	-1.6303175100451028
1109	25	-0.2888294730732639
1110	1	0.47844944881083507
1110	2	0.7383737459795832
1110	3	0.5140208746656967
1110	4	1.1014350213160213
1110	5	0.8434482603669906
1110	6	0.39537636029425016
1110	7	0.28295120764035886
1110	8	0.2736342603295938
1110	9	0.333107410297643
1110	10	0.6945174059527659
1110	11	0.2781972581916392
1110	12	0.4883906657603916
1110	13	0.3415884277161183
1110	14	1.1346469579160328
1110	15	0.572725959055191
1110	16	0.9904692126411732
1110	17	1.2626945511703198
1110	18	1.854437149600784
1110	19	0.9930617345060639
1110	20	0.6645319265082463
1110	21	0.5156439247810667
1110	22	0.7495223920000385
1110	23	2.284039986658378
1110	24	1.1137526949454042
1110	25	1.5213221028023725
1111	1	0.6034026416128953
1111	2	0.9287981143247137
1111	3	0.7244324260956283
1111	4	1.1979743626982895
1111	5	0.9375379453292823
1111	6	0.5814217130060939
1111	7	0.412756458915041
1111	8	0.21663326374496705
1111	9	0.32206735914312645
1111	10	0.6378509336834668
1111	11	0.37804448848255134
1111	12	0.49415128927844165
1111	13	0.41178828457199756
1111	14	-0.3887735294977972
1111	15	0.18794421142063555
1111	16	0.65644397997373
1111	17	1.1921863762063651
1111	18	1.933377483724101
1111	19	0.9408127136406819
1111	20	0.7559113904756671
1111	21	0.6057689849617489
1111	22	0.9810339335880798
1111	23	0.9022340994464031
1111	24	0.7494315892065231
1111	25	0.38654233103265057
1112	1	0.48710864583189917
1112	2	0.7925064805018032
1112	3	0.5616099913044792
1112	4	1.1534328100574243
1112	5	0.9803993540713805
1112	6	0.2846399548124552
1112	7	0.31804850977631105
1112	8	0.3158143147309076
1112	9	0.5232720234683157
1112	10	0.8609435416580453
1112	11	0.472902154227377
1112	12	0.517419450855428
1112	13	0.43222316408995454
1112	14	0.7825655052760467
1112	15	0.5323519471454503
1112	16	0.9238659257180117
1112	17	1.1114450235927933
1112	18	1.1544511759951277
1112	19	0.614146108295138
1112	20	0.5982464997671499
1112	21	0.5988630498604053
1112	22	0.6557969041954976
1112	23	2.2711208698366434
1112	24	1.2935653016576723
1112	25	1.3292690358925157
1113	1	0.42020494392100005
1113	2	0.8993765119105639
1113	3	0.6209729373795803
1113	4	0.7711569352019367
1113	5	0.9013343430813947
1113	6	0.39981501003745024
1113	7	0.35831721371436487
1113	8	0.322682048805774
1113	9	0.5593733313631475
1113	10	0.7575214525014289
1113	11	0.5641448144882588
1113	12	0.5763428295929245
1113	13	0.4392738759560757
1113	14	1.2357634749505877
1113	15	0.7143038655648581
1113	16	1.0859615989970095
1113	17	1.4903163143288154
1113	18	2.088067933460408
1113	19	1.2427555515022917
1113	20	0.9115175829018369
1113	21	0.9412745191866279
1113	22	1.1201400171605869
1113	23	0.8970157380133585
1113	24	0.869361788937077
1113	25	1.2307695508512353
1114	1	-0.06237405731793544
1114	2	-2.8446473478158154
1114	3	-0.876992348425766
1114	4	0.2491243430773693
1114	5	0.6389172414302422
1114	6	0.389720897026848
1114	7	0.3026530815652393
1114	8	0.31745377868379876
1114	9	0.45734188802840237
1114	10	0.5990778872003091
1114	11	0.3390112876157474
1114	12	0.33683475096783155
1114	13	0.3135899670710671
1114	14	1.0636460399110632
1114	15	0.531220593460425
1114	16	0.8376200446365161
1114	17	1.18359844673801
1114	18	1.4263316493327736
1114	19	0.879200509711076
1114	20	0.7029736181569177
1114	21	0.544204810779803
1114	22	0.7310910954673631
