FastQCFastQC Report
Thu 26 May 2016
SRR1513041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2253304
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC55320.2455061545179878No Hit
CCCATGTACTCTGCGTTGATACCAC51740.2296183737303089No Hit
GTATCAACGCAGAGTACTTTTTTTT35960.1595878762918807No Hit
TATCAACGCAGAGTACTTTTTTTTT25010.11099256913403607No Hit
GGTATCAACGCAGAGTACTTTTTTT23050.10229423104916159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1900.012.98468518
TAGGACC6100.012.3328754
CCAACGA1103.896821E-812.07291219
CCTACAC3800.011.7782663
GGCGAGG4050.011.24249919
AATACCG600.005772862611.1099955
TGCGATC600.005916107411.07347312
TGTAGGA14000.010.9586092
GTAGGAC13000.010.9146563
GTCCTAC13550.010.9030211
CGTGCGC803.8330027E-410.66898610
TAGGACT3950.010.6077364
ATAGGAC3350.010.5177783
TAGGACA6200.010.444424
TCCTATA2800.010.2098822
TGGCGAG11550.010.187119518
GGACCTG9300.010.1372326
TGCACCG856.4512796E-410.0830225
TGTTCGT856.7013426E-410.041410
AGGACGT10650.010.0146455
Produced by FastQC (version 0.11.5)