FastQCFastQC Report
Thu 26 May 2016
SRR1513043_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513043_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760925
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51000.2896205119468461No Hit
GTACATGGGAAGCAGTGGTATCAAC40620.23067421951531156No Hit
TATCAACGCAGAGTACTTTTTTTTT34130.19381858966168347No Hit
CCCATGTACTCTGCGTTGATACCAC34100.19364822465465595No Hit
GGTATCAACGCAGAGTACTTTTTTT29570.1679231085934949No Hit
GTCCTACAGTGGACATTTCTAAATT19120.10857929781222936No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17810.10114002583869272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCGC350.00219225916.2612468
GGGCGAA500.001516446913.28039511
TACACCG550.003026407412.1133435
TAGGACC4800.011.69875054
GCCCTAC855.1576004E-511.2112671
GCCCCGG600.005741755511.1178411
AAGTCGT600.005939794211.067317
GGCGAGG3500.010.84144619
GCGCCAC1502.1518645E-910.76088213
TTACACC1553.5524863E-910.4386874
GTATTAG1105.8253336E-610.3959021
ACTGACC2300.010.3105728
TAGCACT2500.010.2790384
GTAGGAC11200.010.1974573
GCACCGC750.002607454310.1521366
TGGCGAG9000.010.01868718
CTAGTAC951.6136291E-410.0185553
GTAATAC1356.280552E-79.87013153
AGGACGT8600.09.8496375
GTCCTAC10450.09.8487491
Produced by FastQC (version 0.11.5)