##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513047_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2109633
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.19639861530418	31.0	30.0	34.0	24.0	34.0
2	28.917552958263357	31.0	30.0	34.0	16.0	34.0
3	29.26393358465667	31.0	30.0	34.0	22.0	34.0
4	32.13289657490189	35.0	32.0	37.0	19.0	37.0
5	32.35164457514648	35.0	33.0	37.0	20.0	37.0
6	32.85245585369588	37.0	33.0	37.0	26.0	37.0
7	32.83699107854304	37.0	35.0	37.0	26.0	37.0
8	32.81513609239143	37.0	35.0	37.0	26.0	37.0
9	34.197654283944175	39.0	35.0	39.0	25.0	39.0
10	33.707422096639554	38.0	34.0	39.0	19.0	39.0
11	34.088347120091505	39.0	34.0	39.0	25.0	39.0
12	33.92861791600719	39.0	34.0	39.0	23.0	39.0
13	34.01869993501239	39.0	35.0	39.0	25.0	39.0
14	34.315993824518294	39.0	33.0	41.0	17.0	41.0
15	34.87124632578273	40.0	34.0	41.0	22.0	41.0
16	34.45452028859996	39.0	34.0	41.0	17.0	41.0
17	33.9953465839793	39.0	33.0	41.0	11.0	41.0
18	33.42164916836246	39.0	32.0	41.0	10.0	41.0
19	34.3122272926144	39.0	34.0	41.0	18.0	41.0
20	34.575902538498404	39.0	34.0	41.0	18.0	41.0
21	34.59121942062909	40.0	34.0	41.0	18.0	41.0
22	34.17458297248858	39.0	34.0	41.0	10.0	41.0
23	32.51066228106974	39.0	31.0	41.0	2.0	41.0
24	33.599687244179435	39.0	33.0	41.0	2.0	41.0
25	33.33539862146639	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8327395126957668
1101	2	1.1364930603308139
1101	3	0.9226576391846812
1101	4	0.8894005108635028
1101	5	1.2326354217149316
1101	6	0.9155758721304537
1101	7	0.7756993535660328
1101	8	0.7874125418562059
1101	9	0.9580090678457793
1101	10	1.3027694498497553
1101	11	0.9149062016177965
1101	12	0.9831395050669869
1101	13	0.9236772703495433
1101	14	0.036707273823203934
1101	15	0.6666272386004977
1101	16	1.1675617545803263
1101	17	1.7761074774804797
1101	18	-4.228085348893657
1101	19	-0.691485495473259
1101	20	0.27673114247698294
1101	21	0.6589087052296279
1101	22	1.1627045519098687
1101	23	3.012436234306165
1101	24	1.9110206663632354
1101	25	2.0086635086499314
1102	1	0.898653088413262
1102	2	1.2161406314816539
1102	3	0.8685603040415941
1102	4	1.4690997676869415
1102	5	1.1611822692160274
1102	6	0.8560831969515021
1102	7	0.8178108466579204
1102	8	0.8301994212443873
1102	9	0.8922682138696345
1102	10	1.1883377733265803
1102	11	0.9388686255802199
1102	12	1.053347772660878
1102	13	0.9374117690513657
1102	14	1.723629087542399
1102	15	1.2097879961403422
1102	16	1.54288680310799
1102	17	1.2461056808120219
1102	18	1.5164229603205328
1102	19	1.3856876052292506
1102	20	1.2547855166097932
1102	21	1.2859771009254715
1102	22	1.6323173522416283
1102	23	3.061553325253321
1102	24	2.112153624358939
1102	25	2.37282374731997
1103	1	0.732073439483159
1103	2	1.0265071036180125
1103	3	0.8263164548506587
1103	4	1.2849397257096697
1103	5	1.1566664802642492
1103	6	0.8261557197612959
1103	7	0.7180689490931513
1103	8	0.7982938211361557
1103	9	1.006290655412684
1103	10	1.2706468165670657
1103	11	0.9871336605288334
1103	12	0.937009675733762
1103	13	0.771885566676012
1103	14	1.603648891855869
1103	15	1.1208940086343588
1103	16	1.4354724904868803
1103	17	1.9085587619926443
1103	18	1.8908139114394515
1103	19	1.3399283197743443
1103	20	1.2342695044429632
1103	21	1.1580748787514281
1103	22	1.4963978557922886
1103	23	3.0282766186869665
1103	24	1.9554407027971479
1103	25	2.177144343545031
1104	1	0.7557388496028778
1104	2	1.1180001586203545
