##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513045_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1977774
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.0361345634031	31.0	30.0	34.0	23.0	34.0
2	28.745790975106356	31.0	30.0	34.0	16.0	34.0
3	29.100399236717642	31.0	30.0	34.0	22.0	34.0
4	31.96147537585184	35.0	32.0	37.0	18.0	37.0
5	32.16981060525621	35.0	32.0	37.0	19.0	37.0
6	32.672798813211216	36.0	33.0	37.0	25.0	37.0
7	32.64572645812919	36.0	33.0	37.0	25.0	37.0
8	32.625537093722535	37.0	33.0	37.0	25.0	37.0
9	33.99700825271239	39.0	34.0	39.0	25.0	39.0
10	33.50202854320059	38.0	34.0	39.0	17.0	39.0
11	33.873105319414655	39.0	34.0	39.0	23.0	39.0
12	33.7070620809051	39.0	34.0	39.0	21.0	39.0
13	33.80089888935743	39.0	34.0	39.0	22.0	39.0
14	34.087363874739985	39.0	33.0	41.0	11.0	41.0
15	34.63691351994717	40.0	34.0	41.0	18.0	41.0
16	34.23243859005124	39.0	33.0	41.0	17.0	41.0
17	33.76338752557168	39.0	33.0	41.0	10.0	41.0
18	33.18381574436715	39.0	32.0	41.0	10.0	41.0
19	34.06645147524439	39.0	33.0	41.0	18.0	41.0
20	34.31755650544501	39.0	34.0	41.0	17.0	41.0
21	34.33869188289461	39.0	34.0	41.0	15.0	41.0
22	33.92436092293659	39.0	34.0	41.0	9.0	41.0
23	32.250777894744296	38.0	31.0	41.0	2.0	41.0
24	33.33729738584894	39.0	32.0	41.0	2.0	41.0
25	33.074171265270955	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8724385281520668
1101	2	1.2294399420610134
1101	3	0.9984067653392685
1101	4	1.0541992725269296
1101	5	1.2759327390600177
1101	6	0.9277928357500471
1101	7	0.9355825008773024
1101	8	0.9136748044997844
1101	9	0.9757389618977541
1101	10	1.423939959290614
1101	11	1.0094703329265542
1101	12	1.0258982593126547
1101	13	0.9777268829808037
1101	14	0.21909532469928905
1101	15	0.7050637658800554
1101	16	1.2660949348877324
1101	17	1.9631746550328018
1101	18	-4.097241265188583
1101	19	-0.5366376734153846
1101	20	0.4397852518099512
1101	21	0.9208071303129799
1101	22	1.4019119272395386
1101	23	3.29867839806154
1101	24	2.1509064936330162
1101	25	2.37969987728372
1102	1	1.1125864719733443
1102	2	1.4218917467999788
1102	3	1.192942206166947
1102	4	1.7734051457322977
1102	5	1.4268024788692202
1102	6	1.1141024978003458
1102	7	1.0967833860321647
1102	8	0.9984028498114554
1102	9	1.238309138897037
1102	10	1.5701341374235014
1102	11	1.24552717442851
1102	12	1.405087544918608
1102	13	1.2998956165329858
1102	14	2.1777153985014337
1102	15	1.5983105213610074
1102	16	1.8150235996086153
1102	17	1.4493453551933797
1102	18	1.857972103221364
1102	19	1.6345528942888663
1102	20	1.63672700371216
1102	21	1.7252564686425131
1102	22	1.9245736484112328
1102	23	3.572124227371333
1102	24	2.4470266070480093
1102	25	2.665522346509057
1103	1	0.523164136165466
1103	2	0.8296419394899317
1103	3	0.5967290707971848
1103	4	1.0030733133522745
1103	5	1.0387695390290475
1103	6	0.5915922548081767
1103	7	0.565849549676642
1103	8	0.4845575697011171
1103	9	0.5948687105168489
1103	10	0.8462791213998102
1103	11	0.5764670957298748
1103	12	0.7641216611816404
1103	13	0.6010821809725613
1103	14	1.402832026630108
1103	15	0.8432985543008442
1103	16	1.1794063832685069
1103	17	1.5698414811111974
1103	18	1.480865697801704
1103	19	0.8718637538888814
1103	20	0.9169874393942834
1103	21	0.9341668576733824
1103	22	1.1981568701872192
1103	23	2.741121428296246
1103	24	1.753866086176643
