##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513042_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2509389 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.444856098436713 31.0 31.0 34.0 26.0 34.0 2 30.60710435886983 31.0 31.0 34.0 27.0 34.0 3 30.596530470166243 31.0 31.0 34.0 27.0 34.0 4 33.83588315721477 37.0 35.0 37.0 30.0 37.0 5 33.75376954310392 37.0 35.0 37.0 28.0 37.0 6 33.70992699816569 37.0 35.0 37.0 29.0 37.0 7 33.62714150735498 37.0 35.0 37.0 28.0 37.0 8 33.614659982967964 37.0 35.0 37.0 28.0 37.0 9 35.17426234035457 39.0 35.0 39.0 28.0 39.0 10 35.07949783791991 39.0 35.0 39.0 28.0 39.0 11 35.08624728967888 39.0 35.0 39.0 28.0 39.0 12 35.001979366291955 39.0 35.0 39.0 27.0 39.0 13 34.97140618692438 39.0 35.0 39.0 27.0 39.0 14 36.153018125129265 40.0 36.0 41.0 27.0 41.0 15 36.12750673570339 40.0 36.0 41.0 27.0 41.0 16 36.09612618848652 40.0 36.0 41.0 27.0 41.0 17 35.99191356939877 40.0 36.0 41.0 27.0 41.0 18 35.91585760517799 40.0 36.0 41.0 27.0 41.0 19 35.9938148290281 40.0 37.0 41.0 27.0 41.0 20 35.87685727481869 40.0 36.0 41.0 27.0 41.0 21 35.89571046975977 40.0 36.0 41.0 27.0 41.0 22 35.86092152312774 40.0 36.0 41.0 27.0 41.0 23 35.79236937756561 40.0 36.0 41.0 27.0 41.0 24 35.657918321950085 40.0 36.0 41.0 26.0 41.0 25 35.63405474400342 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.737014228269036 1101 2 0.6210962196658087 1101 3 0.720994187246955 1101 4 0.9559942686550968 1101 5 0.6845844453848287 1101 6 0.7385768111410229 1101 7 0.605676434495173 1101 8 0.6331006441882892 1101 9 0.667950033297025 1101 10 0.7076472718366134 1101 11 0.6792646862441316 1101 12 0.7296859418542994 1101 13 0.7001637131742271 1101 14 0.8045842461789405 1101 15 0.8331946284335743 1101 16 0.802028527051597 1101 17 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4 0.4222057694328569 2210 5 0.08868059180855425 2210 6 0.037460028692294145 2210 7 0.051408536140428396 2210 8 0.06371441698760094 2210 9 -0.0564398872749976 2210 10 -0.06864039776837672 2210 11 -0.13093721650026424 2210 12 -0.11689875105268754 2210 13 -0.14603028149662833 2210 14 -0.2104604351830517 2210 15 -0.1508472614555103 2210 16 -0.22594265405476222 2210 17 -0.13202814651504724 2210 18 -0.18393638949824265 2210 19 -0.04427893555213558 2210 20 -0.10070553046164576 2210 21 -0.1105097584488135 2210 22 -0.16283336600281473 2210 23 -0.24112686860487997 2210 24 -0.0355115548548568 2210 25 0.04096994875974502 2211 1 0.24238528988427888 2211 2 0.13499970348550505 2211 3 0.2875256245590805 2211 4 0.6243586056189798 2211 5 0.21763908534197896 2211 6 0.09803728359524655 2211 7 -0.007977035681776101 2211 8 -0.06111629942351726 2211 9 -3.2234988425727806E-4 2211 10 -0.013473701631745882 2211 11 -0.0699970856536325 2211 12 -0.10358374708768991 2211 13 -0.024213156168727323 2211 14 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-0.45813077178018347 2214 11 -0.5873303777337924 2214 12 -0.5050864968691755 2214 13 -0.4842229022296962 2214 14 -0.48127874278240057 2214 15 -0.43930267016756375 2214 16 -0.5697790714170381 2214 17 -0.5222727600477555 2214 18 -0.5786037671559754 2214 19 -0.564910308243455 2214 20 -0.49991184986807014 2214 21 -0.6088313041602262 2214 22 -0.5684350711192678 2214 23 -0.716992497603222 2214 24 -0.5096260002266106 2214 25 -0.6549262549581272 2215 1 -0.43212137191941125 2215 2 -0.4036285190065918 2215 3 -0.39080639053997857 2215 4 -0.09668977383135058 2215 5 -0.5347072757407147 