##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513042_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2509389
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.054637204514723	31.0	30.0	34.0	23.0	34.0
2	28.7359683970879	31.0	30.0	34.0	16.0	34.0
3	29.13338745009243	31.0	30.0	34.0	22.0	34.0
4	31.9912644073916	35.0	32.0	37.0	18.0	37.0
5	32.23252672264045	35.0	33.0	37.0	19.0	37.0
6	32.68214573348333	36.0	33.0	37.0	25.0	37.0
7	32.67537277002489	36.0	33.0	37.0	25.0	37.0
8	32.67519224799344	37.0	35.0	37.0	25.0	37.0
9	34.04265460636035	39.0	35.0	39.0	25.0	39.0
10	33.53798793252063	38.0	34.0	39.0	18.0	39.0
11	33.905372582728305	39.0	34.0	39.0	24.0	39.0
12	33.758082545193275	39.0	34.0	39.0	22.0	39.0
13	33.834765355231895	39.0	34.0	39.0	23.0	39.0
14	34.135435358965864	39.0	33.0	41.0	15.0	41.0
15	34.673911458127854	40.0	34.0	41.0	19.0	41.0
16	34.2523347316817	39.0	33.0	41.0	17.0	41.0
17	33.761482177534056	39.0	33.0	41.0	10.0	41.0
18	33.15491460271803	38.0	32.0	41.0	10.0	41.0
19	34.063736232206324	39.0	33.0	41.0	18.0	41.0
20	34.329935295006074	39.0	34.0	41.0	18.0	41.0
21	34.35345137800476	39.0	34.0	41.0	16.0	41.0
22	33.92092218464335	39.0	34.0	41.0	9.0	41.0
23	32.18335060845489	38.0	30.0	41.0	2.0	41.0
24	33.2970595631048	39.0	32.0	41.0	2.0	41.0
25	33.03149372217699	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8536870976523332
1101	2	1.2290935501728946
1101	3	0.9665856471890315
1101	4	1.007154211334118
1101	5	1.2002047070308564
1101	6	0.8703730535987546
1101	7	0.8321108518111231
1101	8	0.7596639501051499
1101	9	1.075809846324013
1101	10	1.3391368071271543
1101	11	0.9906307966413053
1101	12	1.0156929434008717
1101	13	0.9585071740347786
1101	14	-0.04252712576564477
1101	15	0.5637794890280858
1101	16	1.2173000980146327
1101	17	1.914983392333781
1101	18	-4.615221094692082
1101	19	-0.8626630378520588
1101	20	0.2798726661161055
1101	21	0.8230525238147735
1101	22	1.2838901588284273
1101	23	3.1400635469664806
1101	24	2.0453339067581027
1101	25	2.2650392899140357
1102	1	0.9270849621924562
1102	2	1.1401734642055565
1102	3	0.9146027459830535
1102	4	1.5716244283062224
1102	5	1.3588548787765902
1102	6	0.9445208030198842
1102	7	0.8812090084953184
1102	8	0.8546428658169063
1102	9	0.9824199171674621
1102	10	1.3288113274862852
1102	11	1.0337774156420139
1102	12	1.1541125934132168
1102	13	0.9909017370761006
1102	14	1.9282302286344901
1102	15	1.403560793294389
1102	16	1.6369030464523036
1102	17	1.1612336780423718
1102	18	1.5273972012849057
1102	19	1.3011911739956687
1102	20	1.3164332714315847
1102	21	1.2937669409279664
1102	22	1.6745811367529342
1102	23	3.297064572209308
1102	24	2.207035661157235
1102	25	2.5247057896288894
1103	1	0.5504023909913087
1103	2	0.9546671567736205
1103	3	0.6727231663246478
1103	4	1.122053973205773
1103	5	0.9669448266608498
1103	6	0.5915800342499509
1103	7	0.5583296200089336
1103	8	0.5750921019060087
1103	9	0.7352419587568306
1103	10	0.9924463373621606
1103	11	0.7766291733446238
1103	12	0.8662841683201137
1103	13	0.6863262119805142
1103	14	1.4445308904826746
1103	15	0.9977629448241601
1103	16	1.3048763356628754
1103	17	1.6589424063002554
1103	18	1.6764199327219913
1103	19	1.1511866756324736
1103	20	1.0842398083036002
1103	21	1.0436611099524384
1103	22	1.3755243664673529
1103	23	2.8413831394944253
1103	24	1.760030787111738
