FastQCFastQC Report
Thu 26 May 2016
SRR1513047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513047_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109633
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC66190.31375125436509577No Hit
CCCATGTACTCTGCGTTGATACCAC58540.2774890229722421No Hit
GTATCAACGCAGAGTACTTTTTTTT27210.1289797798953657No Hit
GAGTACATGGGAAGCAGTGGTATCA22210.10527897506343521No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC508.828006E-515.1773210
TCCAACG2050.014.8131118
TGGTCGG456.84075E-414.75572710
TAGGACC6800.013.5785674
CCGGTTT1750.013.56178612
CGGTTTC1950.013.14450113
GTAGGAC12100.012.665773
CCAACGT1052.035631E-812.64836519
AGGACGT7450.012.521635
GCCGGTT1900.012.48430811
CCAACGA1405.4569682E-1112.19663819
GTCCTAC12450.012.1672081
TGTAGGA13750.012.1217162
ACCGTGC802.9045934E-511.8600928
TAGGACG8250.011.7689394
GGACGTG7800.011.7156856
AAGGCGT752.0363486E-411.4227936
CTAGTAC1751.8189894E-1211.4227923
TGCCGGT2100.011.29264810
TTAGGAC6350.011.0933
Produced by FastQC (version 0.11.5)