2215 6 -0.597210317758595 2215 7 -0.7907702160837786 2215 8 -0.9128524760634775 2215 9 -0.9522345569961814 2215 10 -0.969725025041015 2215 11 -1.0628354232789263 2215 12 -0.9680610644208585 2215 13 -1.1012203533946519 2215 14 -1.300505994014138 2215 15 -1.2437956302761108 2215 16 -1.2505380712825698 2215 17 -1.2852086795849473 2215 18 -1.2461068908764616 2215 19 -1.2296689696744565 2215 20 -1.131604188707513 2215 21 -1.1664281772669227 2215 22 -1.219911809033455 2215 23 -1.3577831048363507 2215 24 -1.2695954202495443 2215 25 -1.3814469213719747 2216 1 -1.4927651027758984 2216 2 -0.9367967485947055 2216 3 -2.779124628319156 2216 4 -1.1070751758637272 2216 5 -1.2015145350102685 2216 6 -1.1852921316079446 2216 7 -1.2175594053668846 2216 8 -1.260113681688999 2216 9 -1.3053910336466785 2216 10 -1.3624342322948237 2216 11 -1.2940961275119705 2216 12 -1.3454303300546258 2216 13 -1.3268604355038747 2216 14 -1.5055533656750342 2216 15 -1.563444196343255 2216 16 -1.5965914050461407 2216 17 -1.4800322986458028 2216 18 -1.7034107769163995 2216 19 -1.655879695998557 2216 20 -1.6674185047039174 2216 21 -1.7139111621903993 2216 22 -1.73878334086195 2216 23 -1.7751247070087217 2216 24 -1.68447293900212 2216 25 -1.7618428476821748 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70802.0 3 2.0 4 1177.0 5 9081.0 6 10120.0 7 7646.0 8 6249.0 9 5365.0 10 4777.0 11 4455.0 12 4089.0 13 3906.0 14 3722.0 15 3825.0 16 3920.0 17 4269.0 18 4606.0 19 5433.0 20 6814.0 21 8959.0 22 11329.0 23 13376.0 24 15614.0 25 17776.0 26 19026.0 27 21035.0 28 23856.0 29 28587.0 30 36315.0 31 46084.0 32 58456.0 33 76236.0 34 101413.0 35 138085.0 36 202267.0 37 334644.0 38 1196073.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.18967227173928 18.60829278186531 13.309838235975208 27.892196710420198 2 14.527535191609841 20.02117478167245 38.20831837939148 27.242971647326236 3 16.92443857847468 23.666677426257944 29.74333592759034 29.665548067677033 4 10.7565228029612 16.61974289358884 37.29501484225841 35.32871946119155 5 13.516944558704894 37.24522577333065 35.406323438909986 13.831506229054465 6 33.369255804799366 36.52799312592707 17.036972143683485 13.06577892559008 7 28.5511501755829 31.24901369116219 21.55019056682272 18.649645566432184 8 26.066629380598144 35.54683155453805 19.76752946841839 18.619009596445412 9 26.173850785490586 14.829563212379052 18.983446654888027 40.01313934724233 10 14.380891313106517 28.63640946571347 34.341406339354776 22.641292881825237 11 35.57168675539048 22.030498727725227 23.263160621451927 19.134653895432365 12 22.91516299082271 24.355901988299525 30.897773689595077 21.83116133128269 13 28.792782007146574 21.02791595959467 25.42541888286743 24.753883150391324 14 21.745577634340727 20.310064729987523 27.143715078066126 30.800642557605624 15 23.825181209594216 28.55601786412379 23.14174736698644 24.477053559295555 16 23.81570897641561 27.479848151211105 25.21208014905892 23.49236272331437 17 22.87602671962333 27.238414201994804 26.433222068004454 23.452337010377413 18 22.751924290120183 27.287634217790323 26.984764391600518 22.97567710048898 19 23.728783155679217 26.60658051040014 26.845835558992594 22.818800774928047 20 24.014092673555197 26.48477378357839 26.609505341738565 22.891628201127844 21 24.097618982150635 26.058773669606428 26.508644136082527 23.33496321216041 22 23.731872579341026 26.32170620019455 26.51358557800325 23.43283564246117 23 23.744740236735673 