1104	3	0.9094477103673029
1104	4	1.5081085183093137
1104	5	1.3544027440180173
1104	6	0.8808810346681071
1104	7	0.8410299812673401
1104	8	0.8142293564538363
1104	9	0.8950527920310947
1104	10	1.2747028449652404
1104	11	0.8257594543680327
1104	12	0.9926228318759129
1104	13	0.7503352022986363
1104	14	1.660528486404388
1104	15	1.2047183299874789
1104	16	1.3949357070133956
1104	17	1.9094583534821155
1104	18	2.5358496879759045
1104	19	1.697127282246818
1104	20	0.9396276999863105
1104	21	1.1694626674093627
1104	22	1.5253608381103518
1104	23	3.1045098775621938
1104	24	2.1421042440289924
1104	25	2.0850926535796646
1105	1	0.38236695135193344
1105	2	0.7854971396495323
1105	3	0.5125695209707075
1105	4	0.5547736267974912
1105	5	0.7157884516258264
1105	6	0.394490847297142
1105	7	0.160736264622102
1105	8	0.16028297727110186
1105	9	0.3740438714592429
1105	10	0.918025855366082
1105	11	0.4436721768954044
1105	12	0.31569838405541617
1105	13	0.31009991734674713
1105	14	1.0406248986984181
1105	15	0.4692315939818741
1105	16	0.8092925688419186
1105	17	1.2876012968812773
1105	18	1.2644094441209717
1105	19	0.766923939953962
1105	20	0.6502394798156885
1105	21	0.43274535994179786
1105	22	0.8658395884512018
1105	23	2.3293990521171395
1105	24	1.2972071811954464
1105	25	1.2213451369266295
1106	1	-0.5590678884901052
1106	2	-0.08126898584410469
1106	3	-0.3651517017878092
1106	4	-2.5526828599657065
1106	5	-0.45189328012786945
1106	6	-0.5172365185065715
1106	7	-0.46814482357447673
1106	8	-0.46921972859404804
1106	9	-0.47962879198964004
1106	10	-0.29384192289924016
1106	11	-0.43627516843149294
1106	12	-0.4316498681019638
1106	13	-0.37790505559460286
1106	14	0.2464447027060217
1106	15	-0.2570796536413553
1106	16	-1.2712339778891035
1106	17	-6.070807361887187
1106	18	-7.050646867126652
1106	19	-2.994952614090213
1106	20	-1.6247831909463386
1106	21	-1.2751250510571808
1106	22	-1.6271485642473493
1106	23	-5.357563065770822
1106	24	-2.4455996054068656
1106	25	-1.0095894835209336
1107	1	0.45208792461097147
1107	2	0.7048701003735225
1107	3	0.4942720788951114
1107	4	0.8064186009457472
1107	5	0.8752368521163234
1107	6	0.3782457684511087
1107	7	0.33728166831807016
1107	8	0.3899861662505799
1107	9	0.21772923313268677
1107	10	0.6890082216792166
1107	11	0.3894301242453295
1107	12	0.43257380087447217
1107	13	0.3096504340230979
1107	14	1.077071771748777
1107	15	0.4837452871600192
1107	16	-5.558444166451988
1107	17	-1.0955945668395088
1107	18	0.7007805905616067
1107	19	0.6293682744418376
1107	20	0.5159282979499196
1107	21	0.4884996500406871
1107	22	0.6550103964168343
1107	23	2.3652349491117377
1107	24	1.346313019172051
1107	25	1.1812430207407871
1108	1	0.32660058401752323
1108	2	0.5926630771735155
1108	3	0.4433937400205217
1108	4	0.9133681083386733
1108	5	0.7545439201959354
1108	6	0.3478259075473531
1108	7	0.24422012754327227
1108	8	0.3452989992221305
1108	9	0.39376852415707475
1108	10	0.929303198216644
1108	11	0.4523811146496399
1108	12	0.5607683241889632
1108	13	0.4714516135628841
1108	14	1.0003914771004432
1108	15	0.6493999683774518
1108	16	0.9435233233127178
1108	17	0.9184124681146812
1108	18	0.7702892123303471
1108	19	0.700768632562756
1108	20	0.559961734017854
1108	21	0.6624909275218087
1108	22	1.0503480887198293
1108	23	2.5666706193901447
1108	24	1.3938922683088038
1108	25	1.4922926386535522
1109	1	0.4812687401685345
1109	2	0.6635542427970336
