##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513043_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1760925
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.325372176554936	31.0	30.0	34.0	25.0	34.0
2	29.000319150446497	31.0	30.0	34.0	16.0	34.0
3	29.380237091301446	31.0	30.0	34.0	23.0	34.0
4	32.27145676278092	35.0	33.0	37.0	19.0	37.0
5	32.53080057356219	35.0	33.0	37.0	23.0	37.0
6	32.96638357681332	37.0	35.0	37.0	27.0	37.0
7	32.962382838564956	37.0	35.0	37.0	26.0	37.0
8	32.9431151241535	37.0	35.0	37.0	26.0	37.0
9	34.314642020528986	39.0	35.0	39.0	26.0	39.0
10	33.83521558271931	39.0	34.0	39.0	22.0	39.0
11	34.19499297244346	39.0	35.0	39.0	26.0	39.0
12	34.030617431179635	39.0	35.0	39.0	25.0	39.0
13	34.110836066271986	39.0	35.0	39.0	25.0	39.0
14	34.42740207561367	40.0	34.0	41.0	17.0	41.0
15	34.97268197112313	40.0	34.0	41.0	24.0	41.0
16	34.57449749421469	39.0	34.0	41.0	17.0	41.0
17	34.12466629754248	39.0	33.0	41.0	16.0	41.0
18	33.55525533455428	39.0	32.0	41.0	10.0	41.0
19	34.45605860556242	39.0	34.0	41.0	18.0	41.0
20	34.709828073313744	40.0	34.0	41.0	18.0	41.0
21	34.73279895509462	40.0	35.0	41.0	18.0	41.0
22	34.32753126907733	40.0	34.0	41.0	11.0	41.0
23	32.68098584550733	39.0	31.0	41.0	8.0	41.0
24	33.77326348368045	39.0	33.0	41.0	2.0	41.0
25	33.515646038303736	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9376481245816137
1101	2	1.277728546636638
1101	3	1.1002656749973987
1101	4	1.2294020987469736
1101	5	1.3568390409140676
1101	6	0.9756687947878149
1101	7	0.9002685602765723
1101	8	1.019539462420063
1101	9	1.1363468625774331
1101	10	1.4066969797666857
1101	11	0.9884987829657135
1101	12	1.0533845677711824
1101	13	1.0446052189796973
1101	14	0.1844995052514733
1101	15	0.6080390479879583
1101	16	1.2253164890293462
1101	17	1.9490764580190643
1101	18	-4.063170534134613
1101	19	-0.4159517385420486
1101	20	0.469577147719356
1101	21	0.8201955254431468
1101	22	1.4253435254375688
1101	23	3.0250735357720515
1101	24	2.077387373956256
1101	25	2.2161741513526536
1102	1	1.0613824447245221
1102	2	1.2932969365767804
1102	3	1.0270556118991863
1102	4	1.648150497581355
1102	5	1.4081093037914911
1102	6	1.1521700158663535
1102	7	1.137574750563573
1102	8	0.9904617164680118
1102	9	0.9893351364066802
1102	10	1.5403265757264606
1102	11	1.1389097512176747
1102	12	1.1526785224059353
1102	13	1.0127366015945185
1102	14	1.989605481452152
1102	15	1.4271271397108265
1102	16	1.6656845995353606
1102	17	1.2679150136823765
1102	18	1.6304836164047387
1102	19	1.488805291984626
1102	20	1.3708802869003307
1102	21	1.363183099530488
1102	22	1.8545013079843926
1102	23	3.2756479313482956
1102	24	2.1810347641791807
1102	25	2.5169133910859642
1103	1	0.7644805217801434
1103	2	1.0286577471081735
1103	3	0.7570292489520476
1103	4	1.1409989020199731
1103	5	1.0013115116165139
1103	6	0.6891864079907819
1103	7	0.621581229300908
1103	8	0.6355733713029466
1103	9	0.7970309344171085
1103	10	0.954028128785481
1103	11	0.8386435371436534
1103	12	0.9733389745390895
1103	13	0.7334487457808905
1103	14	1.3509685434596364
1103	15	1.0010215041283104
1103	16	1.1132056784059472
1103	17	1.7175833230076236
1103	18	1.4890423845256748
1103	19	1.169878821058532
1103	20	1.0999625232134278
1103	21	1.0702718030937959
1103	22	1.2539450440562518
1103	23	2.650269430647583
1103	24	1.6738265945373527
1103	25	1.7528290296551319
1104	1	0.6531031942883594
1104	2	1.05766924765582