1103	25	1.7712442239176838
1104	1	0.5635359919784975
1104	2	0.9352965245562821
1104	3	0.7031734162121168
1104	4	1.266340792060923
1104	5	1.13383291791596
1104	6	0.6106381054716721
1104	7	0.44800690756929384
1104	8	0.6068119765143436
1104	9	0.7897439333332485
1104	10	1.1565385787180915
1104	11	0.6166359410457787
1104	12	0.6758962700150732
1104	13	0.5964995903605583
1104	14	1.327292315796278
1104	15	0.8395379086284365
1104	16	1.2065701780793603
1104	17	1.659210663127439
1104	18	2.2214603700140287
1104	19	1.3347301630788238
1104	20	0.730282151218411
1104	21	0.8771456653580714
1104	22	1.2759519781293918
1104	23	2.8189363421405744
1104	24	1.8152074409003518
1104	25	1.9267750220955406
1105	1	0.8029337128131537
1105	2	1.1797349206689312
1105	3	0.8173253804004688
1105	4	1.0756186493648912
1105	5	1.2037043962920393
1105	6	0.8267058170887935
1105	7	0.6165274791988153
1105	8	0.6688846219125892
1105	9	0.9083968849882353
1105	10	1.3493609750296542
1105	11	0.9344362481194892
1105	12	0.9167923959845581
1105	13	0.869731141517903
1105	14	1.523637446040638
1105	15	1.024024892184272
1105	16	1.4666899350489047
1105	17	1.9344907413433177
1105	18	1.571174408734798
1105	19	1.37296197175894
1105	20	1.2476611643556197
1105	21	1.1808697998595505
1105	22	1.5884271138616484
1105	23	3.0582270578229966
1105	24	2.0878213814072666
1105	25	2.121132120932785
1106	1	-0.6907557023445356
1106	2	-0.13455630480198977
1106	3	-0.349830311359387
1106	4	-2.8166865694052703
1106	5	-0.6266071001180826
1106	6	-0.580056540494013
1106	7	-0.5508528822482717
1106	8	-0.5645479609032407
1106	9	-0.46469413101320356
1106	10	-0.4071557514729989
1106	11	-0.3841685909011261
1106	12	-0.3296682508752511
1106	13	-0.4261466141719268
1106	14	0.22911504190609833
1106	15	-0.37770550885778675
1106	16	-1.5051915758025203
1106	17	-6.071320670121214
1106	18	-7.020954218772307
1106	19	-3.143221412484337
1106	20	-1.7737851239778095
1106	21	-1.3005504503460372
1106	22	-1.883696344687067
1106	23	-5.021012118614816
1106	24	-2.308360056143176
1106	25	-0.8285669805773637
1107	1	0.6406927140585061
1107	2	0.9230390848232943
1107	3	0.7085622304663346
1107	4	0.9137723418189552
1107	5	1.0979691936728955
1107	6	0.7175792097245193
1107	7	0.647805676735274
1107	8	0.6055952597593048
1107	9	0.5459805447721919
1107	10	1.0211085524934802
1107	11	0.7412464655206321
1107	12	0.7523927000221562
1107	13	0.6891180883706554
1107	14	1.4165212564924587
1107	15	0.8867866952832912
1107	16	-5.888675893014227
1107	17	-0.8897414399751398
1107	18	0.6254904900347
1107	19	0.7308011043979903
1107	20	0.6947277451528109
1107	21	0.7865532884197179
1107	22	0.9574028718022447
1107	23	2.7549469598894305
1107	24	1.8013017181225237
1107	25	1.3642516383374002
1108	1	0.43592859669112016
1108	2	0.7432757904372131
1108	3	0.49796028844631124
1108	4	1.0556035912556823
1108	5	0.7295178178370278
1108	6	0.35134400112708875
1108	7	0.32358611935351433
1108	8	0.34373642290982076
1108	9	0.46468628928965217
1108	10	0.9604406370538641
1108	11	0.4872063288571695
1108	12	0.41304874294454663
1108	13	0.4372799798569247
1108	14	1.1319792199564063
1108	15	0.8094476070170629
1108	16	0.9654961365224395
1108	17	1.0333220580246092
1108	18	0.7737296608149222
1108	19	0.6906988712314615
1108	20	0.4441697098601338
1108	21	0.5426044676035318
1108	22	0.9631098829014277
1108	23	2.5864252124059988
