##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513040_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2432075
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.245230513039278	31.0	30.0	34.0	24.0	34.0
2	28.922614228590813	31.0	30.0	34.0	16.0	34.0
3	29.308711285630583	31.0	30.0	34.0	23.0	34.0
4	32.169876340162205	35.0	33.0	37.0	19.0	37.0
5	32.41222125140055	35.0	33.0	37.0	22.0	37.0
6	32.87243444383911	37.0	33.0	37.0	26.0	37.0
7	32.874989052558	37.0	35.0	37.0	26.0	37.0
8	32.86748763915587	37.0	35.0	37.0	26.0	37.0
9	34.251662880462156	39.0	35.0	39.0	26.0	39.0
10	33.74479405445967	39.0	34.0	39.0	19.0	39.0
11	34.11979071369098	39.0	35.0	39.0	25.0	39.0
12	33.9729165424586	39.0	34.0	39.0	24.0	39.0
13	34.04753019540927	39.0	35.0	39.0	25.0	39.0
14	34.37522403708767	40.0	34.0	41.0	17.0	41.0
15	34.913331620118626	40.0	34.0	41.0	23.0	41.0
16	34.49033232938951	39.0	34.0	41.0	17.0	41.0
17	34.01289845091126	39.0	33.0	41.0	11.0	41.0
18	33.40826084721894	39.0	32.0	41.0	10.0	41.0
19	34.31970519001264	39.0	34.0	41.0	18.0	41.0
20	34.57766598480721	39.0	34.0	41.0	18.0	41.0
21	34.602248697100215	40.0	34.0	41.0	18.0	41.0
22	34.18376489211887	40.0	34.0	41.0	10.0	41.0
23	32.459639196982	39.0	31.0	41.0	2.0	41.0
24	33.57406371102865	39.0	33.0	41.0	2.0	41.0
25	33.31002045578364	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8409909588036939
1101	2	1.219604587349064
1101	3	0.9610407474934348
1101	4	0.887194589603368
1101	5	1.0975770524848585
1101	6	0.8045040107948509
1101	7	0.8250088968567297
1101	8	0.9012863077268136
1101	9	0.9905371479112617
1101	10	1.3088430168890426
1101	11	0.9530835341811397
1101	12	1.0282335262240636
1101	13	0.9520411706836569
1101	14	0.09645936632101382
1101	15	0.6281409964133076
1101	16	1.2696320970525932
1101	17	1.8839431225472936
1101	18	-4.4578466961426635
1101	19	-0.6810811215044907
1101	20	0.3430507874287585
1101	21	0.7747698673171399
1101	22	1.479962089303072
1101	23	3.227309534432287
1101	24	2.183724589170076
1101	25	2.3490010083558133
1102	1	1.1677817490501816
1102	2	1.45670728993872
1102	3	1.2388992758918
1102	4	1.8154414181082217
1102	5	1.5300593672275369
1102	6	1.1374992372131985
1102	7	1.0883188067006984
1102	8	1.032408177841475
1102	9	1.1604293371186785
1102	10	1.48799959698988
1102	11	1.1971971625040396
1102	12	1.1857133574494583
1102	13	1.1416668654149404
1102	14	2.045054044099899
1102	15	1.4692930521556349
1102	16	1.8154769547775516
1102	17	1.2496140294929532
1102	18	1.6621507593413796
1102	19	1.600690035884277
1102	20	1.5156857382725448
1102	21	1.5594001195205038
1102	22	1.937067388693258
1102	23	3.564810384056628
1102	24	2.413334040826882
1102	25	2.6143238743173356
1103	1	0.8492097067755395
1103	2	1.0975573639720722
1103	3	0.9098882283695602
1103	4	1.387402009718116
1103	5	1.2351075515736127
1103	6	0.8168501120860299
1103	7	0.8154732723423876
1103	8	0.7384689724920577
1103	9	0.9231729708121961
1103	10	1.2011853995278443
1103	11	0.8655912740649185
1103	12	1.0789514717037108
1103	13	0.8638494706455973
1103	14	1.5924276104330062
1103	15	1.1685326652287173
1103	16	1.3960259339125045
1103	17	1.7143861684042392
1103	18	1.7549742349587802
1103	19	1.344417081439211
1103	20	1.2911913735374867
1103	21	1.2087655417526335
1103	22	1.4259412087191734
1103	23	2.9546143534198706
1103	24	1.85776278253649
1103	25	2.046209300248975
1104	1	0.8756490549216487