1104	3	0.8915788108359486
1104	4	1.3330443565654306
1104	5	1.154150138852735
1104	6	0.7430997887866937
1104	7	0.6424670452921504
1104	8	0.5969575443588013
1104	9	0.9034361005361333
1104	10	1.130141673874931
1104	11	0.6715433180855968
1104	12	0.8183006393802756
1104	13	0.686727770972567
1104	14	1.4971304638685439
1104	15	0.9895255201925721
1104	16	1.1363550942511438
1104	17	1.7785019977064138
1104	18	2.34033163242637
1104	19	1.499463067133739
1104	20	0.836992461146977
1104	21	0.9567175862263255
1104	22	1.3514076216393107
1104	23	2.7218101389352753
1104	24	1.7272448128652087
1104	25	1.798673772261921
1105	1	0.6940149806223808
1105	2	1.036928207574391
1105	3	0.6726435704325766
1105	4	0.9413900953075256
1105	5	1.0044318948308728
1105	6	0.7134726740755255
1105	7	0.4563462595813874
1105	8	0.4933727081774535
1105	9	0.6340185064530601
1105	10	1.2019255529715167
1105	11	0.7882514980327784
1105	12	0.7517920482610521
1105	13	0.7146947281201008
1105	14	1.4508867805382764
1105	15	0.9036762616025698
1105	16	1.156441347339424
1105	17	1.6551670595167423
1105	18	1.538589728076552
1105	19	1.2620973874443848
1105	20	0.9159264516680494
1105	21	0.7893079028332792
1105	22	1.235044980901442
1105	23	2.6842890086381317
1105	24	1.7661764045090464
1105	25	1.7485931464464173
1106	1	-0.43318309393797705
1106	2	-0.04557320740287096
1106	3	-0.2381749696151516
1106	4	-2.5512289212544026
1106	5	-0.5036591832198098
1106	6	-0.5410750445359938
1106	7	-0.43942971282282883
1106	8	-0.3762341436650587
1106	9	-0.2829457820281931
1106	10	-0.1438731971154681
1106	11	-0.15155414367666253
1106	12	-0.2488781818851038
1106	13	-0.22141929495959545
1106	14	0.4430486939425151
1106	15	-0.10384854381516817
1106	16	-0.86500756683985
1106	17	-5.741074890467019
1106	18	-7.096545037766898
1106	19	-2.8249463228865963
1106	20	-1.3874262203922
1106	21	-0.8981990523187733
1106	22	-1.2499411876782034
1106	23	-5.0070918429513895
1106	24	-2.0142942046735293
1106	25	-0.6845875280490858
1107	1	0.5764459086908253
1107	2	0.8707338693812332
1107	3	0.5853272729557624
1107	4	0.8626760122911747
1107	5	0.8602606860744473
1107	6	0.4941139658450382
1107	7	0.5337853271724029
1107	8	0.5750452233021477
1107	9	0.5358264386940164
1107	10	1.0184262124669345
1107	11	0.7379083405386524
1107	12	0.785525666403025
1107	13	0.7809215592547929
1107	14	1.4253900009226683
1107	15	0.9396064785995009
1107	16	-5.547045631188908
1107	17	-0.7581734238924511
1107	18	0.8175542537651168
1107	19	0.7771984616659182
1107	20	0.7594322201831218
1107	21	0.710424975960315
1107	22	0.7701235136537576
1107	23	2.614101404654612
1107	24	1.6115462417755992
1107	25	1.2242433920979394
1108	1	0.40306967743161337
1108	2	0.5530238113110322
1108	3	0.40345830209475153
1108	4	0.6846764040431736
1108	5	0.6757506357959997
1108	6	0.21929723435532367
1108	7	0.10105249987010012
1108	8	0.2711547039169133
1108	9	0.29207634400258087
1108	10	0.7435872961265488
1108	11	0.337952277364856
1108	12	0.36401539752263545
1108	13	0.37205476142696625
1108	14	0.9291292304482184
1108	15	0.6553736307356317
1108	16	0.8219211716169923
1108	17	1.0348310974883361
1108	18	0.8698231146758388
1108	19	0.7547509098994638
1108	20	0.5884381549012261
1108	21	0.6468296665952806
1108	22	1.025648353111194
1108	23	2.737080418868068
1108	24	1.4418443087716852
1108	25	1.5275592697414524
1109	1	0.2662003781511615
1109	2	0.5616672440711596
1109	3	0.382954196097927
