##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513047_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2109633 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.495909952110154 31.0 31.0 34.0 27.0 34.0 2 30.651753172234223 31.0 31.0 34.0 27.0 34.0 3 30.650535898898056 33.0 31.0 34.0 27.0 34.0 4 33.89101895922182 37.0 35.0 37.0 30.0 37.0 5 33.8062132133883 37.0 35.0 37.0 28.0 37.0 6 33.76286728544728 37.0 35.0 37.0 29.0 37.0 7 33.682920678620405 37.0 35.0 37.0 28.0 37.0 8 33.677482765959766 37.0 35.0 37.0 28.0 37.0 9 35.234511879554404 39.0 35.0 39.0 28.0 39.0 10 35.13998548562712 39.0 35.0 39.0 28.0 39.0 11 35.15215490087613 39.0 35.0 39.0 28.0 39.0 12 35.06823319506284 39.0 35.0 39.0 27.0 39.0 13 35.03342856316715 39.0 35.0 39.0 27.0 39.0 14 36.20004522113562 40.0 36.0 41.0 27.0 41.0 15 36.19281600164578 40.0 37.0 41.0 27.0 41.0 16 36.157901398015674 40.0 36.0 41.0 27.0 41.0 17 36.06859297328019 40.0 36.0 41.0 27.0 41.0 18 35.99208819733101 40.0 36.0 41.0 27.0 41.0 19 36.07013968780352 40.0 37.0 41.0 27.0 41.0 20 35.96421936896133 40.0 37.0 41.0 27.0 41.0 21 35.987550441237886 40.0 37.0 41.0 27.0 41.0 22 35.95837712057026 40.0 37.0 41.0 27.0 41.0 23 35.885317493611446 40.0 37.0 41.0 27.0 41.0 24 35.76103947937864 40.0 36.0 41.0 27.0 41.0 25 35.73567772214409 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6840058713942625 1101 2 0.5414377978162292 1101 3 0.6538714886002985 1101 4 0.9732602284408856 1101 5 0.8048748299553807 1101 6 0.7125492484948808 1101 7 0.7063782289171527 1101 8 0.5907169290999903 1101 9 0.6960546200192965 1101 10 0.6559167796287753 1101 11 0.7075162018292716 1101 12 0.6599073664939397 1101 13 0.735726527134581 1101 14 0.7599218629253599 1101 15 0.7651069151360232 1101 16 0.7546259035089662 1101 17 0.838111264596769 1101 18 0.7385392390838277 1101 19 0.7805868705126144 1101 20 0.9195955240334897 1101 21 0.8887233896152935 1101 22 0.870527963058116 1101 23 0.7798612366202846 1101 24 0.9151185124226018 1101 25 0.8705210168453306 1102 1 0.5568757246562761 1102 2 0.5722013919785169 1102 3 0.5524039073941545 1102 4 0.7450624982823726 1102 5 0.5105931173730198 1102 6 0.5803465581222582 1102 7 0.5019928480611782 1102 8 0.4708177438413301 1102 9 0.4778889430300879 1102 10 0.42980721103554487 1102 11 0.49728465303562785 1102 12 0.4812661813168049 1102 13 0.5689369119789518 1102 14 0.6337541668230244 1102 15 0.603117676022606 1102 16 0.5878521916372179 1102 17 0.5928612886278373 1102 18 0.6007281248021243 1102 19 0.6264575778212347 1102 20 0.7402582178987984 1102 21 0.65050950957135 1102 22 0.747777157954367 1102 23 0.68541314347938 1102 24 0.7866692990060713 1102 25 0.6954906823378693 1103 1 0.14954991519561034 1103 2 0.2529750689827317 1103 3 0.4890280969599381 1103 4 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-0.9033080265673092 2215 22 -1.0678071650013052 2215 23 -0.9922962599911074 2215 24 -0.8470706259098719 2215 25 -0.9641774138933528 2216 1 -1.2662243729034373 2216 2 -0.9226326970914478 2216 3 -2.3168925498810644 2216 4 -0.9416233562078062 2216 5 -1.0773356780832728 2216 6 -1.028534346644122 2216 7 -1.0953326869776632 2216 