FastQCFastQC Report
Thu 26 May 2016
SRR1513040_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513040_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432075
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5800.011.9579324
GTAGGAC9350.011.8882413
CTACGCT802.8653789E-511.8760294
GTCCTAC9300.010.9436641
TCCTATA3400.010.8982272
TACGCTG1053.4654895E-610.857865
TCTACGC803.7707397E-410.6879853
CGCAGAT1252.2478525E-710.6378578
TGTAGGA11100.010.5281562
TAGGACG6750.010.2749644
AGGACGT7050.010.1070515
GTCCTAA5700.09.8455291
GGACGTG7250.09.826826
GTATAGG3100.09.8186141
TCCTACA10950.09.6311692
GAGTAGG2303.6379788E-129.5117831
GTATAAG3700.09.5117831
AAACCCG1002.7544453E-49.5006285
TGGCGAG7400.09.50004118
GAGGCGT800.00451990269.4992596
Produced by FastQC (version 0.11.5)