1114	23	2.257525369898019
1114	24	1.226106262181041
1114	25	1.1953850692466261
1115	1	0.33177512213730154
1115	2	0.6046142165344683
1115	3	0.3728023787955905
1115	4	0.46763003529932945
1115	5	0.5081635547155301
1115	6	0.058814621611659845
1115	7	-0.07136921901967241
1115	8	-0.04915333546191647
1115	9	0.016955912764167635
1115	10	0.08573133211461936
1115	11	0.159649084968585
1115	12	0.04018381927917858
1115	13	0.011723155542696873
1115	14	0.8015673369228224
1115	15	0.07839969278903425
1115	16	0.6011111492226036
1115	17	1.057341482683377
1115	18	1.4216737524187337
1115	19	0.6808877270707541
1115	20	0.17028029881023343
1115	21	0.19608278352769304
1115	22	0.5332502060664623
1115	23	2.275931079447183
1115	24	0.9687501599598818
1115	25	0.7391150395406143
1116	1	0.4198522409790719
1116	2	0.40954227476653315
1116	3	0.39921882808171105
1116	4	1.1296398097655356
1116	5	0.47319120740621656
1116	6	0.37561790223168146
1116	7	0.3810091244580889
1116	8	0.24926037565406745
1116	9	0.3526353654129082
1116	10	0.8609094781236521
1116	11	0.35213602446256687
1116	12	0.3790718441442138
1116	13	0.355253050375282
1116	14	1.01029537206189
1116	15	0.44773631098396294
1116	16	0.8331271800069899
1116	17	1.12343871835688
1116	18	1.5487500606490343
1116	19	0.7322584616402636
1116	20	0.4838832547143852
1116	21	0.49371209844318287
1116	22	0.8749017326787936
1116	23	2.241651522173008
1116	24	1.1566983232415353
1116	25	1.4333958103755648
1201	1	0.974599389869578
1201	2	1.2632209413467272
1201	3	1.0487568104480367
1201	4	1.5057894084254855
1201	5	1.4002166268103409
1201	6	0.8897244989086772
1201	7	0.7480597058164946
1201	8	0.8195609663474883
1201	9	0.9362973075803751
1201	10	1.3621247494187827
1201	11	1.0888196284457692
1201	12	1.112337441313187
1201	13	0.9992840301716939
1201	14	1.8917347295247566
1201	15	1.3267201506025472
1201	16	1.7348721559685103
1201	17	2.0526893100656878
1201	18	2.6584289296516133
1201	19	1.7092343890983699
1201	20	1.4491818997945671
1201	21	1.3962118183927927
1201	22	1.5642732799522818
1201	23	3.2753648396663024
1201	24	2.1676245465049107
1201	25	2.3632390116364803
1202	1	0.9038688569598676
1202	2	1.2394274551554965
1202	3	1.1301952684296168
1202	4	1.6980764778189865
1202	5	1.5580885758426888
1202	6	1.0939580327453555
1202	7	0.9521036267501799
1202	8	0.9174268655549795
1202	9	0.9709592842608288
1202	10	1.419564212613679
1202	11	1.0742238819249152
1202	12	1.1809867739164517
1202	13	1.1030925529310593
1202	14	1.9723212540839299
1202	15	1.4017508467931918
1202	16	1.6319372339742557
1202	17	2.0931907775314116
1202	18	2.9282397642880653
1202	19	2.029251295302714
1202	20	1.720134101920408
1202	21	1.6404301150728315
1202	22	1.9890216052511889
1202	23	3.537805939265965
1202	24	2.2926712282743793
1202	25	2.5043035366992115
1203	1	1.0587531883256531
1203	2	1.4011236571908086
1203	3	1.0896616786754976
1203	4	1.7911273154959702
1203	5	1.5877397259446582
1203	6	1.1091216119681704
1203	7	1.055117033355053
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2211	18	-5.623052413909864
2211	19	-1.799940088766057
2211	20	-1.0091305091043452
2211	21	-0.7273857125239473
2211	22	-0.420167452473855
2211	23	-5.11723456026348
2211	24	-2.23949100247777
2211	25	-1.408891326310865
2212	1	0.25206171239455344
2212	2	0.5000878926479189
2212	3	0.3047289510310591
2212	4	0.8162333473508454
2212	5	0.7940917313289759