1109	3	0.5221627539002718
1109	4	0.45147372145021336
1109	5	0.34420870970425455
1109	6	0.2811673386765534
1109	7	0.21444341629409536
1109	8	0.0715533605547094
1109	9	0.3097250715119628
1109	10	0.8520282276747722
1109	11	0.45495382265460194
1109	12	0.557811895431719
1109	13	0.4854978781197872
1109	14	-0.6472684192987757
1109	15	0.05907667503575453
1109	16	-0.052251348822196064
1109	17	1.0040869252435485
1109	18	1.709498166069615
1109	19	0.8769199861061452
1109	20	0.7276615710417431
1109	21	0.6557251234298107
1109	22	0.5332414311839102
1109	23	-3.7697567414680435
1109	24	-0.9678022241698088
1109	25	0.2018275063597912
1110	1	0.6841421102840926
1110	2	0.9170299778815796
1110	3	0.7807479898757919
1110	4	1.239952897627198
1110	5	0.9953491363997173
1110	6	0.6515771878477921
1110	7	0.5892726940817852
1110	8	0.6396102903587391
1110	9	0.7084413238958192
1110	10	1.005124631559383
1110	11	0.6624000222750368
1110	12	0.7683548465218593
1110	13	0.6613852049355131
1110	14	1.433823183277056
1110	15	0.9416865293049099
1110	16	1.3403149646207737
1110	17	1.6132807827197553
1110	18	2.2471627186465
1110	19	1.3714844503964159
1110	20	0.9615033827479493
1110	21	0.7957843055852507
1110	22	1.1241588009379768
1110	23	2.532259947250971
1110	24	1.6002477539323294
1110	25	1.8046929978798758
1111	1	0.257348874402787
1111	2	0.5702832427173554
1111	3	0.31200087413610333
1111	4	0.8565487902145179
1111	5	0.5599981956506142
1111	6	0.043289682519962014
1111	7	-0.08688741699923952
1111	8	-0.20702810283537332
1111	9	-0.07270202062125009
1111	10	0.13755513020815613
1111	11	0.06572956040015754
1111	12	0.1491570290905102
1111	13	-0.058005566968255096
1111	14	-0.4944613027584879
1111	15	-0.17274329226305696
1111	16	0.195041716409186
1111	17	0.6595775619755884
1111	18	1.2874213653255708
1111	19	0.37931535556487006
1111	20	0.18374453832331028
1111	21	0.08632501653818991
1111	22	0.4495639307517294
1111	23	0.44074221501066546
1111	24	0.2313012654641753
1111	25	-0.21835450761066966
1112	1	0.4994332188087398
1112	2	0.8770768011030157
1112	3	0.6444882348526804
1112	4	1.1769160683705095
1112	5	0.9037199880548741
1112	6	0.3556351262473285
1112	7	0.3996097385527264
1112	8	0.3286043871993769
1112	9	0.5364166865556328
1112	10	0.7904868599675723
1112	11	0.46225521185616003
1112	12	0.6152128473642122
1112	13	0.4909427192664495
1112	14	0.8417849988688175
1112	15	0.5231541757842493
1112	16	1.0940209060730695
1112	17	1.089361223662145
1112	18	1.060845346332954
1112	19	0.72184036113876
1112	20	0.6778048285839091
1112	21	0.6829254872934172
1112	22	0.5763279996373569
1112	23	2.266798255077177
1112	24	1.2432590278859834
1112	25	1.2324287295394072
1113	1	0.4358675633470348
1113	2	0.8267360897379312
1113	3	0.5083780345026021
1113	4	0.6202951826250498
1113	5	0.8749466183604895
1113	6	0.32994984362517243
1113	7	0.299619713554506
1113	8	0.21794787595190712
1113	9	0.34995302418602137
1113	10	0.7520038235423385
1113	11	0.4020565098207385
1113	12	0.4034544020072772
1113	13	0.309527361927195
1113	14	0.9603529008743976
1113	15	0.5066299347951428
1113	16	0.8705954384189596
1113	17	1.321871662718351
1113	18	1.8511957898302072
1113	19	1.0091244023502597
1113	20	0.7480403774085076
1113	21	0.6585117138109666
1113	22	0.8981134902599877
1113	23	0.8466518740945617
1113	24	0.8511674695131362
1113	25	1.2559296602504446
1114	1	-0.1908218686259069
1114	2	-2.7284267265934545
1114	3	-0.8658013507503703
