FastQCFastQC Report
Thu 26 May 2016
SRR1513047_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513047_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109633
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC55880.2648801948016551No Hit
CCCATGTACTCTGCGTTGATACCAC45280.21463448855796244No Hit
GAGTACATGGGAAGCAGTGGTATCA21660.10267188653192284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7150.012.2253934
TGTAGGA12450.012.1329842
GTAGGAC11200.012.1296313
GACCGTC550.003070935112.0899297
TCCAACG1604.5474735E-1111.281939518
GTCCTAC10500.011.1384951
CGGGGAC600.00587623711.0834863
AGGACCT9700.010.97026355
CTAGGAC3600.010.8195923
TGGCGAG8750.010.64065118
GTCTAGG2250.010.564971
TGTAGAA7150.010.3640242
GTCCTAG3700.010.2794291
ACCGTGG1303.8469807E-710.2299398
GGACCTG9300.010.2149526
TTGGACA2700.010.2050544
CTGTAGG13700.010.2025221
ATTGGAC2053.6379788E-1210.1952623
TAGGCGT750.002645336610.1346744
GGCGAGG3950.010.10188419
Produced by FastQC (version 0.11.5)