FastQCFastQC Report
Thu 26 May 2016
SRR1513048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513048_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185101
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC89210.4082648811199116No Hit
CCCATGTACTCTGCGTTGATACCAC80920.3703261313779088No Hit
GTATCAACGCAGAGTACTTTTTTTT44030.2015009832497445No Hit
GAGTACATGGGAAGCAGTGGTATCA31800.1455310303734244No Hit
TATCAACGCAGAGTACTTTTTTTTT30260.13848330122955416No Hit
GGTATCAACGCAGAGTACTTTTTTT25200.11532647689969479No Hit
CATGTACTCTGCGTTGATACCACTG23300.10663122665725748No Hit
GTATCAACGCAGAGTACATGGGAAG22850.10457182528404865No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATC500.001509182213.28909915
CCGCTCA905.49735E-712.6496229
TAGGACC3050.011.2371214
ACCGCTC855.3984477E-511.1614318
ATTGGAC1303.731784E-710.2526723
TAATACT2800.010.2003624
CTAATAC2250.010.1550273
CTAGCAC1701.5024852E-910.08035853
GTAGGAC4550.010.0434343
GTCCTAC4750.09.8297861
GTATTAC1803.970854E-99.5288731
TGTACTG4200.09.5203395
TCTAGAC1901.0113581E-99.5203383
TAGGACG3700.09.5203384
GCACCGT900.0010925449.520126
AAAGGCG1951.6189006E-99.2762285
CTACACT3700.09.2630324
AGAACCG1356.665314E-69.1677335
GTCCTAG3650.09.1372771
TAGACTG3450.09.1064115
Produced by FastQC (version 0.11.5)