FastQCFastQC Report
Thu 26 May 2016
SRR1513045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513045_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977774
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28450.14384858937371003No Hit
GTACATGGGAAGCAGTGGTATCAAC22980.1161912331742656No Hit
CCCATGTACTCTGCGTTGATACCAC21450.10845526334151426No Hit
TATCAACGCAGAGTACTTTTTTTTT20400.10314626443668488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1251.05501385E-1012.90170419
TAGGACC5600.012.4112744
GTACTAG1551.8189894E-1212.2944931
GTAGGAC9250.012.1456793
TCCAACG2050.012.03536918
CTACACG802.8032782E-511.9012214
GTCCTAC10550.011.83126451
TGTAGGA10300.011.5613332
GTGCATT7650.011.16064311
GCCGGTT1201.3009958E-711.06763711
GTCTATA2300.010.7710451
CTGTAGG11000.010.4810551
TAATACT3500.010.3370614
TAGGACG5900.010.1664684
CCGGTTT1502.4760084E-810.12512812
CTAAGAC2950.010.00509553
CTGCTCG1054.16901E-59.9385389
AGGACGT6050.09.9144065
TAAGACT2600.09.8871694
AGTACTG3100.09.8281055
Produced by FastQC (version 0.11.5)