##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513044_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1568094 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.633393788892757 31.0 31.0 34.0 27.0 34.0 2 30.772246434206114 31.0 31.0 34.0 27.0 34.0 3 30.768683510044678 33.0 31.0 34.0 27.0 34.0 4 34.02250439068066 37.0 35.0 37.0 30.0 37.0 5 33.97171598131234 37.0 35.0 37.0 30.0 37.0 6 33.94171395337269 37.0 35.0 37.0 30.0 37.0 7 33.86323523972415 37.0 35.0 37.0 29.0 37.0 8 33.8575397903442 37.0 35.0 37.0 29.0 37.0 9 35.41665550662142 39.0 35.0 39.0 30.0 39.0 10 35.326789720514206 39.0 35.0 39.0 29.0 39.0 11 35.3474071069719 39.0 35.0 39.0 29.0 39.0 12 35.258425196448684 39.0 35.0 39.0 29.0 39.0 13 35.242058192939965 39.0 35.0 39.0 29.0 39.0 14 36.426610904703416 40.0 37.0 41.0 29.0 41.0 15 36.423196568573054 40.0 37.0 41.0 29.0 41.0 16 36.387747163116494 40.0 37.0 41.0 29.0 41.0 17 36.304138654953086 40.0 37.0 41.0 28.0 41.0 18 36.222182471203894 40.0 36.0 41.0 27.0 41.0 19 36.29322476841312 40.0 37.0 41.0 27.0 41.0 20 36.19527528324195 40.0 37.0 41.0 27.0 41.0 21 36.21802264405068 40.0 37.0 41.0 27.0 41.0 22 36.19490030572147 40.0 37.0 41.0 28.0 41.0 23 36.13611620221747 40.0 37.0 41.0 28.0 41.0 24 36.01338695256789 40.0 37.0 41.0 27.0 41.0 25 35.99470057279729 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4809331139891704 1101 2 0.3152728516717289 1101 3 0.49261933567853333 1101 4 0.6756026983032228 1101 5 0.4405773050937256 1101 6 0.40585005018250797 1101 7 0.4306085867510774 1101 8 0.4429739168109492 1101 9 0.384673890550026 1101 10 0.32142255136872677 1101 11 0.4452718584606359 1101 12 0.3264325454538124 1101 13 0.34795029900389807 1101 14 0.44893260314273675 1101 15 0.4093170455794777 1101 16 0.506470743431997 1101 17 0.43528185744106906 1101 18 0.23905683412100842 1101 19 0.22201412291379796 1101 20 0.5204904465232829 1101 21 0.5133682501089609 1101 22 0.5728453493806711 1101 23 0.47880867108752767 1101 24 0.5312448666564507 1101 25 0.678849791535896 1102 1 0.462538171658057 1102 2 0.3776096952851695 1102 3 0.3959254464314519 1102 4 0.7368173268582723 1102 5 0.546364252582805 1102 6 0.42689776133660473 1102 7 0.35546029532618917 1102 8 0.4073366935141536 1102 9 0.4308605801409655 1102 10 0.3032966111151225 1102 11 0.33621139567677005 1102 12 0.3332342504470063 1102 13 0.4077031466290819 1102 14 0.4402833445998624 1102 15 0.4162337301557315 1102 16 0.4836718323352116 1102 17 0.3506536605788355 1102 18 0.35903605899384416 1102 19 0.4547850950464749 1102 20 0.47436214220348916 1102 21 0.5255926214822679 1102 22 0.5042354919410243 1102 23 0.5620298903725711 1102 24 0.6249378246047286 1102 25 0.5456124536177072 1103 1 -0.01166292240164779 1103 2 0.13051579137892588 1103 3 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37696.0 3 0.0 4 638.0 5 4781.0 6 5624.0 7 4222.0 8 3407.0 9 3089.0 10 2709.0 11 2434.0 12 2340.0 13 2156.0 14 2100.0 15 2147.0 16 2262.0 17 2297.0 18 2594.0 19 3178.0 20 4087.0 21 5457.0 22 6636.0 23 8404.0 24 9710.0 25 10577.0 26 11942.0 27 12926.0 28 14834.0 29 17645.0 30 22379.0 31 28498.0 32 36327.0 33 47810.0 34 63850.0 35 86143.0 36 126355.0 37 212983.0 38 757857.