##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513041_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2253304 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.259237546287586 31.0 30.0 34.0 25.0 34.0 2 28.941528972566505 31.0 30.0 34.0 16.0 34.0 3 29.31978774279902 31.0 30.0 34.0 23.0 34.0 4 32.18425254648285 35.0 33.0 37.0 19.0 37.0 5 32.44569130485722 35.0 33.0 37.0 22.0 37.0 6 32.88615473100833 37.0 33.0 37.0 26.0 37.0 7 32.88407733710143 37.0 35.0 37.0 26.0 37.0 8 32.86548375185949 37.0 35.0 37.0 26.0 37.0 9 34.24233259249529 39.0 35.0 39.0 26.0 39.0 10 33.75693381807337 39.0 34.0 39.0 20.0 39.0 11 34.116760099835616 39.0 34.0 39.0 25.0 39.0 12 33.95446553150396 39.0 34.0 39.0 24.0 39.0 13 34.04435087320663 39.0 35.0 39.0 25.0 39.0 14 34.33966743945779 39.0 33.0 41.0 17.0 41.0 15 34.896145837401434 40.0 34.0 41.0 23.0 41.0 16 34.49206498546135 39.0 34.0 41.0 17.0 41.0 17 34.03951974522745 39.0 33.0 41.0 16.0 41.0 18 33.46373547466298 39.0 32.0 41.0 10.0 41.0 19 34.35052971103766 39.0 34.0 41.0 18.0 41.0 20 34.60308950767406 39.0 34.0 41.0 18.0 41.0 21 34.623875429147596 40.0 34.0 41.0 18.0 41.0 22 34.19989979159492 39.0 34.0 41.0 10.0 41.0 23 32.536583168538286 39.0 31.0 41.0 2.0 41.0 24 33.631868580537734 39.0 33.0 41.0 2.0 41.0 25 33.36274643811931 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5714109717070741 1101 2 0.9490043668741279 1101 3 0.7269887939007553 1101 4 0.6278079874285822 1101 5 0.8281377208367076 1101 6 0.511261367889027 1101 7 0.5456761073622545 1101 8 0.6522523611825761 1101 9 0.8448261767821066 1101 10 1.1876742671327705 1101 11 0.7651129079374925 1101 12 0.8335922763481207 1101 13 0.7812316591261634 1101 14 -0.102622585053318 1101 15 0.5176553863383688 1101 16 1.0953653406767572 1101 17 1.6238406283995275 1101 18 -4.502274504842031 1101 19 -0.7430224407772883 1101 20 0.24641895600976227 1101 21 0.5903427457174857 1101 22 1.235956655386346 1101 23 2.9127159281158725 1101 24 1.939086150019648 1101 25 2.0886088197786634 1102 1 0.8956962444795735 1102 2 1.1874710250568157 1102 3 1.0544453604342934 1102 4 1.551038645396659 1102 5 1.3268660550960334 1102 6 1.0187448141523987 1102 7 0.9230605083393613 1102 8 0.8994712048398341 1102 9 0.9600977387640057 1102 10 1.305914128149226 1102 11 0.9700224715254393 1102 12 1.0820764449321842 1102 13 0.9076830958082596 1102 14 1.773073164073928 1102 15 1.224703577854008 1102 16 1.5122878643561464 1102 17 1.2203417407024375 1102 18 1.5215763556684863 1102 19 1.2751975109704503 1102 20 1.09518770741483 1102 21 1.223931145309372 1102 22 1.6534464823708106 1102 23 3.1445217165251265 1102 24 2.0900722796001645 1102 25 2.3130072073496066 1103 1 0.8593794271936375 1103 2 1.206583057087208 1103 3 0.9109690403667052 1103 4 1.3281171584964255 1103 5 1.2444453171671768 1103 6 0.8918193154694691 1103 7 0.7163484900056645 1103 8 0.6649286415675917 1103 9 0.887192121931129 1103 10 1.0177078502734815 1103 11 0.848262329363699 1103 12 0.9820142953000683 1103 13 0.8288860380424552 1103 14 1.5137452636439193 1103 15 1.114982509590007 1103 16 1.3448049847790031 1103 17 1.811286452368421 1103 18 1.7371330313249373 1103 19 1.330211644999693 1103 20 1.260098553829657 1103 21 1.1255996225298333 1103 22 1.466269550014914 1103 23 2.9390974002464887 1103 24 1.8408718595445848 1103 25 2.0464103015603214 1104 1 0.46228660211826167 1104 2 0.8938883227855214 1104 3 0.6548787793058146 1104 4 1.1852352273557614 1104 5 1.0181317111551778 1104 6 0.5763305892940593 1104 7 0.48480309655583653 1104 8 0.5649592053953469 1104 9 0.53024378360805 1104 10 0.8898960882203681 1104 11 0.4964290722197333 1104 12 0.6240727646952848 1104 13 0.4887697369526407 1104 14 1.3295981133465204 1104 15 