1114	4	0.3035962692957028
1114	5	0.4579229769500799
1114	6	0.25693678247041873
1114	7	0.27249624987764776
1114	8	0.271691671610256
1114	9	0.4277542540821102
1114	10	0.5808841021535045
1114	11	0.39944327378019295
1114	12	0.33847402640484603
1114	13	0.22948402576285076
1114	14	0.9806684658778124
1114	15	0.4573608033108414
1114	16	0.7112359492731031
1114	17	1.150020318995729
1114	18	1.3549650477884327
1114	19	0.7500433233518038
1114	20	0.46274259413623753
1114	21	0.4497696016796837
1114	22	0.5509143432470296
1114	23	2.073238870565021
1114	24	1.0931039611767446
1114	25	1.006131264710369
1115	1	0.13500221765303166
1115	2	0.4455237501288245
1115	3	0.14141650199002243
1115	4	0.3940916133003114
1115	5	0.24283141664395202
1115	6	-0.03617629050587823
1115	7	-0.25610877950331457
1115	8	-0.1988408836040776
1115	9	-0.19566528908919167
1115	10	0.03594186222372997
1115	11	0.05254175501024605
1115	12	-0.1310927847553529
1115	13	-0.22901993014466626
1115	14	0.6068551535046609
1115	15	-0.10581152808170913
1115	16	0.3785633980855252
1115	17	0.8229811941910015
1115	18	1.361809643917681
1115	19	0.5757996253919231
1115	20	-0.01367461632229805
1115	21	0.15116705315350742
1115	22	0.38401044517776484
1115	23	1.9387125453750897
1115	24	0.8162987605055818
1115	25	0.539017985076768
1116	1	0.21040180032497346
1116	2	0.2896187446229028
1116	3	0.26664595368932
1116	4	0.8608891287781262
1116	5	0.3329149907993312
1116	6	0.07810108971104768
1116	7	-0.08151742023351005
1116	8	-0.017613827818522054
1116	9	-5.424075473428047E-4
1116	10	0.41422089842187404
1116	11	0.03129055663296043
1116	12	0.053817575629999226
1116	13	0.07386128148566229
1116	14	0.6554929514899399
1116	15	0.15800827878062051
1116	16	0.5280883965682079
1116	17	0.7112617026102583
1116	18	1.3009505761439897
1116	19	0.4553175134899661
1116	20	0.26833163121539627
1116	21	0.30231417023728113
1116	22	0.4796538325582773
1116	23	1.9971887705328868
1116	24	0.8312122227981433
1116	25	1.030102868331774
1201	1	1.0704801014035858
1201	2	1.3866024783044253
1201	3	1.1595725756653508
1201	4	1.7154901050541653
1201	5	1.4640084788282124
1201	6	1.0365409859104133
1201	7	1.0167511887749967
1201	8	1.0603746924338324
1201	9	1.2702677932114952
1201	10	1.4982183531549609
1201	11	1.1671740015366083
1201	12	1.3545359649750992
1201	13	1.197744829533569
1201	14	2.106017958372732
1201	15	1.571811064352822
1201	16	1.8779112671406537
1201	17	2.257055539636596
1201	18	2.812675551875138
1201	19	1.9446685199148632
1201	20	1.766691871916592
1201	21	1.6239695418261917
1201	22	1.958894357416689
1201	23	3.511624148180225
1201	24	2.498975187914283
1201	25	2.5127927351636785
1202	1	1.4274141048600555
1202	2	1.7366515365290063
1202	3	1.4389103099896197
1202	4	2.0544516991818327
1202	5	1.8817164622909104
1202	6	1.5244631970724853
1202	7	1.3149361362460539
1202	8	1.3441500586911346
1202	9	1.4974853369211516
1202	10	1.9693856539144434
1202	11	1.5716285290407654
1202	12	1.6891008429503955
1202	13	1.5780918197809584
1202	14	2.408410411955714
1202	15	1.9322044868866257
1202	16	2.2730836121441484
1202	17	2.701593791820301
1202	18	3.3060250072474204
1202	19	2.5528741695495754
1202	20	2.215712743035958
1202	21	2.2052338801610247
1202	22	2.6445395341382607
1202	23	4.131480719030684
1202	24	2.9300403417148715
1202	25	3.042423495688297
1203	1	0.8892216177744423
1203	2	1.121379456685485
1203	3	0.7903110823453048
