FastQCFastQC Report
Thu 26 May 2016
SRR1513044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513044_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568094
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC60330.3847345886152233No Hit
CCCATGTACTCTGCGTTGATACCAC52420.3342911840744241No Hit
GTATCAACGCAGAGTACTTTTTTTT33110.2111480561752038No Hit
TATCAACGCAGAGTACTTTTTTTTT23920.15254187567837132No Hit
GAGTACATGGGAAGCAGTGGTATCA23660.15088381181230207No Hit
GGTATCAACGCAGAGTACTTTTTTT22080.14080788524157353No Hit
GTATCAACGCAGAGTACATGGGAAG15990.10197092776325908No Hit
CATGTACTCTGCGTTGATACCACTG15820.10088680908159843No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT400.00521038714.2781774
AGTGCGT400.00532539314.2312698
TGGGTCG500.001477397413.3262995
GCGCCAC751.4899075E-512.65728413
CCCGTCC604.1377137E-412.6500178
GTAGGAC7550.012.3555093
TGTACCG701.0709762E-412.2384375
AAGTCGT550.0030988412.0750177
AATGCCG1302.6575435E-911.68029118
CTAGACT2150.011.5110884
GTCCTAC8000.011.4335171
CTAGATA1251.7786078E-811.4225424
TGTAGGA9000.011.3247492
TAGGACC4350.011.1599534
CGTCAAG600.00593349911.06876511
GGACGTG5250.011.0599216
TGGCGAG6100.011.04601418
TAGGACG5100.011.0119284
TCTAGAC2100.010.8786123
ATGCCGG1408.51287E-910.84356319
Produced by FastQC (version 0.11.5)