0.8115829663739049 1104 16 1.119112167983161 1104 17 1.5668838166566559 1104 18 2.1712302841917293 1104 19 1.3084358425670928 1104 20 0.799021280193557 1104 21 0.8563554651093881 1104 22 1.1662126502087773 1104 23 2.6393027151816284 1104 24 1.5888119720582239 1104 25 1.701020903421103 1105 1 0.7736077712273932 1105 2 1.0828470715097396 1105 3 0.7356678030816042 1105 4 0.9841426865200518 1105 5 1.0035966546763717 1105 6 0.6755903142855502 1105 7 0.5918458292429207 1105 8 0.5742003696812716 1105 9 0.6555685959554438 1105 10 1.0722375793641987 1105 11 0.7119825989691151 1105 12 0.7214759884874411 1105 13 0.7192902549298239 1105 14 1.4937819806051778 1105 15 0.8784023676942496 1105 16 1.2144491675223748 1105 17 1.6894391650251634 1105 18 1.591904584720126 1105 19 1.077084183946269 1105 20 0.8767096756614734 1105 21 0.6256583835757823 1105 22 1.2434760012128194 1105 23 2.6855266236097393 1105 24 1.6757639252387406 1105 25 1.7052377579198108 1106 1 -0.6389095117578663 1106 2 -0.09100016847734693 1106 3 -0.2847353543752398 1106 4 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-5.383328491173824 1107 17 -0.743490872502278 1107 18 0.9820576919956991 1107 19 0.7662697162046328 1107 20 0.7487354039802057 1107 21 0.8336084742545395 1107 22 0.843188140109568 1107 23 2.5998500160503255 1107 24 1.7610743011327585 1107 25 1.3679874594250947 1108 1 0.33221966826372906 1108 2 0.5328286230232351 1108 3 0.25092879639539944 1108 4 0.7352444031158427 1108 5 0.5408581225004383 1108 6 0.2670236308168299 1108 7 0.21302426959014298 1108 8 0.18118605585098635 1108 9 0.23047713452290708 1108 10 0.6381815044084647 1108 11 0.3314176784495828 1108 12 0.363528490262631 1108 13 0.1749145103413099 1108 14 0.7244318823680373 1108 15 0.4497009131767413 1108 16 0.6592762878951604 1108 17 0.765762791161734 1108 18 0.35832732608923834 1108 19 0.5198046306951554 1108 20 0.502609408770553 1108 21 0.44100786207886244 1108 22 0.7204611554582101 1108 23 2.4165158593189133 1108 24 1.3214352855043288 1108 25 1.4669219823108222 1109 1 0.22492604444165565 1109 2 0.6270540037325532 1109 3 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0.45047926836418384 2212 21 0.2853125705688271 2212 22 0.62308515746372 2212 23 1.9540108772829186 2212 24 1.0522209295397502 2212 25 0.8920287402250437 2213 1 -1.5754841405224056 2213 2 -4.036960159946322 2213 3 -1.8479038890564077 2213 4 -3.480361788029551 2213 5 -1.3835348082803627 2213 6 -1.232250588815205 2213 7 -1.1381739990738637 2213 8 -0.9705289068892782 2213 9 -0.9376192215916035 2213 10 -1.0448245047581466 2213 11 -1.0415514234218861 2213 12 -1.0083516389672766 2213 13 -1.2170628756329478 2213 14 -6.327508116995556 2213 15 -2.8560417298334926 2213 16 -2.0581634260944455 2213 17 -0.9707047017804484 2213 18 -0.350533284526513 2213 19 -1.009645441343146 2213 20 -1.043853383657229 2213 21 -1.2884821663457586 2213 22 -1.1711831666466566 2213 23 -5.623870944278931 2213 24 -3.2334937496174163 2213 25 -2.2829852761775946 2214 1 -0.18294648279651327 2214 2 -0.20520083577542536 2214 3 -0.350051207838348 2214 4 -0.02762827331097384 2214 5 -0.1771567951175328 2214 6 -0.5613481934629974 2214 7 -0.7441684282776393 2214 8 -0.7348726552221336 2214 9 -0.5998245175298251 2214 10 -0.10638984289435882 2214 11 -0.5904938813196949 2214 12 -0.5513849502849197 2214 13 -0.5747859122353489 2214 14 -0.07299651592888523 2214 15 -0.4882885550281557 2214 16 -0.06625986533828154 2214 17 0.19851029984630486 2214 18 0.7868697205776698 2214 19 -0.2231985408593289 2214 20 -0.2683935149549015 2214 21 -0.37380987328009496 2214 22 0.0758003456299079 2214 23 1.510835927697201 2214 24 0.4651594611794394 2214 25 0.23129899973536538 2215 1 -2.6022012434859008 2215 2 -4.657621441300623 2215 3 -2.519056898494224 2215 4 -4.286829398723679 2215 5 -4.796278441552094 2215 6 -2.4564468847782877 2215 7 -1.8767699339840505 2215 8 -2.3414575856687208 2215 9 -1.8128550195140747 2215 10 -3.286851179605531 2215 11 -2.2768482522624574 2215 12 -1.995333307568508 2215 13 -1.9863378348153873 2215 14 -1.2585525450851165 2215 15 -1.8469963814130281 2215 16 -2.6619984331812887 2215 17 -7.270407364103075 2215 18 -3.413644976416247 2215 19 -2.408852393381224 2215 20 -2.1540401488858265 2215 21 -2.120394935494616 2215 22 -1.805071992443061 2215 23 -6.271695998812962 2215 24 -3.642230256224181 2215 25 -3.593891934860192 2216 1 -0.8636212175399791 2216 2 -0.3460647397702452 2216 3 -0.6838746041641066 2216 4 -2.7931619264056096 2216 5 -1.0294693055728494 2216 6 -0.7275567458437919 2216 7 -0.8828962938572289 2216 8 -0.7091697151184206 2216 9 -0.8343101982018126 2216 10 -0.38557272387240005 2216 11 -0.9167775200168435 2216 12 -1.6713212174145013 2216 13 -0.9809968504852193 2216 14 -6.37932112575702 2216 15 -3.048480466657761 2216 16 -1.4680943792654446 2216 17 -0.6430570241684208 2216 18 0.0738339627077309 2216 19 -0.38918166974836765 2216 20 -1.0204851268425585 2216 21 -1.1122373113229145 2216 22 -0.6050655328772478 2216 23 0.5074309908678813 2216 24 -0.19307699764237896 2216 25 -0.17020066980806092 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 108059.0 3 3.0 4 912.0 5 5463.0 6 6344.0 7 5812.0 8 5265.0 9 5059.0 10 4820.0 11 4621.0 12 4620.0 13 4532.0 14 4570.0 15 4767.0 16 4847.0 17 5143.0 18 6001.0 19 6925.0 20 8719.0 21 10675.0 22 13586.0 23 16345.0 24 19384.0 25 22645.0 26 25788.0 27 30027.0 28 33663.0 29 40543.0 30 47940.0 31 53672.0 32 69926.0 33 91851.0 34 119636.0 35 157695.0 36 221644.0 37 310387.0 38 771415.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.860973597176425 18.42612172786327 12.343905453016891 25.368999221943415 2 13.805435861233025 20.00776995602205 42.24240930796332 23.94438487478161 3 18.981323223220112 24.51989540649847 29.321163314140104 27.177618056141316 4 10.51419657368017 16.490790061887907 40.06617180608722 32.9288415583447 5 12.164777832629534 37.93497639993323 36.78782492266494 13.112420844772293 6 31.761957436532988 37.127001487910285 18.402369648050623 12.708671427506099 7 28.077768160330248 31.36573673347094 22.224249284239963 18.33224582195885 8 25.00923310209148 36.88064972629179 20.72445227285237 17.385664898764364 9 26.33856493781782 15.263322612650407 20.037910820787026 38.36020162874475 10 13.507228618804398 29.592031982170962 35.66713503410647 21.23360436491817 11 34.53070616795564 22.74659193961704 24.103468495721586 18.61923339670573 12 22.614673788322065 25.075826276706763 32.22606772674753 20.08343220822364 13 28.70197550843587 21.785898331024498 25.8125497405833 23.699576419956333 14 20.321216899087716 21.872347754446896 28.90978725217595 28.89664809428944 15 23.434713882596718 30.18138768705064 23.455976321012216 22.927922109340425 16 21.899788618420292 28.498160953623003 28.050761763815483 21.551288664141214 17 21.509148182082917 28.300989750910908 28.490807426505576 21.69905464050059 18 21.106124316917455 27.717959428081635 30.113070663283086 21.062845591717828 19 22.88223704596851 27.404239299422606 28.033528342645774 21.679995311963108 20 23.013316027262388 27.52841607724521 28.29147530999559 21.166792585496815 21 22.90888703035856 27.02319374823543 27.600063213706722 22.46785600769929 22 22.442839390908375 27.83816336651026 27.748617184076675 21.97038005850469 23 21.197560557673064 27.21759211167667 29.468811344525502 22.116035986124764 24 22.24165326989903 27.663803509930922 28.204380287949483 21.89016293222057 25 22.3890277413999 27.171167411734938 