1109	4	0.40350393574593824
1109	5	0.17736397982015006
1109	6	0.2939848072659288
1109	7	0.16455414785522038
1109	8	0.08804912552288613
1109	9	0.20740460467450106
1109	10	0.7240141182482134
1109	11	0.4128301709233071
1109	12	0.4220759287993019
1109	13	0.28318405569061866
1109	14	-0.9279116203042435
1109	15	-0.05556764315851126
1109	16	-0.33000550317732547
1109	17	0.687964459958522
1109	18	1.4649715264431862
1109	19	0.5793225104440793
1109	20	0.46504314672428393
1109	21	0.49343533207127876
1109	22	0.30018862605565033
1109	23	-3.9949216831481813
1109	24	-1.3734317027209428
1109	25	-0.14734882866225973
1110	1	0.5642780661897575
1110	2	0.8549835046839327
1110	3	0.6608628435184016
1110	4	1.082685161163134
1110	5	0.809664437426342
1110	6	0.4358622178131455
1110	7	0.295882430972803
1110	8	0.2745558686906122
1110	9	0.399584487186182
1110	10	0.7542501768523593
1110	11	0.38197556431188673
1110	12	0.505653499094997
1110	13	0.31217784713930286
1110	14	1.1379134232924528
1110	15	0.6174726535535981
1110	16	0.9739684788239202
1110	17	1.3250545873754334
1110	18	1.8616063866155343
1110	19	1.0701596465548775
1110	20	0.7044753236313994
1110	21	0.6548982398754006
1110	22	0.7944426929590733
1110	23	2.1571333282442353
1110	24	1.228750836961595
1110	25	1.5473235437617134
1111	1	0.4445231354165067
1111	2	0.7557884289263548
1111	3	0.5865747034975044
1111	4	0.9843227656563371
1111	5	0.7702509055559119
1111	6	0.2797877337483641
1111	7	0.20687952475545757
1111	8	0.029678484939303473
1111	9	0.10695044199286485
1111	10	0.28653286363396546
1111	11	0.04887080987091963
1111	12	0.12724522453908804
1111	13	0.13685783668997686
1111	14	-0.6496469868433934
1111	15	-0.07918682920502107
1111	16	0.4027416632544316
1111	17	0.882285027553074
1111	18	1.6322478769499185
1111	19	0.6464886695433805
1111	20	0.3233526747285751
1111	21	0.40670646218470097
1111	22	0.7150482637532249
1111	23	0.7635980291324316
1111	24	0.47058322332522806
1111	25	0.14572675692785708
1112	1	0.6301962407354509
1112	2	0.8734472224957273
1112	3	0.6524202810242841
1112	4	1.1758264854369145
1112	5	0.9754294712052882
1112	6	0.40362606083823493
1112	7	0.3233854948111201
1112	8	0.3831647173011987
1112	9	0.5263929957567441
1112	10	0.7009583153071617
1112	11	0.40302871142113617
1112	12	0.43568929331963346
1112	13	0.4476405481045873
1112	14	0.7658142234708549
1112	15	0.5027540305315412
1112	16	1.0652780216317268
1112	17	1.1390900869805662
1112	18	1.310578005958945
1112	19	0.7550626727473642
1112	20	0.6179227862208592
1112	21	0.711905836557662
1112	22	0.7562243788783078
1112	23	2.168159437875431
1112	24	1.2289065863385957
1112	25	1.3127047537886014
1113	1	0.21649005747500283
1113	2	0.6841039728289502
1113	3	0.4493490947169825
1113	4	0.4901633287583209
1113	5	0.6247455617269111
1113	6	0.16129090064701757
1113	7	0.18522559268128447
1113	8	0.13957084338383652
1113	9	0.3669014985590593
1113	10	0.4530221609764524
1113	11	0.13914392113136387
1113	12	0.15713989290970432
1113	13	0.08930335843328407
1113	14	0.8904664795256849
1113	15	0.2809581461557684
1113	16	0.7458494338057733
1113	17	1.1437052871047726
1113	18	1.7114084688109799
1113	19	0.640675355569428
1113	20	0.35306122405499707
1113	21	0.48531932157320057
1113	22	0.6976704448434248
1113	23	0.5016517864121965
1113	24	0.4457298936317784
1113	25	1.0084496497952884
1114	1	-0.5072326318942686
1114	2	-3.301965503732994
1114	3	-1.1756438588118598