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.37220648661842 19.003015144728863 13.449053382565577 27.17572498608714 2 13.54989269667331 19.7238383130628 40.32422643012074 26.402042560143148 3 17.78209724672118 23.432842673972353 29.013758103787147 29.771301975519325 4 10.333627958527996 16.660225726263857 37.481043865992724 35.52510244921542 5 12.660455427116798 37.96446364753735 35.80405191213437 13.571029013211481 6 33.17063677010617 36.77625210626322 17.174687965746653 12.878423157883956 7 28.782141217284835 30.89070710678027 20.979605632365693 19.347546043569206 8 25.417031868570696 37.84614442475437 19.16133057247073 17.575493134204205 9 25.76239009494113 15.383486762911271 19.45160860243314 39.40251453971445 10 14.01597138874044 30.065482438742542 34.145507743993505 21.77303842852351 11 35.186050857153425 22.66333936847234 22.63534261584055 19.515267158533682 12 22.708927799256855 25.28696998725712 31.158263039133026 20.845839174353 13 28.995073841357794 21.203273561247894 25.332088400439805 24.469564196954508 14 20.545869532360545 21.909586771483617 27.624074736602456 29.920468959553386 15 23.908084533251536 29.869210563404085 22.822370867034035 23.400334036310348 16 22.126666789973143 28.7017932800947 27.183018912449636 21.98852101748252 17 22.11200449064078 28.615842904117805 27.222085787823602 22.050066817417818 18 21.748333636453555 28.002756798667548 28.653667595171772 21.59524196970712 19 22.966195544282865 27.45214990520565 27.834325505676176 21.747329044835304 20 23.033376825623975 27.35881905038856 28.097295187660944 21.510508936326524 21 22.562104057537365 26.927531130149085 27.79948140864004 22.710883403673503 22 22.481751731720166 27.630040035865193 27.284014861353974 22.604193371060664 23 22.007105464039352 27.388879075103517 27.517251890677123 23.086763570180008 24 22.211791955288167 27.608753601524405 28.040107494104276 22.139346949083148 25 22.47531079131736 27.01400553793331 27.573793407793158 22.936890262956176 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 69.0 1 69.0 2 172.5 3 276.0 4 276.0 5 276.0 6 784.5 7 1293.0 8 1293.0 9 1293.0 10 1993.5 11 2694.0 12 2694.0 13 2694.0 14 4574.5 15 6455.0 16 6455.0 17 6455.0 18 11457.0 19 16459.0 20 16459.0 21 16459.0 22 26243.5 23 36028.0 24 36028.0 25 36028.0 26 50769.5 27 65511.0 28 65511.0 29 65511.0 30 84643.0 31 103775.0 32 103775.0 33 103775.0 34 120584.5 35 137394.0 36 137394.0 37 137394.0 38 152773.0 39 168152.0 40 168152.0 41 168152.0 42 181196.5 43 194241.0 44 194241.0 45 194241.0 46 210492.0 47 226743.0 48 226743.0 49 226743.0 50 218038.0 51 209333.0 52 209333.0 53 209333.0 54 183585.0 55 157837.0 56 157837.0 57 157837.0 58 133908.5 59 109980.0 60 109980.0 61 109980.0 62 89059.0 63 68138.0 64 68138.0 65 68138.0 66 52407.0 67 36676.0 68 36676.0 69 36676.0 70 26723.5 71 16771.0 72 16771.0 73 16771.0 74 11686.0 75 6601.0 76 6601.0 77 6601.0 78 4540.5 79 2480.0 80 2480.0 81 2480.0 82 1660.5 83 841.0 84 841.0 85 841.0 86 554.5 87 268.0 88 268.0 89 268.0 90 167.5 91 67.0 92 67.0 93 67.0 94 39.0 95 11.0 96 11.0 97 11.0 98 6.