2212	6	0.3059602573739042
2212	7	0.17874891155032202
2212	8	0.16171522642201808
2212	9	-0.07406506502729115
2212	10	-3.641153537282424
2212	11	-1.0234862651923962
2212	12	-0.20504700236014628
2212	13	-0.04369381789715732
2212	14	0.8954619095099403
2212	15	0.4249480823671732
2212	16	0.6535171667388937
2212	17	1.2070651120940425
2212	18	1.6959894192795417
2212	19	0.8664708525309095
2212	20	0.47945272548686546
2212	21	0.35307556382681327
2212	22	0.4461330315299179
2212	23	1.8995835667824892
2212	24	1.0538069175779299
2212	25	0.8405424963817509
2213	1	-1.5831222261673723
2213	2	-3.89747581330251
2213	3	-1.7424717354241395
2213	4	-3.405223840384739
2213	5	-1.1855297602319474
2213	6	-0.9476601466833117
2213	7	-0.8899382933046169
2213	8	-0.8002439947101792
2213	9	-0.69453492713955
2213	10	-0.7500042542868997
2213	11	-0.8418826426452668
2213	12	-0.6906370739723471
2213	13	-1.0766122357893693
2213	14	-6.217910940380094
2213	15	-2.8224974184854332
2213	16	-1.9377722143000469
2213	17	-0.7902996664006139
2213	18	-0.1365046704655697
2213	19	-0.7373337296079754
2213	20	-0.7861308076563631
2213	21	-1.0174003913317264
2213	22	-0.7989105484583305
2213	23	-5.613123250117688
2213	24	-3.064726112195636
2213	25	-1.9872002778956812
2214	1	-0.28581694556650916
2214	2	-0.1594553400045733
2214	3	-0.22451462018502966
2214	4	0.07427761323902615
2214	5	-0.07869413329439112
2214	6	-0.36248931524973926
2214	7	-0.5057952939860826
2214	8	-0.5178549039971898
2214	9	-0.4252044275146005
2214	10	-0.0790525433578324
2214	11	-0.5181969097296388
2214	12	-0.4332903499778311
2214	13	-0.5071259148874177
2214	14	0.0144932667126767
2214	15	-0.33142744026936555
2214	16	0.046576612914009274
2214	17	0.2819915191974829
2214	18	0.851142142266184
2214	19	-0.08092185156529297
2214	20	-0.20762213248857364
2214	21	-0.3815034016974792
2214	22	0.09247752741445936
2214	23	1.63733743176995
2214	24	0.5115822717571419
2214	25	0.418396459581011
2215	1	-2.7018590909623263
2215	2	-4.700608503572131
2215	3	-2.680240651616291
2215	4	-4.151001713963442
2215	5	-5.004474393284461
2215	6	-2.505710118841165
2215	7	-1.895442056484729
2215	8	-2.201619935505203
2215	9	-1.940767256359429
2215	10	-3.404874234468082
2215	11	-2.2963418763576158
2215	12	-1.8863667477103192
2215	13	-1.913602170704614
2215	14	-1.166211970838802
2215	15	-1.6771458590259911
2215	16	-2.688846681557088
2215	17	-6.793892201113479
2215	18	-3.1843225050053974
2215	19	-2.472244185680257
2215	20	-2.2628637565884873
2215	21	-2.253220357442153
2215	22	-1.9000835110420837
2215	23	-6.422272238519788
2215	24	-3.7673037558521187
2215	25	-3.54704771492154
2216	1	-0.9849541340968386
2216	2	-0.5164178468786886
2216	3	-0.716494189934398
2216	4	-3.3649746586721427
2216	5	-1.3413563184975281
2216	6	-0.9714723101296201
2216	7	-1.0255390746371056
2216	8	-0.9678609306197927
2216	9	-0.9715463303704652
2216	10	-0.5256412866312488
2216	11	-0.9924026510921067
2216	12	-1.7787015163050839
2216	13	-1.1520090611861917
2216	14	-7.130605833562793
2216	15	-3.3025514430516516
2216	16	-1.6532677621126695
2216	17	-0.713668370866877
2216	18	-0.043094083762298396
2216	19	-0.6111221363232104
2216	20	-1.0696225191968551
2216	21	-1.0981855952876671
2216	22	-0.7235278011243622
2216	23	0.5008096327176332
2216	24	-0.2884142127130076
2216	25	-0.2583631258877972