1114	4	-0.005895456647991182
1114	5	0.1327060956442736
1114	6	-0.023549695695685102
1114	7	-0.12191897509517702
1114	8	-0.19875023487296062
1114	9	-0.10143321241265824
1114	10	-0.007833002415843282
1114	11	-0.12463395413021061
1114	12	-0.0021936344983615186
1114	13	-0.13685604023213216
1114	14	0.6483077256656458
1114	15	0.08529453537470744
1114	16	0.31879407709362084
1114	17	0.6280770967883171
1114	18	0.8421011195759363
1114	19	0.16549701440674625
1114	20	-0.010370616205669592
1114	21	-0.14742103675401097
1114	22	0.042924033186572785
1114	23	1.6634362073736781
1114	24	0.6432484156540923
1114	25	0.4919693082627248
1115	1	0.10970383721513599
1115	2	0.31405301077247216
1115	3	0.10847366063459418
1115	4	0.35331578025952126
1115	5	0.23939372501027378
1115	6	-0.22825451713620737
1115	7	-0.30265447013110247
1115	8	-0.2834239594646135
1115	9	-0.246714733253107
1115	10	0.06765349994328318
1115	11	-0.03768913922743877
1115	12	-0.22642940747152096
1115	13	-0.4207052472046158
1115	14	0.482360475756316
1115	15	-0.12483487461300768
1115	16	0.3272744990811205
1115	17	0.8106227562612887
1115	18	1.2030660713771155
1115	19	0.4020739802296234
1115	20	0.0306852075508246
1115	21	0.022252001113599817
1115	22	0.2603831447628764
1115	23	2.0370375902221625
1115	24	0.7353870609288222
1115	25	0.4726713587156226
1116	1	0.12523680423701578
1116	2	0.1425806265924443
1116	3	0.08240753940748746
1116	4	0.8085039757381196
1116	5	0.23082140039265653
1116	6	0.08833158596885937
1116	7	-0.10420818155088085
1116	8	0.050978074938115014
1116	9	0.06407216086411438
1116	10	0.5400338690509088
1116	11	0.014033765759421613
1116	12	0.2087826264223338
1116	13	-0.05104134554603945
1116	14	0.7298190975892496
1116	15	0.02546886678972271
1116	16	0.35148638434916535
1116	17	0.7588423512413698
1116	18	1.3735598341366853
1116	19	0.3909588763720109
1116	20	0.22364758459546863
1116	21	0.13786865141946691
1116	22	0.4626492064948238
1116	23	1.7661780241333247
1116	24	0.6623112440528587
1116	25	0.898307451430334
1201	1	0.9633201658213224
1201	2	1.2417357439673609
1201	3	1.0506058498942927
1201	4	1.5445126866997896
1201	5	1.3390543430013864
1201	6	0.965439098039063
1201	7	0.9689289871093223
1201	8	0.9948518567330566
1201	9	1.222452617660288
1201	10	1.5114054044209126
1201	11	1.0846280948124658
1201	12	1.1298496184057782
1201	13	0.9829012846048286
1201	14	1.9790584637760489
1201	15	1.2609703205070701
1201	16	1.7443724323174266
1201	17	2.2058990619711523
1201	18	2.7697082078258504
1201	19	1.9415642453591175
1201	20	1.6686452720065361
1201	21	1.5580837032857318
1201	22	1.8797338825370318
1201	23	3.3209406340443124
1201	24	2.230742199725512
1201	25	2.405978567257641
1202	1	0.8527598669560419
1202	2	1.0814442326532792
1202	3	0.954325487196563
1202	4	1.58007500790643
1202	5	1.2622266979627383
1202	6	0.9659148998054832
1202	7	0.8261266838463612
1202	8	0.7562369031733738
1202	9	1.0106237592168341
1202	10	1.3769513262776414
1202	11	0.9265876332773999
1202	12	1.041716717485798
1202	13	0.9462535176373876
1202	14	1.8976595918748274
1202	15	1.2707533048945976
1202	16	1.5222443408928896
1202	17	1.9013476908237124
1202	18	2.522693551366494
1202	19	1.7288521752905055
1202	20	1.4969801129034295
1202	21	1.3643331057757848
1202	22	1.771957060775165
1202	23	3.4599350523821712
1202	24	2.1214232507546953
1202	25	2.2715333138766596
1203	1	0.9221531383568689
1203	2	1.2427784057493803
1203	3	1.0066400833231555