1104	2	1.2539222036724311
1104	3	0.9711906598072346
1104	4	1.5985367595078444
1104	5	1.3841280283100659
1104	6	1.0120458912553545
1104	7	0.9476943209962414
1104	8	1.0371766370886064
1104	9	1.1543140736461837
1104	10	1.4885086382610524
1104	11	0.9718302360090334
1104	12	1.194642917394141
1104	13	1.125431120529754
1104	14	2.0493031316371884
1104	15	1.5107423343326332
1104	16	1.818387502440416
1104	17	2.1877322795308913
1104	18	2.825602949714977
1104	19	1.9743980007949844
1104	20	1.4188606739210314
1104	21	1.6167499416588456
1104	22	1.8846315781207323
1104	23	3.3713301020142765
1104	24	2.321796736187949
1104	25	2.4322734746006063
1105	1	0.6477698804105287
1105	2	1.0118762752530372
1105	3	0.6605790570113506
1105	4	0.9098405063220625
1105	5	0.990203965976491
1105	6	0.6520152475663252
1105	7	0.4568835412243004
1105	8	0.5265669950406462
1105	9	0.7340232036938374
1105	10	1.1962213452731447
1105	11	0.8487658531001685
1105	12	0.8132594486700029
1105	13	0.7555670458784789
1105	14	1.5144372870607725
1105	15	1.0221960783632937
1105	16	1.341538298311498
1105	17	1.8841937247491671
1105	18	1.7823900961719943
1105	19	1.3337610098147792
1105	20	1.107052034515533
1105	21	1.0581806153893396
1105	22	1.5764964699837307
1105	23	2.99710532397269
1105	24	2.046271387043518
1105	25	2.094301735280645
1106	1	-0.5366408242369687
1106	2	-0.025752873310494806
1106	3	-0.22199915799387782
1106	4	-2.6108289126142523
1106	5	-0.455398861007577
1106	6	-0.4715062218598476
1106	7	-0.4274670463750141
1106	8	-0.3646297621127488
1106	9	-0.31789126374141574
1106	10	-0.17516714812330036
1106	11	-0.2543802159340274
1106	12	-0.22700584022369696
1106	13	-0.23066343867594696
1106	14	0.48941534864537317
1106	15	-0.05024616506880619
1106	16	-1.09827813921747
1106	17	-6.043459143004906
1106	18	-7.024471494527642
1106	19	-2.8969062823591614
1106	20	-1.5108181216984136
1106	21	-1.194041470007086
1106	22	-1.4062296580970752
1106	23	-5.316385382245166
1106	24	-2.2540205414554677
1106	25	-0.8220761144185431
1107	1	0.5342938577955785
1107	2	0.8542000970188823
1107	3	0.6536069600340788
1107	4	0.9686502297518516
1107	5	0.9726059676388701
1107	6	0.5357471052377178
1107	7	0.5048402853209666
1107	8	0.5105369646594369
1107	9	0.36591803391935684
1107	10	0.8421122379829526
1107	11	0.5615192967246969
1107	12	0.6694932937841713
1107	13	0.6705814128485983
1107	14	1.442724484062964
1107	15	0.8879444633086493
1107	16	-5.588169429330257
1107	17	-0.9166046421953595
1107	18	0.8241761869518598
1107	19	0.6362487456642825
1107	20	0.4971499373864674
1107	21	0.5962896562442452
1107	22	0.6921943307608345
1107	23	2.5959229393149954
1107	24	1.5280220710852817
1107	25	1.3125634874336143
1108	1	0.45937190477985723
1108	2	0.6578202355184466
1108	3	0.5036544508069198
1108	4	1.0209021392838125
1108	5	0.7395401521837215
1108	6	0.37831927017698774
1108	7	0.31469445281272357
1108	8	0.3872212220746363
1108	9	0.5282474703736071
1108	10	0.9258979247532437
1108	11	0.45570442810441136
1108	12	0.4692468159194192
1108	13	0.33542626376210904
1108	14	0.9710530623806619
1108	15	0.6717917626931964
1108	16	0.9195183750584661
1108	17	1.011320521500835
1108	18	0.8011368240833008
1108	19	0.819587932835482
1108	20	0.6690173381604936
1108	21	0.7078282216159053
1108	22	1.0933770024323408
1108	23	2.7222429135188264
1108	24	1.5559102069158754
1108	25	1.7041322068080262