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	100002.0
3	4.0
4	862.0
5	5211.0
6	6214.0
7	5527.0
8	5072.0
9	4909.0
10	4808.0
11	4675.0
12	4651.0
13	4611.0
14	4481.0
15	4636.0
16	4897.0
17	5020.0
18	5782.0
19	6638.0
20	8525.0
21	10539.0
22	12762.0
23	15502.0
24	18520.0
25	21774.0
26	24924.0
27	28420.0
28	32064.0
29	38473.0
30	45504.0
31	51966.0
32	67176.0
33	88535.0
34	116643.0
35	153521.0
36	217166.0
37	304295.0
38	750792.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.00295959375681	17.61384356851872	11.91772512612634	25.465471711598127
2	14.519313359742576	19.758809642852565	40.61991205466457	25.101964942740295
3	19.21673222434644	24.40677624687777	28.750979702756354	27.625511826019437
4	10.948183672647234	16.111642147437657	38.50553559591432	34.434638584000794
5	12.815372550599466	37.54507611150355	35.87140587109914	13.76814546679784
6	33.02128224435102	35.78474700013961	17.85781474217107	13.336156013338307
7	28.869431241440424	30.987399573760648	21.6761844254191	18.46698475937983
8	26.508337655239593	35.234254948372865	20.07322140778639	18.184185988601154
9	27.152745638477622	14.76456179237272	19.13466736797099	38.948025201178666
10	14.503603617214042	28.59591463389511	34.5689050158561	22.33157673303475
11	35.087862240063714	22.463987696082246	23.070303524102037	19.377846539752
12	23.317376792250048	24.674232062852113	30.98988228961259	21.01850885528525
13	29.211417774602253	20.980982428805977	25.256766275977004	24.550833520614766
14	21.543601611978886	20.812910478778235	27.77802699204081	29.865460917202068
15	24.722266724646968	28.292632687158125	22.940150596205342	24.044949991989565
16	23.09089119742567	27.248546102456007	26.695641625985	22.964921074133326
17	22.69919721899117	27.138044733629325	27.44337342889618	22.719384618483325
18	22.398178239575227	26.62017668329748	28.659083627042612	22.32256145008468
19	23.759308107632993	26.726549245417246	27.269310460696968	22.244832186252797
20	24.05473176546197	26.388412070210904	27.165023816520783	22.391832347806346
21	23.91540001062032	26.050679059479275	27.01543425199085	23.018486677909557
22	23.624886749185443	26.36373666570374	26.568698321804906	23.442678263305915
23	22.03383313053963	26.041503584824987	28.609521873795487	23.315141410839896
24	23.31022554132849	26.869469512731108	26.654980002344047	23.165324943596353
25	23.39126593375404	26.002009672981803	26.87184558020724	23.734878813056923
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	280.0
1	280.0
2	401.5
3	523.0
4	523.0
5	523.0
6	1090.0
7	1657.0
8	1657.0
9	1657.0
10	2660.0
11	3663.0
12	3663.0
13	3663.0
14	6098.5
15	8534.0
16	8534.0
17	8534.0
18	15474.5
19	22415.0
20	22415.0
21	22415.0
22	34238.0
23	46061.0
24	46061.0
25	46061.0
26	64311.0
27	82561.0
28	82561.0
29	82561.0
30	105636.0
31	128711.0
32	128711.0
33	128711.0
34	152260.0
35	175809.0
36	175809.0
37	175809.0
38	198742.5
39	221676.0
40	221676.0
41	221676.0
42	241552.0
43	261428.0
44	261428.0
45	261428.0
46	284268.0
47	307108.0
48	307108.0
49	307108.0
50	299535.0
51	291962.0
52	291962.0
53	291962.0
54	263421.0
55	234880.0
56	234880.0
57	234880.0
58	203798.5
59	172717.0
60	172717.0
61	172717.0
62	142437.0
63	112157.0
64	112157.0
65	112157.0
66	87695.0
67	63233.0
68	63233.0
69	63233.0
70	46467.0
71	29701.0
72	29701.0
73	29701.0
74	21027.5
75	12354.0
76	12354.0
77	12354.0
78	8658.5
79	4963.0
