FastQCFastQC Report
Thu 26 May 2016
SRR1513044_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513044_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568094
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC51440.32804155873308616No Hit
CCCATGTACTCTGCGTTGATACCAC40660.2592956799783686No Hit
GTATCAACGCAGAGTACTTTTTTTT22210.14163691717460816No Hit
GAGTACATGGGAAGCAGTGGTATCA21600.13774684425806105No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCGT500.001500582213.2987956
CCAACGA655.4522894E-513.15391319
TAGGACC4050.013.1367074
CCGGTTT604.0962955E-412.66511312
GTAGGAC8150.012.4728543
GGACCGC701.0918357E-412.2131796
GTCCTAC7650.011.9345741
ACCGTGC658.033894E-411.6905028
TCCAACG1157.072049E-811.56527618
TGTAGGA9000.011.5063482
TAGGACT2450.011.2456714
TCCTACA9250.011.1953652
CTAGGAC2050.011.1223623
CGTGGAA3100.011.0309069
AGGACGT5600.011.0271935
GGACGTG5450.010.8063596
GTAACAC1605.4387783E-1010.6878953
TACACTG7600.010.6253935
GACGTGG3150.010.55392557
ACGTGGA3450.010.46215258
Produced by FastQC (version 0.11.5)