26.04435117664476 26.318562227099036 23.892346359520538 24 23.343188345501666 26.314755459808246 26.67145668875576 23.670599505934327 25 23.69030867673366 26.14867603229312 26.542676324794602 23.61833896617862 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 148.0 1 148.0 2 275.0 3 402.0 4 402.0 5 402.0 6 999.0 7 1596.0 8 1596.0 9 1596.0 10 2503.0 11 3410.0 12 3410.0 13 3410.0 14 6139.5 15 8869.0 16 8869.0 17 8869.0 18 16035.0 19 23201.0 20 23201.0 21 23201.0 22 36479.5 23 49758.0 24 49758.0 25 49758.0 26 70656.5 27 91555.0 28 91555.0 29 91555.0 30 116324.0 31 141093.0 32 141093.0 33 141093.0 34 169219.5 35 197346.0 36 197346.0 37 197346.0 38 226117.5 39 254889.0 40 254889.0 41 254889.0 42 277882.5 43 300876.0 44 300876.0 45 300876.0 46 315156.0 47 329436.0 48 329436.0 49 329436.0 50 331002.5 51 332569.0 52 332569.0 53 332569.0 54 309691.5 55 286814.0 56 286814.0 57 286814.0 58 250464.0 59 214114.0 60 214114.0 61 214114.0 62 176529.5 63 138945.0 64 138945.0 65 138945.0 66 108211.5 67 77478.0 68 77478.0 69 77478.0 70 56793.5 71 36109.0 72 36109.0 73 36109.0 74 25009.0 75 13909.0 76 13909.0 77 13909.0 78 9390.0 79 4871.0 80 4871.0 81 4871.0 82 3164.5 83 1458.0 84 1458.0 85 1458.0 86 950.5 87 443.0 88 443.0 89 443.0 90 269.0 91 95.0 92 95.0 93 95.0 94 50.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18940865684834038 2 0.06730722100080937 3 0.0 4 0.0 5 0.019965019373241855 6 0.38100908229054964 7 0.0013150611563213198 8 0.03411188938821362 9 0.03558635189681632 10 0.0031880270456274415 11 0.0041444351593156745 12 0.010361087898289184 13 0.008567822685123749 14 0.0016737141989544067 15 7.970067614068604E-5 16 0.01251300615408771 17 0.026739576845200164 18 0.06818392843835691 19 0.030644909976093784 20 0.0 21 0.0 22 0.0 23 3.1880270456274417E-4 24 2.3910202842205813E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2509389.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.49904764088049 #Duplication Level Percentage of deduplicated Percentage of total 1 73.53596317534382 45.95927665813847 2 15.290868964783424 19.113294958009202 3 5.484188486516562 10.282696724711005 4 2.3354872033735314 5.838629039532363 5 1.2046613545946716 3.764509369597 6 0.6711075594647852 2.5166149998686786 7 0.4031912839103756 1.7639349883051634 8 0.27008181762987105 1.3503885109587923 9 0.17320597451668085 0.974268760770285 >10 0.6033411099493992 5.947793542668521 >50 0.01933453448285303 0.8255559786161082 >100 0.007547811332741605 0.8828323726221627 >500 6.37952563317768E-4 0.2820504791775964 >1k 3.827715379889621E-4 0.4981536170246423 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3883 0.15473886272714196 No Hit TATCAACGCAGAGTACTTTTTTTTT 2650 0.10560339588640899 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 3.985033807034302E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 825 0.0 13.975423 1 TAGGACG 255 0.0 10.82897 4 GTATCAA 2025 0.0 10.822719 1 TAGGCTG 325 0.0 10.547475 5 GTCTTAC 220 1.8189894E-12 9.961821 1 GTATTGG 240 1.8189894E-12 9.528698 1 TTAGGAC 300 0.0 9.522026 3 TAAGGGT 260 0.0 9.522026 4 GCGTGCG 110 6.937023E-5 9.487671 9 GTATAAG 355 0.0 9.39449 1 CTCTTAC 345 0.0 9.3906 1 ATTATAC 295 0.0 9.360635 3 TGGCGAG 255 0.0 9.302381 18 AGGACTG 595 0.0 9.281975 5 GTAGGAC 380 0.0 9.271446 3 TAGGACT 385 0.0 9.151038 4 CCGTGCG 105 4.5526007E-4 9.035878 9 AAGGCGT 180 4.0650775E-8 8.993025 6 TTACACT 350 0.0 8.97791 4 TACGCTG 85 0.007297452 8.961906 5 >>END_MODULE