1203	4	1.5626866701118516
1203	5	1.2949931914283326
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2211	16	-0.686333754592134
2211	17	-0.038158710647948624
2211	18	-5.296969316805576
2211	19	-1.809567766188806
2211	20	-1.11149509811262
2211	21	-0.9852419813673592
2211	22	-0.6225168086274024
2211	23	-4.831026341249693
2211	24	-2.1885647734931126
2211	25	-1.5479488891678983
2212	1	0.31354686084648264
2212	2	0.4546118830623094
2212	3	0.2578421072649064
2212	4	0.8464227349438076
2212	5	0.6263749841799893
2212	6	0.20840221470659515
2212	7	0.12198253970222339
2212	8	0.02054880127837322
2212	9	-0.2376168952307225
2212	10	-3.729479242221892
2212	11	-1.0218621958620844
2212	12	-0.28777691407679384
2212	13	-0.08777361294146857
2212	14	0.4704689529600472
2212	15	0.24480528791209366
2212	16	0.4104456456459147
2212	17	0.983957196881498
2212	18	1.541931409914696
2212	19	0.7790168226965335
2212	20	0.4039367246876253
2212	21	0.3463979292199184
2212	22	0.6061788687900744
2212	23	1.807198177576332
2212	24	1.0199445306552875
2212	25	0.8864572032833067
2213	1	-1.706983317894558
2213	2	-3.92500185207858
2213	3	-1.896916357269916
2213	4	-3.328519363704359
2213	5	-1.580595820490494
2213	6	-1.305681197779375
2213	7	-1.1211931599591018
2213	8	-0.9901979198836841
2213	9	-0.8843378654021663
2213	10	-0.9811846217227966
2213	11	-1.0807924360063836
2213	12	-0.9459541967908223
2213	13	-1.1766970411620221
2213	14	-6.0368892678806
2213	15	-2.8683075693541156
2213	16	-2.1009889838717797
2213	17	-0.9373998150825429
2213	18	-0.18287608128257204
2213	19	-0.8597735331545238
2213	20	-0.9315510954008346
2213	21	-1.2692560634308805
2213	22	-1.124885997993637
2213	23	-5.890462166473792
2213	24	-3.2721588187004578
2213	25	-2.3539964132725473
2214	1	-0.11295737924266191
2214	2	0.1299441965078323
2214	3	-0.1722438277266889
2214	4	0.18882702602734724
2214	5	-0.014312495752776044
2214	6	-0.20526994802217757
2214	7	-0.2785553566847341
2214	8	-0.4097429999568263
2214	9	-0.46541903699512943
2214	10	0.11240037324262886
2214	11	-0.355399884494112
2214	12	-0.43226819781336445
2214	13	-0.4508668949026742
2214	14	0.06711427752990318
2214	15	-0.27200435191641503
2214	16	0.13285182541058305
2214	17	0.3859882845729601
2214	18	1.0196019106040666
2214	19	0.08273299169572113
2214	20	-0.05363556458277685
2214	21	-0.28217403897468785
2214	22	0.272573943109677
2214	23	1.5869983551123568
2214	24	0.594622604956001
2214	25	0.46329215143900626
2215	1	-2.63800661372586
2215	2	-4.679832818929562
2215	3	-2.7823575435007832
2215	4	-4.156106586830795
2215	5	-4.8891616158849125
2215	6	-2.408833889321965
2215	7	-2.0205285477145267
2215	8	-2.3048640214929392
2215	9	-2.1319713526040474
2215	10	-3.537088223415779
2215	11	-2.3126643530793345
2215	12	-1.977068401104134
2215	13	-2.0100118031041916
2215	14	-1.3739313993648352
2215	15	-2.0374576268036506
2215	16	-2.716954413074898
2215	17	-7.076606213870594
2215	18	-3.395956186086103
2215	19	-2.472867033715623
2215	20	-2.290138963350323
2215	21	-2.3200369447621227
2215	22	-2.0257362023702754
2215	23	-6.418998722582835
2215	24	-3.9527886141533273
2215	25	-3.528474572403187
2216	1	-1.1582346135518158
2216	2	-0.6853063262016938
2216	3	-0.8730305277198767
2216	4	-3.3874162575867715
2216	5	-1.3990885167040723
2216	6	-1.141733100849283
2216	7	-1.1894941439239979
2216	8	-1.0846505636406185
2216	9	-1.018433118663765
2216	10	-0.8168810632685748
