FastQCFastQC Report
Thu 26 May 2016
SRR1513043_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513043_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760925
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC35900.20387012507630933No Hit
GTATCAACGCAGAGTACTTTTTTTT33300.18910515780058776No Hit
CCCATGTACTCTGCGTTGATACCAC26920.15287419963939408No Hit
TATCAACGCAGAGTACTTTTTTTTT19270.10943112284736715No Hit
GTCCTACAGTGGACATTTCTAAATT18190.10329798259437512No Hit
GGTATCAACGCAGAGTACTTTTTTT18170.10318440592302341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4550.012.1116644
CCAACGA550.003071232212.0895919
AGGCGTG951.0421081E-611.9980097
GTCCTAC10800.011.9793551
AGGACGT8200.011.8177985
ACGTGGA4300.011.707368
TACACCG658.020222E-411.6930255
GACGTGG4400.011.4412837
GGACGTG7900.011.4228046
GTATTAC1751.8189894E-1211.4156211
GACAGCG752.0773118E-411.39810857
GTAGGAC10100.011.1947113
GTCTTAC1701.0913936E-1111.1917851
AGGACCT7650.011.17715555
TCCTACA12500.011.0979412
TTAGGAC6600.010.9409983
GGACCTG7150.010.8939166
CGTGGAA4700.010.7112929
CGTGCGC803.7760058E-410.68602910
GTGCGCC1252.2461063E-710.63793211
Produced by FastQC (version 0.11.5)