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1909962030337467 2 0.06638632632992665 3 0.0 4 0.0 5 0.01855756096254434 6 0.38811448803451837 7 8.928036201911366E-4 8 0.029845149589246563 9 0.03131189839384629 10 0.00255086748626039 11 0.003507442793608036 12 0.01020346994504156 13 0.008800492827598346 14 0.0017218355532257633 15 6.377168715650975E-5 16 0.011797762123954305 17 0.02480718630388229 18 0.06753421669874382 19 0.031248126706689777 20 0.0 21 0.0 22 0.0 23 3.1885843578254876E-4 24 5.10173497252078E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1568094.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.43414158223591 #Duplication Level Percentage of deduplicated Percentage of total 1 85.64988261774134 64.60925371888585 2 10.273782802637193 15.499879730385487 3 2.2836179483487857 5.167882789064321 4 0.7387365987155923 2.229038447179655 5 0.34092173001628656 1.2858569025254682 6 0.1743399319064857 0.7890709864122726 7 0.11379487711796701 0.6008813210294903 8 0.078041801033637 0.4709613014803246 9 0.05201922992866803 0.3531623359894225 >10 0.2670422166247461 3.5047104108094724 >50 0.014789495522216036 0.7601775711682655 >100 0.010069436884728968 1.576344507553196 >500 0.001269134366719956 0.6139872744587009 >1k 0.001522961240063947 1.8191766407376668 >5k 1.692179155626608E-4 0.7196160623203589 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 6033 0.3847345886152233 No Hit CCCATGTACTCTGCGTTGATACCAC 5242 0.3342911840744241 No Hit GTATCAACGCAGAGTACTTTTTTTT 3311 0.2111480561752038 No Hit TATCAACGCAGAGTACTTTTTTTTT 2392 0.15254187567837132 No Hit GAGTACATGGGAAGCAGTGGTATCA 2366 0.15088381181230207 No Hit GGTATCAACGCAGAGTACTTTTTTT 2208 0.14080788524157353 No Hit GTATCAACGCAGAGTACATGGGAAG 1599 0.10197092776325908 No Hit CATGTACTCTGCGTTGATACCACTG 1582 0.10088680908159843 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 6.377168715650975E-5 0.0 8 0.0 0.0 0.0 6.377168715650975E-5 0.0 9 0.0 0.0 0.0 6.377168715650975E-5 0.0 10 0.0 0.0 0.0 6.377168715650975E-5 0.0 11 0.0 0.0 0.0 1.275433743130195E-4 0.0 12 0.0 0.0 0.0 1.275433743130195E-4 6.377168715650975E-5 13 0.0 0.0 0.0 1.275433743130195E-4 6.377168715650975E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACGCT 40 0.005210387 14.278177 4 AGTGCGT 40 0.005325393 14.231269 8 TGGGTCG 50 0.0014773974 13.326299 5 GCGCCAC 75 1.4899075E-5 12.657284 13 CCCGTCC 60 4.1377137E-4 12.650017 8 GTAGGAC 755 0.0 12.355509 3 TGTACCG 70 1.0709762E-4 12.238437 5 AAGTCGT 55 0.00309884 12.075017 7 AATGCCG 130 2.6575435E-9 11.680291 18 CTAGACT 215 0.0 11.511088 4 GTCCTAC 800 0.0 11.433517 1 CTAGATA 125 1.7786078E-8 11.422542 4 TGTAGGA 900 0.0 11.324749 2 TAGGACC 435 0.0 11.159953 4 CGTCAAG 60 0.005933499 11.068765 11 GGACGTG 525 0.0 11.059921 6 TGGCGAG 610 0.0 11.046014 18 TAGGACG 510 0.0 11.011928 4 TCTAGAC 210 0.0 10.878612 3 ATGCCGG 140 8.51287E-9 10.843563 19 >>END_MODULE