2216	11	-1.252546231032099
2216	12	-2.0408117112237463
2216	13	-1.3691956892233605
2216	14	-6.841927034482669
2216	15	-3.2809767157203744
2216	16	-1.7633671257369485
2216	17	-0.9906776916786271
2216	18	-0.25051389253060563
2216	19	-0.7361256495487396
2216	20	-1.2452406770131432
2216	21	-1.4022248591224908
2216	22	-0.8200207487179512
2216	23	0.26234209318753443
2216	24	-0.499970791876855
2216	25	-0.3387961675775415
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	82343.0
3	1.0
4	713.0
5	4130.0
6	4983.0
7	4506.0
8	4226.0
9	4020.0
10	3687.0
11	3662.0
12	3671.0
13	3545.0
14	3520.0
15	3705.0
16	3705.0
17	4112.0
18	4545.0
19	5388.0
20	6692.0
21	8222.0
22	10181.0
23	12491.0
24	14900.0
25	17449.0
26	20107.0
27	22777.0
28	25915.0
29	30661.0
30	36741.0
31	41321.0
32	53636.0
33	70561.0
34	91455.0
35	122258.0
36	171928.0
37	244301.0
38	614867.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.88500088979555	18.653412408582746	12.485565696761087	24.976021004860616
2	13.69586177156879	20.11486840811976	42.6865844630507	23.50268535726075
3	18.92435099646995	25.065073617547778	29.35152478478272	26.659050601199556
4	10.305823113855999	16.682742887266496	40.32313771469991	32.68829628417759
5	11.929649154699145	38.44869114706417	36.68531857844029	12.936341119796403
6	31.17686417197832	37.267024651907256	18.88561474138842	12.670496434726008
7	28.059654697066165	31.587828915045392	22.3105886121712	18.04192777571724
8	25.00984075863303	36.9237760112693	20.764199518896245	17.302183711201423
9	26.69134984773719	15.207138203281787	20.150212190908764	37.95129975807226
10	13.493050415236766	29.75366424646414	35.775009217068934	20.978276121230163
11	34.20571481151414	22.87713785717572	24.260770437974237	18.656376893335903
12	22.64022128694032	25.38091911326188	32.32885193200084	19.650007667796956
13	28.634830120522984	22.074562947985417	25.90659184623948	23.384015085252123
14	20.278875433027128	22.11947146692749	29.253747789779567	28.34790531026582
15	23.3289256799203	30.40610619036746	23.75276472332319	22.51220340638905
16	21.594715332861895	28.638931252183248	28.379809890574904	21.38654352437996
17	21.199096153540292	28.507006059724755	28.661225713954636	21.632672072780316
18	20.61725618227443	27.81736659930319	30.671908067599755	20.893469150822618
19	22.606389324086816	27.493419012096293	28.617895969833683	21.28229569398321
20	22.865786294342143	27.501060752279265	28.88762160310768	20.74553135027091
21	22.817954758081328	27.05602611155008	28.014135046383103	22.11188408398549
22	22.318070293340543	27.992983050134946	28.138582487506454	21.550364169018053
23	20.843510643857375	27.38864791542172	29.964236457455247	21.80360498326566
24	21.92813671169981	27.617129070210687	28.807919743628634	21.64681447446086
25	22.07593685561886	27.283132906538814	28.71745786492919	21.92347237291313
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	162.0
1	162.0
2	304.5
3	447.0
4	447.0
5	447.0
6	1025.5
7	1604.0
8	1604.0
9	1604.0
10	2597.0
11	3590.0
12	3590.0
13	3590.0
14	6088.5
15	8587.0
16	8587.0
17	8587.0
18	15457.0
19	22327.0
20	22327.0
21	22327.0
22	35133.5
23	47940.0
24	47940.0
25	47940.0
26	67596.5
27	87253.0
28	87253.0
29	87253.0
30	111171.0
31	135089.0
32	135089.0
33	135089.0
34	155768.5
35	176448.0
36	176448.0
37	176448.0
38	191830.0
39	207212.0
40	207212.0
41	207212.0
42	218044.5
43	228877.0
44	228877.0
45	228877.0
46	236226.5
