##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513040_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2432075 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.482029953845995 31.0 31.0 34.0 27.0 34.0 2 30.643807037200745 31.0 31.0 34.0 27.0 34.0 3 30.63920849480382 31.0 31.0 34.0 27.0 34.0 4 33.88953712364956 37.0 35.0 37.0 30.0 37.0 5 33.792782294953895 37.0 35.0 37.0 28.0 37.0 6 33.74248943803131 37.0 35.0 37.0 29.0 37.0 7 33.6678198657525 37.0 35.0 37.0 28.0 37.0 8 33.661892416969046 37.0 35.0 37.0 28.0 37.0 9 35.22540916706927 39.0 35.0 39.0 28.0 39.0 10 35.12375317373025 39.0 35.0 39.0 28.0 39.0 11 35.133543990214115 39.0 35.0 39.0 28.0 39.0 12 35.047787999958885 39.0 35.0 39.0 27.0 39.0 13 35.01336800879907 39.0 35.0 39.0 27.0 39.0 14 36.190593217725606 40.0 36.0 41.0 27.0 41.0 15 36.17942538778615 40.0 37.0 41.0 27.0 41.0 16 36.14263252572392 40.0 36.0 41.0 27.0 41.0 17 36.04755445452957 40.0 36.0 41.0 27.0 41.0 18 35.96800345384086 40.0 36.0 41.0 27.0 41.0 19 36.048708613015634 40.0 37.0 41.0 27.0 41.0 20 35.9392263807654 40.0 36.0 41.0 27.0 41.0 21 35.955905142727914 40.0 37.0 41.0 27.0 41.0 22 35.92088401879054 40.0 37.0 41.0 27.0 41.0 23 35.85426107336328 40.0 37.0 41.0 27.0 41.0 24 35.723519628300934 40.0 36.0 41.0 27.0 41.0 25 35.69492922710032 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7008222421627686 1101 2 0.5490639561843942 1101 3 0.618743808848496 1101 4 0.9294919256513126 1101 5 0.7464444284700207 1101 6 0.6835115229917577 1101 7 0.6551412450323539 1101 8 0.5683334537468809 1101 9 0.6616397649160461 1101 10 0.6716605350876961 1101 11 0.5691667680713977 1101 12 0.6424921916001054 1101 13 0.6292205388402934 1101 14 0.6912769102320908 1101 15 0.6108396627911574 1101 16 0.7239288287563639 1101 17 0.6172053157989623 1101 18 0.5591488882635005 1101 19 0.654281634732456 1101 20 0.7425626044823161 1101 21 0.7237428815342 1101 22 0.7788551413032891 1101 23 0.702601506678235 1101 24 0.8368783429347957 1101 25 0.8527239113333849 1102 1 0.6961763220152548 1102 2 0.6106695631821637 1102 3 0.6131724517326518 1102 4 0.9390778666777919 1102 5 0.7234666650995436 1102 6 0.6592693443284006 1102 7 0.622301739505005 1102 8 0.6684366286405279 1102 9 0.6417585554692664 1102 10 0.7265323245969739 1102 11 0.6751977065969328 1102 12 0.7338163507334343 1102 13 0.706870974632217 1102 14 0.7664564641749578 1102 15 0.6734961897360137 1102 16 0.7467044251055128 1102 17 0.730567423076451 1102 18 0.7132223794613921 1102 19 0.6631029138571449 1102 20 0.7732963487724049 1102 21 0.8392813342245091 1102 22 0.831058238990046 1102 23 0.7858067428040911 1102 24 0.9745876337293993 1102 25 0.9934779071819904 1103 1 0.15957195054674855 1103 2 0.2858978218814059 1103 3 0.4760067579152363 1103 4 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-1.647377171321736 2216 2 -1.1808897756475858 2216 3 -2.9792189553578226 2216 4 -1.3440275777286956 2216 5 -1.5705493985748262 2216 6 -1.4271919387344028 2216 7 -1.3921776814676363 2216 8 -1.4461228952789043 2216 9 -1.531755110612231 2216 10 -1.5980710185360039 2216 11 -1.4461681497637997 2216 12 -1.586106773299072 2216 13 -1.6047919176996572 2216 14 -1.7612814892782822 2216 15 -1.9609110154844842 2216 16 -1.8523438358208253 2216 17 -1.8348507894847614 2216 18 -1.898932517303848 2216 19 -1.7895345701893106 2216 20 -1.6816850917001531 2216 21 -1.8349885177433123 2216 22 -2.009825082846625 2216 23 -1.9614058109367676 2216 24 -2.0007358665495545 2216 25 -2.028229903470823 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66484.0 3 1.0 4 1143.0 5 8767.0 6 9684.0 7 7487.0 8 5917.0 9 5298.0 10 4739.0 11 4372.0 12 4009.0 13 3786.0 14 3804.0 15 3719.0 16 3614.0 17 3960.0 18 4286.0 19 5213.0 20 6476.0 21 8509.0 22 10864.0 23 13010.0 24 15037.0 25 17167.0 26 18698.0 27 20384.0 28 23283.0 29 27346.0 30 34700.0 31 43784.0 32 56066.0 33 73253.0 34 97960.0 35 132845.0 36 194318.0 37 324936.0 38 1167156.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.155217786068214 18.66329299983357 13.316110714715329 27.865378499382885 2 14.213728673667303 19.90305483026572 38.95215116896241 26.931065327104577 3 17.01308553395763 23.830926266665294 29.348560385678894 29.80742781369818 4 10.627344962634787 16.596404304965926 37.290790785646 35.48545994675329 5 13.39910808872395 37.396818268553794 35.381957467248334 13.82211617547392 6 33.287590385313834 36.401591441872746 17.27081751246409 13.040000660349325 7 28.769016388252368 30.948949588556474 21.46636146423157 18.81567255895959 8 25.927298437032793 36.1174942017499 19.650669569870406 18.3045377913469 9 26.292596231875354 14.76352667247703 19.039488929534908 39.90438816611271 10 14.420494374186884 28.884039664408444 34.22580719439606 22.469658767008607 11 35.50316490308744 21.90226424000796 23.35950947086044 19.235061386044165 12 23.030645955857256 24.461662331125673 31.06267769481355 21.445014018203516 13 29.10217240230769 20.90448913798846 25.35764658472698 24.63569187497687 14 21.448995283775282 20.793370147572194 27.14386746874011 30.61376709991242 15 23.874314679569782 29.03583525830217 22.914475464151174 24.17537459797687 16 23.329902388735206 27.63130353825928 25.9124455287856 23.126348544219912 17 22.739706196249458 27.56613545538183 26.652620392377173 23.04153795599154 18 22.53218451481399 27.30486138512425 27.626737391886813 22.536216708174944 19 23.753047179197377 26.61824906111715 26.884932508605313 22.743771251080165 20 23.965749411510746 26.59404006866565 26.948798865166573 22.491411654657032 21 23.921301769065508 26.033983326994438 26.56739615349033 23.47731875044972 22 23.62714965615781 26.565915935980595 26.637007493601146 23.169926914260454 23 23.35623022768334 26.41869327344451 26.52670861186927 23.69836788700288 24 23.30507342108585 26.422750689022962 26.99576121874932 23.276414671141872 25 23.429540618607568 26.323365850148534 26.770350420936857 23.47674311030704 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 146.0 1 146.0 2 253.0 3 360.0 4 360.0 5 360.0 6 890.5 7 1421.0 8 1421.0 9 1421.0 10 2161.0 11 2901.0 12 2901.0 13 2901.0 14 5460.5 15 8020.0 16 8020.0 17 8020.0 18 15287.0 19 22554.0 20 22554.0 21 22554.0 22 35926.0 23 49298.0 24 49298.0 25 49298.0 26 70442.5 27 91587.0 28 91587.0 29 91587.0 30 118521.5 31 145456.0 32 145456.0 33 145456.0 34 171496.0 35 197536.0 36 197536.0 