1109	1	0.41162985245476236
1109	2	0.761561842049332
1109	3	0.6321308097827085
1109	4	0.6335749541956304
1109	5	0.4068352958130461
1109	6	0.36689871906389726
1109	7	0.2744985819815966
1109	8	0.19265810040067777
1109	9	0.3211151809417174
1109	10	0.7464440226607962
1109	11	0.4708399774064702
1109	12	0.5475537730953164
1109	13	0.37197888140998714
1109	14	-0.5527597319131701
1109	15	0.10134889400745095
1109	16	-0.185442478637448
1109	17	0.905686936479519
1109	18	1.6707003636893276
1109	19	0.9271753502939006
1109	20	0.6854869266367132
1109	21	0.6273817308521998
1109	22	0.38393145898133696
1109	23	-4.330424050343982
1109	24	-1.3837681246992872
1109	25	-0.11117385877945196
1110	1	0.5480958228281665
1110	2	0.8843476977391411
1110	3	0.6963225634860315
1110	4	1.251135197215632
1110	5	0.9274844868841114
1110	6	0.7114208707466005
1110	7	0.5460550022150557
1110	8	0.46304754677927207
1110	9	0.5319155768731818
1110	10	0.8262255777157534
1110	11	0.5159645592891877
1110	12	0.6427831402876123
1110	13	0.5065416501996651
1110	14	1.320155484142532
1110	15	0.870652942065341
1110	16	1.2191346256017468
1110	17	1.5367610712327462
1110	18	2.273431266822648
1110	19	1.3617183225104341
1110	20	1.0225959443170964
1110	21	0.8716574493312805
1110	22	1.1980136488919086
1110	23	2.651303712643255
1110	24	1.6856840563369602
1110	25	1.9358907872714752
1111	1	0.5974900455732168
1111	2	0.8641635012360602
1111	3	0.7348188282785593
1111	4	1.382123845804493
1111	5	0.9964626587941439
1111	6	0.5766206012888375
1111	7	0.5049636982606671
1111	8	0.2457921953279225
1111	9	0.4139011186542163
1111	10	0.5896155172888413
1111	11	0.47658049148235193
1111	12	0.5717146598082223
1111	13	0.4752523752220341
1111	14	-0.169855475147223
1111	15	0.30408179214426667
1111	16	0.73295137421173
1111	17	1.29925214795783
1111	18	1.9268522744740437
1111	19	1.1311519584810341
1111	20	0.7750517301182853
1111	21	0.7610571014492393
1111	22	1.0340375381327007
1111	23	1.0069226108827252
1111	24	0.9658286385266024
1111	25	0.7442660977009012
1112	1	0.4553233758808126
1112	2	0.8257190931004565
1112	3	0.6458452187559587
1112	4	1.1589830098538982
1112	5	0.9183883587983814
1112	6	0.3203049806194187
1112	7	0.30010618021213986
1112	8	0.26940262564822604
1112	9	0.3722415624693056
1112	10	0.754132095301415
1112	11	0.4913830016337002
1112	12	0.4908778620061156
1112	13	0.3617255157554098
1112	14	0.760507794827177
1112	15	0.4273285288870028
1112	16	0.8817480386393513
1112	17	1.1088473648729078
1112	18	1.2828532185039592
1112	19	0.7622474567896447
1112	20	0.7005030187796351
1112	21	0.7125678518925085
1112	22	0.653769578871227
1112	23	2.400042690278063
1112	24	1.2623357278282015
1112	25	1.3878476019762687
1113	1	0.44529645591657996
1113	2	0.9422393077155569
1113	3	0.6044439717406114
1113	4	0.6714767338635355
1113	5	0.764477303560156
1113	6	0.31720250864592714
1113	7	0.24717485578499065
1113	8	0.2150097270225615
1113	9	0.3244495526403952
1113	10	0.6973165204260567
1113	11	0.3547549759166415
1113	12	0.3345812942915103
1113	13	0.2245760588621053
1113	14	0.9819222574142401
1113	15	0.6493364065355536
1113	16	0.9770449932589713
1113	17	1.4420973977846074
1113	18	1.8950973894498162
1113	19	0.8703982557304215
1113	20	0.7012907568504332
1113	21	0.6466150109128748
1113	22	0.8744038700656631
1113	23	0.6863971973196925
1113	24	0.696250972193738
1113	25	1.0474671467193701
1114	1	-0.2178819705810895