1108	24	1.3803699305909376
1108	25	1.6464540334558535
1109	1	0.5172261178715978
1109	2	0.8293080126321151
1109	3	0.6096434978796523
1109	4	0.7919045138803398
1109	5	0.5644835711681253
1109	6	0.554450380269266
1109	7	0.4895722239759266
1109	8	0.41421216064071587
1109	9	0.39020094555254303
1109	10	1.0144124037011366
1109	11	0.7580312268821459
1109	12	0.7060106221809832
1109	13	0.5632924217660005
1109	14	-0.4207109262373905
1109	15	0.34899916280211585
1109	16	0.16435193987798158
1109	17	1.2425166124354234
1109	18	1.9264378387609966
1109	19	1.1541351221569585
1109	20	0.8762738787466233
1109	21	0.8638286596580826
1109	22	0.7469151836901844
1109	23	-4.117026775321477
1109	24	-1.1773505280712726
1109	25	0.19067854516419658
1110	1	0.683121084089219
1110	2	1.0595017676900085
1110	3	0.8592930014109079
1110	4	1.2513177537128968
1110	5	0.911967310982007
1110	6	0.5086131929909428
1110	7	0.44316166433316084
1110	8	0.4421640634071977
1110	9	0.4671210777899617
1110	10	0.8869792388634181
1110	11	0.42896376150454785
1110	12	0.6758070865600132
1110	13	0.34287901944989585
1110	14	1.2916197872021016
1110	15	0.7938969953557162
1110	16	1.2335176631059284
1110	17	1.4610725491057508
1110	18	2.1292575856126845
1110	19	1.2326143602826463
1110	20	0.8911129911131965
1110	21	0.766160482613067
1110	22	0.9688582450379712
1110	23	2.3313779675154436
1110	24	1.4679788060984933
1110	25	1.8229481984432354
1111	1	0.574581539465715
1111	2	0.9537347070973432
1111	3	0.602565787930974
1111	4	1.2142444605066842
1111	5	1.0343639337866222
1111	6	0.5080122210189373
1111	7	0.39694691968615103
1111	8	0.2637595237984698
1111	9	0.37222092594437584
1111	10	0.6469870915638509
1111	11	0.4611317879259502
1111	12	0.570190936706986
1111	13	0.30546784644609204
1111	14	-0.4404401978136647
1111	15	0.15507981503608192
1111	16	0.5866180775094918
1111	17	1.1589626032867102
1111	18	1.833452503526587
1111	19	0.9530849346916526
1111	20	0.6365759865978973
1111	21	0.5867663424904421
1111	22	0.8607300323419764
1111	23	0.9058030042748939
1111	24	0.7050769394795466
1111	25	0.26606085932009194
1112	1	0.25436978728262005
1112	2	0.5788804263390368
1112	3	0.4568228446077711
1112	4	0.9089904705968621
1112	5	0.7681878264119391
1112	6	0.20194057577596425
1112	7	0.30376004953404845
1112	8	0.17439675378808772
1112	9	0.2026858196613901
1112	10	0.6115285393040963
1112	11	0.16769117810439838
1112	12	0.24751075651764154
1112	13	0.13849231263048267
1112	14	0.6984176766405383
1112	15	0.36098532508496106
1112	16	0.7414850289754682
1112	17	0.9124768529501068
1112	18	1.0069933885311713
1112	19	0.5520424902623091
1112	20	0.5082023699897462
1112	21	0.42971568773548796
1112	22	0.2934909526894458
1112	23	2.0513661000453425
1112	24	1.022965423417574
1112	25	1.0284781731870325
1113	1	0.4757465741366005
1113	2	0.8527398138522457
1113	3	0.4662432362838764
1113	4	0.6336644784111201
1113	5	0.7952493123055717
1113	6	0.37919972717993033
1113	7	0.2926206428542315
1113	8	0.18170155613638883
1113	9	0.36553163069584826
1113	10	0.7007342776033312
1113	11	0.3299673390833817
1113	12	0.3161737672143943
1113	13	0.11314915101642953
1113	14	0.7956391697650673
1113	15	0.35102310966823325
1113	16	0.7208995751519538
1113	17	1.171414921835435
1113	18	1.631366753735172
1113	19	0.7609994652537537
1113	20	0.5454184873070673
1113	21	0.4659903480669598