1103	25	1.9120948689757782
1104	1	0.8184355042271925
1104	2	1.13491285728837
1104	3	0.9379225730624192
1104	4	1.5705488537384014
1104	5	1.2531923829729195
1104	6	1.020218738073602
1104	7	0.9388809789027377
1104	8	0.9617627711942376
1104	9	1.0828607605997576
1104	10	1.4477283589299788
1104	11	0.9866257874526241
1104	12	1.1964385512407958
1104	13	1.0230261631251167
1104	14	1.9019548175819025
1104	15	1.416974633583031
1104	16	1.7807227602550668
1104	17	2.17753920612153
1104	18	2.782430802430177
1104	19	1.9934464403854264
1104	20	1.4331327097553128
1104	21	1.6528273492373131
1104	22	1.8468198343864728
1104	23	3.440846471272245
1104	24	2.4937361568186276
1104	25	2.5778802867515154
1105	1	0.8895430880714308
1105	2	1.2505841071974295
1105	3	0.9144555783599309
1105	4	1.1426243416586175
1105	5	1.2519434659540778
1105	6	1.0015762508213655
1105	7	0.7861448559422115
1105	8	0.764515560490743
1105	9	1.0714428654521768
1105	10	1.4758300500339914
1105	11	1.0366255890438651
1105	12	1.0912166971770532
1105	13	1.0270637814263424
1105	14	1.7482122992456226
1105	15	1.198870958391396
1105	16	1.5973066243318357
1105	17	2.0122975773178666
1105	18	1.9834190764723587
1105	19	1.5849288183805754
1105	20	1.3322496584931045
1105	21	1.1186480670802155
1105	22	1.6289467763122332
1105	23	3.0805299431610322
1105	24	1.9985527940546604
1105	25	2.054572019058412
1106	1	-0.7249139491442271
1106	2	-0.3116681233306622
1106	3	-0.5133910577625649
1106	4	-3.1184205904208007
1106	5	-0.9039941596387209
1106	6	-0.7664608844571461
1106	7	-0.7381683021640519
1106	8	-0.7442144451358139
1106	9	-0.7741089390651439
1106	10	-0.6722143193896883
1106	11	-0.7104116266140466
1106	12	-0.6508321734925033
1106	13	-0.6919298686017896
1106	14	0.05122331122215229
1106	15	-0.5726940283459996
1106	16	-1.5435148530413656
1106	17	-6.2673897468522135
1106	18	-7.332203461908797
1106	19	-3.3501463363497557
1106	20	-2.0466756690529166
1106	21	-1.5614443692191173
1106	22	-1.9612131786753082
1106	23	-5.402033355609163
1106	24	-2.61495335496873
1106	25	-1.131694358242445
1107	1	0.3469516524343099
1107	2	0.7353699981254529
1107	3	0.48566213829745664
1107	4	0.8636734110325577
1107	5	0.8350412142294914
1107	6	0.39134587206307003
1107	7	0.26903695538253203
1107	8	0.31714083707541363
1107	9	0.2765997052283069
1107	10	0.7210519685554004
1107	11	0.35299029033242135
1107	12	0.4496717277640627
1107	13	0.3701133714302429
1107	14	1.1985611213203384
1107	15	0.6555194030093858
1107	16	-5.709537056521999
1107	17	-1.057352299032516
1107	18	0.6236338363317913
1107	19	0.5957110648988575
1107	20	0.4228483829496952
1107	21	0.5242591564984096
1107	22	0.6140190137194921
1107	23	2.529747696642744
1107	24	1.4387532365901308
1107	25	1.160413487234905
1108	1	0.2558620553425328
1108	2	0.490879697623555
1108	3	0.33252492135376954
1108	4	0.8157145216773856
1108	5	0.635971082395308
1108	6	0.2639376928091224
1108	7	0.179951895247072
1108	8	0.19726066625094774
1108	9	0.2204077083049114
1108	10	0.6528662703617414
1108	11	0.32182099767925365
1108	12	0.2629417403277472
1108	13	0.12197232821612403
1108	14	0.7248159626243051
1108	15	0.4719024665982303
1108	16	0.6827638247544812
1108	17	0.8119716009309741
1108	18	0.6112289246738172
1108	19	0.6016128109514369
1108	20	0.3722017497428638
1108	21	0.3700798167996524
1108	22	0.7315401491538793
