FastQCFastQC Report
Thu 26 May 2016
SRR1513046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513046_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742479
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC60490.34714909046249626No Hit
CCCATGTACTCTGCGTTGATACCAC56760.3257428066564934No Hit
GTATCAACGCAGAGTACTTTTTTTT27450.1575341797519511No Hit
GAGTACATGGGAAGCAGTGGTATCA23070.1323975783926234No Hit
TATCAACGCAGAGTACTTTTTTTTT20330.11667285516783847No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCT551.9846727E-413.79812057
TAGGACC4700.012.7617114
TCCAACG1001.4632315E-712.33667418
CCGTGAT853.9791776E-612.28437917
TGTAGGA9300.011.9865592
GTAGGAC8350.011.9720643
TGGCGAG8150.011.76030218
ATGGCGA8550.011.54640317
GGCGAGA6000.011.54055919
TTAGTAC1354.5693014E-911.2837233
GGACCGC600.005781958311.1074156
TAGGACT2600.010.9853544
TATGGCG8600.010.92738316
CGAGAAC700.00146479110.8807333
GTCCTAG2150.010.6399531
TAGGACA3850.010.6334444
CTGTAGG9600.010.6237911
AGGACCT8450.010.4783395
TGGAATA11900.010.442511
CTAGGAC2300.010.3485233
Produced by FastQC (version 0.11.5)