1114	2	-2.985722673587585
1114	3	-1.0306291698765868
1114	4	0.14190955882313716
1114	5	0.3695685782946114
1114	6	0.2534700386672526
1114	7	0.13458248739731005
1114	8	0.18975827838186632
1114	9	0.37658301719902454
1114	10	0.3728462601385516
1114	11	0.1500949990184708
1114	12	0.26902720304700267
1114	13	0.1477658125058383
1114	14	0.9345006291343765
1114	15	0.41998479535502753
1114	16	0.7157652508311543
1114	17	1.0235575187322539
1114	18	1.2331679456878035
1114	19	0.5932165402252494
1114	20	0.4403992073384657
1114	21	0.41843563372532344
1114	22	0.4713278485058723
1114	23	2.125464584286071
1114	24	0.9648677766482834
1114	25	0.8792787219477347
1115	1	0.26523209792823366
1115	2	0.515482986924539
1115	3	0.31670807041049187
1115	4	0.5061965181204613
1115	5	0.4410452950223842
1115	6	0.055585301569230694
1115	7	-0.19876631536442346
1115	8	-0.11238512232814912
1115	9	-0.08490160739868458
1115	10	0.2039313062177115
1115	11	0.13958546405749672
1115	12	-0.036416788009503875
1115	13	-0.05954814597319569
1115	14	0.7568506046442991
1115	15	0.06875645271393438
1115	16	0.6273543481817256
1115	17	1.1069634565227418
1115	18	1.5408117149267113
1115	19	0.673593398418717
1115	20	0.2946630592566919
1115	21	0.3688825346895399
1115	22	0.6312189691940233
1115	23	2.3224950651713954
1115	24	1.0741196279823626
1115	25	0.555509060336064
1116	1	0.2814513554992182
1116	2	0.24111448752991294
1116	3	0.33659976743833653
1116	4	0.9552926398519261
1116	5	0.3316711175385194
1116	6	0.1968710548161141
1116	7	0.1732888619604651
1116	8	0.1328784412687014
1116	9	0.16210400402955116
1116	10	0.5768680224383758
1116	11	0.11747411425413645
1116	12	0.1878336129485163
1116	13	-0.01743836217440986
1116	14	0.7962714633491572
1116	15	0.15129203633236443
1116	16	0.5266978166332166
1116	17	0.8071081003241574
1116	18	1.2304305330112086
1116	19	0.5308526100041888
1116	20	0.28682787806440757
1116	21	0.2576340454739352
1116	22	0.570623982683621
1116	23	1.8055753379842088
1116	24	0.8356222887352445
1116	25	1.0683532076981521
1201	1	1.117425571525903
1201	2	1.5517440937952003
1201	3	1.1459424829786435
1201	4	1.732937924728759
1201	5	1.5319973919920074
1201	6	1.1316029148283846
1201	7	1.0457930803634738
1201	8	1.0417493966023486
1201	9	1.206623328332853
1201	10	1.4991147772170947
1201	11	1.1659861762368777
1201	12	1.282357895050687
1201	13	1.220747972422302
1201	14	2.1908015580431126
1201	15	1.6381765213281838
1201	16	1.9807813333196265
1201	17	2.4304944650095806
1201	18	2.968564725240455
1201	19	2.0334712680818967
1201	20	1.7596946141402583
1201	21	1.7044052362175535
1201	22	1.938239640438212
1201	23	3.7131153807580475
1201	24	2.486556919302302
1201	25	2.55567449187064
1202	1	1.0014727731568982
1202	2	1.296993523999518
1202	3	1.072301980438347
1202	4	1.657024690250907
1202	5	1.3556730806802122
1202	6	0.9576319703764611
1202	7	0.9256891228894517
1202	8	0.8884523665771269
1202	9	1.0308194054878612
1202	10	1.5584507178439893
1202	11	1.0983198417860933
1202	12	1.1035091242347832
1202	13	0.9974132814900969
1202	14	1.8714876322161018
1202	15	1.3141057215206473
1202	16	1.5171891003099205
1202	17	2.039272221382454
1202	18	2.7032742181406206
1202	19	1.8922313580763372
1202	20	1.5177180821280487
1202	21	1.4490989451982017
1202	22	1.9334201644361926
1202	23	3.498705289722686
1202	24	2.30597801627151
1202	25	2.458340883498913
