FastQCFastQC Report
Thu 26 May 2016
SRR1513040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513040_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432075
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34040.13996278897649125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTG802.8068253E-511.900015
CTAGCAC2150.011.0697763
TAGGACC5300.010.9569894
AATCCCG1303.333662E-810.946897519
GTATTAA2850.010.6979631
GTCCTAC9650.010.5645841
AGCACCG1658.967618E-1010.3854635
AAGATCG750.002602663110.1546755
CTAGGAC3500.010.0640083
ACCGTGT1804.0381565E-1010.0143838
TTAGACA3050.09.9882044
GCACCGT1553.9355655E-89.8271056
CTACACT4750.09.820644
GTAGGAC9450.09.7718593
GTATTAG2251.8189894E-129.7396041
ATTATAC2251.8189894E-129.7315643
TCCTACA11550.09.64921952
CCTACAC3850.09.6436433
GACGTGA4200.09.4877017
GACCGCT800.0045655189.4877017
Produced by FastQC (version 0.11.5)