8 -1.0334811497256737 2216 9 -1.1692503858498569 2216 10 -1.1523663177629402 2216 11 -1.2131944807465018 2216 12 -1.1400581782517705 2216 13 -1.2101046583974693 2216 14 -1.413980135841122 2216 15 -1.4512429621453933 2216 16 -1.4084034277697128 2216 17 -1.4601634405440294 2216 18 -1.4173625571110833 2216 19 -1.3221442283839124 2216 20 -1.335226312458694 2216 21 -1.473824291573223 2216 22 -1.5791021629522533 2216 23 -1.6402412790654495 2216 24 -1.603349413443432 2216 25 -1.674815134403289 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57435.0 3 1.0 4 966.0 5 7429.0 6 8314.0 7 6493.0 8 5041.0 9 4546.0 10 3966.0 11 3741.0 12 3397.0 13 3262.0 14 3166.0 15 3140.0 16 3181.0 17 3419.0 18 3828.0 19 4505.0 20 5643.0 21 7268.0 22 9278.0 23 11278.0 24 13098.0 25 14679.0 26 16091.0 27 17882.0 28 19881.0 29 23664.0 30 30170.0 31 37832.0 32 48248.0 33 63305.0 34 84768.0 35 115489.0 36 169588.0 37 282681.0 38 1012960.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.11627025643388 19.537168119318665 14.003444953172098 27.343116671075357 2 13.18994222614338 20.299541793550958 40.727058845851 25.783457134454657 3 16.781023049980732 24.558252549140065 29.423743371477407 29.236981029401797 4 9.9633917368566 17.521246586491586 38.32443842127991 34.19092325537191 5 12.552068961920556 38.13064722442683 36.05206090207669 13.265222911575927 6 32.007819770373935 37.553928630560094 17.995748511312883 12.442503087753085 7 28.00317972861386 31.029941970426815 22.210508652293736 18.756369648665586 8 24.727935524832773 37.83629615826137 20.113306656766085 17.322461660139773 9 25.60719151077015 15.468215887575267 19.919333539124796 39.00525906252979 10 13.457014205271314 30.384121116683076 34.89921401006556 21.259650667980047 11 34.36111674684151 22.88017004526071 23.670762947274213 19.08795026062356 12 22.32682279321134 25.466530767042762 32.021617521570114 20.185028918175785 13 28.497441503487654 21.712092581829697 25.88594519308898 23.904520721593673 14 20.330784811638665 22.07738094955937 28.112016132052265 29.479818106749704 15 23.387112065042622 30.418196159330158 23.209261141279615 22.9854306343476 16 21.864385165056312 28.90638962989441 27.51061809420913 21.718607110840153 17 21.723350052842118 28.716858698419667 27.67191705707565 21.88787419166256 18 21.41765501644035 28.348357008824472 28.987471943262307 21.246516031472876 19 22.701564722617356 27.650877192982453 27.870602181128497 21.77695590327169 20 22.858952244300312 27.794502645720844 28.186371752811983 21.16017335716686 21 22.660813515905375 27.127799005798636 27.63091969077086 22.58046778752513 22 22.146079436565508 28.06516583690149 27.461553739441886 22.32720098709112 23 21.832264425515373 27.779231429207968 27.559382450951116 22.829121694325547 24 22.06827737565355 27.893516872626954 28.084640434578574 21.95356531714092 25 22.30193592914028 27.378885332188112 27.57792469116666 22.741254047504945 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 76.0 1 76.0 2 182.0 3 288.0 4 288.0 5 288.0 6 786.5 7 1285.0 8 1285.0 9 1285.0 10 2108.0 11 2931.0 12 2931.0 13 2931.0 14 5675.0 15 8419.0 16 8419.0 17 8419.0 18 15918.5 19 