1108	23	2.5107540642323514
1108	24	1.145114914640331
1108	25	1.2552701742433854
1109	1	0.25100023280223027
1109	2	0.5709841334286594
1109	3	0.32318664269201847
1109	4	0.4268272402093416
1109	5	0.18592077594161793
1109	6	0.10130082292445763
1109	7	0.04065859454802023
1109	8	-0.03642155013946535
1109	9	0.05368825859270743
1109	10	0.4959179246487224
1109	11	0.22955889614596714
1109	12	0.16444641715869324
1109	13	0.13324123568563806
1109	14	-1.1306000818941229
1109	15	-0.2525232313915353
1109	16	-0.3952102620213651
1109	17	0.7373346289278118
1109	18	1.4929744571530605
1109	19	0.6990261362374781
1109	20	0.5222726108178435
1109	21	0.405803290373548
1109	22	0.2307488616948774
1109	23	-4.566534126614034
1109	24	-1.7874836673526424
1109	25	-0.3894807057104188
1110	1	0.32368557808995035
1110	2	0.7031253308658734
1110	3	0.4628930843680443
1110	4	0.9669574250544315
1110	5	0.6485937905524182
1110	6	0.2174030633490034
1110	7	0.16829983001920112
1110	8	0.2036289231757138
1110	9	0.18107866163133224
1110	10	0.5529083445629226
1110	11	0.3174876812131302
1110	12	0.31726390332423904
1110	13	0.1516960848889326
1110	14	0.965538031915024
1110	15	0.5731753951027372
1110	16	0.9104072594294692
1110	17	1.1330519400092456
1110	18	1.7918298583922692
1110	19	0.9599643543683669
1110	20	0.44677013093909324
1110	21	0.39214142451008627
1110	22	0.5761765073410103
1110	23	1.9956743621844808
1110	24	1.1986612925710602
1110	25	1.3598491613113097
1111	1	0.4990551730339412
1111	2	0.7508997486102054
1111	3	0.5240742535951135
1111	4	0.9870950680468056
1111	5	0.7446207400304488
1111	6	0.31149843734144156
1111	7	0.1696628511159659
1111	8	-0.029272652638184127
1111	9	0.1671872552680327
1111	10	0.49007830471506253
1111	11	0.1968501254536008
1111	12	0.27008653058136645
1111	13	0.21340964492368641
1111	14	-0.6173532357059202
1111	15	-0.08931210330449346
1111	16	0.4552121125694839
1111	17	0.9710348826228596
1111	18	1.638898539133507
1111	19	0.674282566461045
1111	20	0.5265435947195982
1111	21	0.4045940234891816
1111	22	0.8284791211691882
1111	23	0.4893493203951067
1111	24	0.5171287944676024
1111	25	0.09704269382903874
1112	1	0.1508709361399454
1112	2	0.5302234251616724
1112	3	0.2899566262525042
1112	4	0.8824561613693476
1112	5	0.659372377439496
1112	6	0.017647818002927806
1112	7	0.1148410705920071
1112	8	0.05870066230450277
1112	9	0.046949605600687505
1112	10	0.3613191959368578
1112	11	0.06547601208197307
1112	12	0.06889089314590535
1112	13	-0.02909549824454416
1112	14	0.38807076906373084
1112	15	0.20939361098266573
1112	16	0.5784008311677198
1112	17	0.7434093783993987
1112	18	0.8786042041600126
1112	19	0.25495184614325694
1112	20	0.12062865924344379
1112	21	0.2759569237129895
1112	22	0.19526750906081247
1112	23	1.9770154925931038
1112	24	0.9496687349586992
1112	25	0.8989273710726948
1113	1	0.2777398260549795
1113	2	0.6781594602859222
1113	3	0.3887876023102024
1113	4	0.5046289689929395
1113	5	0.6247761827332496
1113	6	0.17514323911319707
1113	7	0.017675054383666122
1113	8	-0.0650578094544656
1113	9	0.06226218330173339
1113	10	0.3377249688649542
1113	11	-0.009247566060821555
1113	12	0.022004488133411826
1113	13	-0.02711035380878002
1113	14	0.6617730584335533
1113	15	0.09080274334747429
1113	16	0.39697699032347344
1113	17	0.9100148014257741
1113	18	1.3946844744358273
1113	19	0.5526568480768717