37 197536.0 38 224246.5 39 250957.0 40 250957.0 41 250957.0 42 271103.5 43 291250.0 44 291250.0 45 291250.0 46 308803.0 47 326356.0 48 326356.0 49 326356.0 50 323490.0 51 320624.0 52 320624.0 53 320624.0 54 293630.5 55 266637.0 56 266637.0 57 266637.0 58 232275.0 59 197913.0 60 197913.0 61 197913.0 62 163940.0 63 129967.0 64 129967.0 65 129967.0 66 101495.5 67 73024.0 68 73024.0 69 73024.0 70 53728.5 71 34433.0 72 34433.0 73 34433.0 74 24188.5 75 13944.0 76 13944.0 77 13944.0 78 9605.0 79 5266.0 80 5266.0 81 5266.0 82 3499.0 83 1732.0 84 1732.0 85 1732.0 86 1150.0 87 568.0 88 568.0 89 568.0 90 339.0 91 110.0 92 110.0 93 110.0 94 61.5 95 13.0 96 13.0 97 13.0 98 7.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19107141021555668 2 0.06697984231571806 3 0.0 4 0.0 5 0.01821489880040706 6 0.3747828500354635 7 0.0011512802853530422 8 0.030344458949662326 9 0.03231808229598183 10 0.002919317866430928 11 0.0036594266213007412 12 0.009744765272452535 13 0.008429016374906203 14 0.001850271887174532 15 4.111715304832293E-5 16 0.01175950577182036 17 0.024176885992413886 18 0.06726766238705631 19 0.029933287419179097 20 0.0 21 0.0 22 0.0 23 5.345229896281981E-4 24 3.2893722438658344E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2432075.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.07198207492101 #Duplication Level Percentage of deduplicated Percentage of total 1 79.35089716590174 54.80923746672066 2 13.26334590266886 18.32251180885241 3 3.8625364211653426 8.003791393393863 4 1.517415909613143 4.192436980359959 5 0.7059465155782291 2.4380562534936194 6 0.38447485555769406 1.5933864198803334 7 0.22966836620122627 1.1104554491399248 8 0.15349940126568395 0.8482006314187545 9 0.10168008237604238 0.6320920344529043 >10 0.4010473043675255 4.585565603523449 >50 0.01785320678482164 0.8384505053462123 >100 0.00984842520665449 1.298187093431615 >500 0.0010123178773741531 0.4628889510931322 >1k 7.741254356291513E-4 0.8647394088930652 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3404 0.13996278897649125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 4.1117153048322936E-5 8 0.0 0.0 0.0 4.1117153048322936E-5 4.1117153048322936E-5 9 0.0 0.0 0.0 8.223430609664587E-5 4.1117153048322936E-5 10 0.0 0.0 0.0 1.233514591449688E-4 4.1117153048322936E-5 11 0.0 0.0 0.0 1.233514591449688E-4 4.1117153048322936E-5 12 0.0 0.0 0.0 1.233514591449688E-4 1.233514591449688E-4 13 0.0 0.0 0.0 1.233514591449688E-4 1.6446861219329175E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGACTG 80 2.8068253E-5 11.90001 5 CTAGCAC 215 0.0 11.069776 3 TAGGACC 530 0.0 10.956989 4 AATCCCG 130 3.333662E-8 10.9468975 19 GTATTAA 285 0.0 10.697963 1 GTCCTAC 965 0.0 10.564584 1 AGCACCG 165 8.967618E-10 10.385463 5 AAGATCG 75 0.0026026631 10.154675 5 CTAGGAC 350 0.0 10.064008 3 ACCGTGT 180 4.0381565E-10 10.014383 8 TTAGACA 305 0.0 9.988204 4 GCACCGT 155 3.9355655E-8 9.827105 6 CTACACT 475 0.0 9.82064 4 GTAGGAC 945 0.0 9.771859 3 GTATTAG 225 1.8189894E-12 9.739604 1 ATTATAC 225 1.8189894E-12 9.731564 3 TCCTACA 1155 0.0 9.6492195 2 CCTACAC 385 0.0 9.643643 3 GACGTGA 420 0.0 9.487701 7 GACCGCT 80 0.004565518 9.487701 7 >>END_MODULE