1203	1	0.9665021382622463
1203	2	1.2425485246004975
1203	3	0.9925194835690334
1203	4	1.6624285757191188
1203	5	1.4361136858058146
1203	6	0.9491733932189632
1203	7	0.9650321308902434
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2211	17	0.11590841930535589
2211	18	-5.470640968568521
2211	19	-1.9111213493095178
2211	20	-0.9487921618892017
2211	21	-0.7738325596633615
2211	22	-0.4332379972262359
2211	23	-4.758918370435399
2211	24	-2.052850264915733
2211	25	-1.3408270146576484
2212	1	0.5407173885273195
2212	2	0.8582871409362376
2212	3	0.5446339017161961
2212	4	1.096337081445867
2212	5	0.9108850415874059
2212	6	0.5943090529927133
2212	7	0.38679623211213965
2212	8	0.38497017955418045
2212	9	0.20150476456134214
2212	10	-3.2754798470657356
2212	11	-0.8410344213842436
2212	12	-0.009943134971194922
2212	13	0.277163710323002
2212	14	1.0361443494390343
2212	15	0.6825025287470439
2212	16	0.9649697791610947
2212	17	1.4564997714389776
2212	18	1.981551676043317
2212	19	1.1904789094528923
2212	20	0.7612365487337271
2212	21	0.6284996365825819
2212	22	0.8691135395090228
2212	23	2.1922762382364738
2212	24	1.314770421404873
2212	25	1.2243971182715327
2213	1	-1.4708690464661807
2213	2	-3.906438598382291
2213	3	-1.7165853320362743
2213	4	-3.6260738015850364
2213	5	-1.4392922091418079
2213	6	-0.9724356786458621
2213	7	-0.921662505884516
2213	8	-0.7914307566792544
2213	9	-0.7146291485011389
2213	10	-0.812126776722593
2213	11	-0.9255991329216542
2213	12	-0.7979406114014935
2213	13	-1.1232391322433983
2213	14	-6.354432821541003
2213	15	-2.861032189454342
2213	16	-2.0973506486440527
2213	17	-0.9917789300696995
2213	18	-0.2100401119768236
2213	19	-0.8440165677858218
2213	20	-0.9246864933148444
2213	21	-1.0853208676943709
2213	22	-0.8957239032736766
2213	23	-5.730070053427003
2213	24	-3.2246357749965817
2213	25	-2.3479966951591003
2214	1	-0.3146304365765289
2214	2	-0.16438743001008405
2214	3	-0.29249355678832956
2214	4	-0.01089993654625232
2214	5	-0.30046368265707457
2214	6	-0.49440473697814724
2214	7	-0.5513750807794864
2214	8	-0.6244758513916864
2214	9	-0.5795096682418617
2214	10	-0.08821399272918029
2214	11	-0.6530549739301037
2214	12	-0.5120221981624269
2214	13	-0.6338786942663432
2214	14	-0.12914509802840968
2214	15	-0.5845305332923303
2214	16	-0.15219449622599512
2214	17	0.2571834375385862
2214	18	0.6268272639762102
2214	19	-0.3190546413944375
2214	20	-0.4961461805837075
2214	21	-0.5927449549402226
2214	22	-0.0461146573282889
2214	23	1.3944758075534338
2214	24	0.33923010572138423
2214	25	0.2894361098507332
2215	1	-2.398935449689489
2215	2	-4.2510028278837595
2215	3	-2.3890585374592703
2215	4	-3.900289963353501
2215	5	-5.005326339506485
2215	6	-2.376057658366168
2215	7	-1.5722181347351238
2215	8	-2.0777220165583827
2215	9	-1.605003832808876
2215	10	-3.0795804228373314
2215	11	-1.913593403342702
2215	12	-1.6114533919644813
2215	13	-1.7126878906252045
2215	14	-0.9066259665688747
2215	15	-1.5230721059398604
2215	16	-2.321399488278665
2215	17	-6.960411003565788
2215	18	-2.918873684042616
2215	19	-2.104433627104555
2215	20	-1.9340015091675156
2215	21	-1.8753444163476303
2215	22	-1.6357008048614574
2215	23	-6.23620772875924
2215	24	-3.476002727723369
2215	25	-3.2629888316470534
2216	1	-1.3691643063193304
2216	2	-0.8530572241041376
2216	3	-1.0080694958293108
2216	4	-3.827033675019152
2216	5	-1.6433890118748273