1113	20	0.2767371557917073
1113	21	0.14467949227376664
1113	22	0.4631605744129885
1113	23	0.437378016588184
1113	24	0.3154604889048471
1113	25	0.6176115675119433
1114	1	-0.3092284662381708
1114	2	-3.067514378081551
1114	3	-1.0177952882322288
1114	4	0.2208210091460998
1114	5	0.4501965171793003
1114	6	0.27295313790867226
1114	7	0.1490836293907094
1114	8	0.15971574485862305
1114	9	0.23547382915317172
1114	10	0.4196004993991238
1114	11	0.1570256420811944
1114	12	0.31810271110567356
1114	13	0.2512885839569776
1114	14	1.0719211874189298
1114	15	0.40938175843433555
1114	16	0.7613719991605663
1114	17	1.1325899771941152
1114	18	1.1864776259012615
1114	19	0.6322669755857575
1114	20	0.35099747228154854
1114	21	0.30133914354868807
1114	22	0.48813000136100726
1114	23	2.1192814190511484
1114	24	1.0495793190358782
1114	25	1.0232834012492376
1115	1	0.24126282772984098
1115	2	0.5414260988230666
1115	3	0.304086594703044
1115	4	0.4833585024438847
1115	5	0.4416226567235597
1115	6	0.04321468053475286
1115	7	-0.10589500652354644
1115	8	-0.1238951826559429
1115	9	-0.07474567627299677
1115	10	0.1142970798809202
1115	11	0.07135835202193874
1115	12	0.0059792883621341275
1115	13	-0.13401789669111253
1115	14	0.8939000640645673
1115	15	0.10783133929385968
1115	16	0.5842408088299038
1115	17	1.0606311914606792
1115	18	1.5100085517330442
1115	19	0.6669971362069802
1115	20	0.246458867796008
1115	21	0.23858909708938825
1115	22	0.43633655859127884
1115	23	2.2160547136772877
1115	24	1.026999231206382
1115	25	0.697539988057045
1116	1	0.6756293052955513
1116	2	0.6346808745345918
1116	3	0.7114969092972672
1116	4	1.3907253213119688
1116	5	0.867943311373665
1116	6	0.718651589449081
1116	7	0.6152593034138576
1116	8	0.6703735783834261
1116	9	0.7412479009090589
1116	10	1.1420704612178838
1116	11	0.7068066413500063
1116	12	0.8176066649083964
1116	13	0.8603584696569726
1116	14	1.5788661743833003
1116	15	0.8420497113139334
1116	16	1.2075044872576584
1116	17	1.536625249143654
1116	18	2.160442050985914
1116	19	1.258195875362773
1116	20	1.1120077401502044
1116	21	0.9698646149131918
1116	22	1.3792039594706935
1116	23	2.6129423636970444
1116	24	1.5296854820147772
1116	25	1.808127948183298
1201	1	1.1128520786589
1201	2	1.4493044901899665
1201	3	1.1663819009824579
1201	4	1.8670652699286592
1201	5	1.6112805318417784
1201	6	1.1599176984033193
1201	7	1.1098246676916403
1201	8	1.0823880045024268
1201	9	1.3257523428945603
1201	10	1.6846721467558368
1201	11	1.2950676533087133
1201	12	1.4642798260313228
1201	13	1.3515728693847961
1201	14	2.3642168432248525
1201	15	1.685335580267413
1201	16	2.010387604533072
1201	17	2.5904978369738245
1201	18	2.9959969732591176
1201	19	2.0997754590816413
1201	20	1.8121311904632407
1201	21	1.7606336237176734
1201	22	2.077338532235636
1201	23	3.7070124163840106
1201	24	2.5979060884396006
1201	25	2.727161275793243
1202	1	1.0647795613152837
1202	2	1.2441266777879711
1202	3	1.0257187063758266
1202	4	1.602752614369912
1202	5	1.417356331071737
1202	6	0.9923552135560527
1202	7	0.8642744262489046
1202	8	0.9757542234268257
1202	9	1.0258596909391144
1202	10	1.5111062621367708
1202	11	1.0941074429832796
1202	12	1.1412749538035811
1202	13	1.0623218812600825
1202	14	1.9406366399725528
1202	15	1.3219293637007254
1202	16	1.6657449142092418
1202	17	2.0431131700891783