1203	4	1.416151884747805
1203	5	1.3184033573207543
1203	6	0.9037846274350869
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2211	15	-0.43164315342368553
2211	16	-0.5608422623622005
2211	17	0.015768346007334344
2211	18	-5.2281813471703735
2211	19	-1.8577498278988998
2211	20	-0.9860409599555169
2211	21	-0.7994278085344177
2211	22	-0.39372908869599854
2211	23	-4.856778523204525
2211	24	-2.048062994964205
2211	25	-1.303654006821013
2212	1	0.08233344207154047
2212	2	0.3557538730080658
2212	3	0.12386718885547054
2212	4	0.58559861781346
2212	5	0.4601332705216947
2212	6	0.05907993857959326
2212	7	-0.04862449218359899
2212	8	-0.2001598306012511
2212	9	-0.25024624983688426
2212	10	-3.7884980935019286
2212	11	-1.2602373947178478
2212	12	-0.4900809867355065
2212	13	-0.225360555236783
2212	14	0.5932894465331486
2212	15	0.10067160630283212
2212	16	0.418257841466513
2212	17	0.7915931015753799
2212	18	1.4702377253935737
2212	19	0.731921209741003
2212	20	0.3129024875302946
2212	21	0.1689264220646436
2212	22	0.29170837955023643
2212	23	1.6328128917299622
2212	24	0.9085068092330957
2212	25	0.8867414378381042
2213	1	-1.3067278958892885
2213	2	-3.74685352154518
2213	3	-1.480196096904347
2213	4	-3.093333988341584
2213	5	-1.0329051188256138
2213	6	-0.7362524267566641
2213	7	-0.6202942871334898
2213	8	-0.5399245010690734
2213	9	-0.334558499721787
2213	10	-0.37455964394992236
2213	11	-0.5178918905920611
2213	12	-0.34440421512378805
2213	13	-0.737189184340437
2213	14	-5.813745826335783
2213	15	-2.3173552733231872
2213	16	-1.4716353837344585
2213	17	-0.3813567514543337
2213	18	0.2935521709791544
2213	19	-0.3864139533428954
2213	20	-0.5107728320858129
2213	21	-0.5785952693331708
2213	22	-0.44937016987073264
2213	23	-5.186323702100829
2213	24	-2.6621589838752335
2213	25	-1.8479024690925208
2214	1	-0.17539567586338833
2214	2	0.03204532652243586
2214	3	-0.08355612689548764
2214	4	0.02265401520282495
2214	5	-0.08087219673441126
2214	6	-0.19793533862361556
2214	7	-0.36528985662432234
2214	8	-0.5027862691830407
2214	9	-0.3675027434679521
2214	10	0.11889410835053127
2214	11	-0.3433898116608347
2214	12	-0.34528520483137726
2214	13	-0.2638973160288032
2214	14	0.3342512427119999
2214	15	-0.183555600499254
2214	16	0.3289197931450971
2214	17	0.57846568737056
2214	18	0.9975982482744996
2214	19	-0.0393898922700302
2214	20	0.03243894625240529
2214	21	-0.21586198598507167
2214	22	0.2581688526907797
2214	23	1.7647825619575173
2214	24	0.6870585200440615
2214	25	0.510346457361031
2215	1	-2.4679669534038133
2215	2	-4.369984095054136
2215	3	-2.4364535623383645
2215	4	-3.817265919945939
2215	5	-5.155765709515297
2215	6	-2.491533454327641
2215	7	-1.772927846816053
2215	8	-2.0651666674766673
2215	9	-1.7031852689273848
2215	10	-3.278242864108641
2215	11	-2.0107487996978435
2215	12	-1.7085639958650134
2215	13	-1.6158364067899171
2215	14	-0.9430720964822257
2215	15	-1.5520460202756539
2215	16	-2.4626126397272614
2215	17	-6.685208915407536
2215	18	-3.241322246103394
2215	19	-2.370495602692479
2215	20	-2.014840573229556
2215	21	-2.1045111655943174
2215	22	-1.7921121317458883
2215	23	-6.463772773523555
2215	24	-3.7966177910420527
2215	25	-3.4649310218209806
2216	1	-1.0164197248392988
2216	2	-0.5580243038512762
2216	3	-0.7615509118678752
2216	4	-3.0868071864945676
2216	5	-1.2398029974309779
2216	6	-1.0823877409506046
2216	7	-1.109596301454573
2216	8	-1.0610404081827731