1113	22	0.6796198504950368
1113	23	0.6304521378267722
1113	24	0.5532057204210545
1113	25	1.0598106037110355
1114	1	-0.6017323417430624
1114	2	-3.362347775391349
1114	3	-1.318771314582719
1114	4	-0.16488648231836933
1114	5	0.1949005120274947
1114	6	-0.22494600389875785
1114	7	-0.315764240370882
1114	8	-0.21208010765945318
1114	9	-0.10488414706733096
1114	10	-0.09182137694165249
1114	11	-0.182955988994415
1114	12	-0.24492275268205077
1114	13	-0.28611509297169846
1114	14	0.4464055436976011
1114	15	-0.09667454711804169
1114	16	0.30230606279469185
1114	17	0.545911105373591
1114	18	0.5933495102855204
1114	19	0.0766031465800836
1114	20	-0.11031243609836139
1114	21	-0.24511218546108182
1114	22	-0.04479098962810468
1114	23	1.4716453889406935
1114	24	0.43997275059656005
1114	25	0.34534419801765637
1115	1	0.15077983131243755
1115	2	0.3533801865727
1115	3	0.14860321864325954
1115	4	0.2694028686961687
1115	5	0.21828373294665937
1115	6	-0.10352173731527614
1115	7	-0.2543941856113392
1115	8	-0.15195792601486602
1115	9	-0.17303892858395642
1115	10	-0.08886162591670654
1115	11	-0.0918013891391567
1115	12	-0.13701995537041256
1115	13	-0.36001459699940597
1115	14	0.5985549973607078
1115	15	-0.07582534669671759
1115	16	0.4651325288664836
1115	17	0.8594013047570712
1115	18	1.1829600828072913
1115	19	0.5604670074600975
1115	20	1.701977067369853E-4
1115	21	-5.776990730055331E-4
1115	22	0.3497815494896557
1115	23	2.0549952905070015
1115	24	0.7979992086741277
1115	25	0.47671044137657503
1116	1	0.17642559357199872
1116	2	0.16239607846168624
1116	3	0.20800591523465073
1116	4	0.9008978453736916
1116	5	0.24383225600886504
1116	6	0.06318032898692394
1116	7	-0.07086648807566576
1116	8	-0.1938098698062234
1116	9	-0.024647697082457398
1116	10	0.5039089603768048
1116	11	0.11366986609326801
1116	12	0.18259307654425072
1116	13	0.0828113901629095
1116	14	0.7297872437407662
1116	15	-0.05586056713806897
1116	16	0.4771262416101223
1116	17	0.703351015138864
1116	18	1.2561190953348955
1116	19	0.4863209336310348
1116	20	0.225073481159086
1116	21	0.2616628887963586
1116	22	0.4828239206573812
1116	23	1.9005970131604357
1116	24	0.7338838807022086
1116	25	1.0107044566421663
1201	1	1.0087784196050862
1201	2	1.3098149734772555
1201	3	1.1344617072526368
1201	4	1.6323476468378573
1201	5	1.4976868678807946
1201	6	0.9865789413723576
1201	7	0.9419395254904757
1201	8	0.9514776042428252
1201	9	1.2583941966519347
1201	10	1.5193479758632122
1201	11	1.1877496369399267
1201	12	1.4233276899423828
1201	13	1.2338827444255216
1201	14	2.174728996559523
1201	15	1.5449395180750471
1201	16	1.8037041300377723
1201	17	2.2786992452675463
1201	18	2.8697362233308468
1201	19	2.025244529775698
1201	20	1.6646483773900513
1201	21	1.6018378141603549
1201	22	2.0045287235732303
1201	23	3.5691877316473253
1201	24	2.366052151175964
1201	25	2.5453300365951463
1202	1	1.3681798362373456
1202	2	1.6378892061641643
1202	3	1.4243687636054183
1202	4	1.9879066656013507
1202	5	1.7559488225004642
1202	6	1.493370335237941
1202	7	1.2265161406803173
1202	8	1.356134764873815
1202	9	1.5274189318311144
1202	10	1.9869177170945918
1202	11	1.6926476276407456
1202	12	1.6388086055315085
1202	13	1.5805056289353345
1202	14	2.540905646726202
1202	15	1.9840468613412838
1202	16	2.2931581329753143
1202	17	2.6208837388950244
1202	18	3.3205698554833916