47	243576.0
48	243576.0
49	243576.0
50	229469.5
51	215363.0
52	215363.0
53	215363.0
54	186867.5
55	158372.0
56	158372.0
57	158372.0
58	132365.0
59	106358.0
60	106358.0
61	106358.0
62	84417.5
63	62477.0
64	62477.0
65	62477.0
66	47257.5
67	32038.0
68	32038.0
69	32038.0
70	23255.0
71	14472.0
72	14472.0
73	14472.0
74	9984.0
75	5496.0
76	5496.0
77	5496.0
78	3825.0
79	2154.0
80	2154.0
81	2154.0
82	1430.0
83	706.0
84	706.0
85	706.0
86	490.5
87	275.0
88	275.0
89	275.0
90	171.5
91	68.0
92	68.0
93	68.0
94	44.0
95	20.0
96	20.0
97	20.0
98	17.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11891477490523447
2	0.04679358859690219
3	0.03469767309794568
4	0.03185825631415307
5	0.03435694308389057
6	0.01879693910870707
7	0.01851299743032781
8	0.022147450913582348
9	0.02737197779576075
10	0.03430015474821472
11	0.019421610801141447
12	0.017888325737893437
13	0.015048908954100828
14	0.02010307082925167
15	0.019932705822224114
16	0.020443800843306784
17	0.025497962718457633
18	0.01856978576600366
19	0.031290372957394556
20	0.02169314422817553
21	0.02714482445305734
22	0.03498161477632494
23	0.02686088277467808
24	0.037821031560117555
25	0.029245992873063873
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1760925.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.18339279964978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.79017688878947	72.89471199912632
2	7.5756845306221	12.300395459514487
3	1.447658536198562	3.525774947519217
4	0.44688577700108323	1.4511881428342255
5	0.2153714310929669	0.8742291744121519
6	0.12512921165039062	0.6095048364074505
7	0.07943337006264135	0.45140693382367486
8	0.05286586655755383	0.3433464328348609
9	0.03908130524436853	0.2855477659298925
>10	0.20393783843020474	2.9010333203657184
>50	0.011418201477776233	0.649374359704262
>100	0.01030977722858364	1.6569130777628294
>500	0.0011295258727473455	0.6845311135434506
>1k	9.177397716072173E-4	1.3720424362215502
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3590	0.20387012507630933	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3330	0.18910515780058776	No Hit
CCCATGTACTCTGCGTTGATACCAC	2692	0.15287419963939408	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1927	0.10943112284736715	No Hit
GTCCTACAGTGGACATTTCTAAATT	1819	0.10329798259437512	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1817	0.10318440592302341	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.678833567585218E-5	0.0
8	0.0	0.0	0.0	1.7036500702755653E-4	0.0
9	0.0	0.0	0.0	3.4073001405511306E-4	0.0
10	0.0	0.0	0.0	3.9751834973096524E-4	0.0
11	0.0	0.0	0.0	3.9751834973096524E-4	0.0
12	0.0	0.0	0.0	3.9751834973096524E-4	0.0
13	5.678833567585218E-5	0.0	0.0	3.9751834973096524E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	455	0.0	12.111664	4
CCAACGA	55	0.0030712322	12.08959	19
AGGCGTG	95	1.0421081E-6	11.998009	7
GTCCTAC	1080	0.0	11.979355	1
AGGACGT	820	0.0	11.817798	5
ACGTGGA	430	0.0	11.70736	8
TACACCG	65	8.020222E-4	11.693025	5
GACGTGG	440	0.0	11.441283	7
GGACGTG	790	0.0	11.422804	6
GTATTAC	175	1.8189894E-12	11.415621	1
GACAGCG	75	2.0773118E-4	11.3981085	7
GTAGGAC	1010	0.0	11.194711	3
GTCTTAC	170	1.0913936E-11	11.191785	1
AGGACCT	765	0.0	11.1771555	5
TCCTACA	1250	0.0	11.097941	2
TTAGGAC	660	0.0	10.940998	3
GGACCTG	715	0.0	10.893916	6
CGTGGAA	470	0.0	10.711292	9
CGTGCGC	80	3.7760058E-4	10.686029	10
GTGCGCC	125	2.2461063E-7	10.637932	11
>>END_MODULE