80	4963.0
81	4963.0
82	3435.5
83	1908.0
84	1908.0
85	1908.0
86	1246.0
87	584.0
88	584.0
89	584.0
90	373.5
91	163.0
92	163.0
93	163.0
94	99.0
95	35.0
96	35.0
97	35.0
98	26.5
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10841604118070514
2	0.04654247103451969
3	0.0350098233445502
4	0.03171478114741607
5	0.0338657114705453
6	0.01903802158344168
7	0.01890072815856109
8	0.022607650630336998
9	0.02745868497611781
10	0.035147116769430796
11	0.01972448870784463
12	0.01908378605840188
13	0.015193805686785187
14	0.02091436505680973
15	0.020639778207048553
16	0.020868600581849535
17	0.02608575072731192
18	0.018351554459038737
19	0.03272159959654039
20	0.022104241405774838
21	0.027870565250759576
22	0.03615393521855512
23	0.028145152100520758
24	0.038670981341365915
25	0.030479140323490764
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2185101.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.8007344037815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.26523693229171	61.345711333794895
2	10.437337176292385	15.196916233079536
3	2.723031426393284	5.9471606293802335
4	1.0436545331667382	3.039152659134971
5	0.4687980415187723	1.7064420854810536
6	0.2743403836904667	1.1983308845568708
7	0.16715131141538173	0.8518116759316482
8	0.1260130124886756	0.7339071882886781
9	0.08495992229086277	0.5566630263896715
>10	0.3768173834413204	4.7436822684173245
>50	0.020990806620538587	1.0635095882697458
>100	0.009185169179193379	1.2233600466118857
>500	0.001592244365768276	0.7474299735810521
>1k	7.642772955683954E-4	1.0105605629151695
>5k	1.2737954926139925E-4	0.635361844167312
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	7529	0.3445607319753183	No Hit
CCCATGTACTCTGCGTTGATACCAC	6174	0.2825498684042522	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3015	0.137979892004992	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2884	0.13198474578520628	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.576447496019635E-5	0.0
5	0.0	0.0	0.0	4.576447496019635E-5	4.576447496019635E-5
6	2.2882237480098173E-4	0.0	0.0	4.576447496019635E-5	9.15289499203927E-5
7	2.2882237480098173E-4	0.0	0.0	4.576447496019635E-5	9.15289499203927E-5
8	2.2882237480098173E-4	0.0	0.0	4.576447496019635E-5	9.15289499203927E-5
9	2.2882237480098173E-4	0.0	0.0	4.576447496019635E-5	9.15289499203927E-5
10	2.2882237480098173E-4	0.0	0.0	4.576447496019635E-5	9.15289499203927E-5
11	2.2882237480098173E-4	0.0	0.0	4.576447496019635E-5	9.15289499203927E-5
12	2.2882237480098173E-4	0.0	0.0	4.576447496019635E-5	1.830578998407854E-4
13	2.2882237480098173E-4	0.0	0.0	4.576447496019635E-5	1.830578998407854E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGA	55	1.9612053E-4	13.817782	18
ACCGCAC	55	0.003071901	12.089453	8
CGAGCGA	80	3.776196E-4	10.686212	10
GTATTAG	195	1.4551915E-11	10.242433	1
GGGCGTT	75	0.0026519615	10.131649	11
GAACAGT	350	0.0	10.041877	6
GATCGAG	95	1.647277E-4	9.998338	11
TGTAGGA	735	0.0	9.953689	2
CGTGCAC	125	2.574745E-6	9.87881	10
GCGTTTA	125	2.575498E-6	9.878583	13
GTATTAA	290	0.0	9.838791	1
GTGTAGC	565	0.0	9.76333	1
TAGACAG	390	0.0	9.743977	5
GTAGGAC	610	0.0	9.500813	3
GTGTAGG	355	0.0	9.376875	1
GTCTAAA	225	2.5465852E-11	9.299479	1
GTTTAAG	215	1.03682396E-10	9.289648	1
TAAGGTG	155	4.2587817E-7	9.193914	5
TAGGACA	405	0.0	9.14956	4
GTATAAA	240	1.0913936E-11	9.114546	1
>>END_MODULE