1202	19	2.4480147772309166
1202	20	2.2342953499606253
1202	21	2.1825192959134583
1202	22	2.4693027862769554
1202	23	4.001511849360824
1202	24	2.8489290756216548
1202	25	3.012639449582572
1203	1	0.9869604524558433
1203	2	1.3018212601253083
1203	3	1.04201897926999
1203	4	1.7805817994118271
1203	5	1.4669417714768187
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2211	15	-0.3848542023456787
2211	16	-0.48945965694387183
2211	17	0.04140349287148126
2211	18	-5.362813929728254
2211	19	-1.9121912724430885
2211	20	-1.0578314240078868
2211	21	-0.904748788501621
2211	22	-0.4907580760990058
2211	23	-5.239820385104792
2211	24	-2.3755676822721554
2211	25	-1.6529256272428938
2212	1	0.41898620663542374
2212	2	0.6640944457734221
2212	3	0.4342355914022953
2212	4	1.0251200720187512
2212	5	0.8143801047804615
2212	6	0.3630059219964892
2212	7	0.2786868427956293
2212	8	0.24944114726074673
2212	9	0.15423704171616492
2212	10	-3.5880761685892786
2212	11	-0.9163958055837327
2212	12	-0.16410900226028957
2212	13	0.0143415350769871
2212	14	0.8342969102076694
2212	15	0.5338353779362635
2212	16	0.8202253712705954
2212	17	1.2262909523494017
2212	18	1.7750445757834044
2212	19	1.0185583998793035
2212	20	0.6678339324942968
2212	21	0.45265218038405663
2212	22	0.6624144696560919
2212	23	1.975678264261532
2212	24	1.1676731711104864
2212	25	1.0645979489615485
2213	1	-1.5037280484560327
2213	2	-3.727569257362063
2213	3	-1.6114571316160848
2213	4	-3.5120142740893883
2213	5	-1.2849871642016772
2213	6	-1.0017979223536493
2213	7	-0.9250334086213243
2213	8	-0.8896011653941187
2213	9	-0.6632240840095562
2213	10	-0.8049563463847278
2213	11	-0.8894579231136817
2213	12	-0.7219360508542607
2213	13	-1.0446295871253497
2213	14	-5.843336738204471
2213	15	-2.6266891136983688
2213	16	-1.860092086569928
2213	17	-0.6610451055162514
2213	18	-0.04658239872913583
2213	19	-0.7264998263220335
2213	20	-0.8388013659134401
2213	21	-1.0261208602153644
2213	22	-0.9700611233033811
2213	23	-5.600419056733653
2213	24	-3.0451464898724936
2213	25	-2.214942639369884
2214	1	-0.18849213890544547
2214	2	0.06389377618079095
2214	3	-0.15817772069497238
2214	4	0.19564758641434565
2214	5	-0.12062048377561041
2214	6	-0.3560934831214624
2214	7	-0.4810181264593254
2214	8	-0.4598818210797404
2214	9	-0.48436831262261393
2214	10	-0.029331266405698386
2214	11	-0.49266386364498516
2214	12	-0.3856771398366732
2214	13	-0.3859854938999945
2214	14	0.0023064456167176672
2214	15	-0.25050186388981643
2214	16	0.21187680354380234
2214	17	0.48785147473094526
2214	18	0.9770268085969676
2214	19	0.008175590200721672
2214	20	-0.10322216130312256
2214	21	-0.2052454012510765
2214	22	0.22726534075911076
2214	23	1.6960288514963509
2214	24	0.7964154067747486
2214	25	0.529521809024537
2215	1	-2.53996256115828
2215	2	-4.532880137765499
2215	3	-2.491262423943329
2215	4	-3.985723868063168
2215	5	-5.167427005100606
2215	6	-2.496169261124475
2215	7	-1.8139640528546437
2215	8	-2.16774693563141
2215	9	-1.8155059003097023
2215	10	-3.290832174343599
2215	11	-2.1311443202274276
2215	12	-1.818551916008353
2215	13	-1.777059890541011
2215	14	-1.0670270542865197
2215	15	-1.6786535163707157
2215	16	-2.535613860344686
2215	17	-6.74246487054436
2215	18	-3.1714545317104488
2215	19	-2.2569838260584234
2215	20	-2.1140618300400433
2215	21	-1.9178009473224478