2216	6	-1.338152456533276
2216	7	-1.4512286160463432
2216	8	-1.3274252941992124
2216	9	-1.3411775578897505
2216	10	-0.9886030444729315
2216	11	-1.4056836859013515
2216	12	-2.2794638428426417
2216	13	-1.606299456175968
2216	14	-7.358930571913746
2216	15	-3.766659366166401
2216	16	-2.1911099958281213
2216	17	-1.379708485106491
2216	18	-0.588648841286961
2216	19	-1.1115851911583334
2216	20	-1.635210935520739
2216	21	-1.8874448487438684
2216	22	-1.3149990431161456
2216	23	-0.16741276288128404
2216	24	-0.9313440591945294
2216	25	-0.9124273005185515
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	117564.0
3	5.0
4	997.0
5	5997.0
6	6916.0
7	6216.0
8	5720.0
9	5597.0
10	5352.0
11	5155.0
12	5054.0
13	4987.0
14	4976.0
15	5221.0
16	5228.0
17	5772.0
18	6552.0
19	7518.0
20	9384.0
21	11846.0
22	14364.0
23	17491.0
24	20617.0
25	24174.0
26	28117.0
27	32077.0
28	35752.0
29	43383.0
30	51288.0
31	57886.0
32	74840.0
33	98616.0
34	128799.0
35	170316.0
36	240665.0
37	333896.0
38	833737.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.47677996401565	17.72116363414834	12.18508028150288	25.616976120333135
2	14.687895285675207	19.458575603816545	41.01896548532597	24.834563625182284
3	19.141280675524772	23.727190653540603	28.956749386643743	28.174779284290878
4	11.077819313693595	15.964049794292338	38.73855940063248	34.219571491381586
5	12.99306874006706	37.16622849302267	36.08586174332786	13.754841023582406
6	33.12200845272106	35.97896566231145	17.75696514661075	13.14206073835674
7	29.06058862645739	30.844595525445364	21.5423942728859	18.552421575211348
8	26.041467892322977	35.63803274265824	20.059401999039302	18.261097365979484
9	26.719416779355974	15.053153162045582	19.2300605725604	38.99736948603804
10	14.384414954109007	28.497905445840814	34.6921920532389	22.42548754681128
11	35.47369862574286	22.043632263150688	23.447704657060807	19.034964454045642
12	23.295127655435806	24.251345684003088	31.20757275987668	21.245953900684427
13	29.26811816206058	21.058293529954828	25.219955217343927	24.453633090640672
14	21.377165031806168	20.730924936790696	27.799468992483973	30.092441038919166
15	23.95320298337561	29.045183269671753	22.94687301571635	24.054740731236286
16	23.28812699175149	27.3843339607689	26.421795441749868	22.905743605729747
17	22.48990533345507	27.327243220389867	27.414926370008086	22.767925076146973
18	22.137429066209258	26.815039382415947	28.79738155151873	22.250149999856067
19	23.847631676468616	26.55389934434766	26.95224279496027	22.646226184223462
20	23.961335491597247	26.46735625830734	27.21378327871873	22.357524971376684
21	24.04450019535668	26.037467354870554	26.62871948837114	23.28931296140163
22	23.666391228649612	26.480136586545598	26.863021479640963	22.990450705163827
23	22.343379722585745	25.959693253501815	28.615634924617122	23.081292099295318
24	23.421606940795684	26.423098802192012	26.968016910230418	23.187277346781887
25	23.563088859239713	26.11166589070641	27.06144947182606	23.263795778227816
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	222.0
1	222.0
2	351.0
3	480.0
4	480.0
5	480.0
6	875.5
7	1271.0
8	1271.0
9	1271.0
10	2204.5
11	3138.0
12	3138.0
13	3138.0
14	5846.0
15	8554.0
16	8554.0
17	8554.0
18	15958.0
19	23362.0
20	23362.0
21	23362.0
22	36989.0
23	50616.0
24	50616.0
25	50616.0
26	72067.5
27	93519.0
28	93519.0
29	93519.0
30	120938.5
31	148358.0