1202	18	2.802967756574546
1202	19	2.047217317089732
1202	20	1.668387960535462
1202	21	1.556804265125713
1202	22	1.990599588444745
1202	23	3.576317353782393
1202	24	2.2560823616580308
1202	25	2.516145353473078
1203	1	1.0328397913681329
1203	2	1.3975856770561421
1203	3	1.0750311932212604
1203	4	1.699604980067825
1203	5	1.536653042092226
1203	6	1.1230309865618153
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2211	17	-0.18949793310778063
2211	18	-5.6050846116203665
2211	19	-2.1142278696802244
2211	20	-1.3483525703283092
2211	21	-1.0148675953395525
2211	22	-0.7457653608656614
2211	23	-5.3682212143572485
2211	24	-2.6637459574221722
2211	25	-1.7001803518583394
2212	1	0.08485629806818551
2212	2	0.3642095534811034
2212	3	0.16222578283899836
2212	4	0.5841855230932111
2212	5	0.5930210381708889
2212	6	0.007554829205012936
2212	7	-0.04759094412270315
2212	8	-0.1720212781979953
2212	9	-0.34225398621936165
2212	10	-3.7591780642146304
2212	11	-1.2811348783368572
2212	12	-0.3838108912682543
2212	13	-0.2185642614168941
2212	14	0.5130776027185249
2212	15	0.034375664655598825
2212	16	0.256115452634333
2212	17	0.7648227314885929
2212	18	1.3302058340186136
2212	19	0.6086256700521062
2212	20	0.13983161240416564
2212	21	0.033049235532018884
2212	22	0.12723304700356408
2212	23	1.5091115725021567
2212	24	0.7122204344311598
2212	25	0.6947667610253063
2213	1	-1.520723362345624
2213	2	-3.9569915801674114
2213	3	-1.8039773842597775
2213	4	-3.3176768631606635
2213	5	-1.1572556381546661
2213	6	-0.8516983861882075
2213	7	-0.8305094536036641
2213	8	-0.791001121622326
2213	9	-0.7859698623822382
2213	10	-0.8280941081371509
2213	11	-0.8679673982900908
2213	12	-0.7114547578739163
2213	13	-1.0234652578075156
2213	14	-6.249256035632349
2213	15	-2.749470098142428
2213	16	-1.900038911658278
2213	17	-0.6734662472110173
2213	18	-0.012003382594677703
2213	19	-0.6283713051906332
2213	20	-0.7671193560265195
2213	21	-1.024392556954382
2213	22	-0.8544900479077242
2213	23	-5.773981178247677
2213	24	-3.1544546202178125
2213	25	-2.2013184659585754
2214	1	-0.06928475075640961
2214	2	0.15544156857663083
2214	3	-0.010704478974037812
2214	4	0.3117961843087187
2214	5	0.0948356462375628
2214	6	-0.09195456618770947
2214	7	-0.15861067837854392
2214	8	-0.28723631082634427
2214	9	-0.08799972094693231
2214	10	0.2711828132128389
2214	11	-0.19725878744920777
2214	12	0.00937882490440245
2214	13	-0.16537769014556858
2214	14	0.47704332329404053
2214	15	0.15243840543710974
2214	16	0.4720239360056695
2214	17	0.6124679866187108
2214	18	1.276715779769063
2214	19	0.3024459389411689
2214	20	0.08141035287118825
2214	21	-0.01558509106598649
2214	22	0.5570404217971827
2214	23	2.0748761766377015
2214	24	0.904507563922472
2214	25	0.7729380009026556
2215	1	-2.4390203034547433
2215	2	-4.395238669717539
2215	3	-2.5380324930432465
2215	4	-4.006798345441037
2215	5	-4.796861678467703
2215	6	-2.4054592823635197
2215	7	-1.641819642058774
2215	8	-2.0531474324799603
2215	9	-1.7555999028554936
2215	10	-3.295181931578817
2215	11	-2.195242382439776
2215	12	-1.6966633494770136
2215	13	-1.834199620703032
2215	14	-1.0669665517420341
2215	15	-1.7339609684590158
2215	16	-2.5283162511304944
2215	17	-6.983328354360118
2215	18	-3.314214477033243
2215	19	-2.3384857229687768
2215	20	-2.0491457222552327
2215	21	-1.9285927315276439