2216	9	-0.9953177180848982
2216	10	-0.6406253603388663
2216	11	-1.2139395383200906
2216	12	-2.1522757937893857
2216	13	-1.467317337990977
2216	14	-7.2452525518929
2216	15	-3.4955206350310135
2216	16	-1.916547048058895
2216	17	-1.1350999757064955
2216	18	-0.30348456863136164
2216	19	-0.8391162219636499
2216	20	-1.3932475694210211
2216	21	-1.6133921642003344
2216	22	-1.073520942807896
2216	23	-0.005691298968926617
2216	24	-0.6637677639060158
2216	25	-0.4705378167469405
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	102914.0
3	2.0
4	844.0
5	5208.0
6	6025.0
7	5430.0
8	4953.0
9	4838.0
10	4489.0
11	4374.0
12	4381.0
13	4271.0
14	4367.0
15	4532.0
16	4581.0
17	4927.0
18	5623.0
19	6600.0
20	8181.0
21	10170.0
22	12512.0
23	15118.0
24	17971.0
25	21014.0
26	24207.0
27	27814.0
28	31553.0
29	37495.0
30	44442.0
31	50451.0
32	65431.0
33	85822.0
34	112357.0
35	148024.0
36	208890.0
37	293286.0
38	716536.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.587314859677505	18.504517492678858	12.813696314585862	25.094471333057776
2	13.61930442386724	19.82754287242649	42.835579110901605	23.71757359280466
3	19.14031326771715	24.529346858669154	28.867658543593887	27.462681330019805
4	10.441342424443581	16.714445571876986	40.002370769362074	32.84184123431736
5	12.38009345005581	38.008775997731554	36.500478440766756	13.110652111445878
6	31.86561648232496	36.990693702923885	18.58272524827934	12.560964566471824
7	28.399714398957727	30.992691192450728	22.127443301745274	18.48015110684627
8	24.928383472014794	37.300239432946924	20.48483986440035	17.286537230637936
9	26.22737841907818	15.734919262150507	20.058149458902786	37.97955285986853
10	13.504049057179952	29.84812834833177	35.46692424341572	21.180898351072557
11	34.50827393914223	22.927603802935028	23.736151825349076	18.827970432573665
12	22.59279909199264	25.121394781233374	32.286081108117926	19.99972501865606
13	28.671013719100262	21.84047765111254	25.772689044157314	23.715819585629884
14	20.24216810576163	21.91901558698196	29.01157597492125	28.827240332335162
15	23.669314596327034	30.240067893200962	23.281109617650337	22.809507892821667
16	21.86909276763926	28.549578436666884	28.124814797079235	21.456513998614625
17	21.679665182138052	28.385694852796494	28.342643048864268	21.591996916201182
18	21.092104346144055	27.907278846641624	30.15281742459046	20.84779938262386
19	22.95851669702115	27.491537334119183	27.9885574420487	21.56138852681097
20	22.964293317187863	27.60752281598762	28.474172382615425	20.954011484209094
21	22.906044828523054	27.115884382561134	27.64360788389514	22.334462905020676
22	22.314583341236453	27.940055157449102	27.795902264338473	21.949459236975976
23	21.12671581991892	27.28180934543989	29.41945425665584	22.17202057798535
24	22.127244292235275	27.852791690416417	28.167948125800812	21.85201589154749
25	22.439821448723407	27.230160258021197	27.935716609118938	22.394301684136455
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	165.0
1	165.0
2	281.0
3	397.0
4	397.0
5	397.0
6	796.0
7	1195.0
8	1195.0
9	1195.0
10	2155.0
11	3115.0
12	3115.0
13	3115.0
14	6071.5
15	9028.0
16	9028.0
17	9028.0
18	16445.5
19	23863.0
20	23863.0
21	23863.0
22	38315.5
23	52768.0
24	52768.0
25	52768.0
26	75149.5
27	97531.0
28	97531.0
29	97531.0
30	126027.0
31	154523.0
32	154523.0
33	154523.0
34	179924.0
35	205325.0
36	205325.0
37	205325.0
38	226297.0
39	247269.0