32	148358.0
33	148358.0
34	175522.0
35	202686.0
36	202686.0
37	202686.0
38	228789.0
39	254892.0
40	254892.0
41	254892.0
42	275596.5
43	296301.0
44	296301.0
45	296301.0
46	310370.5
47	324440.0
48	324440.0
49	324440.0
50	319669.5
51	314899.0
52	314899.0
53	314899.0
54	288889.5
55	262880.0
56	262880.0
57	262880.0
58	228426.0
59	193972.0
60	193972.0
61	193972.0
62	159961.0
63	125950.0
64	125950.0
65	125950.0
66	98435.5
67	70921.0
68	70921.0
69	70921.0
70	52230.0
71	33539.0
72	33539.0
73	33539.0
74	23840.0
75	14141.0
76	14141.0
77	14141.0
78	9728.5
79	5316.0
80	5316.0
81	5316.0
82	3566.0
83	1816.0
84	1816.0
85	1816.0
86	1209.5
87	603.0
88	603.0
89	603.0
90	371.0
91	139.0
92	139.0
93	139.0
94	89.5
95	40.0
96	40.0
97	40.0
98	30.0
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11044067308779539
2	0.04440652529218877
3	0.03207137937769189
4	0.02927541297040593
5	0.03170132500025698
6	0.017762610116875507
7	0.017022501362005695
8	0.020846396595499726
9	0.025122580512525312
10	0.03166020784720866
11	0.017844844422972152
12	0.01694026705590905
13	0.013568660505946567
14	0.0193661790857601
15	0.01920171047356681
16	0.0199007020753883
17	0.0243824717576555
18	0.01669356413761911
19	0.031084567704532134
20	0.02018852214672656
21	0.02569822065520183
22	0.032729253826465056
23	0.025533752043008544
24	0.03634756329471747
25	0.02791854691981127
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2432075.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.47497949837056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.50277856772574	62.18867721888351
2	10.900344391198805	16.23605850119419
3	3.0199099789121333	6.747232012992174
4	1.1180797509890803	3.3307586612981996
5	0.5022528844742288	1.8702636637107832
6	0.27951583943107466	1.2490161846659475
7	0.1774089162903276	0.92487677824852
8	0.11193728309029456	0.6669221490602392
9	0.07497655174639717	0.5025489438754303
>10	0.29122970310467416	3.6288182150978487
>50	0.012644113520484534	0.6539307544958641
>100	0.007642499199544595	1.086091322321833
>500	8.90101090234274E-4	0.478408523359061
>1k	3.894192269714959E-4	0.43639707079629364
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.1117153048322936E-5	0.0
3	0.0	0.0	0.0	4.1117153048322936E-5	0.0
4	0.0	0.0	0.0	4.1117153048322936E-5	0.0
5	0.0	0.0	0.0	4.1117153048322936E-5	0.0
6	0.0	0.0	0.0	4.1117153048322936E-5	0.0
7	0.0	0.0	0.0	4.1117153048322936E-5	0.0
8	0.0	0.0	0.0	4.1117153048322936E-5	0.0
9	0.0	0.0	0.0	4.1117153048322936E-5	0.0
10	0.0	0.0	0.0	4.1117153048322936E-5	0.0
11	0.0	0.0	0.0	4.1117153048322936E-5	0.0
12	0.0	0.0	0.0	4.1117153048322936E-5	4.1117153048322936E-5
13	0.0	0.0	0.0	4.1117153048322936E-5	4.1117153048322936E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	580	0.0	11.957932	4
GTAGGAC	935	0.0	11.888241	3
CTACGCT	80	2.8653789E-5	11.876029	4
GTCCTAC	930	0.0	10.943664	1
TCCTATA	340	0.0	10.898227	2
TACGCTG	105	3.4654895E-6	10.85786	5
TCTACGC	80	3.7707397E-4	10.687985	3
CGCAGAT	125	2.2478525E-7	10.637857	8
TGTAGGA	1110	0.0	10.528156	2
TAGGACG	675	0.0	10.274964	4
AGGACGT	705	0.0	10.107051	5
GTCCTAA	570	0.0	9.845529	1
GGACGTG	725	0.0	9.82682	6
GTATAGG	310	0.0	9.818614	1
TCCTACA	1095	0.0	9.631169	2
GAGTAGG	230	3.6379788E-12	9.511783	1
GTATAAG	370	0.0	9.511783	1
AAACCCG	100	2.7544453E-4	9.500628	5
TGGCGAG	740	0.0	9.500041	18
GAGGCGT	80	0.0045199026	9.499259	6
>>END_MODULE