2215	22	-1.7616214926846467
2215	23	-6.470122652439262
2215	24	-3.793175277598003
2215	25	-3.500352292825273
2216	1	-0.8799480160625315
2216	2	-0.36407706496374104
2216	3	-0.5645554106510566
2216	4	-2.943225998477441
2216	5	-1.2205813901422076
2216	6	-0.8425956487076753
2216	7	-0.8563813083696665
2216	8	-0.6197518387766578
2216	9	-0.767547192109312
2216	10	-0.4233824041784615
2216	11	-0.9644615381146906
2216	12	-1.725197483879981
2216	13	-1.097092419515576
2216	14	-6.775847093742339
2216	15	-3.1335021446959885
2216	16	-1.6043469601788942
2216	17	-0.5992356513582209
2216	18	0.27110353487110217
2216	19	-0.24128520569059475
2216	20	-0.7697963906958023
2216	21	-1.064373910941761
2216	22	-0.5319990635621821
2216	23	0.7988868243750602
2216	24	-0.09731586109970891
2216	25	0.02995663444303176
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	130644.0
3	3.0
4	1108.0
5	6514.0
6	7665.0
7	6660.0
8	6304.0
9	5839.0
10	5593.0
11	5366.0
12	5262.0
13	5365.0
14	5448.0
15	5509.0
16	5670.0
17	6106.0
18	6903.0
19	8273.0
20	9995.0
21	12454.0
22	15092.0
23	18608.0
24	22045.0
25	25644.0
26	29602.0
27	33593.0
28	37872.0
29	45311.0
30	53244.0
31	61090.0
32	78759.0
33	103601.0
34	135358.0
35	177437.0
36	249647.0
37	344880.0
38	830925.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.48686872877678	17.66957036535859	12.143274312559042	25.700286593305588
2	14.962936029416749	19.550392288429574	40.26818104074146	25.218490641412217
3	18.95632442887616	23.61819862636081	29.28935359867715	28.136123346085878
4	11.230503746477648	15.93551522090007	38.72307636500184	34.110904667620446
5	13.130764299928563	36.98619597663027	36.11306000612307	13.769979717318092
6	33.19393647584762	36.06770127513847	17.60485853583876	13.133503713175154
7	28.811958930535848	31.076877700365095	21.63225611019881	18.478907258900243
8	26.19088522245655	35.18995131987977	20.07635440785674	18.54280904980694
9	26.49489553698807	15.065490433840493	19.16576280739686	39.27385122177458
10	14.358950031751624	28.22386629954639	34.78471281820889	22.632470850493096
11	35.52590462099665	22.110083478948507	23.4313817711057	18.932630128949146
12	23.14754474721664	24.147573843320274	31.039563925136125	21.665317484326966
13	28.86632493920926	21.10292596662358	25.399588763248715	24.631160330918444
14	21.58977357562539	20.20875508644718	27.87371062624533	30.3277607116821
15	24.007177696209826	28.362900940378648	23.25807939230327	24.371841971108257
16	23.767383815269895	27.241057830394627	25.769758588624125	23.221799765711353
17	22.65702042850025	26.916352765321378	27.243647234678626	23.18297957149975
18	22.39548810745638	26.900006975098893	28.062616459240513	22.64188845820421
19	23.788377319740505	26.58944629188329	26.951364478202393	22.67081191017381
20	24.02741982157505	26.408159621052263	26.817432106127047	22.746988451245635
21	24.19999043336841	26.020919034407985	26.60886826748302	23.170222264740584
22	23.8557323408693	26.159934367256344	26.80242885799664	23.18190443387772
23	22.783326683150616	25.624635518831845	28.330495877750376	23.261541920267163
24	23.543660195285575	26.24698566313816	26.592420993378724	23.616933148197536
25	23.68583858785299	25.956157813375725	26.90787097679454	23.450132621976742
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	248.0
1	248.0
2	363.0
3	478.0
4	478.0
5	478.0
6	958.5
