FastQCFastQC Report
Thu 26 May 2016
SRR1513042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513042_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2509389
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38830.15473886272714196No Hit
TATCAACGCAGAGTACTTTTTTTTT26500.10560339588640899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8250.013.9754231
TAGGACG2550.010.828974
GTATCAA20250.010.8227191
TAGGCTG3250.010.5474755
GTCTTAC2201.8189894E-129.9618211
GTATTGG2401.8189894E-129.5286981
TTAGGAC3000.09.5220263
TAAGGGT2600.09.5220264
GCGTGCG1106.937023E-59.4876719
GTATAAG3550.09.394491
CTCTTAC3450.09.39061
ATTATAC2950.09.3606353
TGGCGAG2550.09.30238118
AGGACTG5950.09.2819755
GTAGGAC3800.09.2714463
TAGGACT3850.09.1510384
CCGTGCG1054.5526007E-49.0358789
AAGGCGT1804.0650775E-88.9930256
TTACACT3500.08.977914
TACGCTG850.0072974528.9619065
Produced by FastQC (version 0.11.5)