FastQCFastQC Report
Thu 26 May 2016
SRR1513046_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513046_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742479
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC52320.30026186829224344No Hit
CCCATGTACTCTGCGTTGATACCAC41320.2371334173898222No Hit
GAGTACATGGGAAGCAGTGGTATCA21370.12264136325315829No Hit
GTATCAACGCAGAGTACTTTTTTTT17510.10048901593649048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4900.012.6027084
GTAGGAC9750.011.6912493
GCGTTTA1302.6211637E-911.69057713
TGGCGGG907.4598865E-611.61139218
GTCCTAA3850.011.6106831
GACGTGG3400.011.4551737
GTCCTAC9950.011.1836461
GGACGTG5550.010.9542766
TGTAGGA10600.010.9339122
TCCAACG1408.347342E-910.85740518
CGTTTAT1408.36917E-910.855535514
TGCGTTT1408.36917E-910.855535512
TCCTACA10950.010.6711852
AGGACGT6400.010.5396195
TCGAACT1002.3981895E-510.45025319
AGGACCT7300.010.41150955
GACCGTG1106.0498824E-610.3629947
GTACTAG1606.0554157E-910.1053091
CTGTAGG10450.010.1024651
GAGGCGA951.6451093E-49.999386
Produced by FastQC (version 0.11.5)