40	247269.0
41	247269.0
42	262538.0
43	277807.0
44	277807.0
45	277807.0
46	288753.5
47	299700.0
48	299700.0
49	299700.0
50	282404.5
51	265109.0
52	265109.0
53	265109.0
54	229932.0
55	194755.0
56	194755.0
57	194755.0
58	163431.5
59	132108.0
60	132108.0
61	132108.0
62	104431.0
63	76754.0
64	76754.0
65	76754.0
66	58214.0
67	39674.0
68	39674.0
69	39674.0
70	28705.5
71	17737.0
72	17737.0
73	17737.0
74	12342.5
75	6948.0
76	6948.0
77	6948.0
78	4772.0
79	2596.0
80	2596.0
81	2596.0
82	1723.0
83	850.0
84	850.0
85	850.0
86	572.0
87	294.0
88	294.0
89	294.0
90	189.0
91	84.0
92	84.0
93	84.0
94	54.5
95	25.0
96	25.0
97	25.0
98	19.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1128158309999891
2	0.04432050503571
3	0.033275929984030395
4	0.029057186723946773
5	0.03313372515503882
6	0.01971906962016616
7	0.018818439036552802
8	0.022894977467644847
9	0.027729941653358667
10	0.035409002418904136
11	0.019908676058821607
12	0.018913242255880525
13	0.014741900605460762
14	0.020714503423107242
15	0.020761905032771102
16	0.021804740445376045
17	0.025881278876468086
18	0.01820221811092261
19	0.03147466881680368
20	0.02256316619999782
21	0.027208523947056196
22	0.03607262495419819
23	0.027635138434030947
24	0.039769950507979344
25	0.031237660768484374
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2109633.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.71472575128416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.85542230836846	70.03370895074845
2	8.847413585645263	14.105382951757955
3	1.911060050402263	4.57018883436155
4	0.6234636005006339	1.987969197192649
5	0.25570678077476494	1.0191797951102062
6	0.13616233433816255	0.6512485883652773
7	0.08268952907978991	0.4614101193168802
8	0.05494257557739519	0.3503785875377008
9	0.03495738687136411	0.2507956656689099
>10	0.17608328458383837	2.432922587855929
>50	0.011345555760017174	0.6374433964134039
>100	0.008892375799192767	1.4969105791839383
>500	7.802651575595349E-4	0.43620372794604634
>1k	0.001020346744500638	1.2988986296234186
>5k	6.002039673533165E-5	0.26735838891762426
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	5588	0.2648801948016551	No Hit
CCCATGTACTCTGCGTTGATACCAC	4528	0.21463448855796244	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2166	0.10267188653192284	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.740160966386096E-5	0.0
5	0.0	0.0	0.0	4.740160966386096E-5	0.0
6	0.0	0.0	0.0	4.740160966386096E-5	0.0
7	0.0	0.0	0.0	4.740160966386096E-5	0.0
8	0.0	0.0	0.0	4.740160966386096E-5	0.0
9	0.0	0.0	0.0	1.422048289915829E-4	0.0
10	0.0	0.0	0.0	1.422048289915829E-4	0.0
11	0.0	0.0	0.0	1.422048289915829E-4	0.0
12	0.0	0.0	0.0	1.422048289915829E-4	0.0
13	0.0	0.0	0.0	1.422048289915829E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	715	0.0	12.225393	4
TGTAGGA	1245	0.0	12.132984	2
GTAGGAC	1120	0.0	12.129631	3
GACCGTC	55	0.0030709351	12.089929	7
TCCAACG	160	4.5474735E-11	11.2819395	18
GTCCTAC	1050	0.0	11.138495	1
CGGGGAC	60	0.005876237	11.083486	3
AGGACCT	970	0.0	10.9702635	5
CTAGGAC	360	0.0	10.819592	3
TGGCGAG	875	0.0	10.640651	18
GTCTAGG	225	0.0	10.56497	1
TGTAGAA	715	0.0	10.364024	2
GTCCTAG	370	0.0	10.279429	1
ACCGTGG	130	3.8469807E-7	10.229939	8
GGACCTG	930	0.0	10.214952	6
TTGGACA	270	0.0	10.205054	4
CTGTAGG	1370	0.0	10.202522	1
ATTGGAC	205	3.6379788E-12	10.195262	3
TAGGCGT	75	0.0026453366	10.134674	4
GGCGAGG	395	0.0	10.101884	19
>>END_MODULE