7	1439.0
8	1439.0
9	1439.0
10	2439.5
11	3440.0
12	3440.0
13	3440.0
14	6216.5
15	8993.0
16	8993.0
17	8993.0
18	16459.5
19	23926.0
20	23926.0
21	23926.0
22	37504.0
23	51082.0
24	51082.0
25	51082.0
26	71603.5
27	92125.0
28	92125.0
29	92125.0
30	118197.5
31	144270.0
32	144270.0
33	144270.0
34	173088.5
35	201907.0
36	201907.0
37	201907.0
38	230878.5
39	259850.0
40	259850.0
41	259850.0
42	282709.0
43	305568.0
44	305568.0
45	305568.0
46	317838.0
47	330108.0
48	330108.0
49	330108.0
50	329432.5
51	328757.0
52	328757.0
53	328757.0
54	305604.0
55	282451.0
56	282451.0
57	282451.0
58	245664.5
59	208878.0
60	208878.0
61	208878.0
62	172031.0
63	135184.0
64	135184.0
65	135184.0
66	105103.5
67	75023.0
68	75023.0
69	75023.0
70	54903.0
71	34783.0
72	34783.0
73	34783.0
74	24282.0
75	13781.0
76	13781.0
77	13781.0
78	9312.5
79	4844.0
80	4844.0
81	4844.0
82	3228.5
83	1613.0
84	1613.0
85	1613.0
86	1038.5
87	464.0
88	464.0
89	464.0
90	292.5
91	121.0
92	121.0
93	121.0
94	77.5
95	34.0
96	34.0
97	34.0
98	28.0
99	22.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1076357631279965
2	0.04614669148545721
3	0.03466979412119842
4	0.030525358961882753
5	0.033673535669439855
6	0.019327413964116365
7	0.01868980855499088
8	0.022395889995532776
9	0.0279749373253808
10	0.03411188938821362
11	0.01964621666867911
12	0.018410856188498474
13	0.014824325762167603
14	0.020562774444296997
15	0.02016427106359357
16	0.020642475120437685
17	0.025464366026949188
18	0.01849055686463916
19	0.03231862417504819
20	0.02179813492447763
21	0.02665987616905948
22	0.035267549192253576
23	0.027337331916255307
24	0.038256324547529294
25	0.03008700524310898
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2509389.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.59085937897156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.15120388735255	55.08200299401058
2	12.809145494024857	17.827988856789418
3	4.083743422791067	8.525736428257595
4	1.7057673355114624	4.748232591152847
5	0.8273661902398646	2.8788562099948916
6	0.45094676344981116	1.8829063681582898
7	0.2783422162856576	1.3559051822936539
8	0.16206310581293837	0.9022488645718066
9	0.11478626853201278	0.7189267564843352
>10	0.3980435170359363	4.367505958760542
>50	0.012575860131636583	0.5862671338010897
>100	0.00520843725284012	0.6348094883271911
>500	5.767868424013859E-4	0.24312248049101143
>1k	2.3071473694445433E-4	0.24549068690682282
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	3.985033807034302E-5
11	0.0	0.0	0.0	0.0	3.985033807034302E-5
12	0.0	0.0	0.0	0.0	3.985033807034302E-5
13	0.0	0.0	0.0	0.0	3.985033807034302E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGTAG	50	0.0015014904	13.298242	10
GGTATCA	980	0.0	13.199559	1
ATACCGT	75	1.4811489E-5	12.665244	6
CGTGTAA	70	1.09276305E-4	12.212671	10
CGAAATC	65	8.031312E-4	11.691462	13
GCGCTAG	60	0.0058249184	11.096687	1
CTACGAC	60	0.0058787875	11.082973	3
TCGAACT	235	0.0	10.510297	19
GTATTAG	200	2.5465852E-11	9.987019	1
AGAACCG	145	1.6184276E-7	9.827462	5
GTATCAA	1880	0.0	9.663225	1
GTCCTAC	310	0.0	9.511447	1
TACTCTA	220	1.6370905E-11	9.499881	5
TGGACGG	100	2.756701E-4	9.49988	5
ACCGTCC	90	0.0011151592	9.498934	8
GAACCGT	100	2.7594622E-4	9.498934	6
GTATAAT	395	0.0	9.3910475	1
TAGGACT	325	0.0	9.354288	4
CTAGGAC	315	0.0	9.348902	3
TTAGACT	295	0.0	9.339424	4
>>END_MODULE