2215	22	-1.707301043285156
2215	23	-6.331998137351189
2215	24	-3.6574005421433142
2215	25	-3.3910376284515458
2216	1	-1.0391274953974552
2216	2	-0.48556042066414307
2216	3	-0.7880020566907469
2216	4	-3.202420016197248
2216	5	-1.429530079929041
2216	6	-1.0377692561161105
2216	7	-1.063790721930264
2216	8	-0.8790125771877335
2216	9	-0.9711541165244171
2216	10	-0.5001778391923537
2216	11	-1.1351914859901626
2216	12	-1.7674019447932352
2216	13	-1.200589496291741
2216	14	-6.972186227317032
2216	15	-3.4870893162099392
2216	16	-1.8448638283590384
2216	17	-0.9636343364734898
2216	18	-0.160910862060895
2216	19	-0.5902688392358044
2216	20	-1.1326325533336998
2216	21	-1.3056835043016548
2216	22	-0.8036250697861149
2216	23	0.33844940516768673
2216	24	-0.3984418926458204
2216	25	-0.41974438621052457
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	103888.0
3	2.0
4	935.0
5	5270.0
6	5934.0
7	5400.0
8	4970.0
9	4724.0
10	4451.0
11	4342.0
12	4130.0
13	4093.0
14	4216.0
15	4436.0
16	4522.0
17	4792.0
18	5415.0
19	6381.0
20	7912.0
21	9712.0
22	12037.0
23	14651.0
24	17311.0
25	20327.0
26	23172.0
27	26875.0
28	29397.0
29	35617.0
30	42266.0
31	47880.0
32	61646.0
33	81800.0
34	106238.0
35	138684.0
36	195555.0
37	270745.0
38	658048.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.48573331875483	17.754182206941795	12.390509397057572	25.3695750772458
2	14.189696408048302	19.07597083056801	42.27281188791945	24.461520873464234
3	19.980324518987597	22.899427438444576	28.527677180488396	28.59257086207943
4	10.78644740908284	15.726773341722483	38.99690312908889	34.48987612010579
5	12.830901190300967	37.40346159935217	36.16915596518675	13.596481245160119
6	33.463456128186884	36.124424296398786	17.554591645540047	12.857527929874282
7	29.48228246209273	30.512023165878677	20.81540777721756	19.19028659481104
8	25.45682405187294	37.23929635112247	19.473947462515675	17.82993213448892
9	26.019804604148156	15.607695058256125	19.34997524298041	39.02252509461531
10	13.95080998731455	29.363329941104706	34.65490311916434	22.030956952416407
11	35.7345687784437	22.28049263390633	22.98825056097212	18.996688026677848
12	22.863163793007534	24.52990968885185	31.55463829520387	21.052288222936745
13	29.301910904042145	21.021208723800104	25.377495460041537	24.299384912116214
14	20.575879028485453	21.196717787920548	28.135941233737498	30.091461949856495
15	23.738121608431573	29.76442981191809	22.771110537947635	23.7263380417027
16	22.71904081094927	28.172183092715102	26.990341049603582	22.118435046732042
17	21.939393510269582	27.937784201973752	27.861870570545815	22.26095171721085
18	21.587344934484346	27.537106994573655	29.305903236083928	21.56964483485807
19	23.422660639690942	26.99175716487339	27.268322314730938	22.317259880704725
20	23.274767323129925	27.17111554923272	27.81198525473711	21.742131872900245
21	23.34282477417789	26.55931054713183	27.033966882795035	23.063897795895247
22	22.77274697616592	27.227834700610963	27.51199645126586	22.48742187195726
23	21.809414563684253	26.606824223444157	28.987371990175966	22.59638922269562
24	22.822832696090607	26.965306647877323	27.690089109446753	22.521771546585317
25	22.948914294731605	26.72566747658749	27.626105885851743	22.699312342829167
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	148.0
1	148.0
2	255.5
3	363.0
4	363.0
5	363.0
6	692.5