28.051176645550235 22.38862820131493 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 185.0 1 185.0 2 339.5 3 494.0 4 494.0 5 494.0 6 955.0 7 1416.0 8 1416.0 9 1416.0 10 2453.5 11 3491.0 12 3491.0 13 3491.0 14 6635.5 15 9780.0 16 9780.0 17 9780.0 18 18016.0 19 26252.0 20 26252.0 21 26252.0 22 41806.0 23 57360.0 24 57360.0 25 57360.0 26 81845.5 27 106331.0 28 106331.0 29 106331.0 30 136199.0 31 166067.0 32 166067.0 33 166067.0 34 192697.5 35 219328.0 36 219328.0 37 219328.0 38 240984.0 39 262640.0 40 262640.0 41 262640.0 42 277166.5 43 291693.0 44 291693.0 45 291693.0 46 301349.0 47 311005.0 48 311005.0 49 311005.0 50 294860.5 51 278716.0 52 278716.0 53 278716.0 54 243964.0 55 209212.0 56 209212.0 57 209212.0 58 176279.5 59 143347.0 60 143347.0 61 143347.0 62 115090.5 63 86834.0 64 86834.0 65 86834.0 66 66165.5 67 45497.0 68 45497.0 69 45497.0 70 32999.0 71 20501.0 72 20501.0 73 20501.0 74 14358.5 75 8216.0 76 8216.0 77 8216.0 78 5753.5 79 3291.0 80 3291.0 81 3291.0 82 2228.0 83 1165.0 84 1165.0 85 1165.0 86 749.5 87 334.0 88 334.0 89 334.0 90 217.5 91 101.0 92 101.0 93 101.0 94 67.0 95 33.0 96 33.0 97 33.0 98 24.0 99 15.0 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12537145453964488 2 0.04611006770502338 3 0.03434955958006554 4 0.03031104546923096 5 0.03408328392440612 6 0.021302052452753822 7 0.020148191278229657 8 0.02396480900934805 9 0.028802150087160895 10 0.035459041478646466 11 0.021435190280583535 12 0.02028132910605937 13 0.01619843571928155 14 0.022012120867845618 15 0.02241153435133475 16 0.022455913627277987 17 0.02733763398103407 18 0.020636363313605265 19 0.033817008268746694 20 0.023521016249915677 21 0.02787018529235292 22 0.03705669541260301 23 0.02920156357065003 24 0.03971945196919723 25 0.03168680302347131 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2253304.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.41124284331895 #Duplication Level Percentage of deduplicated Percentage of total 1 88.05248512381655 69.92357279125126 2 8.648265781188842 13.735390682471053 3 1.8503019167188686 4.408043245260618 4 0.6049535877576043 1.9216046506542472 5 0.2678180221050118 1.0633880995599228 6 0.13848183834699052 0.659820893661726 7 0.09002488344978724 0.5004291517100776 8 0.06345958097184892 0.4031523356232605 9 0.0443484217306834 0.3169586958995901 >10 0.2153584328996674 2.958118442722236 >50 0.013953006516527438 0.7642099813407787 >100 0.008689821926124778 1.4706403708670797 >500 8.452648052335716E-4 0.47501083820851564 >1k 0.0010143177662787126 1.3996598207695534 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 4867 0.2159939360157351 No Hit CCCATGTACTCTGCGTTGATACCAC 3849 0.1708158331055197 No Hit GTATCAACGCAGAGTACTTTTTTTT 2271 0.10078533566709152 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 8.875855188647426E-5 0.0 9 0.0 0.0 0.0 8.875855188647426E-5 0.0 10 0.0 0.0 0.0 1.7751710377294851E-4 0.0 11 0.0 0.0 0.0 1.7751710377294851E-4 0.0 12 0.0 0.0 0.0 1.7751710377294851E-4 0.0 13 0.0 0.0 0.0 1.7751710377294851E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 655 0.0 13.345773 4 TAAGACG 55 0.0030652888 12.092958 4 GCGTGCG 100 1.9339423E-6 11.398389 9 GTAGGAC 1255 0.0 11.280545 3 GTCCTAC 1270 0.0 11.086528 1 TAAGACT 275 0.0 11.056419 4 GGCGTGC 130 3.2750904E-8 10.959989 8 TGTAGGA 1380 0.0 10.948236 2 GACCGGG 70 0.0014936136 10.855608 7 GTCCTAA 545 0.0 10.648068 1 GTTCTAG 225 0.0 10.570488 1 CTACACT 540 0.0 10.557344 4 CGTGCGC 100 2.4034322E-5 10.448523 10 GGACCTG 905 0.0 10.1811075 6 AGGACCT 1000 0.0 10.166271 5 TCCTACA 1525 0.0 10.156495 2 TGGCGAG 1035 0.0 10.095863 18 TACACGG 85 6.587816E-4 10.0600815 5 GTCTTAC 190 9.640644E-11 10.014147 1 CTGTAGG 1445 0.0 10.007216 1 >>END_MODULE