23418.0 20 23418.0 21 23418.0 22 37584.0 23 51750.0 24 51750.0 25 51750.0 26 73944.0 27 96138.0 28 96138.0 29 96138.0 30 124411.0 31 152684.0 32 152684.0 33 152684.0 34 177295.5 35 201907.0 36 201907.0 37 201907.0 38 222685.5 39 243464.0 40 243464.0 41 243464.0 42 258368.0 43 273272.0 44 273272.0 45 273272.0 46 288088.0 47 302904.0 48 302904.0 49 302904.0 50 286890.0 51 270876.0 52 270876.0 53 270876.0 54 234183.5 55 197491.0 56 197491.0 57 197491.0 58 165951.0 59 134411.0 60 134411.0 61 134411.0 62 106808.5 63 79206.0 64 79206.0 65 79206.0 66 60069.5 67 40933.0 68 40933.0 69 40933.0 70 29381.5 71 17830.0 72 17830.0 73 17830.0 74 12289.0 75 6748.0 76 6748.0 77 6748.0 78 4612.5 79 2477.0 80 2477.0 81 2477.0 82 1634.0 83 791.0 84 791.0 85 791.0 86 529.5 87 268.0 88 268.0 89 268.0 90 160.5 91 53.0 92 53.0 93 53.0 94 32.5 95 12.0 96 12.0 97 12.0 98 6.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18695194851426766 2 0.06697847445503555 3 0.0 4 0.0 5 0.019482061571846856 6 0.36883192479450216 7 9.006305836133584E-4 8 0.031569472036131405 9 0.033275929984030395 10 0.003033703018487102 11 0.003649923944117294 12 0.011044575051679606 13 0.009053707445797445 14 0.0020856708252098826 15 4.740160966386097E-5 16 0.012419221731931572 17 0.024648837025207704 18 0.06659926157772465 19 0.03000521891722399 20 0.0 21 0.0 22 0.0 23 1.422048289915829E-4 24 1.422048289915829E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2109633.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.62797161635994 #Duplication Level Percentage of deduplicated Percentage of total 1 84.88290144724336 64.19521661366399 2 10.790173953140554 16.320779389274005 3 2.473425893859333 5.611805498878901 4 0.8391073254755087 2.5383993997656593 5 0.3654524382010875 1.381921331170069 6 0.172997023697499 0.7850048398745523 7 0.11353406376798177 0.6010445666494448 8 0.06970378968496295 0.42172449822776803 9 0.04994874399920945 0.3399769974100537 >10 0.2159522074809678 2.822693819201299 >50 0.014800797516142786 0.7761857761303833 >100 0.009490562307365797 1.5406472444208166 >500 0.001130289131734653 0.5599511468518081 >1k 0.001255876813038094 1.512309750783751 >5k 1.255876813038094E-4 0.5923391276974636 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 6619 0.31375125436509577 No Hit CCCATGTACTCTGCGTTGATACCAC 5854 0.2774890229722421 No Hit GTATCAACGCAGAGTACTTTTTTTT 2721 0.1289797798953657 No Hit GAGTACATGGGAAGCAGTGGTATCA 2221 0.10527897506343521 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGC 50 8.828006E-5 15.17732 10 TCCAACG 205 0.0 14.81311 18 TGGTCGG 45 6.84075E-4 14.755727 10 TAGGACC 680 0.0 13.578567 4 CCGGTTT 175 0.0 13.561786 12 CGGTTTC 195 0.0 13.144501 13 GTAGGAC 1210 0.0 12.66577 3 CCAACGT 105 2.035631E-8 12.648365 19 AGGACGT 745 0.0 12.52163 5 GCCGGTT 190 0.0 12.484308 11 CCAACGA 140 5.4569682E-11 12.196638 19 GTCCTAC 1245 0.0 12.167208 1 TGTAGGA 1375 0.0 12.121716 2 ACCGTGC 80 2.9045934E-5 11.860092 8 TAGGACG 825 0.0 11.768939 4 GGACGTG 780 0.0 11.715685 6 AAGGCGT 75 2.0363486E-4 11.422793 6 CTAGTAC 175 1.8189894E-12 11.422792 3 TGCCGGT 210 0.0 11.292648 10 TTAGGAC 635 0.0 11.093 3 >>END_MODULE