7	1022.0
8	1022.0
9	1022.0
10	1849.5
11	2677.0
12	2677.0
13	2677.0
14	4980.5
15	7284.0
16	7284.0
17	7284.0
18	13337.5
19	19391.0
20	19391.0
21	19391.0
22	31368.0
23	43345.0
24	43345.0
25	43345.0
26	61786.5
27	80228.0
28	80228.0
29	80228.0
30	105069.0
31	129910.0
32	129910.0
33	129910.0
34	151819.5
35	173729.0
36	173729.0
37	173729.0
38	194417.5
39	215106.0
40	215106.0
41	215106.0
42	231662.5
43	248219.0
44	248219.0
45	248219.0
46	258795.0
47	269371.0
48	269371.0
49	269371.0
50	261698.0
51	254025.0
52	254025.0
53	254025.0
54	228856.0
55	203687.0
56	203687.0
57	203687.0
58	175168.5
59	146650.0
60	146650.0
61	146650.0
62	119721.0
63	92792.0
64	92792.0
65	92792.0
66	71759.0
67	50726.0
68	50726.0
69	50726.0
70	37381.5
71	24037.0
72	24037.0
73	24037.0
74	16874.5
75	9712.0
76	9712.0
77	9712.0
78	6649.0
79	3586.0
80	3586.0
81	3586.0
82	2401.5
83	1217.0
84	1217.0
85	1217.0
86	800.0
87	383.0
88	383.0
89	383.0
90	245.0
91	107.0
92	107.0
93	107.0
94	76.0
95	45.0
96	45.0
97	45.0
98	29.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11437100497832411
2	0.04601132384185453
3	0.03508995466620554
4	0.03119668880266401
5	0.035949506869844584
6	0.0207809385703321
7	0.01936520552904427
8	0.02432027117355168
9	0.02816297514276151
10	0.03569669739818604
11	0.020376443415678434
12	0.019618015000702812
13	0.016837110812458856
14	0.022702290554937013
15	0.02244948108327847
16	0.022095547822956516
17	0.02664611831281026
18	0.019415767423375977
19	0.03352253594192259
20	0.023309033286917514
21	0.028011289459766386
22	0.036960744756478744
23	0.029427022501054216
24	0.038780972952420244
25	0.03165174585164938
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1977774.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.08170762407505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.79418809755032	68.63832606599448
2	9.400855884336755	14.868714729223695
3	2.15683604289708	5.116988320123615
4	0.7334686745292675	2.3201582108216554
5	0.31382063963476636	1.2408736034998407
6	0.16386490287433447	0.7775229803373345
7	0.09441917325307876	0.5226780617314802
8	0.06633052595710315	0.4196425008232602
9	0.045482770620098534	0.32371696513003523
>10	0.21127834421975203	2.88157691971684
>50	0.010875639760280153	0.5958669984312623
>100	0.007096413581804153	1.1259300703264907
>500	7.736821488824641E-4	0.415338102780761
>1k	7.092086364754999E-4	0.7526664710592916
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	5.056189433170827E-5	0.0	0.0	0.0	0.0
9	5.056189433170827E-5	0.0	0.0	0.0	0.0
10	5.056189433170827E-5	0.0	0.0	0.0	0.0
11	5.056189433170827E-5	0.0	0.0	0.0	0.0
12	5.056189433170827E-5	0.0	0.0	0.0	0.0
13	5.056189433170827E-5	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	560	0.0	13.403855	4
GCCGGTT	90	5.418897E-7	12.664458	11
GTAGGAC	815	0.0	12.473688	3
ATAGGAC	260	0.0	12.058945	3
GTCCTAC	1000	0.0	11.795246	1
GTGTAGG	425	0.0	11.190935	1
TGTAGGA	1065	0.0	11.151392	2
CTGTAGG	1035	0.0	10.936842	1
TAGGACA	405	0.0	10.791789	4
AGGACCT	880	0.0	10.688339	5
TCCTACA	1165	0.0	10.683513	2
TGCGCCA	125	2.2460881E-7	10.6381445	12
TTGGACT	210	0.0	10.406368	4
TATGGCG	805	0.0	10.384333	16
CCGGTTT	120	1.5274272E-6	10.289872	12
CTACACT	450	0.0	10.134898	4
GGACCTG	825	0.0	10.132335	6
TGGCGAG	780	0.0	10.109001	18
TAGGACG	520	0.0	10.049629	4
CCTACAG	1230	0.0	9.887205	3
>>END_MODULE