FastQCFastQC Report
Thu 26 May 2016
SRR1513041_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513041_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2253304
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC48670.2159939360157351No Hit
CCCATGTACTCTGCGTTGATACCAC38490.1708158331055197No Hit
GTATCAACGCAGAGTACTTTTTTTT22710.10078533566709152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6550.013.3457734
TAAGACG550.003065288812.0929584
GCGTGCG1001.9339423E-611.3983899
GTAGGAC12550.011.2805453
GTCCTAC12700.011.0865281
TAAGACT2750.011.0564194
GGCGTGC1303.2750904E-810.9599898
TGTAGGA13800.010.9482362
GACCGGG700.001493613610.8556087
GTCCTAA5450.010.6480681
GTTCTAG2250.010.5704881
CTACACT5400.010.5573444
CGTGCGC1002.4034322E-510.44852310
GGACCTG9050.010.18110756
AGGACCT10000.010.1662715
TCCTACA15250.010.1564952
TGGCGAG10350.010.09586318
TACACGG856.587816E-410.06008155
GTCTTAC1909.640644E-1110.0141471
CTGTAGG14450.010.0072161
Produced by FastQC (version 0.11.5)