FastQCFastQC Report
Thu 26 May 2016
SRR1513042_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513042_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2509389
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAG500.001501490413.29824210
GGTATCA9800.013.1995591
ATACCGT751.4811489E-512.6652446
CGTGTAA701.09276305E-412.21267110
CGAAATC658.031312E-411.69146213
GCGCTAG600.005824918411.0966871
CTACGAC600.005878787511.0829733
TCGAACT2350.010.51029719
GTATTAG2002.5465852E-119.9870191
AGAACCG1451.6184276E-79.8274625
GTATCAA18800.09.6632251
GTCCTAC3100.09.5114471
TACTCTA2201.6370905E-119.4998815
TGGACGG1002.756701E-49.499885
ACCGTCC900.00111515929.4989348
GAACCGT1002.7594622E-49.4989346
GTATAAT3950.09.39104751
TAGGACT3250.09.3542884
CTAGGAC3150.09.3489023
TTAGACT2950.09.3394244
Produced by FastQC (version 0.11.5)