FastQCFastQC Report
Thu 26 May 2016
SRR1513045_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513045_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977774
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5600.013.4038554
GCCGGTT905.418897E-712.66445811
GTAGGAC8150.012.4736883
ATAGGAC2600.012.0589453
GTCCTAC10000.011.7952461
GTGTAGG4250.011.1909351
TGTAGGA10650.011.1513922
CTGTAGG10350.010.9368421
TAGGACA4050.010.7917894
AGGACCT8800.010.6883395
TCCTACA11650.010.6835132
TGCGCCA1252.2460881E-710.638144512
TTGGACT2100.010.4063684
TATGGCG8050.010.38433316
CCGGTTT1201.5274272E-610.28987212
CTACACT4500.010.1348984
GGACCTG8250.010.1323356
TGGCGAG7800.010.10900118
TAGGACG5200.010.0496294
CCTACAG12300.09.8872053
Produced by FastQC (version 0.11.5)